Amino acid dipepetide frequency for Microbacterium phage Teamocil

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.432AlaAla: 17.432 ± 1.794
0.819AlaCys: 0.819 ± 0.259
7.722AlaAsp: 7.722 ± 0.865
7.605AlaGlu: 7.605 ± 1.014
3.802AlaPhe: 3.802 ± 0.585
9.359AlaGly: 9.359 ± 0.842
2.106AlaHis: 2.106 ± 0.358
4.563AlaIle: 4.563 ± 0.56
3.451AlaLys: 3.451 ± 0.419
11.582AlaLeu: 11.582 ± 1.311
2.749AlaMet: 2.749 ± 0.375
2.398AlaAsn: 2.398 ± 0.483
6.376AlaPro: 6.376 ± 0.654
3.919AlaGln: 3.919 ± 0.547
7.78AlaArg: 7.78 ± 0.858
7.195AlaSer: 7.195 ± 0.679
8.891AlaThr: 8.891 ± 1.086
7.254AlaVal: 7.254 ± 0.633
2.749AlaTrp: 2.749 ± 0.391
2.106AlaTyr: 2.106 ± 0.357
0.0AlaXaa: 0.0 ± 0.0
Cys
0.936CysAla: 0.936 ± 0.231
0.234CysCys: 0.234 ± 0.132
1.17CysAsp: 1.17 ± 0.284
0.643CysGlu: 0.643 ± 0.192
0.175CysPhe: 0.175 ± 0.085
1.345CysGly: 1.345 ± 0.301
0.409CysHis: 0.409 ± 0.149
0.292CysIle: 0.292 ± 0.119
0.0CysLys: 0.0 ± 0.0
0.643CysLeu: 0.643 ± 0.162
0.117CysMet: 0.117 ± 0.086
0.234CysAsn: 0.234 ± 0.121
0.819CysPro: 0.819 ± 0.198
0.117CysGln: 0.117 ± 0.088
0.819CysArg: 0.819 ± 0.256
0.234CysSer: 0.234 ± 0.127
0.292CysThr: 0.292 ± 0.119
0.819CysVal: 0.819 ± 0.205
0.058CysTrp: 0.058 ± 0.061
0.117CysTyr: 0.117 ± 0.082
0.0CysXaa: 0.0 ± 0.0
Asp
8.424AspAla: 8.424 ± 0.812
0.819AspCys: 0.819 ± 0.257
4.855AspAsp: 4.855 ± 0.626
5.44AspGlu: 5.44 ± 0.743
1.696AspPhe: 1.696 ± 0.312
6.318AspGly: 6.318 ± 0.601
1.17AspHis: 1.17 ± 0.27
2.691AspIle: 2.691 ± 0.367
1.579AspLys: 1.579 ± 0.275
5.674AspLeu: 5.674 ± 0.552
1.989AspMet: 1.989 ± 0.293
1.638AspAsn: 1.638 ± 0.276
3.685AspPro: 3.685 ± 0.571
2.047AspGln: 2.047 ± 0.302
4.972AspArg: 4.972 ± 0.567
2.808AspSer: 2.808 ± 0.383
3.627AspThr: 3.627 ± 0.412
4.68AspVal: 4.68 ± 0.59
1.521AspTrp: 1.521 ± 0.292
1.17AspTyr: 1.17 ± 0.352
0.0AspXaa: 0.0 ± 0.0
Glu
7.78GluAla: 7.78 ± 0.921
0.526GluCys: 0.526 ± 0.188
4.036GluAsp: 4.036 ± 0.581
4.738GluGlu: 4.738 ± 0.64
1.755GluPhe: 1.755 ± 0.283
4.446GluGly: 4.446 ± 0.56
1.93GluHis: 1.93 ± 0.471
1.228GluIle: 1.228 ± 0.314
1.872GluLys: 1.872 ± 0.314
3.334GluLeu: 3.334 ± 0.476
1.228GluMet: 1.228 ± 0.248
1.638GluAsn: 1.638 ± 0.286
4.504GluPro: 4.504 ± 0.594
2.164GluGln: 2.164 ± 0.357
5.382GluArg: 5.382 ± 0.629
4.095GluSer: 4.095 ± 0.566
3.744GluThr: 3.744 ± 0.516
5.674GluVal: 5.674 ± 0.741
1.345GluTrp: 1.345 ± 0.266
1.638GluTyr: 1.638 ± 0.346
0.0GluXaa: 0.0 ± 0.0
Phe
2.223PheAla: 2.223 ± 0.388
0.351PheCys: 0.351 ± 0.182
2.34PheAsp: 2.34 ± 0.358
2.106PheGlu: 2.106 ± 0.346
0.643PhePhe: 0.643 ± 0.208
2.164PheGly: 2.164 ± 0.437
0.292PheHis: 0.292 ± 0.119
1.17PheIle: 1.17 ± 0.279
0.585PheLys: 0.585 ± 0.171
1.579PheLeu: 1.579 ± 0.316
0.585PheMet: 0.585 ± 0.203
0.585PheAsn: 0.585 ± 0.199
1.053PhePro: 1.053 ± 0.272
0.643PheGln: 0.643 ± 0.187
1.579PheArg: 1.579 ± 0.279
1.228PheSer: 1.228 ± 0.281
2.34PheThr: 2.34 ± 0.345
2.223PheVal: 2.223 ± 0.398
0.468PheTrp: 0.468 ± 0.199
0.702PheTyr: 0.702 ± 0.171
0.0PheXaa: 0.0 ± 0.0
Gly
7.722GlyAla: 7.722 ± 0.82
0.877GlyCys: 0.877 ± 0.276
6.201GlyAsp: 6.201 ± 0.621
4.563GlyGlu: 4.563 ± 0.438
2.398GlyPhe: 2.398 ± 0.318
9.125GlyGly: 9.125 ± 0.97
2.281GlyHis: 2.281 ± 0.367
3.919GlyIle: 3.919 ± 0.452
2.457GlyLys: 2.457 ± 0.414
7.312GlyLeu: 7.312 ± 0.767
3.042GlyMet: 3.042 ± 0.44
2.398GlyAsn: 2.398 ± 0.44
3.861GlyPro: 3.861 ± 0.396
2.808GlyGln: 2.808 ± 0.544
5.557GlyArg: 5.557 ± 0.614
5.733GlySer: 5.733 ± 0.761
6.552GlyThr: 6.552 ± 0.922
6.142GlyVal: 6.142 ± 0.549
2.574GlyTrp: 2.574 ± 0.421
2.515GlyTyr: 2.515 ± 0.368
0.0GlyXaa: 0.0 ± 0.0
His
1.755HisAla: 1.755 ± 0.324
0.175HisCys: 0.175 ± 0.091
1.17HisAsp: 1.17 ± 0.248
1.404HisGlu: 1.404 ± 0.293
0.643HisPhe: 0.643 ± 0.153
1.813HisGly: 1.813 ± 0.385
0.526HisHis: 0.526 ± 0.198
1.053HisIle: 1.053 ± 0.245
0.351HisLys: 0.351 ± 0.136
1.755HisLeu: 1.755 ± 0.349
0.409HisMet: 0.409 ± 0.151
0.292HisAsn: 0.292 ± 0.136
1.755HisPro: 1.755 ± 0.351
0.234HisGln: 0.234 ± 0.134
1.404HisArg: 1.404 ± 0.301
1.404HisSer: 1.404 ± 0.286
1.053HisThr: 1.053 ± 0.251
0.994HisVal: 0.994 ± 0.282
0.526HisTrp: 0.526 ± 0.183
0.585HisTyr: 0.585 ± 0.182
0.0HisXaa: 0.0 ± 0.0
Ile
4.855IleAla: 4.855 ± 0.577
0.234IleCys: 0.234 ± 0.124
3.51IleAsp: 3.51 ± 0.414
3.802IleGlu: 3.802 ± 0.485
0.292IlePhe: 0.292 ± 0.126
3.802IleGly: 3.802 ± 0.399
0.994IleHis: 0.994 ± 0.239
2.164IleIle: 2.164 ± 0.432
0.819IleLys: 0.819 ± 0.198
2.457IleLeu: 2.457 ± 0.333
0.702IleMet: 0.702 ± 0.201
0.994IleAsn: 0.994 ± 0.297
2.457IlePro: 2.457 ± 0.47
1.462IleGln: 1.462 ± 0.334
2.457IleArg: 2.457 ± 0.43
2.34IleSer: 2.34 ± 0.451
3.978IleThr: 3.978 ± 0.425
4.212IleVal: 4.212 ± 0.47
0.702IleTrp: 0.702 ± 0.202
0.994IleTyr: 0.994 ± 0.266
0.0IleXaa: 0.0 ± 0.0
Lys
3.744LysAla: 3.744 ± 0.545
0.585LysCys: 0.585 ± 0.178
1.287LysAsp: 1.287 ± 0.329
0.643LysGlu: 0.643 ± 0.202
0.585LysPhe: 0.585 ± 0.206
2.34LysGly: 2.34 ± 0.348
0.585LysHis: 0.585 ± 0.193
0.877LysIle: 0.877 ± 0.229
0.409LysLys: 0.409 ± 0.194
0.936LysLeu: 0.936 ± 0.266
0.351LysMet: 0.351 ± 0.121
0.643LysAsn: 0.643 ± 0.169
2.047LysPro: 2.047 ± 0.361
0.702LysGln: 0.702 ± 0.222
2.515LysArg: 2.515 ± 0.463
1.638LysSer: 1.638 ± 0.289
0.877LysThr: 0.877 ± 0.276
2.866LysVal: 2.866 ± 0.423
0.292LysTrp: 0.292 ± 0.127
0.819LysTyr: 0.819 ± 0.234
0.0LysXaa: 0.0 ± 0.0
Leu
9.886LeuAla: 9.886 ± 0.893
0.702LeuCys: 0.702 ± 0.2
5.323LeuAsp: 5.323 ± 0.622
4.036LeuGlu: 4.036 ± 0.538
1.462LeuPhe: 1.462 ± 0.324
7.195LeuGly: 7.195 ± 1.005
0.936LeuHis: 0.936 ± 0.24
4.387LeuIle: 4.387 ± 0.591
0.643LeuLys: 0.643 ± 0.153
6.259LeuLeu: 6.259 ± 0.792
1.521LeuMet: 1.521 ± 0.24
1.696LeuAsn: 1.696 ± 0.317
5.031LeuPro: 5.031 ± 0.442
1.521LeuGln: 1.521 ± 0.244
4.972LeuArg: 4.972 ± 0.754
5.44LeuSer: 5.44 ± 0.752
7.02LeuThr: 7.02 ± 0.732
6.201LeuVal: 6.201 ± 0.627
1.053LeuTrp: 1.053 ± 0.23
2.047LeuTyr: 2.047 ± 0.375
0.0LeuXaa: 0.0 ± 0.0
Met
2.691MetAla: 2.691 ± 0.403
0.175MetCys: 0.175 ± 0.113
1.462MetAsp: 1.462 ± 0.318
1.053MetGlu: 1.053 ± 0.223
0.468MetPhe: 0.468 ± 0.171
1.93MetGly: 1.93 ± 0.324
0.292MetHis: 0.292 ± 0.119
1.579MetIle: 1.579 ± 0.302
0.585MetLys: 0.585 ± 0.184
1.755MetLeu: 1.755 ± 0.294
0.468MetMet: 0.468 ± 0.183
0.877MetAsn: 0.877 ± 0.227
1.228MetPro: 1.228 ± 0.245
0.643MetGln: 0.643 ± 0.212
1.696MetArg: 1.696 ± 0.269
2.632MetSer: 2.632 ± 0.371
2.398MetThr: 2.398 ± 0.4
1.17MetVal: 1.17 ± 0.268
0.351MetTrp: 0.351 ± 0.118
0.643MetTyr: 0.643 ± 0.191
0.0MetXaa: 0.0 ± 0.0
Asn
3.919AsnAla: 3.919 ± 0.399
0.292AsnCys: 0.292 ± 0.131
1.462AsnAsp: 1.462 ± 0.277
0.936AsnGlu: 0.936 ± 0.275
0.351AsnPhe: 0.351 ± 0.13
3.159AsnGly: 3.159 ± 0.513
0.409AsnHis: 0.409 ± 0.171
1.053AsnIle: 1.053 ± 0.277
0.175AsnLys: 0.175 ± 0.096
2.632AsnLeu: 2.632 ± 0.366
0.468AsnMet: 0.468 ± 0.167
0.468AsnAsn: 0.468 ± 0.173
1.462AsnPro: 1.462 ± 0.425
0.643AsnGln: 0.643 ± 0.208
2.047AsnArg: 2.047 ± 0.31
1.579AsnSer: 1.579 ± 0.268
1.813AsnThr: 1.813 ± 0.302
1.755AsnVal: 1.755 ± 0.379
0.468AsnTrp: 0.468 ± 0.148
0.76AsnTyr: 0.76 ± 0.201
0.0AsnXaa: 0.0 ± 0.0
Pro
6.201ProAla: 6.201 ± 0.675
0.526ProCys: 0.526 ± 0.193
4.153ProAsp: 4.153 ± 0.609
4.972ProGlu: 4.972 ± 0.584
1.579ProPhe: 1.579 ± 0.273
5.206ProGly: 5.206 ± 0.543
1.345ProHis: 1.345 ± 0.289
2.106ProIle: 2.106 ± 0.336
1.989ProLys: 1.989 ± 0.389
3.919ProLeu: 3.919 ± 0.403
1.345ProMet: 1.345 ± 0.281
1.989ProAsn: 1.989 ± 0.435
3.861ProPro: 3.861 ± 0.574
2.047ProGln: 2.047 ± 0.442
3.393ProArg: 3.393 ± 0.45
3.802ProSer: 3.802 ± 0.544
3.685ProThr: 3.685 ± 0.47
5.265ProVal: 5.265 ± 0.678
0.936ProTrp: 0.936 ± 0.275
1.521ProTyr: 1.521 ± 0.297
0.0ProXaa: 0.0 ± 0.0
Gln
4.27GlnAla: 4.27 ± 0.625
0.117GlnCys: 0.117 ± 0.083
1.287GlnAsp: 1.287 ± 0.262
1.345GlnGlu: 1.345 ± 0.278
1.17GlnPhe: 1.17 ± 0.268
2.164GlnGly: 2.164 ± 0.371
0.994GlnHis: 0.994 ± 0.231
1.755GlnIle: 1.755 ± 0.362
0.76GlnLys: 0.76 ± 0.22
1.228GlnLeu: 1.228 ± 0.351
0.994GlnMet: 0.994 ± 0.265
1.111GlnAsn: 1.111 ± 0.321
2.047GlnPro: 2.047 ± 0.306
0.994GlnGln: 0.994 ± 0.197
2.398GlnArg: 2.398 ± 0.416
1.579GlnSer: 1.579 ± 0.384
1.989GlnThr: 1.989 ± 0.322
2.164GlnVal: 2.164 ± 0.413
0.819GlnTrp: 0.819 ± 0.241
0.76GlnTyr: 0.76 ± 0.203
0.0GlnXaa: 0.0 ± 0.0
Arg
7.839ArgAla: 7.839 ± 0.581
0.76ArgCys: 0.76 ± 0.179
4.563ArgAsp: 4.563 ± 0.593
4.621ArgGlu: 4.621 ± 0.635
1.989ArgPhe: 1.989 ± 0.388
5.206ArgGly: 5.206 ± 0.557
1.17ArgHis: 1.17 ± 0.273
2.866ArgIle: 2.866 ± 0.467
2.223ArgLys: 2.223 ± 0.348
5.908ArgLeu: 5.908 ± 0.61
1.989ArgMet: 1.989 ± 0.413
1.872ArgAsn: 1.872 ± 0.417
3.627ArgPro: 3.627 ± 0.555
2.164ArgGln: 2.164 ± 0.324
6.844ArgArg: 6.844 ± 0.917
3.568ArgSer: 3.568 ± 0.51
4.036ArgThr: 4.036 ± 0.468
4.914ArgVal: 4.914 ± 0.536
1.872ArgTrp: 1.872 ± 0.337
2.398ArgTyr: 2.398 ± 0.408
0.0ArgXaa: 0.0 ± 0.0
Ser
6.844SerAla: 6.844 ± 0.852
0.234SerCys: 0.234 ± 0.117
4.504SerAsp: 4.504 ± 0.472
3.978SerGlu: 3.978 ± 0.522
1.404SerPhe: 1.404 ± 0.329
5.206SerGly: 5.206 ± 0.633
0.877SerHis: 0.877 ± 0.231
3.159SerIle: 3.159 ± 0.469
1.521SerLys: 1.521 ± 0.319
4.914SerLeu: 4.914 ± 0.534
1.872SerMet: 1.872 ± 0.324
2.047SerAsn: 2.047 ± 0.372
3.568SerPro: 3.568 ± 0.402
1.462SerGln: 1.462 ± 0.308
3.51SerArg: 3.51 ± 0.433
3.042SerSer: 3.042 ± 0.517
5.148SerThr: 5.148 ± 0.615
4.387SerVal: 4.387 ± 0.466
0.819SerTrp: 0.819 ± 0.255
1.696SerTyr: 1.696 ± 0.318
0.0SerXaa: 0.0 ± 0.0
Thr
9.652ThrAla: 9.652 ± 1.12
0.585ThrCys: 0.585 ± 0.175
4.504ThrAsp: 4.504 ± 0.519
3.51ThrGlu: 3.51 ± 0.542
1.696ThrPhe: 1.696 ± 0.321
7.429ThrGly: 7.429 ± 1.039
0.994ThrHis: 0.994 ± 0.27
3.217ThrIle: 3.217 ± 0.372
1.872ThrLys: 1.872 ± 0.333
6.142ThrLeu: 6.142 ± 0.588
1.17ThrMet: 1.17 ± 0.245
1.755ThrAsn: 1.755 ± 0.312
5.382ThrPro: 5.382 ± 0.721
2.223ThrGln: 2.223 ± 0.36
4.27ThrArg: 4.27 ± 0.564
3.685ThrSer: 3.685 ± 0.493
4.914ThrThr: 4.914 ± 0.736
5.967ThrVal: 5.967 ± 0.748
1.17ThrTrp: 1.17 ± 0.272
1.638ThrTyr: 1.638 ± 0.28
0.0ThrXaa: 0.0 ± 0.0
Val
9.067ValAla: 9.067 ± 0.664
0.702ValCys: 0.702 ± 0.189
3.627ValAsp: 3.627 ± 0.51
4.738ValGlu: 4.738 ± 0.497
1.579ValPhe: 1.579 ± 0.244
5.85ValGly: 5.85 ± 0.606
1.404ValHis: 1.404 ± 0.317
3.802ValIle: 3.802 ± 0.47
2.398ValLys: 2.398 ± 0.428
5.674ValLeu: 5.674 ± 0.451
1.989ValMet: 1.989 ± 0.319
2.223ValAsn: 2.223 ± 0.341
5.031ValPro: 5.031 ± 0.484
2.457ValGln: 2.457 ± 0.399
5.148ValArg: 5.148 ± 0.494
5.206ValSer: 5.206 ± 0.598
6.435ValThr: 6.435 ± 0.561
5.499ValVal: 5.499 ± 0.638
1.696ValTrp: 1.696 ± 0.319
1.111ValTyr: 1.111 ± 0.207
0.0ValXaa: 0.0 ± 0.0
Trp
2.047TrpAla: 2.047 ± 0.337
0.468TrpCys: 0.468 ± 0.164
1.228TrpAsp: 1.228 ± 0.278
1.521TrpGlu: 1.521 ± 0.314
0.76TrpPhe: 0.76 ± 0.229
1.462TrpGly: 1.462 ± 0.275
0.468TrpHis: 0.468 ± 0.168
0.76TrpIle: 0.76 ± 0.224
0.643TrpLys: 0.643 ± 0.163
1.755TrpLeu: 1.755 ± 0.366
0.175TrpMet: 0.175 ± 0.108
0.585TrpAsn: 0.585 ± 0.164
0.76TrpPro: 0.76 ± 0.223
0.819TrpGln: 0.819 ± 0.185
1.345TrpArg: 1.345 ± 0.275
1.696TrpSer: 1.696 ± 0.388
1.287TrpThr: 1.287 ± 0.298
1.345TrpVal: 1.345 ± 0.248
0.877TrpTrp: 0.877 ± 0.259
0.819TrpTyr: 0.819 ± 0.214
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.574TyrAla: 2.574 ± 0.316
0.292TyrCys: 0.292 ± 0.133
2.398TyrAsp: 2.398 ± 0.37
1.404TyrGlu: 1.404 ± 0.26
0.409TyrPhe: 0.409 ± 0.16
2.515TyrGly: 2.515 ± 0.399
0.117TyrHis: 0.117 ± 0.081
0.585TyrIle: 0.585 ± 0.146
0.409TyrLys: 0.409 ± 0.162
1.872TyrLeu: 1.872 ± 0.343
0.877TyrMet: 0.877 ± 0.246
0.409TyrAsn: 0.409 ± 0.129
1.287TyrPro: 1.287 ± 0.249
0.877TyrGln: 0.877 ± 0.228
2.34TyrArg: 2.34 ± 0.352
1.287TyrSer: 1.287 ± 0.263
1.696TyrThr: 1.696 ± 0.269
2.164TyrVal: 2.164 ± 0.351
0.526TyrTrp: 0.526 ± 0.188
0.643TyrTyr: 0.643 ± 0.202
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (17096 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski