Amino acid dipepetide frequency for Flavobacterium phage 6H

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.531AlaAla: 2.531 ± 0.61
0.362AlaCys: 0.362 ± 0.146
2.748AlaAsp: 2.748 ± 0.481
4.05AlaGlu: 4.05 ± 0.814
2.965AlaPhe: 2.965 ± 0.431
3.472AlaGly: 3.472 ± 0.462
0.723AlaHis: 0.723 ± 0.241
5.135AlaIle: 5.135 ± 0.667
4.918AlaLys: 4.918 ± 0.918
5.425AlaLeu: 5.425 ± 0.861
1.013AlaMet: 1.013 ± 0.26
3.978AlaAsn: 3.978 ± 0.626
1.157AlaPro: 1.157 ± 0.301
2.314AlaGln: 2.314 ± 0.657
1.664AlaArg: 1.664 ± 0.275
4.123AlaSer: 4.123 ± 0.569
3.182AlaThr: 3.182 ± 0.589
2.821AlaVal: 2.821 ± 0.359
0.579AlaTrp: 0.579 ± 0.209
2.604AlaTyr: 2.604 ± 0.366
0.0AlaXaa: 0.0 ± 0.0
Cys
0.506CysAla: 0.506 ± 0.173
0.145CysCys: 0.145 ± 0.09
0.723CysAsp: 0.723 ± 0.249
0.362CysGlu: 0.362 ± 0.186
0.434CysPhe: 0.434 ± 0.154
0.217CysGly: 0.217 ± 0.117
0.145CysHis: 0.145 ± 0.103
0.145CysIle: 0.145 ± 0.089
0.94CysLys: 0.94 ± 0.305
0.579CysLeu: 0.579 ± 0.222
0.217CysMet: 0.217 ± 0.119
0.289CysAsn: 0.289 ± 0.187
0.579CysPro: 0.579 ± 0.218
0.289CysGln: 0.289 ± 0.153
0.289CysArg: 0.289 ± 0.173
0.434CysSer: 0.434 ± 0.204
0.434CysThr: 0.434 ± 0.137
0.289CysVal: 0.289 ± 0.144
0.072CysTrp: 0.072 ± 0.071
0.579CysTyr: 0.579 ± 0.219
0.0CysXaa: 0.0 ± 0.0
Asp
4.05AspAla: 4.05 ± 0.608
0.434AspCys: 0.434 ± 0.16
2.459AspAsp: 2.459 ± 0.541
3.978AspGlu: 3.978 ± 0.55
4.05AspPhe: 4.05 ± 0.516
3.182AspGly: 3.182 ± 0.461
0.506AspHis: 0.506 ± 0.231
4.701AspIle: 4.701 ± 0.622
5.786AspLys: 5.786 ± 0.516
4.846AspLeu: 4.846 ± 0.565
1.23AspMet: 1.23 ± 0.299
2.676AspAsn: 2.676 ± 0.525
1.013AspPro: 1.013 ± 0.262
1.447AspGln: 1.447 ± 0.358
2.025AspArg: 2.025 ± 0.348
3.255AspSer: 3.255 ± 0.683
2.459AspThr: 2.459 ± 0.567
3.399AspVal: 3.399 ± 0.45
1.085AspTrp: 1.085 ± 0.274
3.255AspTyr: 3.255 ± 0.517
0.0AspXaa: 0.0 ± 0.0
Glu
3.616GluAla: 3.616 ± 0.589
0.579GluCys: 0.579 ± 0.192
3.906GluAsp: 3.906 ± 0.483
5.425GluGlu: 5.425 ± 0.632
3.11GluPhe: 3.11 ± 0.379
3.978GluGly: 3.978 ± 0.512
1.519GluHis: 1.519 ± 0.275
6.654GluIle: 6.654 ± 0.831
6.871GluLys: 6.871 ± 0.644
7.739GluLeu: 7.739 ± 0.91
1.881GluMet: 1.881 ± 0.368
5.497GluAsn: 5.497 ± 0.523
1.085GluPro: 1.085 ± 0.279
2.604GluGln: 2.604 ± 0.405
3.038GluArg: 3.038 ± 0.518
3.978GluSer: 3.978 ± 0.44
3.038GluThr: 3.038 ± 0.485
4.484GluVal: 4.484 ± 0.603
0.94GluTrp: 0.94 ± 0.28
3.038GluTyr: 3.038 ± 0.508
0.0GluXaa: 0.0 ± 0.0
Phe
2.242PheAla: 2.242 ± 0.409
0.651PheCys: 0.651 ± 0.218
3.182PheAsp: 3.182 ± 0.432
4.267PheGlu: 4.267 ± 0.709
2.387PhePhe: 2.387 ± 0.58
2.893PheGly: 2.893 ± 0.582
0.796PheHis: 0.796 ± 0.257
3.978PheIle: 3.978 ± 0.585
3.978PheLys: 3.978 ± 0.46
4.34PheLeu: 4.34 ± 0.522
1.374PheMet: 1.374 ± 0.302
2.821PheAsn: 2.821 ± 0.533
1.519PhePro: 1.519 ± 0.304
1.953PheGln: 1.953 ± 0.343
2.459PheArg: 2.459 ± 0.419
4.123PheSer: 4.123 ± 0.534
2.821PheThr: 2.821 ± 0.361
2.459PheVal: 2.459 ± 0.466
1.013PheTrp: 1.013 ± 0.217
2.893PheTyr: 2.893 ± 0.526
0.0PheXaa: 0.0 ± 0.0
Gly
3.11GlyAla: 3.11 ± 0.7
0.434GlyCys: 0.434 ± 0.169
2.17GlyAsp: 2.17 ± 0.38
2.893GlyGlu: 2.893 ± 0.45
2.965GlyPhe: 2.965 ± 0.58
2.025GlyGly: 2.025 ± 0.423
0.723GlyHis: 0.723 ± 0.241
5.063GlyIle: 5.063 ± 0.628
4.267GlyLys: 4.267 ± 0.689
4.412GlyLeu: 4.412 ± 0.546
1.736GlyMet: 1.736 ± 0.365
3.978GlyAsn: 3.978 ± 0.51
0.651GlyPro: 0.651 ± 0.169
1.374GlyGln: 1.374 ± 0.364
1.953GlyArg: 1.953 ± 0.393
2.965GlySer: 2.965 ± 0.477
3.255GlyThr: 3.255 ± 0.344
2.893GlyVal: 2.893 ± 0.515
1.013GlyTrp: 1.013 ± 0.229
2.459GlyTyr: 2.459 ± 0.368
0.0GlyXaa: 0.0 ± 0.0
His
0.579HisAla: 0.579 ± 0.231
0.072HisCys: 0.072 ± 0.077
0.723HisAsp: 0.723 ± 0.199
0.868HisGlu: 0.868 ± 0.209
1.23HisPhe: 1.23 ± 0.349
1.013HisGly: 1.013 ± 0.248
0.289HisHis: 0.289 ± 0.15
1.374HisIle: 1.374 ± 0.27
1.664HisLys: 1.664 ± 0.444
2.097HisLeu: 2.097 ± 0.395
0.289HisMet: 0.289 ± 0.12
1.23HisAsn: 1.23 ± 0.252
0.362HisPro: 0.362 ± 0.142
0.434HisGln: 0.434 ± 0.161
0.796HisArg: 0.796 ± 0.208
0.94HisSer: 0.94 ± 0.244
0.868HisThr: 0.868 ± 0.208
0.434HisVal: 0.434 ± 0.167
0.072HisTrp: 0.072 ± 0.077
0.868HisTyr: 0.868 ± 0.173
0.0HisXaa: 0.0 ± 0.0
Ile
5.063IleAla: 5.063 ± 0.737
0.579IleCys: 0.579 ± 0.222
4.991IleAsp: 4.991 ± 0.532
8.535IleGlu: 8.535 ± 0.663
3.689IlePhe: 3.689 ± 0.469
3.472IleGly: 3.472 ± 0.54
2.17IleHis: 2.17 ± 0.336
5.859IleIle: 5.859 ± 0.691
9.113IleLys: 9.113 ± 0.736
6.799IleLeu: 6.799 ± 0.671
1.881IleMet: 1.881 ± 0.344
6.292IleAsn: 6.292 ± 0.868
3.11IlePro: 3.11 ± 0.41
3.327IleGln: 3.327 ± 0.499
3.255IleArg: 3.255 ± 0.546
6.076IleSer: 6.076 ± 0.614
4.484IleThr: 4.484 ± 0.608
4.557IleVal: 4.557 ± 0.511
0.579IleTrp: 0.579 ± 0.186
2.893IleTyr: 2.893 ± 0.429
0.0IleXaa: 0.0 ± 0.0
Lys
5.497LysAla: 5.497 ± 0.853
0.362LysCys: 0.362 ± 0.167
4.267LysAsp: 4.267 ± 0.421
7.45LysGlu: 7.45 ± 0.917
4.05LysPhe: 4.05 ± 0.481
3.472LysGly: 3.472 ± 0.438
1.447LysHis: 1.447 ± 0.279
9.258LysIle: 9.258 ± 0.803
8.896LysLys: 8.896 ± 1.052
7.088LysLeu: 7.088 ± 0.597
2.025LysMet: 2.025 ± 0.387
7.377LysAsn: 7.377 ± 0.551
2.748LysPro: 2.748 ± 0.437
3.255LysGln: 3.255 ± 0.623
3.906LysArg: 3.906 ± 0.502
6.799LysSer: 6.799 ± 0.606
5.28LysThr: 5.28 ± 0.623
5.859LysVal: 5.859 ± 0.551
1.013LysTrp: 1.013 ± 0.271
4.34LysTyr: 4.34 ± 0.557
0.0LysXaa: 0.0 ± 0.0
Leu
5.352LeuAla: 5.352 ± 0.609
0.651LeuCys: 0.651 ± 0.235
4.701LeuAsp: 4.701 ± 0.609
6.365LeuGlu: 6.365 ± 0.588
4.267LeuPhe: 4.267 ± 0.687
3.833LeuGly: 3.833 ± 0.548
1.23LeuHis: 1.23 ± 0.278
6.799LeuIle: 6.799 ± 0.934
8.318LeuLys: 8.318 ± 0.665
7.594LeuLeu: 7.594 ± 0.868
1.808LeuMet: 1.808 ± 0.335
6.654LeuAsn: 6.654 ± 0.806
4.34LeuPro: 4.34 ± 0.679
4.267LeuGln: 4.267 ± 0.546
3.761LeuArg: 3.761 ± 0.478
5.786LeuSer: 5.786 ± 0.5
4.412LeuThr: 4.412 ± 0.577
3.978LeuVal: 3.978 ± 0.413
0.94LeuTrp: 0.94 ± 0.263
2.893LeuTyr: 2.893 ± 0.479
0.0LeuXaa: 0.0 ± 0.0
Met
1.736MetAla: 1.736 ± 0.363
0.145MetCys: 0.145 ± 0.139
1.374MetAsp: 1.374 ± 0.268
1.013MetGlu: 1.013 ± 0.259
0.868MetPhe: 0.868 ± 0.26
0.796MetGly: 0.796 ± 0.316
0.289MetHis: 0.289 ± 0.159
1.013MetIle: 1.013 ± 0.254
2.821MetLys: 2.821 ± 0.422
2.459MetLeu: 2.459 ± 0.573
0.651MetMet: 0.651 ± 0.259
1.664MetAsn: 1.664 ± 0.301
0.796MetPro: 0.796 ± 0.225
1.085MetGln: 1.085 ± 0.263
0.796MetArg: 0.796 ± 0.197
1.881MetSer: 1.881 ± 0.27
1.519MetThr: 1.519 ± 0.278
1.013MetVal: 1.013 ± 0.228
0.289MetTrp: 0.289 ± 0.169
0.651MetTyr: 0.651 ± 0.216
0.0MetXaa: 0.0 ± 0.0
Asn
3.255AsnAla: 3.255 ± 0.536
0.362AsnCys: 0.362 ± 0.14
4.484AsnAsp: 4.484 ± 0.603
4.701AsnGlu: 4.701 ± 0.637
3.761AsnPhe: 3.761 ± 0.495
3.906AsnGly: 3.906 ± 0.503
0.94AsnHis: 0.94 ± 0.224
7.088AsnIle: 7.088 ± 0.887
6.365AsnLys: 6.365 ± 0.607
5.569AsnLeu: 5.569 ± 0.638
1.23AsnMet: 1.23 ± 0.246
4.774AsnAsn: 4.774 ± 0.581
2.242AsnPro: 2.242 ± 0.394
2.097AsnGln: 2.097 ± 0.409
3.327AsnArg: 3.327 ± 0.541
5.063AsnSer: 5.063 ± 0.5
3.906AsnThr: 3.906 ± 0.485
3.472AsnVal: 3.472 ± 0.494
0.868AsnTrp: 0.868 ± 0.299
2.676AsnTyr: 2.676 ± 0.575
0.0AsnXaa: 0.0 ± 0.0
Pro
1.447ProAla: 1.447 ± 0.324
0.362ProCys: 0.362 ± 0.148
1.374ProAsp: 1.374 ± 0.33
2.604ProGlu: 2.604 ± 0.394
1.447ProPhe: 1.447 ± 0.332
1.374ProGly: 1.374 ± 0.312
0.289ProHis: 0.289 ± 0.151
2.676ProIle: 2.676 ± 0.329
2.314ProLys: 2.314 ± 0.358
2.314ProLeu: 2.314 ± 0.426
0.796ProMet: 0.796 ± 0.289
2.242ProAsn: 2.242 ± 0.291
0.868ProPro: 0.868 ± 0.272
1.157ProGln: 1.157 ± 0.313
0.868ProArg: 0.868 ± 0.275
1.881ProSer: 1.881 ± 0.348
1.881ProThr: 1.881 ± 0.38
2.025ProVal: 2.025 ± 0.467
0.362ProTrp: 0.362 ± 0.152
1.23ProTyr: 1.23 ± 0.291
0.0ProXaa: 0.0 ± 0.0
Gln
2.17GlnAla: 2.17 ± 0.592
0.217GlnCys: 0.217 ± 0.129
1.664GlnAsp: 1.664 ± 0.331
3.544GlnGlu: 3.544 ± 0.451
1.302GlnPhe: 1.302 ± 0.338
1.519GlnGly: 1.519 ± 0.28
0.651GlnHis: 0.651 ± 0.179
3.906GlnIle: 3.906 ± 0.56
3.616GlnLys: 3.616 ± 0.459
3.689GlnLeu: 3.689 ± 0.414
0.94GlnMet: 0.94 ± 0.208
1.953GlnAsn: 1.953 ± 0.402
0.868GlnPro: 0.868 ± 0.211
1.591GlnGln: 1.591 ± 0.382
1.013GlnArg: 1.013 ± 0.269
1.519GlnSer: 1.519 ± 0.303
2.531GlnThr: 2.531 ± 0.363
1.736GlnVal: 1.736 ± 0.343
0.434GlnTrp: 0.434 ± 0.162
1.519GlnTyr: 1.519 ± 0.349
0.0GlnXaa: 0.0 ± 0.0
Arg
2.459ArgAla: 2.459 ± 0.332
0.651ArgCys: 0.651 ± 0.207
2.821ArgAsp: 2.821 ± 0.532
2.748ArgGlu: 2.748 ± 0.377
2.459ArgPhe: 2.459 ± 0.416
2.025ArgGly: 2.025 ± 0.31
0.723ArgHis: 0.723 ± 0.242
3.327ArgIle: 3.327 ± 0.46
3.616ArgLys: 3.616 ± 0.456
2.821ArgLeu: 2.821 ± 0.385
1.013ArgMet: 1.013 ± 0.305
2.314ArgAsn: 2.314 ± 0.35
0.723ArgPro: 0.723 ± 0.21
1.157ArgGln: 1.157 ± 0.258
1.085ArgArg: 1.085 ± 0.315
2.025ArgSer: 2.025 ± 0.44
1.591ArgThr: 1.591 ± 0.287
2.459ArgVal: 2.459 ± 0.297
0.217ArgTrp: 0.217 ± 0.15
0.94ArgTyr: 0.94 ± 0.257
0.0ArgXaa: 0.0 ± 0.0
Ser
3.761SerAla: 3.761 ± 0.452
0.579SerCys: 0.579 ± 0.199
4.34SerAsp: 4.34 ± 0.455
4.267SerGlu: 4.267 ± 0.573
4.557SerPhe: 4.557 ± 0.494
4.629SerGly: 4.629 ± 0.641
0.796SerHis: 0.796 ± 0.242
5.135SerIle: 5.135 ± 0.578
5.497SerLys: 5.497 ± 0.686
5.352SerLeu: 5.352 ± 0.534
1.519SerMet: 1.519 ± 0.396
4.05SerAsn: 4.05 ± 0.547
1.808SerPro: 1.808 ± 0.329
2.604SerGln: 2.604 ± 0.475
2.242SerArg: 2.242 ± 0.398
4.123SerSer: 4.123 ± 0.552
3.616SerThr: 3.616 ± 0.492
3.472SerVal: 3.472 ± 0.546
0.579SerTrp: 0.579 ± 0.188
2.025SerTyr: 2.025 ± 0.368
0.0SerXaa: 0.0 ± 0.0
Thr
3.11ThrAla: 3.11 ± 0.66
0.289ThrCys: 0.289 ± 0.146
3.327ThrAsp: 3.327 ± 0.509
3.761ThrGlu: 3.761 ± 0.577
3.11ThrPhe: 3.11 ± 0.426
3.689ThrGly: 3.689 ± 0.512
0.868ThrHis: 0.868 ± 0.223
5.135ThrIle: 5.135 ± 0.592
4.557ThrLys: 4.557 ± 0.528
4.701ThrLeu: 4.701 ± 0.49
0.723ThrMet: 0.723 ± 0.2
3.544ThrAsn: 3.544 ± 0.556
2.459ThrPro: 2.459 ± 0.439
1.374ThrGln: 1.374 ± 0.317
1.447ThrArg: 1.447 ± 0.362
2.676ThrSer: 2.676 ± 0.434
2.025ThrThr: 2.025 ± 0.324
2.604ThrVal: 2.604 ± 0.504
0.506ThrTrp: 0.506 ± 0.168
2.387ThrTyr: 2.387 ± 0.512
0.0ThrXaa: 0.0 ± 0.0
Val
3.182ValAla: 3.182 ± 0.586
0.506ValCys: 0.506 ± 0.181
3.544ValAsp: 3.544 ± 0.411
3.689ValGlu: 3.689 ± 0.423
2.531ValPhe: 2.531 ± 0.493
2.314ValGly: 2.314 ± 0.366
1.302ValHis: 1.302 ± 0.358
4.412ValIle: 4.412 ± 0.439
4.123ValLys: 4.123 ± 0.518
5.208ValLeu: 5.208 ± 0.558
1.23ValMet: 1.23 ± 0.258
4.34ValAsn: 4.34 ± 0.54
1.374ValPro: 1.374 ± 0.38
1.881ValGln: 1.881 ± 0.384
1.302ValArg: 1.302 ± 0.252
3.978ValSer: 3.978 ± 0.495
2.17ValThr: 2.17 ± 0.367
2.965ValVal: 2.965 ± 0.474
1.013ValTrp: 1.013 ± 0.346
2.025ValTyr: 2.025 ± 0.378
0.0ValXaa: 0.0 ± 0.0
Trp
0.579TrpAla: 0.579 ± 0.216
0.072TrpCys: 0.072 ± 0.065
0.796TrpAsp: 0.796 ± 0.239
0.506TrpGlu: 0.506 ± 0.188
0.434TrpPhe: 0.434 ± 0.144
0.579TrpGly: 0.579 ± 0.201
0.145TrpHis: 0.145 ± 0.101
1.157TrpIle: 1.157 ± 0.371
1.447TrpLys: 1.447 ± 0.316
1.591TrpLeu: 1.591 ± 0.382
0.362TrpMet: 0.362 ± 0.171
0.506TrpAsn: 0.506 ± 0.183
0.362TrpPro: 0.362 ± 0.203
0.506TrpGln: 0.506 ± 0.177
0.506TrpArg: 0.506 ± 0.214
0.579TrpSer: 0.579 ± 0.171
0.506TrpThr: 0.506 ± 0.194
0.796TrpVal: 0.796 ± 0.233
0.145TrpTrp: 0.145 ± 0.102
0.579TrpTyr: 0.579 ± 0.214
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.519TyrAla: 1.519 ± 0.377
0.217TyrCys: 0.217 ± 0.116
2.242TyrAsp: 2.242 ± 0.319
1.953TyrGlu: 1.953 ± 0.347
2.531TyrPhe: 2.531 ± 0.52
1.953TyrGly: 1.953 ± 0.423
0.651TyrHis: 0.651 ± 0.243
3.978TyrIle: 3.978 ± 0.527
5.28TyrLys: 5.28 ± 0.588
3.327TyrLeu: 3.327 ± 0.518
0.868TyrMet: 0.868 ± 0.218
3.906TyrAsn: 3.906 ± 0.654
1.447TyrPro: 1.447 ± 0.275
1.664TyrGln: 1.664 ± 0.32
1.447TyrArg: 1.447 ± 0.294
2.748TyrSer: 2.748 ± 0.423
2.314TyrThr: 2.314 ± 0.443
1.374TyrVal: 1.374 ± 0.347
0.434TyrTrp: 0.434 ± 0.15
1.664TyrTyr: 1.664 ± 0.379
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (13827 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski