Haloactinospora alba

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; Haloactinospora

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4398 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A543N7F1|A0A543N7F1_9ACTN ABC-2 type transport system permease protein OS=Haloactinospora alba OX=405555 GN=FHX37_4462 PE=3 SV=1
MM1 pKa = 7.84PAPAHH6 pKa = 5.4PRR8 pKa = 11.84GWLASIAVATAAVTTAALLHH28 pKa = 6.04TPAHH32 pKa = 6.62AEE34 pKa = 4.19QNSSPDD40 pKa = 3.71TQDD43 pKa = 3.26VPSGDD48 pKa = 2.74SWQPDD53 pKa = 3.52EE54 pKa = 5.23EE55 pKa = 4.4VDD57 pKa = 3.56RR58 pKa = 11.84DD59 pKa = 3.82PSGGEE64 pKa = 3.78PPEE67 pKa = 4.45EE68 pKa = 4.43DD69 pKa = 2.34WKK71 pKa = 11.11QPHH74 pKa = 6.0EE75 pKa = 4.47RR76 pKa = 11.84EE77 pKa = 4.14ADD79 pKa = 3.63QQDD82 pKa = 3.93GLSAQDD88 pKa = 3.4TDD90 pKa = 3.86IDD92 pKa = 5.08AEE94 pKa = 4.33DD95 pKa = 3.9SCDD98 pKa = 3.53DD99 pKa = 3.46GTNRR103 pKa = 11.84GVQDD107 pKa = 3.67HH108 pKa = 6.64YY109 pKa = 11.06PLQRR113 pKa = 11.84HH114 pKa = 5.33QISDD118 pKa = 3.65RR119 pKa = 11.84LEE121 pKa = 4.92LDD123 pKa = 3.55VNLEE127 pKa = 3.9NGNTVLRR134 pKa = 11.84HH135 pKa = 6.19RR136 pKa = 11.84EE137 pKa = 3.78LTIPGTGIDD146 pKa = 3.95LSLSSVYY153 pKa = 10.57NSQDD157 pKa = 2.99LGTNGWKK164 pKa = 10.69LNTGTDD170 pKa = 3.16VGLDD174 pKa = 4.36FITDD178 pKa = 3.29SDD180 pKa = 4.01DD181 pKa = 3.42VVFRR185 pKa = 11.84GPSGFCEE192 pKa = 4.24TFTDD196 pKa = 3.94QGDD199 pKa = 3.85GTFASPEE206 pKa = 3.95DD207 pKa = 3.67MEE209 pKa = 7.23ADD211 pKa = 4.97LEE213 pKa = 4.16EE214 pKa = 5.44LDD216 pKa = 3.5NGRR219 pKa = 11.84YY220 pKa = 9.84ALTFHH225 pKa = 6.39QGPYY229 pKa = 9.74ADD231 pKa = 4.02QMWTFTADD239 pKa = 2.53GWLYY243 pKa = 10.89AQSDD247 pKa = 4.14HH248 pKa = 6.83NGNTTTLNYY257 pKa = 10.64DD258 pKa = 3.52DD259 pKa = 6.09DD260 pKa = 4.81GLITSITDD268 pKa = 3.18DD269 pKa = 3.31QDD271 pKa = 3.44RR272 pKa = 11.84VTEE275 pKa = 4.45LDD277 pKa = 2.8WHH279 pKa = 5.34QNRR282 pKa = 11.84IGLTEE287 pKa = 3.83ITDD290 pKa = 3.78PTGATAADD298 pKa = 3.67YY299 pKa = 10.91SYY301 pKa = 11.63NADD304 pKa = 3.57GQLTEE309 pKa = 3.88ITDD312 pKa = 3.62RR313 pKa = 11.84AGNDD317 pKa = 2.65ITFAYY322 pKa = 10.25DD323 pKa = 3.58DD324 pKa = 4.37NNHH327 pKa = 6.36LSEE330 pKa = 3.98ITNARR335 pKa = 11.84GHH337 pKa = 5.57TWNLDD342 pKa = 3.01HH343 pKa = 7.51DD344 pKa = 4.42TAGQLTEE351 pKa = 4.74LDD353 pKa = 3.65QPVTGDD359 pKa = 3.11EE360 pKa = 4.33RR361 pKa = 11.84AVTTYY366 pKa = 11.44DD367 pKa = 2.9HH368 pKa = 7.07GEE370 pKa = 4.17AEE372 pKa = 4.59TEE374 pKa = 4.2VTDD377 pKa = 4.16PRR379 pKa = 11.84GNTSTHH385 pKa = 5.8TFDD388 pKa = 3.17EE389 pKa = 4.29QGRR392 pKa = 11.84QEE394 pKa = 4.28EE395 pKa = 4.43ATDD398 pKa = 3.76PEE400 pKa = 5.06GNTRR404 pKa = 11.84STTWTAAASVAAATDD419 pKa = 3.6PAGASTTYY427 pKa = 10.79DD428 pKa = 3.53YY429 pKa = 11.85DD430 pKa = 3.98DD431 pKa = 4.98LNNMVGTQLPSGAEE445 pKa = 3.97YY446 pKa = 10.66QIGYY450 pKa = 10.07ADD452 pKa = 3.43SANPHH457 pKa = 6.56KK458 pKa = 9.12PTSITAPEE466 pKa = 4.17EE467 pKa = 4.11EE468 pKa = 4.63VEE470 pKa = 4.84LDD472 pKa = 3.26YY473 pKa = 11.53DD474 pKa = 4.21DD475 pKa = 5.92RR476 pKa = 11.84GNLTQIRR483 pKa = 11.84RR484 pKa = 11.84PGAEE488 pKa = 3.95EE489 pKa = 4.18PEE491 pKa = 4.1QSLEE495 pKa = 3.95YY496 pKa = 10.89NNNGTLASQTNGQGAKK512 pKa = 9.39TEE514 pKa = 4.05FSYY517 pKa = 11.58DD518 pKa = 3.19EE519 pKa = 5.23DD520 pKa = 4.77GNLTEE525 pKa = 5.51IDD527 pKa = 3.69QPDD530 pKa = 3.94PLGTTSFTYY539 pKa = 10.41DD540 pKa = 2.85ALSRR544 pKa = 11.84VTSVTDD550 pKa = 3.76GNGTTLEE557 pKa = 4.05YY558 pKa = 10.38AYY560 pKa = 10.67DD561 pKa = 3.57KK562 pKa = 10.8LDD564 pKa = 3.45RR565 pKa = 11.84VVEE568 pKa = 4.24IVHH571 pKa = 6.98DD572 pKa = 3.96GDD574 pKa = 3.48IRR576 pKa = 11.84QSTEE580 pKa = 3.49YY581 pKa = 10.57NPNGDD586 pKa = 3.11VTATHH591 pKa = 6.97TPQATVAHH599 pKa = 7.47AYY601 pKa = 9.39NKK603 pKa = 10.12RR604 pKa = 11.84GEE606 pKa = 4.25VANTGRR612 pKa = 11.84DD613 pKa = 3.36DD614 pKa = 4.37GQDD617 pKa = 3.45HH618 pKa = 6.93EE619 pKa = 5.58SYY621 pKa = 10.96DD622 pKa = 3.63YY623 pKa = 11.14TYY625 pKa = 11.23DD626 pKa = 3.25QAGNLTSLSEE636 pKa = 4.23HH637 pKa = 6.8GATTDD642 pKa = 3.65YY643 pKa = 11.28AYY645 pKa = 9.19DD646 pKa = 3.46TANRR650 pKa = 11.84LTTVTDD656 pKa = 3.77DD657 pKa = 3.79SGGEE661 pKa = 3.92TTIGYY666 pKa = 10.04DD667 pKa = 3.11EE668 pKa = 4.95AGNRR672 pKa = 11.84DD673 pKa = 4.59EE674 pKa = 4.9ITFPDD679 pKa = 4.64GATQSLDD686 pKa = 3.4HH687 pKa = 6.85NEE689 pKa = 4.11AGLLTEE695 pKa = 4.85SVMANADD702 pKa = 3.39GDD704 pKa = 4.37TLAEE708 pKa = 3.93AAYY711 pKa = 10.4SYY713 pKa = 11.21TDD715 pKa = 5.2DD716 pKa = 5.79DD717 pKa = 4.68GQDD720 pKa = 3.31TTKK723 pKa = 10.79LQSRR727 pKa = 11.84TVNGQSRR734 pKa = 11.84EE735 pKa = 3.87FTYY738 pKa = 10.57DD739 pKa = 2.64EE740 pKa = 4.38RR741 pKa = 11.84GRR743 pKa = 11.84LTSDD747 pKa = 2.33GHH749 pKa = 5.72TDD751 pKa = 3.43YY752 pKa = 11.14TYY754 pKa = 11.45DD755 pKa = 3.55EE756 pKa = 4.88ADD758 pKa = 3.57NLTSAGDD765 pKa = 3.15TDD767 pKa = 4.15YY768 pKa = 11.44QVNKK772 pKa = 9.69ADD774 pKa = 3.65QVTEE778 pKa = 3.89VDD780 pKa = 3.97DD781 pKa = 4.11TEE783 pKa = 5.27LGYY786 pKa = 10.86DD787 pKa = 3.24QAGNLTEE794 pKa = 5.55AGQTDD799 pKa = 3.73IDD801 pKa = 4.2YY802 pKa = 11.0SPTGQMVDD810 pKa = 3.23KK811 pKa = 10.85DD812 pKa = 4.1AGNSASDD819 pKa = 3.64LQVSYY824 pKa = 8.96DD825 pKa = 3.83TADD828 pKa = 3.1STQRR832 pKa = 11.84RR833 pKa = 11.84TITQGSGDD841 pKa = 3.96NQTEE845 pKa = 4.1HH846 pKa = 6.65TLTNTALGISSIEE859 pKa = 3.81QDD861 pKa = 3.3DD862 pKa = 4.18GDD864 pKa = 3.58RR865 pKa = 11.84TRR867 pKa = 11.84YY868 pKa = 9.57VRR870 pKa = 11.84DD871 pKa = 3.05PDD873 pKa = 3.51GRR875 pKa = 11.84MLGMITGGQRR885 pKa = 11.84YY886 pKa = 8.11NAATDD891 pKa = 3.82AQNSTLALAEE901 pKa = 5.24DD902 pKa = 4.61GTEE905 pKa = 4.09STDD908 pKa = 3.26PDD910 pKa = 3.33VAYY913 pKa = 10.32DD914 pKa = 3.59YY915 pKa = 10.89TPYY918 pKa = 10.95GQTEE922 pKa = 4.16TATAGSGDD930 pKa = 3.49QAAQTNPFTYY940 pKa = 9.73TGAYY944 pKa = 9.58EE945 pKa = 4.62LDD947 pKa = 3.94NGDD950 pKa = 3.3KK951 pKa = 11.03ALGHH955 pKa = 6.92RR956 pKa = 11.84YY957 pKa = 9.65LSQRR961 pKa = 11.84THH963 pKa = 7.24RR964 pKa = 11.84FTQQDD969 pKa = 3.79PSWQEE974 pKa = 3.76DD975 pKa = 3.4NLYY978 pKa = 10.13TYY980 pKa = 10.99AEE982 pKa = 4.6CDD984 pKa = 4.49PINKK988 pKa = 8.51MDD990 pKa = 4.27RR991 pKa = 11.84NGLSACGWAIGDD1003 pKa = 5.02FITGYY1008 pKa = 10.01ISYY1011 pKa = 9.63HH1012 pKa = 5.04FAVYY1016 pKa = 9.82GVAALLAGTVAVAAAPIAIAAVSTGIAIYY1045 pKa = 10.78GMGRR1049 pKa = 11.84GIAGMYY1055 pKa = 9.42HH1056 pKa = 6.01SCC1058 pKa = 4.77

Molecular weight: 114.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A543N9X2|A0A543N9X2_9ACTN Uncharacterized protein OS=Haloactinospora alba OX=405555 GN=FHX37_3949 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.93RR4 pKa = 11.84TYY6 pKa = 10.0QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.4VHH17 pKa = 5.5GFRR20 pKa = 11.84KK21 pKa = 9.9RR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIISARR35 pKa = 11.84RR36 pKa = 11.84NKK38 pKa = 9.3GRR40 pKa = 11.84ARR42 pKa = 11.84LTVSHH47 pKa = 7.05

Molecular weight: 5.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4398

0

4398

1363651

30

4669

310.1

33.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.402 ± 0.059

0.805 ± 0.011

6.116 ± 0.04

6.65 ± 0.037

2.688 ± 0.024

9.152 ± 0.034

2.407 ± 0.019

3.124 ± 0.026

1.645 ± 0.025

9.918 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.887 ± 0.015

2.042 ± 0.022

5.944 ± 0.03

2.937 ± 0.025

8.168 ± 0.04

5.825 ± 0.027

6.27 ± 0.028

8.469 ± 0.041

1.5 ± 0.017

2.051 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski