Herbert virus strain F23/CI/2004

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Peribunyaviridae; Herbevirus; Herbert herbevirus

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

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* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|U5F076|U5F076_9VIRU Replicase OS=Herbert virus strain F23/CI/2004 OX=1230253 PE=4 SV=1
MM1 pKa = 7.59EE2 pKa = 5.14NYY4 pKa = 10.24EE5 pKa = 4.16IIKK8 pKa = 10.57KK9 pKa = 10.21KK10 pKa = 10.6FNEE13 pKa = 3.93RR14 pKa = 11.84SNNGFQNAEE23 pKa = 4.22IYY25 pKa = 10.81NSLIKK30 pKa = 10.25CRR32 pKa = 11.84HH33 pKa = 6.6DD34 pKa = 3.12IFGEE38 pKa = 4.3QICSALDD45 pKa = 3.1IPIRR49 pKa = 11.84NDD51 pKa = 2.38VDD53 pKa = 4.05FEE55 pKa = 4.83VIIEE59 pKa = 4.23DD60 pKa = 4.71LLNKK64 pKa = 10.17YY65 pKa = 9.29DD66 pKa = 4.43FRR68 pKa = 11.84LEE70 pKa = 4.17KK71 pKa = 10.36YY72 pKa = 10.73FKK74 pKa = 9.81VTPDD78 pKa = 2.87NYY80 pKa = 10.68KK81 pKa = 10.0IEE83 pKa = 4.96DD84 pKa = 4.41NILLIIDD91 pKa = 4.0YY92 pKa = 9.73KK93 pKa = 10.67VSRR96 pKa = 11.84STMNIEE102 pKa = 3.74KK103 pKa = 9.55TLIKK107 pKa = 10.57YY108 pKa = 10.13NNAFNWVPKK117 pKa = 10.25LLPIDD122 pKa = 4.41FKK124 pKa = 11.17TIVINLNPDD133 pKa = 3.21TLMIWSNQMEE143 pKa = 4.56FLDD146 pKa = 3.65QYY148 pKa = 11.05RR149 pKa = 11.84FEE151 pKa = 5.22INLEE155 pKa = 4.17EE156 pKa = 4.15IKK158 pKa = 10.8SINDD162 pKa = 3.19MLEE165 pKa = 3.59VLEE168 pKa = 4.76IQNSDD173 pKa = 3.32DD174 pKa = 4.98DD175 pKa = 3.63IFMRR179 pKa = 11.84NRR181 pKa = 11.84IGDD184 pKa = 4.46SIQVTEE190 pKa = 4.72DD191 pKa = 2.8WFDD194 pKa = 3.53VDD196 pKa = 5.93DD197 pKa = 4.4SVVDD201 pKa = 3.55QTLKK205 pKa = 10.81NDD207 pKa = 3.14VTFFDD212 pKa = 5.16FEE214 pKa = 4.49SSLDD218 pKa = 3.63KK219 pKa = 10.97DD220 pKa = 3.22ILGIYY225 pKa = 7.61TKK227 pKa = 10.6SLNSNVGMSGIEE239 pKa = 3.53MQEE242 pKa = 3.89IYY244 pKa = 10.81KK245 pKa = 8.65EE246 pKa = 4.48TIKK249 pKa = 9.86KK250 pKa = 7.04TQEE253 pKa = 3.7NANKK257 pKa = 9.01TKK259 pKa = 10.92KK260 pKa = 10.4KK261 pKa = 9.67MLGNLSDD268 pKa = 4.29LEE270 pKa = 4.06KK271 pKa = 10.88LIPNKK276 pKa = 10.73DD277 pKa = 3.87NIDD280 pKa = 3.68SGWRR284 pKa = 11.84ILEE287 pKa = 4.24SKK289 pKa = 10.73LLEE292 pKa = 4.38TKK294 pKa = 10.49SLSNNLSDD302 pKa = 4.81SKK304 pKa = 10.47PTVHH308 pKa = 6.93LLVSTIDD315 pKa = 3.43IVDD318 pKa = 4.48PDD320 pKa = 4.17LEE322 pKa = 4.27MNNFKK327 pKa = 10.45KK328 pKa = 10.77CCLFGRR334 pKa = 11.84LIKK337 pKa = 10.82LLDD340 pKa = 3.29WSKK343 pKa = 11.61VIGYY347 pKa = 9.68KK348 pKa = 10.64NNLIRR353 pKa = 11.84NDD355 pKa = 3.23HH356 pKa = 5.37FQEE359 pKa = 4.05FFKK362 pKa = 10.98ILGEE366 pKa = 4.27DD367 pKa = 3.16MDD369 pKa = 4.03MAGYY373 pKa = 6.92EE374 pKa = 3.95TSYY377 pKa = 11.44NSMVQEE383 pKa = 4.65FKK385 pKa = 11.26SNRR388 pKa = 11.84TGFSNNTKK396 pKa = 8.51YY397 pKa = 10.38QKK399 pKa = 10.41KK400 pKa = 8.66SQSKK404 pKa = 9.61SVSPNLPGIMVNDD417 pKa = 3.55MQFLLRR423 pKa = 11.84NFPNRR428 pKa = 11.84KK429 pKa = 9.22LFTSFCGIDD438 pKa = 3.09ISKK441 pKa = 11.29SNDD444 pKa = 2.91FKK446 pKa = 11.28KK447 pKa = 11.01GLVPEE452 pKa = 4.09QTKK455 pKa = 10.17PFSLSPFDD463 pKa = 4.2EE464 pKa = 4.39SLKK467 pKa = 9.45EE468 pKa = 3.81QVNEE472 pKa = 3.52IFNNIKK478 pKa = 10.07LAQISPTQEE487 pKa = 3.37FSKK490 pKa = 9.9TVLKK494 pKa = 10.68DD495 pKa = 3.62YY496 pKa = 10.86INKK499 pKa = 8.2TRR501 pKa = 11.84DD502 pKa = 2.96IDD504 pKa = 3.43QDD506 pKa = 3.4NYY508 pKa = 10.63MLYY511 pKa = 11.04EE512 pKa = 4.34NIIKK516 pKa = 9.4TKK518 pKa = 10.08GYY520 pKa = 9.93KK521 pKa = 9.46ICTDD525 pKa = 3.18MSILIKK531 pKa = 10.71NALSVANFQNYY542 pKa = 6.31KK543 pKa = 8.76TYY545 pKa = 11.06RR546 pKa = 11.84LVFSANKK553 pKa = 10.06SSFLILLPSMSIKK566 pKa = 10.14YY567 pKa = 8.92AQSSICFISCCFVKK581 pKa = 10.55KK582 pKa = 10.45GDD584 pKa = 3.96SIEE587 pKa = 4.37YY588 pKa = 9.95YY589 pKa = 10.44AGCDD593 pKa = 3.11RR594 pKa = 11.84YY595 pKa = 10.34RR596 pKa = 11.84IPVTGTEE603 pKa = 4.11FEE605 pKa = 4.34MLISNPIRR613 pKa = 11.84LNKK616 pKa = 9.32EE617 pKa = 3.3RR618 pKa = 11.84AKK620 pKa = 10.69RR621 pKa = 11.84LVEE624 pKa = 3.98APFLMFLISSSFVTTGCDD642 pKa = 3.03LDD644 pKa = 5.62SIINYY649 pKa = 9.68SFYY652 pKa = 11.37SSLNITKK659 pKa = 10.47SLMTLTEE666 pKa = 3.86PARR669 pKa = 11.84YY670 pKa = 9.47ILMGSSAKK678 pKa = 10.37VSDD681 pKa = 3.56VKK683 pKa = 11.26SYY685 pKa = 10.43IGEE688 pKa = 4.05KK689 pKa = 