Streptococcus phage CHPC676

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Brussowvirus; unclassified Brussowvirus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F6V1|A0A3G8F6V1_9CAUD Choline binding protein A OS=Streptococcus phage CHPC676 OX=2365040 GN=CHPC676_0025 PE=4 SV=1
MM1 pKa = 7.11VNWVDD6 pKa = 4.52ADD8 pKa = 4.28GNDD11 pKa = 4.32LPDD14 pKa = 3.91GTDD17 pKa = 3.28QEE19 pKa = 5.22FKK21 pKa = 11.07AGMYY25 pKa = 10.2FSFAGDD31 pKa = 3.52EE32 pKa = 4.3VNITDD37 pKa = 3.61TGYY40 pKa = 9.31GGYY43 pKa = 10.32YY44 pKa = 9.25GGYY47 pKa = 8.85YY48 pKa = 9.03YY49 pKa = 10.88RR50 pKa = 11.84KK51 pKa = 9.85FEE53 pKa = 4.13FGQFGTVWLSCWNKK67 pKa = 10.66DD68 pKa = 3.89DD69 pKa = 5.14LVNYY73 pKa = 7.38YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F6W0|A0A3G8F6W0_9CAUD Portal (Connector) protein OS=Streptococcus phage CHPC676 OX=2365040 GN=CHPC676_0004 PE=4 SV=1
MM1 pKa = 7.56SKK3 pKa = 9.22TKK5 pKa = 10.5RR6 pKa = 11.84GVCANCHH13 pKa = 4.61TVFEE17 pKa = 4.52VSKK20 pKa = 9.76KK21 pKa = 7.91QRR23 pKa = 11.84YY24 pKa = 8.42KK25 pKa = 10.36IKK27 pKa = 9.76NGKK30 pKa = 8.84SVFCSQTCSLEE41 pKa = 4.28KK42 pKa = 10.59YY43 pKa = 9.48GKK45 pKa = 8.13TKK47 pKa = 9.25ITISEE52 pKa = 4.36IPCFRR57 pKa = 11.84CGKK60 pKa = 9.0MFTPTYY66 pKa = 9.91SQYY69 pKa = 11.19KK70 pKa = 7.81RR71 pKa = 11.84YY72 pKa = 9.99KK73 pKa = 8.85YY74 pKa = 10.7NDD76 pKa = 3.28YY77 pKa = 10.92VSNSFCSNEE86 pKa = 3.99CRR88 pKa = 11.84WKK90 pKa = 10.73KK91 pKa = 10.23EE92 pKa = 4.07YY93 pKa = 9.51PCKK96 pKa = 10.6YY97 pKa = 8.82HH98 pKa = 7.57DD99 pKa = 5.03DD100 pKa = 4.04YY101 pKa = 12.18VSVFVNGKK109 pKa = 9.91EE110 pKa = 3.7ILLDD114 pKa = 3.54FDD116 pKa = 4.4VFEE119 pKa = 5.59KK120 pKa = 9.61YY121 pKa = 9.3TNTIYY126 pKa = 10.8VRR128 pKa = 11.84NDD130 pKa = 3.13KK131 pKa = 10.5ISNYY135 pKa = 8.5HH136 pKa = 4.86SVYY139 pKa = 10.59VFEE142 pKa = 4.99KK143 pKa = 9.91GKK145 pKa = 10.6KK146 pKa = 7.7VLSRR150 pKa = 11.84IIMSVTDD157 pKa = 3.35KK158 pKa = 11.25NKK160 pKa = 10.42YY161 pKa = 8.48VDD163 pKa = 4.77HH164 pKa = 7.19INGNPLDD171 pKa = 3.85NRR173 pKa = 11.84RR174 pKa = 11.84SNLRR178 pKa = 11.84VVSHH182 pKa = 5.96QEE184 pKa = 3.05NMMNKK189 pKa = 8.25KK190 pKa = 8.41TYY192 pKa = 10.6KK193 pKa = 10.75NNTSKK198 pKa = 10.74IKK200 pKa = 10.53GVNLNKK206 pKa = 10.19KK207 pKa = 8.12GLWVARR213 pKa = 11.84IQVRR217 pKa = 11.84NQRR220 pKa = 11.84IFLGSSKK227 pKa = 10.56DD228 pKa = 3.26KK229 pKa = 11.18SVAEE233 pKa = 4.0RR234 pKa = 11.84LRR236 pKa = 11.84IEE238 pKa = 4.16AEE240 pKa = 3.57KK241 pKa = 10.62KK242 pKa = 10.51YY243 pKa = 10.45FGKK246 pKa = 10.57YY247 pKa = 8.25DD248 pKa = 3.53RR249 pKa = 11.84KK250 pKa = 10.2YY251 pKa = 11.25LKK253 pKa = 10.64

Molecular weight: 29.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12171

47

1779

229.6

25.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.485 ± 1.025

0.55 ± 0.128

6.277 ± 0.476

6.458 ± 0.679

3.927 ± 0.176

7.132 ± 0.608

1.306 ± 0.193

6.811 ± 0.396

8.167 ± 0.573

7.69 ± 0.312

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.473 ± 0.258

5.612 ± 0.337

2.785 ± 0.213

3.714 ± 0.239

4.231 ± 0.391

6.943 ± 0.658

6.483 ± 0.33

6.433 ± 0.211

1.38 ± 0.176

4.141 ± 0.454
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski