Streptococcus satellite phage Javan435

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZNK0|A0A4D5ZNK0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan435 OX=2558703 GN=JavanS435_0011 PE=4 SV=1
MM1 pKa = 7.88KK2 pKa = 9.34YY3 pKa = 8.99TYY5 pKa = 10.63LALFEE10 pKa = 4.3VDD12 pKa = 4.79KK13 pKa = 11.42EE14 pKa = 4.28NGGYY18 pKa = 9.12TITFPDD24 pKa = 3.82FQGAISEE31 pKa = 4.32ADD33 pKa = 3.59TLNEE37 pKa = 4.16AIYY40 pKa = 10.24NARR43 pKa = 11.84EE44 pKa = 3.69VLEE47 pKa = 4.72IYY49 pKa = 10.37TIMFEE54 pKa = 4.86DD55 pKa = 3.72EE56 pKa = 4.19GKK58 pKa = 10.45EE59 pKa = 3.99FPEE62 pKa = 4.4PSSFKK67 pKa = 10.98ALASQLGSDD76 pKa = 4.14EE77 pKa = 6.05DD78 pKa = 3.57ILQAISVDD86 pKa = 3.58TEE88 pKa = 3.93LVRR91 pKa = 11.84EE92 pKa = 4.14RR93 pKa = 11.84EE94 pKa = 3.77RR95 pKa = 11.84SKK97 pKa = 11.23VVNKK101 pKa = 9.34TVTLPSWLVEE111 pKa = 3.9VGKK114 pKa = 10.22EE115 pKa = 3.77NKK117 pKa = 9.75INFSQLLQKK126 pKa = 10.63AIRR129 pKa = 11.84EE130 pKa = 4.1EE131 pKa = 4.21LQVV134 pKa = 3.26

Molecular weight: 15.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZNZ5|A0A4D5ZNZ5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan435 OX=2558703 GN=JavanS435_0007 PE=4 SV=1
MM1 pKa = 7.37RR2 pKa = 11.84QRR4 pKa = 11.84SISSYY9 pKa = 10.2WLGCRR14 pKa = 11.84INEE17 pKa = 4.05ALALSWSDD25 pKa = 2.88IDD27 pKa = 4.45LDD29 pKa = 3.82NAVVHH34 pKa = 5.2VTKK37 pKa = 10.18TLNRR41 pKa = 11.84DD42 pKa = 3.49LEE44 pKa = 4.53INSPKK49 pKa = 10.62SKK51 pKa = 10.68ASYY54 pKa = 10.37RR55 pKa = 11.84DD56 pKa = 2.95IDD58 pKa = 3.41IDD60 pKa = 3.65QATVSMLKK68 pKa = 9.95QYY70 pKa = 11.3KK71 pKa = 9.6LRR73 pKa = 11.84QAKK76 pKa = 7.99EE77 pKa = 3.33AWKK80 pKa = 9.74IGQRR84 pKa = 11.84EE85 pKa = 4.21SVVFSDD91 pKa = 6.23FIHH94 pKa = 7.59DD95 pKa = 4.06YY96 pKa = 10.4PSSSRR101 pKa = 11.84LKK103 pKa = 10.58RR104 pKa = 11.84RR105 pKa = 11.84LQTHH109 pKa = 6.76FKK111 pKa = 10.46RR112 pKa = 11.84ADD114 pKa = 3.23VPNIGFHH121 pKa = 6.1GFRR124 pKa = 11.84HH125 pKa = 4.87THH127 pKa = 6.58ASLLLNSGIPYY138 pKa = 10.03KK139 pKa = 10.3EE140 pKa = 3.58LQYY143 pKa = 11.41RR144 pKa = 11.84LGHH147 pKa = 5.67STLSMTMDD155 pKa = 3.58IYY157 pKa = 11.68SHH159 pKa = 6.98LSKK162 pKa = 11.0EE163 pKa = 4.25NAKK166 pKa = 10.37KK167 pKa = 10.37AVSFYY172 pKa = 9.42EE173 pKa = 4.24TALKK177 pKa = 10.67ALL179 pKa = 4.15

Molecular weight: 20.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

2783

44

285

139.2

16.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.534 ± 0.259

0.503 ± 0.121

5.713 ± 0.455

9.989 ± 0.682

3.737 ± 0.334

4.348 ± 0.361

1.545 ± 0.313

6.216 ± 0.395

9.127 ± 0.585

10.995 ± 0.617

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.515 ± 0.296

5.246 ± 0.453

2.264 ± 0.233

4.384 ± 0.488

5.354 ± 0.379

5.785 ± 0.66

6.001 ± 0.391

5.605 ± 0.467

0.826 ± 0.106

4.312 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski