Amino acid dipepetide frequency for Helicobacter sp. MIT 99-5507

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.743AlaAla: 2.743 ± 0.095
0.752AlaCys: 0.752 ± 0.048
2.269AlaAsp: 2.269 ± 0.069
2.094AlaGlu: 2.094 ± 0.074
3.125AlaPhe: 3.125 ± 0.103
3.216AlaGly: 3.216 ± 0.092
0.938AlaHis: 0.938 ± 0.039
6.445AlaIle: 6.445 ± 0.117
5.874AlaLys: 5.874 ± 0.119
6.84AlaLeu: 6.84 ± 0.128
1.673AlaMet: 1.673 ± 0.066
3.949AlaAsn: 3.949 ± 0.088
1.545AlaPro: 1.545 ± 0.057
1.796AlaGln: 1.796 ± 0.069
2.31AlaArg: 2.31 ± 0.06
3.853AlaSer: 3.853 ± 0.103
2.773AlaThr: 2.773 ± 0.083
2.509AlaVal: 2.509 ± 0.086
0.344AlaTrp: 0.344 ± 0.027
2.354AlaTyr: 2.354 ± 0.073
0.0AlaXaa: 0.0 ± 0.0
Cys
0.869CysAla: 0.869 ± 0.045
0.151CysCys: 0.151 ± 0.018
0.91CysAsp: 0.91 ± 0.041
0.596CysGlu: 0.596 ± 0.04
0.685CysPhe: 0.685 ± 0.037
1.035CysGly: 1.035 ± 0.048
0.22CysHis: 0.22 ± 0.024
1.34CysIle: 1.34 ± 0.057
1.007CysLys: 1.007 ± 0.054
0.914CysLeu: 0.914 ± 0.047
0.216CysMet: 0.216 ± 0.019
0.808CysAsn: 0.808 ± 0.045
0.339CysPro: 0.339 ± 0.03
0.179CysGln: 0.179 ± 0.019
0.274CysArg: 0.274 ± 0.023
0.78CysSer: 0.78 ± 0.048
0.32CysThr: 0.32 ± 0.026
0.73CysVal: 0.73 ± 0.042
0.061CysTrp: 0.061 ± 0.012
0.445CysTyr: 0.445 ± 0.037
0.0CysXaa: 0.0 ± 0.0
Asp
2.516AspAla: 2.516 ± 0.088
0.884AspCys: 0.884 ± 0.044
3.583AspAsp: 3.583 ± 0.096
4.236AspGlu: 4.236 ± 0.092
4.059AspPhe: 4.059 ± 0.092
3.235AspGly: 3.235 ± 0.093
0.411AspHis: 0.411 ± 0.03
7.337AspIle: 7.337 ± 0.152
5.317AspLys: 5.317 ± 0.125
5.206AspLeu: 5.206 ± 0.124
1.37AspMet: 1.37 ± 0.059
3.802AspAsn: 3.802 ± 0.112
1.035AspPro: 1.035 ± 0.053
0.45AspGln: 0.45 ± 0.032
1.96AspArg: 1.96 ± 0.063
5.969AspSer: 5.969 ± 0.116
2.686AspThr: 2.686 ± 0.077
2.954AspVal: 2.954 ± 0.092
0.322AspTrp: 0.322 ± 0.026
2.552AspTyr: 2.552 ± 0.074
0.0AspXaa: 0.0 ± 0.0
Glu
3.359GluAla: 3.359 ± 0.095
0.698GluCys: 0.698 ± 0.041
2.537GluAsp: 2.537 ± 0.083
3.132GluGlu: 3.132 ± 0.097
3.201GluPhe: 3.201 ± 0.087
2.427GluGly: 2.427 ± 0.078
0.875GluHis: 0.875 ± 0.052
7.484GluIle: 7.484 ± 0.148
4.357GluLys: 4.357 ± 0.112
5.922GluLeu: 5.922 ± 0.11
1.483GluMet: 1.483 ± 0.058
4.556GluAsn: 4.556 ± 0.112
1.096GluPro: 1.096 ± 0.054
1.805GluGln: 1.805 ± 0.064
1.686GluArg: 1.686 ± 0.071
4.577GluSer: 4.577 ± 0.113
1.688GluThr: 1.688 ± 0.07
3.449GluVal: 3.449 ± 0.087
0.406GluTrp: 0.406 ± 0.028
2.397GluTyr: 2.397 ± 0.068
0.0GluXaa: 0.0 ± 0.0
Phe
3.222PheAla: 3.222 ± 0.086
1.029PheCys: 1.029 ± 0.05
3.897PheAsp: 3.897 ± 0.097
2.851PheGlu: 2.851 ± 0.078
2.991PhePhe: 2.991 ± 0.096
3.936PheGly: 3.936 ± 0.087
0.72PheHis: 0.72 ± 0.039
6.116PheIle: 6.116 ± 0.142
4.061PheLys: 4.061 ± 0.087
5.412PheLeu: 5.412 ± 0.136
1.23PheMet: 1.23 ± 0.053
3.302PheAsn: 3.302 ± 0.072
1.184PhePro: 1.184 ± 0.06
0.929PheGln: 0.929 ± 0.049
1.699PheArg: 1.699 ± 0.066
4.439PheSer: 4.439 ± 0.094
1.811PheThr: 1.811 ± 0.06
3.179PheVal: 3.179 ± 0.088
0.424PheTrp: 0.424 ± 0.03
2.563PheTyr: 2.563 ± 0.084
0.0PheXaa: 0.0 ± 0.0
Gly
3.728GlyAla: 3.728 ± 0.103
0.696GlyCys: 0.696 ± 0.047
3.3GlyAsp: 3.3 ± 0.095
3.35GlyGlu: 3.35 ± 0.086
3.65GlyPhe: 3.65 ± 0.095
4.335GlyGly: 4.335 ± 0.137
0.919GlyHis: 0.919 ± 0.052
6.786GlyIle: 6.786 ± 0.119
4.238GlyLys: 4.238 ± 0.096
4.804GlyLeu: 4.804 ± 0.092
1.4GlyMet: 1.4 ± 0.061
3.125GlyAsn: 3.125 ± 0.101
0.802GlyPro: 0.802 ± 0.048
1.128GlyGln: 1.128 ± 0.054
1.82GlyArg: 1.82 ± 0.073
3.635GlySer: 3.635 ± 0.101
2.06GlyThr: 2.06 ± 0.069
4.024GlyVal: 4.024 ± 0.112
0.333GlyTrp: 0.333 ± 0.028
2.615GlyTyr: 2.615 ± 0.081
0.0GlyXaa: 0.0 ± 0.0
His
0.739HisAla: 0.739 ± 0.047
0.264HisCys: 0.264 ± 0.025
0.566HisAsp: 0.566 ± 0.034
0.482HisGlu: 0.482 ± 0.031
0.981HisPhe: 0.981 ± 0.046
0.839HisGly: 0.839 ± 0.042
0.35HisHis: 0.35 ± 0.026
1.895HisIle: 1.895 ± 0.07
1.485HisLys: 1.485 ± 0.061
1.66HisLeu: 1.66 ± 0.061
0.268HisMet: 0.268 ± 0.024
1.072HisAsn: 1.072 ± 0.049
0.627HisPro: 0.627 ± 0.044
0.447HisGln: 0.447 ± 0.03
0.527HisArg: 0.527 ± 0.037
1.178HisSer: 1.178 ± 0.054
0.897HisThr: 0.897 ± 0.044
0.372HisVal: 0.372 ± 0.027
0.084HisTrp: 0.084 ± 0.016
0.661HisTyr: 0.661 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
7.333IleAla: 7.333 ± 0.135
1.321IleCys: 1.321 ± 0.055
7.659IleAsp: 7.659 ± 0.123
6.786IleGlu: 6.786 ± 0.137
6.175IlePhe: 6.175 ± 0.156
6.17IleGly: 6.17 ± 0.119
1.422IleHis: 1.422 ± 0.058
10.307IleIle: 10.307 ± 0.191
10.188IleLys: 10.188 ± 0.178
11.543IleLeu: 11.543 ± 0.183
1.949IleMet: 1.949 ± 0.072
7.679IleAsn: 7.679 ± 0.184
3.406IlePro: 3.406 ± 0.079
2.544IleGln: 2.544 ± 0.078
3.004IleArg: 3.004 ± 0.089
7.696IleSer: 7.696 ± 0.136
4.925IleThr: 4.925 ± 0.115
5.373IleVal: 5.373 ± 0.121
0.718IleTrp: 0.718 ± 0.036
4.212IleTyr: 4.212 ± 0.106
0.0IleXaa: 0.0 ± 0.0
Lys
4.74LysAla: 4.74 ± 0.106
0.763LysCys: 0.763 ± 0.044
6.909LysAsp: 6.909 ± 0.133
7.378LysGlu: 7.378 ± 0.149
3.268LysPhe: 3.268 ± 0.096
3.426LysGly: 3.426 ± 0.094
1.279LysHis: 1.279 ± 0.053
10.689LysIle: 10.689 ± 0.173
7.884LysLys: 7.884 ± 0.15
7.087LysLeu: 7.087 ± 0.129
2.168LysMet: 2.168 ± 0.061
7.882LysAsn: 7.882 ± 0.146
2.282LysPro: 2.282 ± 0.072
2.991LysGln: 2.991 ± 0.081
2.784LysArg: 2.784 ± 0.072
5.507LysSer: 5.507 ± 0.118
3.601LysThr: 3.601 ± 0.097
4.096LysVal: 4.096 ± 0.103
0.478LysTrp: 0.478 ± 0.032
4.003LysTyr: 4.003 ± 0.107
0.0LysXaa: 0.0 ± 0.0
Leu
5.701LeuAla: 5.701 ± 0.133
1.223LeuCys: 1.223 ± 0.056
6.721LeuAsp: 6.721 ± 0.132
6.62LeuGlu: 6.62 ± 0.131
5.451LeuPhe: 5.451 ± 0.157
5.721LeuGly: 5.721 ± 0.13
1.705LeuHis: 1.705 ± 0.062
8.295LeuIle: 8.295 ± 0.139
9.17LeuLys: 9.17 ± 0.142
9.265LeuLeu: 9.265 ± 0.198
1.792LeuMet: 1.792 ± 0.069
6.881LeuAsn: 6.881 ± 0.131
3.088LeuPro: 3.088 ± 0.082
3.292LeuGln: 3.292 ± 0.078
3.235LeuArg: 3.235 ± 0.082
7.834LeuSer: 7.834 ± 0.128
3.123LeuThr: 3.123 ± 0.083
4.42LeuVal: 4.42 ± 0.104
0.614LeuTrp: 0.614 ± 0.038
3.674LeuTyr: 3.674 ± 0.104
0.0LeuXaa: 0.0 ± 0.0
Met
1.45MetAla: 1.45 ± 0.058
0.22MetCys: 0.22 ± 0.024
1.085MetAsp: 1.085 ± 0.051
1.135MetGlu: 1.135 ± 0.046
0.938MetPhe: 0.938 ± 0.04
1.271MetGly: 1.271 ± 0.065
0.38MetHis: 0.38 ± 0.025
2.297MetIle: 2.297 ± 0.071
1.882MetLys: 1.882 ± 0.061
2.563MetLeu: 2.563 ± 0.076
0.558MetMet: 0.558 ± 0.038
1.299MetAsn: 1.299 ± 0.052
1.027MetPro: 1.027 ± 0.048
1.357MetGln: 1.357 ± 0.052
0.83MetArg: 0.83 ± 0.041
1.247MetSer: 1.247 ± 0.05
0.823MetThr: 0.823 ± 0.047
1.111MetVal: 1.111 ± 0.052
0.166MetTrp: 0.166 ± 0.02
0.635MetTyr: 0.635 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
4.431AsnAla: 4.431 ± 0.11
0.538AsnCys: 0.538 ± 0.038
4.098AsnAsp: 4.098 ± 0.1
3.886AsnGlu: 3.886 ± 0.093
3.119AsnPhe: 3.119 ± 0.076
3.905AsnGly: 3.905 ± 0.104
1.128AsnHis: 1.128 ± 0.047
9.365AsnIle: 9.365 ± 0.168
6.92AsnLys: 6.92 ± 0.138
7.184AsnLeu: 7.184 ± 0.143
1.422AsnMet: 1.422 ± 0.054
5.578AsnAsn: 5.578 ± 0.157
2.546AsnPro: 2.546 ± 0.087
1.815AsnGln: 1.815 ± 0.056
2.129AsnArg: 2.129 ± 0.064
4.104AsnSer: 4.104 ± 0.107
3.551AsnThr: 3.551 ± 0.102
2.957AsnVal: 2.957 ± 0.075
0.35AsnTrp: 0.35 ± 0.027
2.47AsnTyr: 2.47 ± 0.086
0.0AsnXaa: 0.0 ± 0.0
Pro
1.225ProAla: 1.225 ± 0.052
0.272ProCys: 0.272 ± 0.025
1.161ProAsp: 1.161 ± 0.049
1.05ProGlu: 1.05 ± 0.056
1.679ProPhe: 1.679 ± 0.054
0.981ProGly: 0.981 ± 0.049
0.657ProHis: 0.657 ± 0.038
2.97ProIle: 2.97 ± 0.104
2.738ProLys: 2.738 ± 0.078
3.16ProLeu: 3.16 ± 0.078
0.59ProMet: 0.59 ± 0.035
2.204ProAsn: 2.204 ± 0.065
0.815ProPro: 0.815 ± 0.045
1.014ProGln: 1.014 ± 0.048
0.839ProArg: 0.839 ± 0.04
1.936ProSer: 1.936 ± 0.064
1.511ProThr: 1.511 ± 0.057
1.169ProVal: 1.169 ± 0.052
0.205ProTrp: 0.205 ± 0.022
1.351ProTyr: 1.351 ± 0.054
0.0ProXaa: 0.0 ± 0.0
Gln
1.576GlnAla: 1.576 ± 0.062
0.212GlnCys: 0.212 ± 0.023
1.939GlnAsp: 1.939 ± 0.061
1.757GlnGlu: 1.757 ± 0.068
0.977GlnPhe: 0.977 ± 0.044
1.297GlnGly: 1.297 ± 0.06
0.32GlnHis: 0.32 ± 0.03
2.918GlnIle: 2.918 ± 0.081
2.866GlnLys: 2.866 ± 0.095
1.681GlnLeu: 1.681 ± 0.06
0.674GlnMet: 0.674 ± 0.042
3.201GlnAsn: 3.201 ± 0.087
0.491GlnPro: 0.491 ± 0.035
0.629GlnGln: 0.629 ± 0.039
0.988GlnArg: 0.988 ± 0.053
2.142GlnSer: 2.142 ± 0.065
1.355GlnThr: 1.355 ± 0.058
1.39GlnVal: 1.39 ± 0.054
0.186GlnTrp: 0.186 ± 0.019
1.115GlnTyr: 1.115 ± 0.061
0.0GlnXaa: 0.0 ± 0.0
Arg
1.921ArgAla: 1.921 ± 0.07
0.305ArgCys: 0.305 ± 0.023
2.269ArgAsp: 2.269 ± 0.072
2.213ArgGlu: 2.213 ± 0.076
2.293ArgPhe: 2.293 ± 0.075
1.835ArgGly: 1.835 ± 0.061
0.478ArgHis: 0.478 ± 0.029
3.672ArgIle: 3.672 ± 0.086
2.271ArgLys: 2.271 ± 0.069
3.298ArgLeu: 3.298 ± 0.084
0.743ArgMet: 0.743 ± 0.046
1.919ArgAsn: 1.919 ± 0.052
0.698ArgPro: 0.698 ± 0.045
0.897ArgGln: 0.897 ± 0.039
1.02ArgArg: 1.02 ± 0.05
1.595ArgSer: 1.595 ± 0.065
0.975ArgThr: 0.975 ± 0.047
1.826ArgVal: 1.826 ± 0.067
0.223ArgTrp: 0.223 ± 0.02
1.439ArgTyr: 1.439 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
3.916SerAla: 3.916 ± 0.097
0.815SerCys: 0.815 ± 0.042
3.484SerAsp: 3.484 ± 0.095
2.715SerGlu: 2.715 ± 0.085
4.238SerPhe: 4.238 ± 0.099
4.748SerGly: 4.748 ± 0.124
1.251SerHis: 1.251 ± 0.053
8.161SerIle: 8.161 ± 0.164
6.864SerLys: 6.864 ± 0.117
7.184SerLeu: 7.184 ± 0.129
1.701SerMet: 1.701 ± 0.056
5.347SerAsn: 5.347 ± 0.116
1.9SerPro: 1.9 ± 0.067
2.109SerGln: 2.109 ± 0.066
2.166SerArg: 2.166 ± 0.073
5.433SerSer: 5.433 ± 0.152
2.967SerThr: 2.967 ± 0.077
3.272SerVal: 3.272 ± 0.081
0.473SerTrp: 0.473 ± 0.032
2.907SerTyr: 2.907 ± 0.07
0.0SerXaa: 0.0 ± 0.0
Thr
1.982ThrAla: 1.982 ± 0.072
0.406ThrCys: 0.406 ± 0.03
1.63ThrAsp: 1.63 ± 0.063
1.336ThrGlu: 1.336 ± 0.052
2.042ThrPhe: 2.042 ± 0.06
2.183ThrGly: 2.183 ± 0.078
0.882ThrHis: 0.882 ± 0.045
4.686ThrIle: 4.686 ± 0.11
4.277ThrLys: 4.277 ± 0.097
4.61ThrLeu: 4.61 ± 0.1
0.951ThrMet: 0.951 ± 0.046
3.065ThrAsn: 3.065 ± 0.09
1.893ThrPro: 1.893 ± 0.057
1.755ThrGln: 1.755 ± 0.06
1.522ThrArg: 1.522 ± 0.065
2.944ThrSer: 2.944 ± 0.073
2.423ThrThr: 2.423 ± 0.078
0.575ThrVal: 0.575 ± 0.039
0.305ThrTrp: 0.305 ± 0.023
1.617ThrTyr: 1.617 ± 0.065
0.0ThrXaa: 0.0 ± 0.0
Val
3.307ValAla: 3.307 ± 0.08
0.748ValCys: 0.748 ± 0.04
3.088ValAsp: 3.088 ± 0.082
2.937ValGlu: 2.937 ± 0.079
3.004ValPhe: 3.004 ± 0.088
3.242ValGly: 3.242 ± 0.094
0.635ValHis: 0.635 ± 0.042
4.982ValIle: 4.982 ± 0.106
3.363ValLys: 3.363 ± 0.091
4.917ValLeu: 4.917 ± 0.104
1.076ValMet: 1.076 ± 0.058
2.708ValAsn: 2.708 ± 0.081
1.277ValPro: 1.277 ± 0.051
1.135ValGln: 1.135 ± 0.047
1.522ValArg: 1.522 ± 0.065
3.581ValSer: 3.581 ± 0.098
1.66ValThr: 1.66 ± 0.059
3.35ValVal: 3.35 ± 0.103
0.333ValTrp: 0.333 ± 0.027
1.643ValTyr: 1.643 ± 0.064
0.0ValXaa: 0.0 ± 0.0
Trp
0.344TrpAla: 0.344 ± 0.029
0.121TrpCys: 0.121 ± 0.016
0.348TrpAsp: 0.348 ± 0.027
0.331TrpGlu: 0.331 ± 0.03
0.365TrpPhe: 0.365 ± 0.03
0.527TrpGly: 0.527 ± 0.035
0.182TrpHis: 0.182 ± 0.022
0.672TrpIle: 0.672 ± 0.039
0.365TrpLys: 0.365 ± 0.029
0.711TrpLeu: 0.711 ± 0.042
0.134TrpMet: 0.134 ± 0.017
0.426TrpAsn: 0.426 ± 0.034
0.065TrpPro: 0.065 ± 0.011
0.246TrpGln: 0.246 ± 0.024
0.253TrpArg: 0.253 ± 0.02
0.389TrpSer: 0.389 ± 0.029
0.212TrpThr: 0.212 ± 0.021
0.326TrpVal: 0.326 ± 0.023
0.091TrpTrp: 0.091 ± 0.015
0.246TrpTyr: 0.246 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.302TyrAla: 2.302 ± 0.069
0.465TyrCys: 0.465 ± 0.038
2.431TyrAsp: 2.431 ± 0.074
2.25TyrGlu: 2.25 ± 0.07
2.756TyrPhe: 2.756 ± 0.094
2.421TyrGly: 2.421 ± 0.071
0.642TyrHis: 0.642 ± 0.036
3.994TyrIle: 3.994 ± 0.096
4.083TyrLys: 4.083 ± 0.098
3.864TyrLeu: 3.864 ± 0.093
0.877TyrMet: 0.877 ± 0.046
2.693TyrAsn: 2.693 ± 0.087
1.411TyrPro: 1.411 ± 0.056
1.193TyrGln: 1.193 ± 0.048
1.325TyrArg: 1.325 ± 0.059
2.81TyrSer: 2.81 ± 0.071
1.72TyrThr: 1.72 ± 0.067
1.424TyrVal: 1.424 ± 0.06
0.24TyrTrp: 0.24 ± 0.027
1.714TyrTyr: 1.714 ± 0.061
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1538 proteins (462701 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski