Amino acid dipepetide frequency for Massilia glaciei

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.303AlaAla: 19.303 ± 0.195	1.392AlaCys: 1.392 ± 0.032	6.612AlaAsp: 6.612 ± 0.077	6.291AlaGlu: 6.291 ± 0.082	4.139AlaPhe: 4.139 ± 0.052	12.037AlaGly: 12.037 ± 0.162	2.785AlaHis: 2.785 ± 0.043	5.626AlaIle: 5.626 ± 0.064	4.335AlaLys: 4.335 ± 0.072	15.507AlaLeu: 15.507 ± 0.123	3.486AlaMet: 3.486 ± 0.051	3.407AlaAsn: 3.407 ± 0.059	7.224AlaPro: 7.224 ± 0.088	5.072AlaGln: 5.072 ± 0.068	9.664AlaArg: 9.664 ± 0.118	6.879AlaSer: 6.879 ± 0.088	6.053AlaThr: 6.053 ± 0.084	8.515AlaVal: 8.515 ± 0.081	1.68AlaTrp: 1.68 ± 0.037	2.613AlaTyr: 2.613 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	1.402CysAla: 1.402 ± 0.032	0.131CysCys: 0.131 ± 0.01	0.523CysAsp: 0.523 ± 0.019	0.471CysGlu: 0.471 ± 0.018	0.335CysPhe: 0.335 ± 0.015	1.0CysGly: 1.0 ± 0.024	0.254CysHis: 0.254 ± 0.014	0.42CysIle: 0.42 ± 0.014	0.293CysLys: 0.293 ± 0.012	0.857CysLeu: 0.857 ± 0.025	0.193CysMet: 0.193 ± 0.01	0.247CysAsn: 0.247 ± 0.013	0.441CysPro: 0.441 ± 0.018	0.254CysGln: 0.254 ± 0.013	0.548CysArg: 0.548 ± 0.016	0.518CysSer: 0.518 ± 0.022	0.432CysThr: 0.432 ± 0.017	0.709CysVal: 0.709 ± 0.022	0.125CysTrp: 0.125 ± 0.009	0.25CysTyr: 0.25 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.188AspAla: 7.188 ± 0.077	0.484AspCys: 0.484 ± 0.014	2.882AspAsp: 2.882 ± 0.049	3.006AspGlu: 3.006 ± 0.047	2.149AspPhe: 2.149 ± 0.037	5.112AspGly: 5.112 ± 0.074	0.987AspHis: 0.987 ± 0.027	2.602AspIle: 2.602 ± 0.052	2.072AspLys: 2.072 ± 0.043	5.045AspLeu: 5.045 ± 0.06	1.332AspMet: 1.332 ± 0.028	1.499AspAsn: 1.499 ± 0.031	2.923AspPro: 2.923 ± 0.048	1.815AspGln: 1.815 ± 0.031	2.82AspArg: 2.82 ± 0.047	2.537AspSer: 2.537 ± 0.037	2.578AspThr: 2.578 ± 0.048	3.628AspVal: 3.628 ± 0.058	0.898AspTrp: 0.898 ± 0.021	1.528AspTyr: 1.528 ± 0.028	0.0AspXaa: 0.0 ± 0.0
Glu	6.453GluAla: 6.453 ± 0.077	0.378GluCys: 0.378 ± 0.016	1.954GluAsp: 1.954 ± 0.035	2.246GluGlu: 2.246 ± 0.05	1.756GluPhe: 1.756 ± 0.035	3.389GluGly: 3.389 ± 0.048	1.293GluHis: 1.293 ± 0.026	2.739GluIle: 2.739 ± 0.051	2.039GluLys: 2.039 ± 0.043	6.001GluLeu: 6.001 ± 0.062	1.363GluMet: 1.363 ± 0.028	1.407GluAsn: 1.407 ± 0.032	2.187GluPro: 2.187 ± 0.04	2.66GluGln: 2.66 ± 0.041	4.661GluArg: 4.661 ± 0.062	2.43GluSer: 2.43 ± 0.034	2.478GluThr: 2.478 ± 0.038	3.603GluVal: 3.603 ± 0.047	0.677GluTrp: 0.677 ± 0.022	1.083GluTyr: 1.083 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	4.418PheAla: 4.418 ± 0.053	0.417PheCys: 0.417 ± 0.018	2.601PheAsp: 2.601 ± 0.04	2.035PheGlu: 2.035 ± 0.037	1.432PhePhe: 1.432 ± 0.034	3.576PheGly: 3.576 ± 0.05	0.733PheHis: 0.733 ± 0.021	1.582PheIle: 1.582 ± 0.036	1.426PheLys: 1.426 ± 0.028	3.104PheLeu: 3.104 ± 0.051	0.88PheMet: 0.88 ± 0.023	1.289PheAsn: 1.289 ± 0.03	1.509PhePro: 1.509 ± 0.035	0.936PheGln: 0.936 ± 0.022	1.852PheArg: 1.852 ± 0.035	2.471PheSer: 2.471 ± 0.044	2.042PheThr: 2.042 ± 0.05	2.685PheVal: 2.685 ± 0.036	0.501PheTrp: 0.501 ± 0.017	0.999PheTyr: 0.999 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	11.478GlyAla: 11.478 ± 0.143	0.843GlyCys: 0.843 ± 0.027	4.169GlyAsp: 4.169 ± 0.066	4.324GlyGlu: 4.324 ± 0.053	3.355GlyPhe: 3.355 ± 0.057	7.647GlyGly: 7.647 ± 0.138	1.892GlyHis: 1.892 ± 0.037	3.928GlyIle: 3.928 ± 0.06	3.857GlyLys: 3.857 ± 0.05	8.159GlyLeu: 8.159 ± 0.081	2.478GlyMet: 2.478 ± 0.033	2.578GlyAsn: 2.578 ± 0.066	3.092GlyPro: 3.092 ± 0.058	3.205GlyGln: 3.205 ± 0.049	5.419GlyArg: 5.419 ± 0.062	4.587GlySer: 4.587 ± 0.087	4.331GlyThr: 4.331 ± 0.068	6.479GlyVal: 6.479 ± 0.07	1.267GlyTrp: 1.267 ± 0.028	2.271GlyTyr: 2.271 ± 0.039	0.0GlyXaa: 0.0 ± 0.0
His	2.872HisAla: 2.872 ± 0.047	0.264HisCys: 0.264 ± 0.013	1.172HisAsp: 1.172 ± 0.028	1.136HisGlu: 1.136 ± 0.028	0.912HisPhe: 0.912 ± 0.023	2.072HisGly: 2.072 ± 0.038	0.585HisHis: 0.585 ± 0.02	0.937HisIle: 0.937 ± 0.024	0.667HisLys: 0.667 ± 0.02	2.183HisLeu: 2.183 ± 0.043	0.503HisMet: 0.503 ± 0.017	0.596HisAsn: 0.596 ± 0.019	1.308HisPro: 1.308 ± 0.031	0.741HisGln: 0.741 ± 0.019	1.283HisArg: 1.283 ± 0.034	1.027HisSer: 1.027 ± 0.024	1.036HisThr: 1.036 ± 0.024	1.514HisVal: 1.514 ± 0.027	0.361HisTrp: 0.361 ± 0.015	0.667HisTyr: 0.667 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.379IleAla: 6.379 ± 0.065	0.451IleCys: 0.451 ± 0.017	3.491IleAsp: 3.491 ± 0.051	3.176IleGlu: 3.176 ± 0.042	1.384IlePhe: 1.384 ± 0.033	4.55IleGly: 4.55 ± 0.056	0.839IleHis: 0.839 ± 0.02	1.913IleIle: 1.913 ± 0.038	1.743IleLys: 1.743 ± 0.034	3.618IleLeu: 3.618 ± 0.048	0.989IleMet: 0.989 ± 0.028	1.644IleAsn: 1.644 ± 0.036	1.91IlePro: 1.91 ± 0.038	1.004IleGln: 1.004 ± 0.026	2.446IleArg: 2.446 ± 0.046	2.674IleSer: 2.674 ± 0.045	2.522IleThr: 2.522 ± 0.059	3.698IleVal: 3.698 ± 0.057	0.464IleTrp: 0.464 ± 0.019	1.033IleTyr: 1.033 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	4.174LysAla: 4.174 ± 0.063	0.203LysCys: 0.203 ± 0.011	1.742LysAsp: 1.742 ± 0.035	1.601LysGlu: 1.601 ± 0.035	1.2LysPhe: 1.2 ± 0.03	2.595LysGly: 2.595 ± 0.043	0.8LysHis: 0.8 ± 0.024	1.872LysIle: 1.872 ± 0.039	1.65LysLys: 1.65 ± 0.04	4.043LysLeu: 4.043 ± 0.064	1.15LysMet: 1.15 ± 0.031	1.248LysAsn: 1.248 ± 0.031	2.19LysPro: 2.19 ± 0.051	1.375LysGln: 1.375 ± 0.03	2.46LysArg: 2.46 ± 0.038	2.023LysSer: 2.023 ± 0.034	2.049LysThr: 2.049 ± 0.033	2.584LysVal: 2.584 ± 0.05	0.435LysTrp: 0.435 ± 0.015	0.81LysTyr: 0.81 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	15.576LeuAla: 15.576 ± 0.133	1.063LeuCys: 1.063 ± 0.028	5.784LeuAsp: 5.784 ± 0.058	5.403LeuGlu: 5.403 ± 0.06	3.631LeuPhe: 3.631 ± 0.053	8.642LeuGly: 8.642 ± 0.078	2.208LeuHis: 2.208 ± 0.047	4.382LeuIle: 4.382 ± 0.058	3.639LeuLys: 3.639 ± 0.048	11.036LeuLeu: 11.036 ± 0.121	2.442LeuMet: 2.442 ± 0.041	2.869LeuAsn: 2.869 ± 0.042	5.647LeuPro: 5.647 ± 0.064	3.327LeuGln: 3.327 ± 0.041	7.688LeuArg: 7.688 ± 0.088	6.222LeuSer: 6.222 ± 0.07	5.22LeuThr: 5.22 ± 0.082	7.574LeuVal: 7.574 ± 0.073	1.234LeuTrp: 1.234 ± 0.031	2.171LeuTyr: 2.171 ± 0.043	0.0LeuXaa: 0.0 ± 0.0
Met	3.199MetAla: 3.199 ± 0.044	0.2MetCys: 0.2 ± 0.01	1.125MetAsp: 1.125 ± 0.027	1.03MetGlu: 1.03 ± 0.026	0.869MetPhe: 0.869 ± 0.027	1.807MetGly: 1.807 ± 0.03	0.603MetHis: 0.603 ± 0.022	1.088MetIle: 1.088 ± 0.031	1.016MetLys: 1.016 ± 0.026	3.073MetLeu: 3.073 ± 0.043	0.655MetMet: 0.655 ± 0.024	0.87MetAsn: 0.87 ± 0.022	1.507MetPro: 1.507 ± 0.031	1.097MetGln: 1.097 ± 0.023	1.975MetArg: 1.975 ± 0.039	1.633MetSer: 1.633 ± 0.035	1.464MetThr: 1.464 ± 0.027	1.807MetVal: 1.807 ± 0.034	0.234MetTrp: 0.234 ± 0.014	0.473MetTyr: 0.473 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.628AsnAla: 3.628 ± 0.068	0.299AsnCys: 0.299 ± 0.014	1.501AsnAsp: 1.501 ± 0.032	1.345AsnGlu: 1.345 ± 0.032	1.112AsnPhe: 1.112 ± 0.029	2.689AsnGly: 2.689 ± 0.056	0.579AsnHis: 0.579 ± 0.019	1.54AsnIle: 1.54 ± 0.036	1.116AsnLys: 1.116 ± 0.026	2.977AsnLeu: 2.977 ± 0.046	0.747AsnMet: 0.747 ± 0.021	1.014AsnAsn: 1.014 ± 0.037	1.823AsnPro: 1.823 ± 0.04	0.977AsnGln: 0.977 ± 0.024	1.699AsnArg: 1.699 ± 0.034	1.423AsnSer: 1.423 ± 0.03	1.59AsnThr: 1.59 ± 0.046	2.183AsnVal: 2.183 ± 0.051	0.45AsnTrp: 0.45 ± 0.016	0.855AsnTyr: 0.855 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	7.471ProAla: 7.471 ± 0.093	0.374ProCys: 0.374 ± 0.017	3.069ProAsp: 3.069 ± 0.051	2.987ProGlu: 2.987 ± 0.047	1.742ProPhe: 1.742 ± 0.035	4.739ProGly: 4.739 ± 0.06	1.075ProHis: 1.075 ± 0.028	1.901ProIle: 1.901 ± 0.034	1.589ProLys: 1.589 ± 0.036	4.946ProLeu: 4.946 ± 0.065	1.221ProMet: 1.221 ± 0.032	1.379ProAsn: 1.379 ± 0.031	2.596ProPro: 2.596 ± 0.049	1.683ProGln: 1.683 ± 0.032	2.774ProArg: 2.774 ± 0.043	2.619ProSer: 2.619 ± 0.038	2.297ProThr: 2.297 ± 0.046	3.952ProVal: 3.952 ± 0.056	0.62ProTrp: 0.62 ± 0.022	1.078ProTyr: 1.078 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	4.627GlnAla: 4.627 ± 0.063	0.315GlnCys: 0.315 ± 0.014	1.477GlnAsp: 1.477 ± 0.03	1.492GlnGlu: 1.492 ± 0.033	1.411GlnPhe: 1.411 ± 0.032	2.519GlnGly: 2.519 ± 0.04	0.82GlnHis: 0.82 ± 0.024	1.88GlnIle: 1.88 ± 0.034	1.227GlnLys: 1.227 ± 0.032	4.029GlnLeu: 4.029 ± 0.053	1.068GlnMet: 1.068 ± 0.023	0.99GlnAsn: 0.99 ± 0.022	1.71GlnPro: 1.71 ± 0.035	1.641GlnGln: 1.641 ± 0.037	2.673GlnArg: 2.673 ± 0.046	1.957GlnSer: 1.957 ± 0.031	1.705GlnThr: 1.705 ± 0.032	2.608GlnVal: 2.608 ± 0.04	0.537GlnTrp: 0.537 ± 0.02	0.856GlnTyr: 0.856 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	8.565ArgAla: 8.565 ± 0.093	0.573ArgCys: 0.573 ± 0.021	3.612ArgAsp: 3.612 ± 0.052	3.793ArgGlu: 3.793 ± 0.056	2.832ArgPhe: 2.832 ± 0.047	4.592ArgGly: 4.592 ± 0.059	1.816ArgHis: 1.816 ± 0.037	3.474ArgIle: 3.474 ± 0.05	2.251ArgLys: 2.251 ± 0.04	7.386ArgLeu: 7.386 ± 0.091	1.854ArgMet: 1.854 ± 0.029	1.968ArgAsn: 1.968 ± 0.034	3.047ArgPro: 3.047 ± 0.044	2.725ArgGln: 2.725 ± 0.047	4.844ArgArg: 4.844 ± 0.077	3.209ArgSer: 3.209 ± 0.045	2.904ArgThr: 2.904 ± 0.04	4.763ArgVal: 4.763 ± 0.061	0.991ArgTrp: 0.991 ± 0.027	1.842ArgTyr: 1.842 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	6.908SerAla: 6.908 ± 0.098	0.471SerCys: 0.471 ± 0.019	2.858SerAsp: 2.858 ± 0.04	2.601SerGlu: 2.601 ± 0.038	2.093SerPhe: 2.093 ± 0.037	5.362SerGly: 5.362 ± 0.081	1.183SerHis: 1.183 ± 0.029	2.605SerIle: 2.605 ± 0.043	1.939SerLys: 1.939 ± 0.032	5.522SerLeu: 5.522 ± 0.06	1.46SerMet: 1.46 ± 0.029	1.669SerAsn: 1.669 ± 0.042	2.615SerPro: 2.615 ± 0.043	1.664SerGln: 1.664 ± 0.03	3.1SerArg: 3.1 ± 0.044	3.134SerSer: 3.134 ± 0.052	2.889SerThr: 2.889 ± 0.051	4.193SerVal: 4.193 ± 0.067	0.746SerTrp: 0.746 ± 0.023	1.455SerTyr: 1.455 ± 0.028	0.0SerXaa: 0.0 ± 0.0
Thr	5.626ThrAla: 5.626 ± 0.085	0.402ThrCys: 0.402 ± 0.018	2.565ThrAsp: 2.565 ± 0.05	2.279ThrGlu: 2.279 ± 0.038	1.789ThrPhe: 1.789 ± 0.042	4.614ThrGly: 4.614 ± 0.086	1.066ThrHis: 1.066 ± 0.027	2.555ThrIle: 2.555 ± 0.052	1.45ThrLys: 1.45 ± 0.03	6.184ThrLeu: 6.184 ± 0.084	1.241ThrMet: 1.241 ± 0.029	1.381ThrAsn: 1.381 ± 0.036	3.066ThrPro: 3.066 ± 0.046	1.49ThrGln: 1.49 ± 0.028	3.117ThrArg: 3.117 ± 0.043	2.695ThrSer: 2.695 ± 0.055	2.685ThrThr: 2.685 ± 0.059	4.156ThrVal: 4.156 ± 0.071	0.628ThrTrp: 0.628 ± 0.025	1.134ThrTyr: 1.134 ± 0.027	0.0ThrXaa: 0.0 ± 0.0
Val	9.496ValAla: 9.496 ± 0.078	0.724ValCys: 0.724 ± 0.02	3.994ValAsp: 3.994 ± 0.051	3.866ValGlu: 3.866 ± 0.051	2.714ValPhe: 2.714 ± 0.045	5.592ValGly: 5.592 ± 0.074	1.487ValHis: 1.487 ± 0.031	3.296ValIle: 3.296 ± 0.048	2.585ValLys: 2.585 ± 0.045	7.825ValLeu: 7.825 ± 0.066	1.786ValMet: 1.786 ± 0.036	2.297ValAsn: 2.297 ± 0.044	3.564ValPro: 3.564 ± 0.05	2.278ValGln: 2.278 ± 0.036	4.961ValArg: 4.961 ± 0.069	4.24ValSer: 4.24 ± 0.065	3.886ValThr: 3.886 ± 0.075	5.732ValVal: 5.732 ± 0.084	0.908ValTrp: 0.908 ± 0.025	1.623ValTyr: 1.623 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.144TrpAla: 1.144 ± 0.026	0.142TrpCys: 0.142 ± 0.009	0.64TrpAsp: 0.64 ± 0.02	0.575TrpGlu: 0.575 ± 0.021	0.568TrpPhe: 0.568 ± 0.018	0.82TrpGly: 0.82 ± 0.022	0.342TrpHis: 0.342 ± 0.013	0.685TrpIle: 0.685 ± 0.024	0.441TrpLys: 0.441 ± 0.017	1.672TrpLeu: 1.672 ± 0.04	0.374TrpMet: 0.374 ± 0.015	0.473TrpAsn: 0.473 ± 0.018	0.608TrpPro: 0.608 ± 0.02	0.666TrpGln: 0.666 ± 0.02	1.236TrpArg: 1.236 ± 0.031	0.865TrpSer: 0.865 ± 0.023	0.69TrpThr: 0.69 ± 0.023	0.839TrpVal: 0.839 ± 0.023	0.229TrpTrp: 0.229 ± 0.012	0.33TrpTyr: 0.33 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.718TyrAla: 2.718 ± 0.037	0.277TyrCys: 0.277 ± 0.014	1.314TyrAsp: 1.314 ± 0.026	1.138TyrGlu: 1.138 ± 0.026	1.065TyrPhe: 1.065 ± 0.024	2.0TyrGly: 2.0 ± 0.036	0.489TyrHis: 0.489 ± 0.015	0.905TyrIle: 0.905 ± 0.025	0.868TyrLys: 0.868 ± 0.024	2.589TyrLeu: 2.589 ± 0.036	0.503TyrMet: 0.503 ± 0.016	0.724TyrAsn: 0.724 ± 0.023	1.142TyrPro: 1.142 ± 0.027	0.932TyrGln: 0.932 ± 0.022	1.823TyrArg: 1.823 ± 0.034	1.324TyrSer: 1.324 ± 0.027	1.271TyrThr: 1.271 ± 0.031	1.633TyrVal: 1.633 ± 0.034	0.355TyrTrp: 0.355 ± 0.013	0.694TyrTyr: 0.694 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5316 proteins (1765612 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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