10.43FEE691 pKa = 5.12PSLKK695 pKa = 9.29TAFSIWIYY703 pKa = 11.36NKK705 pKa = 10.12IKK707 pKa = 10.36DD708 pKa = 3.62ASYY711 pKa = 10.9VANEE715 pKa = 3.86KK716 pKa = 10.9LKK718 pKa = 11.34GIVCKK723 pKa = 10.65DD724 pKa = 3.15VFFDD728 pKa = 3.49EE729 pKa = 5.6DD730 pKa = 3.61KK731 pKa = 11.3VKK733 pKa = 10.67GRR735 pKa = 11.84GVKK738 pKa = 9.99SIKK741 pKa = 9.89NLPSIYY747 pKa = 10.32FKK749 pKa = 11.45GDD751 pKa = 3.37LDD753 pKa = 4.06LKK755 pKa = 10.96GYY757 pKa = 10.03LNEE760 pKa = 4.06VFIPFYY766 pKa = 10.64INSKK770 pKa = 9.46GLHH773 pKa = 5.43EE774 pKa = 4.21FHH776 pKa = 7.33HH777 pKa = 6.66NIIDD781 pKa = 3.9LTKK784 pKa = 9.83VPCQIEE790 pKa = 4.23IEE792 pKa = 4.51TKK794 pKa = 10.59KK795 pKa = 10.89DD796 pKa = 3.17MKK798 pKa = 10.66EE799 pKa = 3.61FWKK802 pKa = 10.81EE803 pKa = 3.53SDD805 pKa = 3.37SQYY808 pKa = 11.25CNLPVFLNYY817 pKa = 9.47SAKK820 pKa = 10.09YY821 pKa = 7.3YY822 pKa = 10.25QRR824 pKa = 11.84LVKK827 pKa = 10.54QGARR831 pKa = 11.84YY832 pKa = 9.25RR833 pKa = 11.84NNVEE837 pKa = 3.66TSNMFKK843 pKa = 10.74EE844 pKa = 4.96PIYY847 pKa = 10.02KK848 pKa = 9.81IPTMTSSKK856 pKa = 10.7SCVQVGDD863 pKa = 3.78FFDD866 pKa = 4.1VKK868 pKa = 10.5IHH870 pKa = 5.54STDD873 pKa = 3.01KK874 pKa = 10.8KK875 pKa = 10.34VQLALKK881 pKa = 9.21TKK883 pKa = 9.0MPNTRR888 pKa = 11.84SYY890 pKa = 9.66ITIDD894 pKa = 3.82YY895 pKa = 10.52DD896 pKa = 4.42PEE898 pKa = 5.16ILDD901 pKa = 5.34DD902 pKa = 3.85MVLYY906 pKa = 10.45IPIDD910 pKa = 3.42KK911 pKa = 10.58VFSSKK916 pKa = 10.58LFSSKK921 pKa = 9.02MVIMDD926 pKa = 4.49TIKK929 pKa = 9.96TSKK932 pKa = 9.82QFKK935 pKa = 8.59TWIQGSNIGDD945 pKa = 3.78TFRR948 pKa = 11.84LSGKK952 pKa = 8.31NGYY955 pKa = 10.26YY956 pKa = 9.89IFCICWDD963 pKa = 3.54NLKK966 pKa = 11.07DD967 pKa = 3.6KK968 pKa = 11.06FEE970 pKa = 4.36WDD972 pKa = 3.66SPSVGFFVNAMKK984 pKa = 10.71LYY986 pKa = 9.69NDD988 pKa = 3.58SSKK991 pKa = 9.76MQGITNIFPSGFTKK1005 pKa = 10.58NVNQLEE1011 pKa = 4.19YY1012 pKa = 9.86MIEE1015 pKa = 4.0TGIMKK1020 pKa = 10.64ANLKK1024 pKa = 10.12VYY1026 pKa = 10.32NNSYY1030 pKa = 11.14YY1031 pKa = 10.86LFEE1034 pKa = 4.64QGLRR1038 pKa = 11.84DD1039 pKa = 3.45YY1040 pKa = 11.44SHH1042 pKa = 6.61TVRR1045 pKa = 11.84EE1046 pKa = 4.22NMEE1049 pKa = 4.04ITPKK1053 pKa = 11.04DD1054 pKa = 3.51SDD1056 pKa = 3.52NSNLLKK1062 pKa = 10.22TGLYY1066 pKa = 8.59QHH1068 pKa = 7.17HH1069 pKa = 8.17DD1070 pKa = 3.56YY1071 pKa = 11.08KK1072 pKa = 11.09AIRR1075 pKa = 11.84SNIKK1079 pKa = 10.42NYY1081 pKa = 9.37TDD1083 pKa = 4.35YY1084 pKa = 9.92ITKK1087 pKa = 10.22RR1088 pKa = 11.84VFDD1091 pKa = 4.13RR1092 pKa = 11.84LYY1094 pKa = 11.07DD1095 pKa = 4.22KK1096 pKa = 10.95IGEE1099 pKa = 4.22FQNKK1103 pKa = 7.79EE1104 pKa = 3.97FIKK1107 pKa = 10.64IAGEE1111 pKa = 4.48CILSHH1116 pKa = 4.96EE1117 pKa = 4.84TYY1119 pKa = 10.89YY1120 pKa = 10.34STLFCKK1126 pKa = 10.33DD1127 pKa = 2.54QRR1129 pKa = 11.84TAKK1132 pKa = 10.4DD1133 pKa = 3.29RR1134 pKa = 11.84EE1135 pKa = 4.16IYY1137 pKa = 10.62EE1138 pKa = 4.15MEE1140 pKa = 4.37LEE1142 pKa = 4.36GKK1144 pKa = 9.59ILLYY1148 pKa = 10.69VIEE1151 pKa = 5.05RR1152 pKa = 11.84LFKK1155 pKa = 9.13TYY1157 pKa = 10.64SRR1159 pKa = 11.84EE1160 pKa = 4.06DD1161 pKa = 3.54MNEE1164 pKa = 3.98MISRR1168 pKa = 11.84PGDD1171 pKa = 3.48VKK1173 pKa = 11.34VLDD1176 pKa = 4.19IEE1178 pKa = 4.26NSRR1181 pKa = 11.84NRR1183 pKa = 11.84LFSFATQFQSNEE1195 pKa = 3.63RR1196 pKa = 11.84YY1197 pKa = 9.76KK1198 pKa = 11.24YY1199 pKa = 8.93NVYY1202 pKa = 10.1MNEE1205 pKa = 3.64INADD1209 pKa = 3.48MSKK1212 pKa = 10.17WSAKK1216 pKa = 10.5DD1217 pKa = 2.84ITAKK1221 pKa = 10.69YY1222 pKa = 10.24LFLIALNPSLRR1233 pKa = 11.84SKK1235 pKa = 9.62EE1236 pKa = 3.94KK1237 pKa = 10.63KK1238 pKa = 10.28VISLGLCRR1246 pKa = 11.84YY1247 pKa = 6.26MRR1249 pKa = 11.84KK1250 pKa = 9.49RR1251 pKa = 11.84LILPDD1256 pKa = 3.43SAMGILLDD1264 pKa = 4.0QYY1266 pKa = 10.7SKK1268 pKa = 11.0RR1269 pKa = 11.84IDD1271 pKa = 3.55DD1272 pKa = 5.45PIIEE1276 pKa = 4.34MTNNLSTNCVTISQNWFQGNLNYY1299 pKa = 9.79MSSFCHH1305 pKa = 6.44SIAMDD1310 pKa = 4.1FYY1312 pKa = 11.64KK1313 pKa = 10.77DD1314 pKa = 3.89LNFDD1318 pKa = 3.61FAKK1321 pKa = 10.32TMGIDD1326 pKa = 3.36NVLTVSLVHH1335 pKa = 7.3SDD1337 pKa = 3.82DD1338 pKa = 3.93NQTGVCMIEE1347 pKa = 4.92KK1348 pKa = 9.69IDD1350 pKa = 3.79TLLEE1354 pKa = 4.22TEE1356 pKa = 4.34KK1357 pKa = 8.48TTNIYY1362 pKa = 10.12NQSQDD1367 pKa = 3.39KK1368 pKa = 10.55KK1369 pKa = 9.87IATAIFKK1376 pKa = 10.59LLEE1379 pKa = 3.71LSMRR1383 pKa = 11.84QFGFILNTKK1392 pKa = 7.78KK1393 pKa = 9.27TYY1395 pKa = 9.27ISSIIKK1401 pKa = 9.78EE1402 pKa = 4.59FISMHH1407 pKa = 5.91NLNGEE1412 pKa = 4.1PFSVYY1417 pKa = 10.2HH1418 pKa = 6.63RR1419 pKa = 11.84FLFPVVGACSFLGPYY1434 pKa = 10.01EE1435 pKa = 4.75DD1436 pKa = 3.27LTARR1440 pKa = 11.84LSGIQTAIRR1449 pKa = 11.84HH1450 pKa = 5.52GCPPSLAICSIGCATEE1466 pKa = 4.01MTYY1469 pKa = 8.91GTYY1472 pKa = 11.3SMLPGMKK1479 pKa = 9.37NDD1481 pKa = 4.0PGHH1484 pKa = 6.24IFGYY1488 pKa = 10.81DD1489 pKa = 3.04RR1490 pKa = 11.84FLLPLEE1496 pKa = 4.17IGGYY1500 pKa = 9.88PFFDD1504 pKa = 3.36LSNFVEE1510 pKa = 4.84LGTVANDD1517 pKa = 2.97IRR1519 pKa = 11.84LLRR1522 pKa = 11.84PMIAKK1527 pKa = 9.7LVNTIKK1533 pKa = 10.91YY1534 pKa = 7.99KK1535 pKa = 10.32TIEE1538 pKa = 4.06EE1539 pKa = 3.89QLLNFNSSDD1548 pKa = 2.85IKK1550 pKa = 11.08LLNEE1554 pKa = 3.8YY1555 pKa = 10.02EE1556 pKa = 4.0KK1557 pKa = 10.97FIIKK1561 pKa = 9.89FYY1563 pKa = 11.32KK1564 pKa = 9.78EE1565 pKa = 3.71FCITDD1570 pKa = 3.8LFDD1573 pKa = 4.3PSGSIGEE1580 pKa = 4.34TYY1582 pKa = 10.81EE1583 pKa = 3.65MSKK1586 pKa = 10.69RR1587 pKa = 11.84SLLTPRR1593 pKa = 11.84KK1594 pKa = 8.2FTTLKK1599 pKa = 10.48LIKK1602 pKa = 10.26KK1603 pKa = 6.67LTSYY1607 pKa = 11.01KK1608 pKa = 10.41HH1609 pKa = 4.71FTSMSQAEE1617 pKa = 4.18KK1618 pKa = 9.32LQNYY1622 pKa = 8.85EE1623 pKa = 4.1FMLSNKK1629 pKa = 8.99EE1630 pKa = 3.94LLVSKK1635 pKa = 10.21PLDD1638 pKa = 3.42FEE1640 pKa = 4.58AFKK1643 pKa = 10.85QVILFRR1649 pKa = 11.84YY1650 pKa = 8.71NSRR1653 pKa = 11.84KK1654 pKa = 9.58FKK1656 pKa = 10.62EE1657 pKa = 4.26SLSIQSPVQLFLEE1670 pKa = 4.57QIISSGSKK1678 pKa = 10.41CIDD1681 pKa = 2.91RR1682 pKa = 11.84EE1683 pKa = 4.06FLNDD1687 pKa = 3.83IDD1689 pKa = 4.35NEE1691 pKa = 4.27PNIDD1695 pKa = 3.51LAEE1698 pKa = 4.53PNRR1701 pKa = 11.84NQLLGRR1707 pKa = 11.84LTFVEE1712 pKa = 4.46AFQRR1716 pKa = 11.84IRR1718 pKa = 11.84SIIQRR1723 pKa = 11.84KK1724 pKa = 8.86EE1725 pKa = 3.39ISFDD1729 pKa = 3.41DD1730 pKa = 3.47MMVVYY1735 pKa = 10.41KK1736 pKa = 10.64SKK1738 pKa = 9.15ITNDD1742 pKa = 3.75PLTSTCYY1749 pKa = 9.31NIEE1752 pKa = 4.08YY1753 pKa = 10.29AVEE1756 pKa = 4.04EE1757 pKa = 4.38LSHH1760 pKa = 5.7SAKK1763 pKa = 10.33EE1764 pKa = 4.14SVYY1767 pKa = 9.58SQKK1770 pKa = 10.5LQEE1773 pKa = 4.04YY1774 pKa = 8.21RR1775 pKa = 11.84QVRR1778 pKa = 11.84SFLSTPHH1785 pKa = 7.04SIIMSLLKK1793 pKa = 10.58YY1794 pKa = 9.56IEE1796 pKa = 4.55SGIEE1800 pKa = 3.74PSEE1803 pKa = 4.09DD1804 pKa = 3.1TVLYY1808 pKa = 10.69SDD1810 pKa = 3.5MMEE1813 pKa = 3.96LLKK1816 pKa = 11.04YY1817 pKa = 10.35FIEE1820 pKa = 5.05SGLQAYY1826 pKa = 9.68LRR1828 pKa = 11.84DD1829 pKa = 3.85YY1830 pKa = 10.83NYY1832 pKa = 11.51KK1833 pKa = 10.53HH1834 pKa = 6.65MNDD1837 pKa = 2.82QNLYY1841 pKa = 10.07VKK1843 pKa = 9.63EE1844 pKa = 3.91QLSIWTEE1851 pKa = 3.91VLQILYY1857 pKa = 10.5LYY1859 pKa = 7.18TTQINKK1865 pKa = 10.05RR1866 pKa = 11.84NMSILLPKK1874 pKa = 9.43RR1875 pKa = 11.84TYY1877 pKa = 11.16SLTEE1881 pKa = 3.4FLITLKK1887 pKa = 10.92GALQRR1892 pKa = 11.84DD1893 pKa = 3.93KK1894 pKa = 11.48FLVNYY1899 pKa = 10.08KK1900 pKa = 9.11LTFNPLKK1907 pKa = 10.49FKK1909 pKa = 11.23AKK1911 pKa = 10.49GITKK1915 pKa = 10.28LEE1917 pKa = 3.93QLDD1920 pKa = 4.38TINTAEE1926 pKa = 4.09SFFRR1930 pKa = 11.84SMTTFMEE1937 pKa = 4.72SYY1939 pKa = 10.04IKK1941 pKa = 10.6PEE1943 pKa = 3.67FRR1945 pKa = 11.84ANLILQIIKK1954 pKa = 10.23NCSLGSTTISEE1965 pKa = 4.7LYY1967 pKa = 10.48DD1968 pKa = 3.32LVRR1971 pKa = 11.84INCPHH1976 pKa = 6.69QFMALLNLLGDD1987 pKa = 4.12LEE1989 pKa = 4.43EE1990 pKa = 4.52TTVKK1994 pKa = 10.63SYY1996 pKa = 11.17VSNIKK2001 pKa = 10.28SVSQAWLKK2009 pKa = 9.6EE2010 pKa = 3.71QSFRR2014 pKa = 11.84QGMIGEE2020 pKa = 4.48FDD2022 pKa = 3.6VIYY2025 pKa = 10.81YY2026 pKa = 9.58NLRR2029 pKa = 11.84SSLQVKK2035 pKa = 9.94GDD2037 pKa = 3.62NKK2039 pKa = 10.62KK2040 pKa = 8.68FTDD2043 pKa = 4.4MIFTYY2048 pKa = 10.59QRR2050 pKa = 11.84GLDD2053 pKa = 4.01LNQLIYY2059 pKa = 10.71KK2060 pKa = 10.32DD2061 pKa = 4.23LDD2063 pKa = 3.13IMLNKK2068 pKa = 9.8LRR2070 pKa = 11.84YY2071 pKa = 9.59DD2072 pKa = 3.45MKK2074 pKa = 10.87LDD2076 pKa = 3.71QIEE2079 pKa = 4.18FDD2081 pKa = 4.72PPISMDD2087 pKa = 3.74DD2088 pKa = 3.31NSIYY2092 pKa = 10.08FVKK2095 pKa = 10.45AWNKK2099 pKa = 10.45GIAKK2103 pKa = 8.18YY2104 pKa = 6.73TAKK2107 pKa = 10.36YY2108 pKa = 10.03YY2109 pKa = 11.01KK2110 pKa = 10.4DD2111 pKa = 3.28ILQTDD2116 pKa = 4.36LMLCPINIHH2125 pKa = 6.5ISNEE2129 pKa = 3.75TNYY2132 pKa = 10.43EE2133 pKa = 3.59INDD2136 pKa = 3.44IMNSNINNMSCRR2148 pKa = 11.84YY2149 pKa = 9.59FKK2151 pKa = 10.99INDD2154 pKa = 3.69ALKK2157 pKa = 10.36SYY2159 pKa = 7.79QTSRR2163 pKa = 11.84RR2164 pKa = 11.84GPISVLLNLKK2174 pKa = 10.42GDD2176 pKa = 3.5IDD2178 pKa = 4.15ALGTFSLSSYY2188 pKa = 10.28IRR2190 pKa = 11.84FIASFQGTQKK2200 pKa = 10.71PSFASLLNFIDD2211 pKa = 4.41LFKK2214 pKa = 11.35CRR2216 pKa = 11.84DD2217 pKa = 3.54TNKK2220 pKa = 9.5TLVEE2224 pKa = 4.07APKK2227 pKa = 10.17EE2228 pKa = 3.59IRR2230 pKa = 11.84TEE2232 pKa = 3.86IFCFDD2237 pKa = 4.39DD2238 pKa = 4.15ANLDD2242 pKa = 3.39IEE2244 pKa = 5.01FNFDD2248 pKa = 3.65VIFKK2252 pKa = 9.29MKK2254 pKa = 10.49VKK2256 pKa = 9.76TPYY2259 pKa = 10.59SSLTTLMDD2267 pKa = 3.33IVNNKK2272 pKa = 8.77EE2273 pKa = 3.85KK2274 pKa = 10.56LVEE2277 pKa = 3.93YY2278 pKa = 10.44MMTKK2282 pKa = 9.91RR2283 pKa = 11.84DD2284 pKa = 3.68DD2285 pKa = 3.52KK2286 pKa = 11.0KK2287 pKa = 9.44TVRR2290 pKa = 11.84DD2291 pKa = 3.87YY2292 pKa = 11.46QKK2294 pKa = 11.18SLAILIRR2301 pKa = 11.84QFQVIQNMKK2310 pKa = 8.62MKK2312 pKa = 10.62LGVSLEE2318 pKa = 3.96ADD2320 pKa = 3.65SIFVKK2325 pKa = 10.12MLHH2328 pKa = 6.7LYY2330 pKa = 10.76SLDD2333 pKa = 3.75TPAHH2337 pKa = 6.38KK2338 pKa = 10.56GFHH2341 pKa = 7.43DD2342 pKa = 4.13INSLPEE2348 pKa = 3.83KK2349 pKa = 10.27FFKK2352 pKa = 10.18KK2353 pKa = 10.42DD2354 pKa = 3.18KK2355 pKa = 10.97LFEE2358 pKa = 5.68LDD2360 pKa = 4.06LEE2362 pKa = 4.5FVEE2365 pKa = 4.47KK2366 pKa = 10.99NVMIKK2371 pKa = 10.59LDD2373 pKa = 3.79LPNNFLEE2380 pKa = 4.55KK2381 pKa = 10.47KK2382 pKa = 10.57LEE2384 pKa = 3.88IEE2386 pKa = 3.68ILEE2389 pKa = 4.28VFKK2392 pKa = 10.96KK2393 pKa = 10.83LKK2395 pKa = 8.9TEE2397 pKa = 3.68YY2398 pKa = 10.46RR2399 pKa = 11.84NFVSKK2404 pKa = 10.35EE2405 pKa = 3.87FPDD2408 pKa = 4.67LSWADD2413 pKa = 3.7EE2414 pKa = 4.29VEE2416 pKa = 4.03KK2417 pKa = 11.17SGFSFFF2423 pKa = 4.91

Molecular weight:
282.23 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|U5F075|U5F075_9VIRU Envelopment polyprotein OS=Herbert virus strain F23/CI/2004 OX=1230253 PE=4 SV=1
MM1 pKa = 7.19ATNFEE6 pKa = 4.47FNDD9 pKa = 4.09NITSSSFRR17 pKa = 11.84GEE19 pKa = 3.75TFYY22 pKa = 11.37ASHH25 pKa = 6.7EE26 pKa = 4.14NDD28 pKa = 3.03VFLRR32 pKa = 11.84DD33 pKa = 3.57VNKK36 pKa = 10.32VKK38 pKa = 10.76DD39 pKa = 3.6ILAKK43 pKa = 10.28GVQLKK48 pKa = 10.23RR49 pKa = 11.84AIQALGEE56 pKa = 4.24GEE58 pKa = 4.12NLVMKK63 pKa = 10.49GYY65 pKa = 7.25TVKK68 pKa = 10.9AGIEE72 pKa = 3.69LTIYY76 pKa = 10.56KK77 pKa = 10.15GGPDD81 pKa = 4.21LDD83 pKa = 4.44DD84 pKa = 3.54NVYY87 pKa = 9.16TFSRR91 pKa = 11.84LQGMCACIVFMNSTKK106 pKa = 10.58YY107 pKa = 9.99PDD109 pKa = 4.96LIVAIAKK116 pKa = 10.47ANGFKK121 pKa = 10.09WEE123 pKa = 3.89SSYY126 pKa = 11.45TGVQRR131 pKa = 11.84SAYY134 pKa = 10.49LSFTPGSHH142 pKa = 6.75LFSHH146 pKa = 6.41VFEE149 pKa = 4.55YY150 pKa = 10.47WPIACALYY158 pKa = 10.13EE159 pKa = 4.14IKK161 pKa = 10.61KK162 pKa = 10.27SGKK165 pKa = 9.9QSDD168 pKa = 3.78VDD170 pKa = 3.98LKK172 pKa = 10.91VYY174 pKa = 9.35QSRR177 pKa = 11.84FRR179 pKa = 11.84SRR181 pKa = 11.84DD182 pKa = 3.03EE183 pKa = 3.99TGPMLNKK190 pKa = 9.32IDD192 pKa = 4.62EE193 pKa = 4.43NTTLIKK199 pKa = 10.15NTFSSIIGEE208 pKa = 4.06GGIRR212 pKa = 11.84SVAIKK217 pKa = 10.73SLINKK222 pKa = 8.72IWPQQ226 pKa = 3.19

Molecular weight:
25.36 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3480

226

2423

1160.0

134.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.103 ± 0.695

2.155 ± 0.895

6.379 ± 0.289

5.69 ± 0.555

5.862 ± 0.504

4.339 ± 1.065

1.207 ± 0.161

8.937 ± 0.229

9.253 ± 0.292

9.511 ± 0.67

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.19 ± 0.254

6.667 ± 0.345

2.701 ± 0.282

3.276 ± 0.131

3.276 ± 0.409

7.902 ± 0.725

5.661 ± 0.413

5.086 ± 0.905

0.718 ± 0.139

5.086 ± 0.249

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski