Proteome-pI 2.0 - Proteome Isoelectric Point Database

Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Geobacteraceae; Geobacter; Geobacter lovleyi

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 3644 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|B3E1S0|B3E1S0_GEOLS Mammalian cell entry related domain protein OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) OX=398767 GN=Glov_1854 PE=4 SV=1

MM1 pKa = 7.4AQAQQGDD8 pKa = 4.42TVRR11 pKa = 11.84VHH13 pKa = 4.87YY14 pKa = 9.72TGTLQDD20 pKa = 3.62GSIFDD25 pKa = 3.49SSEE28 pKa = 3.82TVEE31 pKa = 4.73QDD33 pKa = 3.21SCGCSCSSSGGCGTGSDD50 pKa = 4.45CGCEE54 pKa = 3.76PLEE57 pKa = 3.98FTIGGGNVIPGFEE70 pKa = 4.14KK71 pKa = 10.6AVLGLSVGEE80 pKa = 4.41SIKK83 pKa = 9.89VTIPADD89 pKa = 3.34EE90 pKa = 4.8AYY92 pKa = 10.24GPRR95 pKa = 11.84HH96 pKa = 5.17EE97 pKa = 4.51QMVAVVDD104 pKa = 4.33RR105 pKa = 11.84SEE107 pKa = 4.04LSGEE111 pKa = 3.91IEE113 pKa = 4.55PIEE116 pKa = 4.23GQQLEE121 pKa = 4.82VVLQDD126 pKa = 3.79DD127 pKa = 3.54SSMPVLITEE136 pKa = 4.31VTEE139 pKa = 4.28TTVTLDD145 pKa = 3.62ANHH148 pKa = 7.26PLAGQDD154 pKa = 3.19LTFEE158 pKa = 4.32IKK160 pKa = 10.46LVEE163 pKa = 4.2IVV165 pKa = 3.25

Molecular weight: 17.31 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|B3E3X1|B3E3X1_GEOLS Uncharacterized protein OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) OX=398767 GN=Glov_0659 PE=4 SV=1

MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNTSRR12 pKa = 11.84KK13 pKa = 7.49RR14 pKa = 11.84THH16 pKa = 6.14GFLVRR21 pKa = 11.84MASKK25 pKa = 10.63NGRR28 pKa = 11.84LVIKK32 pKa = 10.39RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.23RR41 pKa = 11.84LSVQVASKK49 pKa = 10.98

Molecular weight: 5.72 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
3644	0	3644	1204365	31	2547	330.5	36.46

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
9.663 ± 0.049	1.347 ± 0.021	5.103 ± 0.025	6.193 ± 0.042	3.818 ± 0.026	7.564 ± 0.035	2.093 ± 0.023	5.835 ± 0.029	4.711 ± 0.047	11.019 ± 0.063

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.46 ± 0.018	3.25 ± 0.027	4.527 ± 0.026	4.193 ± 0.03	6.001 ± 0.04	5.926 ± 0.034	5.525 ± 0.04	6.939 ± 0.03	1.078 ± 0.017	2.756 ± 0.025

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski