Amino acid dipepetide frequency for Rhodobacter sp. ETT8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.036AlaAla: 20.036 ± 0.196
1.127AlaCys: 1.127 ± 0.032
7.221AlaAsp: 7.221 ± 0.082
8.952AlaGlu: 8.952 ± 0.107
4.442AlaPhe: 4.442 ± 0.057
11.669AlaGly: 11.669 ± 0.129
2.515AlaHis: 2.515 ± 0.051
5.919AlaIle: 5.919 ± 0.075
3.71AlaLys: 3.71 ± 0.07
15.388AlaLeu: 15.388 ± 0.147
3.951AlaMet: 3.951 ± 0.058
2.741AlaAsn: 2.741 ± 0.052
6.501AlaPro: 6.501 ± 0.084
5.066AlaGln: 5.066 ± 0.066
10.357AlaArg: 10.357 ± 0.114
5.991AlaSer: 5.991 ± 0.077
6.322AlaThr: 6.322 ± 0.096
9.187AlaVal: 9.187 ± 0.111
1.579AlaTrp: 1.579 ± 0.04
2.499AlaTyr: 2.499 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
1.092CysAla: 1.092 ± 0.027
0.103CysCys: 0.103 ± 0.009
0.582CysAsp: 0.582 ± 0.02
0.403CysGlu: 0.403 ± 0.018
0.309CysPhe: 0.309 ± 0.016
0.919CysGly: 0.919 ± 0.03
0.238CysHis: 0.238 ± 0.014
0.375CysIle: 0.375 ± 0.018
0.19CysLys: 0.19 ± 0.013
0.928CysLeu: 0.928 ± 0.027
0.172CysMet: 0.172 ± 0.012
0.183CysAsn: 0.183 ± 0.012
0.511CysPro: 0.511 ± 0.021
0.216CysGln: 0.216 ± 0.012
0.594CysArg: 0.594 ± 0.021
0.4CysSer: 0.4 ± 0.017
0.413CysThr: 0.413 ± 0.02
0.521CysVal: 0.521 ± 0.024
0.135CysTrp: 0.135 ± 0.009
0.19CysTyr: 0.19 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
7.248AspAla: 7.248 ± 0.09
0.513AspCys: 0.513 ± 0.02
2.922AspAsp: 2.922 ± 0.066
3.015AspGlu: 3.015 ± 0.061
2.131AspPhe: 2.131 ± 0.043
5.134AspGly: 5.134 ± 0.079
1.32AspHis: 1.32 ± 0.036
2.673AspIle: 2.673 ± 0.044
1.288AspLys: 1.288 ± 0.036
6.654AspLeu: 6.654 ± 0.075
1.435AspMet: 1.435 ± 0.037
1.052AspAsn: 1.052 ± 0.036
3.633AspPro: 3.633 ± 0.053
1.838AspGln: 1.838 ± 0.043
4.358AspArg: 4.358 ± 0.068
2.098AspSer: 2.098 ± 0.043
2.778AspThr: 2.778 ± 0.068
3.938AspVal: 3.938 ± 0.057
1.257AspTrp: 1.257 ± 0.033
1.383AspTyr: 1.383 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
8.663GluAla: 8.663 ± 0.1
0.319GluCys: 0.319 ± 0.017
2.804GluAsp: 2.804 ± 0.052
2.942GluGlu: 2.942 ± 0.062
1.653GluPhe: 1.653 ± 0.035
4.772GluGly: 4.772 ± 0.065
0.952GluHis: 0.952 ± 0.029
3.117GluIle: 3.117 ± 0.053
1.662GluLys: 1.662 ± 0.052
4.59GluLeu: 4.59 ± 0.071
1.608GluMet: 1.608 ± 0.037
1.381GluAsn: 1.381 ± 0.036
2.544GluPro: 2.544 ± 0.053
1.842GluGln: 1.842 ± 0.039
4.307GluArg: 4.307 ± 0.069
2.14GluSer: 2.14 ± 0.041
3.301GluThr: 3.301 ± 0.05
4.482GluVal: 4.482 ± 0.056
0.729GluTrp: 0.729 ± 0.023
0.901GluTyr: 0.901 ± 0.029
0.0GluXaa: 0.0 ± 0.0
Phe
4.788PheAla: 4.788 ± 0.067
0.38PheCys: 0.38 ± 0.019
2.733PheAsp: 2.733 ± 0.043
1.868PheGlu: 1.868 ± 0.039
1.321PhePhe: 1.321 ± 0.034
3.746PheGly: 3.746 ± 0.059
0.763PheHis: 0.763 ± 0.022
1.517PheIle: 1.517 ± 0.037
0.8PheLys: 0.8 ± 0.026
3.394PheLeu: 3.394 ± 0.065
0.788PheMet: 0.788 ± 0.028
0.967PheAsn: 0.967 ± 0.027
1.589PhePro: 1.589 ± 0.036
0.916PheGln: 0.916 ± 0.027
2.357PheArg: 2.357 ± 0.049
1.964PheSer: 1.964 ± 0.041
2.052PheThr: 2.052 ± 0.044
2.632PheVal: 2.632 ± 0.044
0.585PheTrp: 0.585 ± 0.02
0.802PheTyr: 0.802 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
10.814GlyAla: 10.814 ± 0.127
0.819GlyCys: 0.819 ± 0.023
4.433GlyAsp: 4.433 ± 0.093
4.358GlyGlu: 4.358 ± 0.064
3.817GlyPhe: 3.817 ± 0.06
7.734GlyGly: 7.734 ± 0.157
1.935GlyHis: 1.935 ± 0.041
4.272GlyIle: 4.272 ± 0.056
2.834GlyLys: 2.834 ± 0.05
9.813GlyLeu: 9.813 ± 0.106
2.576GlyMet: 2.576 ± 0.05
2.152GlyAsn: 2.152 ± 0.07
3.941GlyPro: 3.941 ± 0.062
3.402GlyGln: 3.402 ± 0.048
6.371GlyArg: 6.371 ± 0.065
4.283GlySer: 4.283 ± 0.086
4.798GlyThr: 4.798 ± 0.079
6.652GlyVal: 6.652 ± 0.073
1.574GlyTrp: 1.574 ± 0.038
2.167GlyTyr: 2.167 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
2.498HisAla: 2.498 ± 0.045
0.235HisCys: 0.235 ± 0.013
1.258HisAsp: 1.258 ± 0.029
0.95HisGlu: 0.95 ± 0.029
0.808HisPhe: 0.808 ± 0.026
1.901HisGly: 1.901 ± 0.042
0.562HisHis: 0.562 ± 0.024
0.924HisIle: 0.924 ± 0.025
0.434HisLys: 0.434 ± 0.018
2.289HisLeu: 2.289 ± 0.041
0.5HisMet: 0.5 ± 0.02
0.414HisAsn: 0.414 ± 0.02
1.379HisPro: 1.379 ± 0.036
0.591HisGln: 0.591 ± 0.024
1.458HisArg: 1.458 ± 0.033
0.898HisSer: 0.898 ± 0.029
0.75HisThr: 0.75 ± 0.026
1.472HisVal: 1.472 ± 0.037
0.384HisTrp: 0.384 ± 0.018
0.519HisTyr: 0.519 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
6.952IleAla: 6.952 ± 0.082
0.517IleCys: 0.517 ± 0.022
3.271IleAsp: 3.271 ± 0.055
3.077IleGlu: 3.077 ± 0.057
1.534IlePhe: 1.534 ± 0.045
4.827IleGly: 4.827 ± 0.077
0.902IleHis: 0.902 ± 0.026
2.024IleIle: 2.024 ± 0.048
1.168IleLys: 1.168 ± 0.035
4.595IleLeu: 4.595 ± 0.076
1.0IleMet: 1.0 ± 0.028
1.2IleAsn: 1.2 ± 0.038
2.258IlePro: 2.258 ± 0.043
0.996IleGln: 0.996 ± 0.028
3.249IleArg: 3.249 ± 0.049
2.687IleSer: 2.687 ± 0.048
2.902IleThr: 2.902 ± 0.057
3.499IleVal: 3.499 ± 0.061
0.725IleTrp: 0.725 ± 0.021
0.984IleTyr: 0.984 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
3.622LysAla: 3.622 ± 0.063
0.148LysCys: 0.148 ± 0.01
1.514LysAsp: 1.514 ± 0.037
1.23LysGlu: 1.23 ± 0.032
0.757LysPhe: 0.757 ± 0.027
2.604LysGly: 2.604 ± 0.058
0.51LysHis: 0.51 ± 0.021
1.4LysIle: 1.4 ± 0.044
0.892LysLys: 0.892 ± 0.034
2.586LysLeu: 2.586 ± 0.054
0.766LysMet: 0.766 ± 0.026
0.617LysAsn: 0.617 ± 0.023
1.688LysPro: 1.688 ± 0.043
0.763LysGln: 0.763 ± 0.026
1.931LysArg: 1.931 ± 0.048
1.571LysSer: 1.571 ± 0.039
1.673LysThr: 1.673 ± 0.045
2.061LysVal: 2.061 ± 0.045
0.337LysTrp: 0.337 ± 0.015
0.499LysTyr: 0.499 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
14.846LeuAla: 14.846 ± 0.146
1.035LeuCys: 1.035 ± 0.033
5.806LeuAsp: 5.806 ± 0.072
5.098LeuGlu: 5.098 ± 0.075
3.486LeuPhe: 3.486 ± 0.064
8.788LeuGly: 8.788 ± 0.095
2.051LeuHis: 2.051 ± 0.04
5.072LeuIle: 5.072 ± 0.078
2.758LeuLys: 2.758 ± 0.055
9.175LeuLeu: 9.175 ± 0.109
2.775LeuMet: 2.775 ± 0.051
2.531LeuAsn: 2.531 ± 0.043
6.234LeuPro: 6.234 ± 0.07
2.808LeuGln: 2.808 ± 0.046
8.014LeuArg: 8.014 ± 0.09
6.439LeuSer: 6.439 ± 0.069
6.286LeuThr: 6.286 ± 0.076
7.273LeuVal: 7.273 ± 0.097
1.451LeuTrp: 1.451 ± 0.035
1.867LeuTyr: 1.867 ± 0.038
0.0LeuXaa: 0.0 ± 0.0
Met
3.734MetAla: 3.734 ± 0.063
0.161MetCys: 0.161 ± 0.013
1.314MetAsp: 1.314 ± 0.035
1.252MetGlu: 1.252 ± 0.033
0.763MetPhe: 0.763 ± 0.026
2.214MetGly: 2.214 ± 0.039
0.454MetHis: 0.454 ± 0.019
1.427MetIle: 1.427 ± 0.036
0.896MetLys: 0.896 ± 0.028
2.588MetLeu: 2.588 ± 0.054
0.751MetMet: 0.751 ± 0.027
0.749MetAsn: 0.749 ± 0.022
1.531MetPro: 1.531 ± 0.037
1.004MetGln: 1.004 ± 0.03
1.915MetArg: 1.915 ± 0.041
1.474MetSer: 1.474 ± 0.037
2.101MetThr: 2.101 ± 0.037
1.93MetVal: 1.93 ± 0.044
0.23MetTrp: 0.23 ± 0.013
0.292MetTyr: 0.292 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.988AsnAla: 2.988 ± 0.052
0.21AsnCys: 0.21 ± 0.013
1.391AsnAsp: 1.391 ± 0.061
0.999AsnGlu: 0.999 ± 0.029
0.819AsnPhe: 0.819 ± 0.024
2.266AsnGly: 2.266 ± 0.049
0.469AsnHis: 0.469 ± 0.02
1.212AsnIle: 1.212 ± 0.036
0.568AsnLys: 0.568 ± 0.021
2.405AsnLeu: 2.405 ± 0.049
0.573AsnMet: 0.573 ± 0.021
0.591AsnAsn: 0.591 ± 0.025
1.864AsnPro: 1.864 ± 0.039
0.637AsnGln: 0.637 ± 0.024
1.736AsnArg: 1.736 ± 0.037
1.035AsnSer: 1.035 ± 0.033
1.244AsnThr: 1.244 ± 0.038
1.614AsnVal: 1.614 ± 0.038
0.431AsnTrp: 0.431 ± 0.019
0.523AsnTyr: 0.523 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
7.061ProAla: 7.061 ± 0.096
0.428ProCys: 0.428 ± 0.019
3.926ProAsp: 3.926 ± 0.059
4.025ProGlu: 4.025 ± 0.063
2.019ProPhe: 2.019 ± 0.041
4.797ProGly: 4.797 ± 0.059
1.105ProHis: 1.105 ± 0.029
2.038ProIle: 2.038 ± 0.041
1.488ProLys: 1.488 ± 0.036
5.08ProLeu: 5.08 ± 0.072
1.352ProMet: 1.352 ± 0.031
1.226ProAsn: 1.226 ± 0.032
2.685ProPro: 2.685 ± 0.059
1.884ProGln: 1.884 ± 0.039
3.16ProArg: 3.16 ± 0.054
2.491ProSer: 2.491 ± 0.047
2.405ProThr: 2.405 ± 0.046
4.632ProVal: 4.632 ± 0.073
0.713ProTrp: 0.713 ± 0.026
1.098ProTyr: 1.098 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
4.555GlnAla: 4.555 ± 0.065
0.189GlnCys: 0.189 ± 0.014
1.643GlnAsp: 1.643 ± 0.036
1.379GlnGlu: 1.379 ± 0.028
1.038GlnPhe: 1.038 ± 0.027
2.874GlnGly: 2.874 ± 0.046
0.618GlnHis: 0.618 ± 0.021
2.001GlnIle: 2.001 ± 0.037
0.946GlnLys: 0.946 ± 0.03
2.704GlnLeu: 2.704 ± 0.056
1.075GlnMet: 1.075 ± 0.028
0.839GlnAsn: 0.839 ± 0.026
1.748GlnPro: 1.748 ± 0.034
0.987GlnGln: 0.987 ± 0.033
2.406GlnArg: 2.406 ± 0.049
1.839GlnSer: 1.839 ± 0.038
1.937GlnThr: 1.937 ± 0.041
2.568GlnVal: 2.568 ± 0.04
0.404GlnTrp: 0.404 ± 0.016
0.496GlnTyr: 0.496 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
9.568ArgAla: 9.568 ± 0.097
0.498ArgCys: 0.498 ± 0.018
4.12ArgAsp: 4.12 ± 0.063
3.781ArgGlu: 3.781 ± 0.062
2.818ArgPhe: 2.818 ± 0.048
5.143ArgGly: 5.143 ± 0.066
1.688ArgHis: 1.688 ± 0.036
3.973ArgIle: 3.973 ± 0.056
2.112ArgLys: 2.112 ± 0.046
8.549ArgLeu: 8.549 ± 0.108
2.101ArgMet: 2.101 ± 0.038
1.736ArgAsn: 1.736 ± 0.041
3.778ArgPro: 3.778 ± 0.051
2.494ArgGln: 2.494 ± 0.055
5.487ArgArg: 5.487 ± 0.085
3.41ArgSer: 3.41 ± 0.056
3.054ArgThr: 3.054 ± 0.052
5.1ArgVal: 5.1 ± 0.064
1.013ArgTrp: 1.013 ± 0.029
1.511ArgTyr: 1.511 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
6.229SerAla: 6.229 ± 0.085
0.404SerCys: 0.404 ± 0.02
2.795SerAsp: 2.795 ± 0.048
2.519SerGlu: 2.519 ± 0.046
2.09SerPhe: 2.09 ± 0.034
5.49SerGly: 5.49 ± 0.083
0.978SerHis: 0.978 ± 0.027
2.216SerIle: 2.216 ± 0.053
1.214SerLys: 1.214 ± 0.034
5.144SerLeu: 5.144 ± 0.06
1.235SerMet: 1.235 ± 0.034
1.146SerAsn: 1.146 ± 0.032
2.613SerPro: 2.613 ± 0.047
1.509SerGln: 1.509 ± 0.036
3.438SerArg: 3.438 ± 0.051
2.408SerSer: 2.408 ± 0.059
2.456SerThr: 2.456 ± 0.052
3.838SerVal: 3.838 ± 0.069
0.699SerTrp: 0.699 ± 0.022
1.106SerTyr: 1.106 ± 0.025
0.0SerXaa: 0.0 ± 0.0
Thr
6.672ThrAla: 6.672 ± 0.088
0.462ThrCys: 0.462 ± 0.017
2.944ThrAsp: 2.944 ± 0.051
2.815ThrGlu: 2.815 ± 0.048
1.944ThrPhe: 1.944 ± 0.043
5.515ThrGly: 5.515 ± 0.09
1.055ThrHis: 1.055 ± 0.031
2.598ThrIle: 2.598 ± 0.059
1.318ThrLys: 1.318 ± 0.03
6.187ThrLeu: 6.187 ± 0.086
1.191ThrMet: 1.191 ± 0.035
1.229ThrAsn: 1.229 ± 0.04
3.485ThrPro: 3.485 ± 0.055
1.574ThrGln: 1.574 ± 0.037
3.519ThrArg: 3.519 ± 0.049
2.552ThrSer: 2.552 ± 0.048
2.89ThrThr: 2.89 ± 0.082
4.399ThrVal: 4.399 ± 0.09
0.71ThrTrp: 0.71 ± 0.022
1.115ThrTyr: 1.115 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
9.674ValAla: 9.674 ± 0.107
0.575ValCys: 0.575 ± 0.017
3.875ValAsp: 3.875 ± 0.056
4.298ValGlu: 4.298 ± 0.063
2.872ValPhe: 2.872 ± 0.05
5.358ValGly: 5.358 ± 0.072
1.277ValHis: 1.277 ± 0.031
4.129ValIle: 4.129 ± 0.067
2.024ValLys: 2.024 ± 0.05
7.887ValLeu: 7.887 ± 0.09
2.095ValMet: 2.095 ± 0.041
1.918ValAsn: 1.918 ± 0.049
3.839ValPro: 3.839 ± 0.06
2.389ValGln: 2.389 ± 0.034
4.499ValArg: 4.499 ± 0.053
4.015ValSer: 4.015 ± 0.07
4.95ValThr: 4.95 ± 0.08
5.88ValVal: 5.88 ± 0.086
1.142ValTrp: 1.142 ± 0.03
1.399ValTyr: 1.399 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.6TrpAla: 1.6 ± 0.036
0.142TrpCys: 0.142 ± 0.011
0.724TrpAsp: 0.724 ± 0.027
0.659TrpGlu: 0.659 ± 0.022
0.568TrpPhe: 0.568 ± 0.022
1.061TrpGly: 1.061 ± 0.03
0.367TrpHis: 0.367 ± 0.018
0.642TrpIle: 0.642 ± 0.025
0.397TrpLys: 0.397 ± 0.017
1.773TrpLeu: 1.773 ± 0.042
0.386TrpMet: 0.386 ± 0.015
0.413TrpAsn: 0.413 ± 0.017
0.773TrpPro: 0.773 ± 0.023
0.709TrpGln: 0.709 ± 0.024
1.261TrpArg: 1.261 ± 0.032
0.805TrpSer: 0.805 ± 0.025
0.845TrpThr: 0.845 ± 0.024
1.016TrpVal: 1.016 ± 0.027
0.242TrpTrp: 0.242 ± 0.015
0.285TrpTyr: 0.285 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.532TyrAla: 2.532 ± 0.043
0.21TyrCys: 0.21 ± 0.012
1.39TyrAsp: 1.39 ± 0.035
1.09TyrGlu: 1.09 ± 0.027
0.77TyrPhe: 0.77 ± 0.024
1.931TyrGly: 1.931 ± 0.04
0.465TyrHis: 0.465 ± 0.02
0.838TyrIle: 0.838 ± 0.026
0.471TyrLys: 0.471 ± 0.021
2.063TyrLeu: 2.063 ± 0.041
0.449TyrMet: 0.449 ± 0.022
0.492TyrAsn: 0.492 ± 0.017
1.019TyrPro: 1.019 ± 0.028
0.624TyrGln: 0.624 ± 0.021
1.515TyrArg: 1.515 ± 0.033
1.025TyrSer: 1.025 ± 0.029
1.018TyrThr: 1.018 ± 0.033
1.4TyrVal: 1.4 ± 0.031
0.332TyrTrp: 0.332 ± 0.016
0.471TyrTyr: 0.471 ± 0.018
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4261 proteins (1351082 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski