Amino acid dipepetide frequency for Synechocystis sp. (strain PCC 6803 / Kazusa)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.925AlaAla: 6.925 ± 0.102	0.835AlaCys: 0.835 ± 0.028	4.298AlaAsp: 4.298 ± 0.079	5.422AlaGlu: 5.422 ± 0.081	2.833AlaPhe: 2.833 ± 0.052	6.09AlaGly: 6.09 ± 0.094	1.413AlaHis: 1.413 ± 0.038	7.278AlaIle: 7.278 ± 0.094	3.928AlaLys: 3.928 ± 0.075	9.488AlaLeu: 9.488 ± 0.118	2.129AlaMet: 2.129 ± 0.05	3.279AlaAsn: 3.279 ± 0.075	3.635AlaPro: 3.635 ± 0.068	4.824AlaGln: 4.824 ± 0.079	3.564AlaArg: 3.564 ± 0.065	4.465AlaSer: 4.465 ± 0.078	4.543AlaThr: 4.543 ± 0.087	5.706AlaVal: 5.706 ± 0.095	1.185AlaTrp: 1.185 ± 0.039	2.24AlaTyr: 2.24 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.648CysAla: 0.648 ± 0.022	0.162CysCys: 0.162 ± 0.012	0.523CysAsp: 0.523 ± 0.025	0.499CysGlu: 0.499 ± 0.024	0.441CysPhe: 0.441 ± 0.02	0.816CysGly: 0.816 ± 0.032	0.329CysHis: 0.329 ± 0.016	0.449CysIle: 0.449 ± 0.021	0.253CysLys: 0.253 ± 0.015	1.423CysLeu: 1.423 ± 0.043	0.15CysMet: 0.15 ± 0.013	0.314CysAsn: 0.314 ± 0.017	0.71CysPro: 0.71 ± 0.031	0.702CysGln: 0.702 ± 0.027	0.646CysArg: 0.646 ± 0.026	0.585CysSer: 0.585 ± 0.026	0.432CysThr: 0.432 ± 0.019	0.551CysVal: 0.551 ± 0.026	0.137CysTrp: 0.137 ± 0.011	0.373CysTyr: 0.373 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	3.229AspAla: 3.229 ± 0.056	0.605AspCys: 0.605 ± 0.026	2.407AspAsp: 2.407 ± 0.07	2.637AspGlu: 2.637 ± 0.058	2.489AspPhe: 2.489 ± 0.051	3.695AspGly: 3.695 ± 0.094	1.095AspHis: 1.095 ± 0.033	3.06AspIle: 3.06 ± 0.076	1.839AspLys: 1.839 ± 0.049	6.389AspLeu: 6.389 ± 0.081	0.801AspMet: 0.801 ± 0.026	2.106AspAsn: 2.106 ± 0.049	2.81AspPro: 2.81 ± 0.055	2.771AspGln: 2.771 ± 0.046	3.611AspArg: 3.611 ± 0.059	2.763AspSer: 2.763 ± 0.063	2.155AspThr: 2.155 ± 0.068	2.942AspVal: 2.942 ± 0.065	1.039AspTrp: 1.039 ± 0.033	2.072AspTyr: 2.072 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	5.533GluAla: 5.533 ± 0.078	0.436GluCys: 0.436 ± 0.02	2.879GluAsp: 2.879 ± 0.055	4.187GluGlu: 4.187 ± 0.08	2.271GluPhe: 2.271 ± 0.046	3.488GluGly: 3.488 ± 0.061	0.941GluHis: 0.941 ± 0.033	4.578GluIle: 4.578 ± 0.076	3.65GluLys: 3.65 ± 0.076	6.544GluLeu: 6.544 ± 0.086	1.321GluMet: 1.321 ± 0.039	2.715GluAsn: 2.715 ± 0.061	2.492GluPro: 2.492 ± 0.113	3.685GluGln: 3.685 ± 0.069	3.129GluArg: 3.129 ± 0.064	3.111GluSer: 3.111 ± 0.051	3.436GluThr: 3.436 ± 0.051	4.179GluVal: 4.179 ± 0.071	0.694GluTrp: 0.694 ± 0.027	1.579GluTyr: 1.579 ± 0.041	0.0GluXaa: 0.0 ± 0.0
Phe	3.285PheAla: 3.285 ± 0.059	0.589PheCys: 0.589 ± 0.026	2.358PheAsp: 2.358 ± 0.05	1.966PheGlu: 1.966 ± 0.041	1.869PhePhe: 1.869 ± 0.045	3.139PheGly: 3.139 ± 0.062	0.77PheHis: 0.77 ± 0.029	2.181PheIle: 2.181 ± 0.048	1.385PheLys: 1.385 ± 0.037	4.357PheLeu: 4.357 ± 0.079	0.715PheMet: 0.715 ± 0.026	1.773PheAsn: 1.773 ± 0.049	2.146PhePro: 2.146 ± 0.049	1.819PheGln: 1.819 ± 0.051	1.883PheArg: 1.883 ± 0.045	2.941PheSer: 2.941 ± 0.051	2.359PheThr: 2.359 ± 0.052	2.412PheVal: 2.412 ± 0.049	0.745PheTrp: 0.745 ± 0.029	1.381PheTyr: 1.381 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	5.253GlyAla: 5.253 ± 0.094	0.801GlyCys: 0.801 ± 0.029	4.387GlyAsp: 4.387 ± 0.096	4.738GlyGlu: 4.738 ± 0.074	3.161GlyPhe: 3.161 ± 0.062	5.428GlyGly: 5.428 ± 0.128	1.45GlyHis: 1.45 ± 0.046	5.072GlyIle: 5.072 ± 0.075	3.949GlyLys: 3.949 ± 0.064	7.944GlyLeu: 7.944 ± 0.113	1.645GlyMet: 1.645 ± 0.041	3.22GlyAsn: 3.22 ± 0.096	1.953GlyPro: 1.953 ± 0.042	4.049GlyGln: 4.049 ± 0.078	3.39GlyArg: 3.39 ± 0.062	4.091GlySer: 4.091 ± 0.083	3.968GlyThr: 3.968 ± 0.079	4.95GlyVal: 4.95 ± 0.071	1.206GlyTrp: 1.206 ± 0.035	2.356GlyTyr: 2.356 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	0.966HisAla: 0.966 ± 0.031	0.394HisCys: 0.394 ± 0.018	0.759HisAsp: 0.759 ± 0.032	0.743HisGlu: 0.743 ± 0.024	0.891HisPhe: 0.891 ± 0.031	1.512HisGly: 1.512 ± 0.041	0.717HisHis: 0.717 ± 0.03	0.968HisIle: 0.968 ± 0.031	0.577HisLys: 0.577 ± 0.025	2.334HisLeu: 2.334 ± 0.057	0.237HisMet: 0.237 ± 0.016	0.711HisAsn: 0.711 ± 0.025	1.368HisPro: 1.368 ± 0.038	1.352HisGln: 1.352 ± 0.037	1.287HisArg: 1.287 ± 0.038	1.147HisSer: 1.147 ± 0.028	0.829HisThr: 0.829 ± 0.027	0.738HisVal: 0.738 ± 0.024	0.531HisTrp: 0.531 ± 0.02	0.796HisTyr: 0.796 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	7.215IleAla: 7.215 ± 0.089	0.694IleCys: 0.694 ± 0.027	3.43IleAsp: 3.43 ± 0.064	3.618IleGlu: 3.618 ± 0.056	2.699IlePhe: 2.699 ± 0.057	4.218IleGly: 4.218 ± 0.078	1.154IleHis: 1.154 ± 0.035	3.693IleIle: 3.693 ± 0.073	2.66IleLys: 2.66 ± 0.066	6.479IleLeu: 6.479 ± 0.085	0.942IleMet: 0.942 ± 0.027	2.938IleAsn: 2.938 ± 0.067	3.452IlePro: 3.452 ± 0.063	2.524IleGln: 2.524 ± 0.05	2.91IleArg: 2.91 ± 0.058	3.8IleSer: 3.8 ± 0.067	3.692IleThr: 3.692 ± 0.067	4.057IleVal: 4.057 ± 0.07	0.841IleTrp: 0.841 ± 0.032	1.909IleTyr: 1.909 ± 0.042	0.0IleXaa: 0.0 ± 0.0
Lys	3.659LysAla: 3.659 ± 0.063	0.257LysCys: 0.257 ± 0.015	1.975LysAsp: 1.975 ± 0.046	2.79LysGlu: 2.79 ± 0.063	1.648LysPhe: 1.648 ± 0.042	2.474LysGly: 2.474 ± 0.051	0.667LysHis: 0.667 ± 0.024	3.092LysIle: 3.092 ± 0.065	2.242LysLys: 2.242 ± 0.063	4.819LysLeu: 4.819 ± 0.078	0.886LysMet: 0.886 ± 0.027	1.919LysAsn: 1.919 ± 0.046	2.074LysPro: 2.074 ± 0.041	2.34LysGln: 2.34 ± 0.048	2.16LysArg: 2.16 ± 0.051	2.377LysSer: 2.377 ± 0.046	2.696LysThr: 2.696 ± 0.05	3.017LysVal: 3.017 ± 0.052	0.407LysTrp: 0.407 ± 0.022	1.15LysTyr: 1.15 ± 0.037	0.0LysXaa: 0.0 ± 0.0
Leu	11.093LeuAla: 11.093 ± 0.126	1.047LeuCys: 1.047 ± 0.031	5.838LeuAsp: 5.838 ± 0.075	7.434LeuGlu: 7.434 ± 0.111	3.864LeuPhe: 3.864 ± 0.07	8.934LeuGly: 8.934 ± 0.129	1.805LeuHis: 1.805 ± 0.046	6.587LeuIle: 6.587 ± 0.096	5.064LeuLys: 5.064 ± 0.083	11.931LeuLeu: 11.931 ± 0.15	2.335LeuMet: 2.335 ± 0.044	4.211LeuAsn: 4.211 ± 0.064	6.317LeuPro: 6.317 ± 0.098	5.826LeuGln: 5.826 ± 0.096	5.522LeuArg: 5.522 ± 0.093	7.07LeuSer: 7.07 ± 0.087	7.163LeuThr: 7.163 ± 0.087	7.686LeuVal: 7.686 ± 0.1	1.613LeuTrp: 1.613 ± 0.051	2.635LeuTyr: 2.635 ± 0.05	0.001LeuXaa: 0.001 ± 0.001
Met	2.525MetAla: 2.525 ± 0.047	0.131MetCys: 0.131 ± 0.011	0.93MetAsp: 0.93 ± 0.031	1.217MetGlu: 1.217 ± 0.034	0.606MetPhe: 0.606 ± 0.024	1.812MetGly: 1.812 ± 0.043	0.273MetHis: 0.273 ± 0.016	1.01MetIle: 1.01 ± 0.031	0.854MetLys: 0.854 ± 0.026	1.855MetLeu: 1.855 ± 0.044	0.465MetMet: 0.465 ± 0.025	0.731MetAsn: 0.731 ± 0.028	1.019MetPro: 1.019 ± 0.031	0.742MetGln: 0.742 ± 0.026	0.881MetArg: 0.881 ± 0.033	1.058MetSer: 1.058 ± 0.027	1.374MetThr: 1.374 ± 0.033	1.882MetVal: 1.882 ± 0.04	0.146MetTrp: 0.146 ± 0.012	0.327MetTyr: 0.327 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.922AsnAla: 2.922 ± 0.067	0.47AsnCys: 0.47 ± 0.023	1.842AsnAsp: 1.842 ± 0.06	1.622AsnGlu: 1.622 ± 0.041	2.044AsnPhe: 2.044 ± 0.046	2.895AsnGly: 2.895 ± 0.098	0.851AsnHis: 0.851 ± 0.03	2.301AsnIle: 2.301 ± 0.055	1.325AsnLys: 1.325 ± 0.04	5.595AsnLeu: 5.595 ± 0.087	0.605AsnMet: 0.605 ± 0.025	1.861AsnAsn: 1.861 ± 0.056	2.945AsnPro: 2.945 ± 0.068	2.664AsnGln: 2.664 ± 0.065	2.172AsnArg: 2.172 ± 0.048	2.477AsnSer: 2.477 ± 0.063	1.888AsnThr: 1.888 ± 0.056	2.165AsnVal: 2.165 ± 0.053	0.69AsnTrp: 0.69 ± 0.031	1.439AsnTyr: 1.439 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	3.838ProAla: 3.838 ± 0.073	0.419ProCys: 0.419 ± 0.02	3.042ProAsp: 3.042 ± 0.052	3.977ProGlu: 3.977 ± 0.109	1.811ProPhe: 1.811 ± 0.046	3.619ProGly: 3.619 ± 0.076	1.079ProHis: 1.079 ± 0.037	2.937ProIle: 2.937 ± 0.051	1.951ProLys: 1.951 ± 0.046	5.678ProLeu: 5.678 ± 0.091	1.046ProMet: 1.046 ± 0.031	2.153ProAsn: 2.153 ± 0.056	2.789ProPro: 2.789 ± 0.075	2.897ProGln: 2.897 ± 0.051	2.117ProArg: 2.117 ± 0.05	3.143ProSer: 3.143 ± 0.071	2.906ProThr: 2.906 ± 0.063	3.493ProVal: 3.493 ± 0.058	0.88ProTrp: 0.88 ± 0.029	1.314ProTyr: 1.314 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	5.347GlnAla: 5.347 ± 0.09	0.477GlnCys: 0.477 ± 0.023	2.243GlnAsp: 2.243 ± 0.048	3.577GlnGlu: 3.577 ± 0.071	1.926GlnPhe: 1.926 ± 0.047	4.403GlnGly: 4.403 ± 0.084	0.801GlnHis: 0.801 ± 0.028	3.407GlnIle: 3.407 ± 0.061	2.514GlnLys: 2.514 ± 0.048	6.084GlnLeu: 6.084 ± 0.106	1.003GlnMet: 1.003 ± 0.03	2.076GlnAsn: 2.076 ± 0.049	2.579GlnPro: 2.579 ± 0.048	3.59GlnGln: 3.59 ± 0.076	3.261GlnArg: 3.261 ± 0.063	3.202GlnSer: 3.202 ± 0.057	3.196GlnThr: 3.196 ± 0.047	3.605GlnVal: 3.605 ± 0.061	1.106GlnTrp: 1.106 ± 0.039	1.321GlnTyr: 1.321 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	3.21ArgAla: 3.21 ± 0.071	0.502ArgCys: 0.502 ± 0.022	2.324ArgAsp: 2.324 ± 0.049	3.253ArgGlu: 3.253 ± 0.054	2.161ArgPhe: 2.161 ± 0.043	3.2ArgGly: 3.2 ± 0.061	1.177ArgHis: 1.177 ± 0.034	3.05ArgIle: 3.05 ± 0.056	1.93ArgLys: 1.93 ± 0.046	6.364ArgLeu: 6.364 ± 0.1	0.959ArgMet: 0.959 ± 0.031	1.922ArgAsn: 1.922 ± 0.048	2.369ArgPro: 2.369 ± 0.053	3.726ArgGln: 3.726 ± 0.069	3.346ArgArg: 3.346 ± 0.066	3.161ArgSer: 3.161 ± 0.064	2.224ArgThr: 2.224 ± 0.042	3.133ArgVal: 3.133 ± 0.062	0.968ArgTrp: 0.968 ± 0.034	1.772ArgTyr: 1.772 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	4.316SerAla: 4.316 ± 0.07	0.565SerCys: 0.565 ± 0.024	2.644SerAsp: 2.644 ± 0.057	2.895SerGlu: 2.895 ± 0.057	2.57SerPhe: 2.57 ± 0.05	4.412SerGly: 4.412 ± 0.079	1.298SerHis: 1.298 ± 0.03	3.157SerIle: 3.157 ± 0.056	2.113SerLys: 2.113 ± 0.045	7.662SerLeu: 7.662 ± 0.1	1.206SerMet: 1.206 ± 0.031	2.289SerAsn: 2.289 ± 0.068	4.107SerPro: 4.107 ± 0.064	3.348SerGln: 3.348 ± 0.058	2.865SerArg: 2.865 ± 0.054	3.769SerSer: 3.769 ± 0.088	3.124SerThr: 3.124 ± 0.059	3.623SerVal: 3.623 ± 0.071	0.885SerTrp: 0.885 ± 0.029	1.753SerTyr: 1.753 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	5.18ThrAla: 5.18 ± 0.084	0.467ThrCys: 0.467 ± 0.02	2.732ThrAsp: 2.732 ± 0.059	3.405ThrGlu: 3.405 ± 0.058	2.109ThrPhe: 2.109 ± 0.053	4.348ThrGly: 4.348 ± 0.087	0.93ThrHis: 0.93 ± 0.032	3.745ThrIle: 3.745 ± 0.066	2.093ThrLys: 2.093 ± 0.042	6.207ThrLeu: 6.207 ± 0.064	1.163ThrMet: 1.163 ± 0.033	2.072ThrAsn: 2.072 ± 0.061	3.329ThrPro: 3.329 ± 0.064	2.259ThrGln: 2.259 ± 0.044	2.052ThrArg: 2.052 ± 0.041	2.983ThrSer: 2.983 ± 0.06	3.116ThrThr: 3.116 ± 0.072	4.541ThrVal: 4.541 ± 0.095	0.75ThrTrp: 0.75 ± 0.027	1.539ThrTyr: 1.539 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	5.93ValAla: 5.93 ± 0.089	0.711ValCys: 0.711 ± 0.026	3.686ValAsp: 3.686 ± 0.073	4.376ValGlu: 4.376 ± 0.065	2.579ValPhe: 2.579 ± 0.052	4.913ValGly: 4.913 ± 0.079	1.011ValHis: 1.011 ± 0.034	4.195ValIle: 4.195 ± 0.071	2.95ValLys: 2.95 ± 0.052	6.826ValLeu: 6.826 ± 0.086	1.585ValMet: 1.585 ± 0.034	2.938ValAsn: 2.938 ± 0.056	2.909ValPro: 2.909 ± 0.052	2.88ValGln: 2.88 ± 0.055	3.064ValArg: 3.064 ± 0.056	3.871ValSer: 3.871 ± 0.073	4.087ValThr: 4.087 ± 0.086	5.049ValVal: 5.049 ± 0.075	0.857ValTrp: 0.857 ± 0.03	1.865ValTyr: 1.865 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	1.025TrpAla: 1.025 ± 0.034	0.164TrpCys: 0.164 ± 0.012	0.686TrpAsp: 0.686 ± 0.025	0.873TrpGlu: 0.873 ± 0.03	0.674TrpPhe: 0.674 ± 0.028	1.153TrpGly: 1.153 ± 0.039	0.403TrpHis: 0.403 ± 0.021	0.797TrpIle: 0.797 ± 0.031	0.46TrpLys: 0.46 ± 0.024	2.296TrpLeu: 2.296 ± 0.061	0.264TrpMet: 0.264 ± 0.016	0.541TrpAsn: 0.541 ± 0.026	0.496TrpPro: 0.496 ± 0.024	1.683TrpGln: 1.683 ± 0.049	0.924TrpArg: 0.924 ± 0.03	0.839TrpSer: 0.839 ± 0.029	0.606TrpThr: 0.606 ± 0.025	0.924TrpVal: 0.924 ± 0.03	0.293TrpTrp: 0.293 ± 0.019	0.419TrpTyr: 0.419 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.984TyrAla: 1.984 ± 0.047	0.416TyrCys: 0.416 ± 0.02	1.532TyrAsp: 1.532 ± 0.039	1.618TyrGlu: 1.618 ± 0.045	1.425TyrPhe: 1.425 ± 0.04	2.464TyrGly: 2.464 ± 0.044	0.692TyrHis: 0.692 ± 0.029	1.367TyrIle: 1.367 ± 0.039	0.914TyrLys: 0.914 ± 0.035	3.699TyrLeu: 3.699 ± 0.059	0.377TyrMet: 0.377 ± 0.019	1.004TyrAsn: 1.004 ± 0.03	1.593TyrPro: 1.593 ± 0.039	2.037TyrGln: 2.037 ± 0.046	1.968TyrArg: 1.968 ± 0.049	1.75TyrSer: 1.75 ± 0.039	1.223TyrThr: 1.223 ± 0.037	1.628TyrVal: 1.628 ± 0.045	0.549TyrTrp: 0.549 ± 0.024	1.084TyrTyr: 1.084 ± 0.031	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3508 proteins (1123132 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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