Botrimarina mediterranea

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales;

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4587 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A518KA73|A0A518KA73_9BACT Anhydro-N-acetylmuramic acid kinase OS=Botrimarina mediterranea OX=2528022 GN=anmK_2 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.03FVQHH6 pKa = 6.46AASVAACLAAAVSTPLSVTAQQLSGDD32 pKa = 4.15YY33 pKa = 11.34LMDD36 pKa = 4.91DD37 pKa = 3.89ADD39 pKa = 3.67IAFLGTAYY47 pKa = 10.61LGRR50 pKa = 11.84VGFSVSSAGDD60 pKa = 3.5FNGDD64 pKa = 3.39GFDD67 pKa = 4.96DD68 pKa = 4.47FLFSAPNAPPNSQFSGEE85 pKa = 4.18TFLLYY90 pKa = 10.77GGAEE94 pKa = 4.1TGNGQRR100 pKa = 11.84TLSPMDD106 pKa = 4.15ADD108 pKa = 3.62VHH110 pKa = 5.46FVGIEE115 pKa = 4.06GQHH118 pKa = 5.39AGYY121 pKa = 9.7RR122 pKa = 11.84VSSAGDD128 pKa = 3.61LNLDD132 pKa = 3.88GLDD135 pKa = 4.23DD136 pKa = 4.71LLIATADD143 pKa = 3.54FSEE146 pKa = 4.38RR147 pKa = 11.84NKK149 pKa = 10.37VYY151 pKa = 10.47LVYY154 pKa = 10.69GRR156 pKa = 11.84PDD158 pKa = 3.29ANQLTGEE165 pKa = 4.28FQLSDD170 pKa = 3.24SDD172 pKa = 3.61ATFVLEE178 pKa = 4.33RR179 pKa = 11.84SSDD182 pKa = 3.66PGPVGLTPAGDD193 pKa = 3.51IDD195 pKa = 4.53GDD197 pKa = 4.11GAADD201 pKa = 4.57LLIGDD206 pKa = 3.98YY207 pKa = 10.05TYY209 pKa = 11.56GSGYY213 pKa = 9.5PGRR216 pKa = 11.84AYY218 pKa = 10.86LFYY221 pKa = 10.8GNPDD225 pKa = 3.14ASLSGGIDD233 pKa = 3.06IASADD238 pKa = 3.42ATFDD242 pKa = 3.1VDD244 pKa = 5.75FRR246 pKa = 11.84DD247 pKa = 3.65GTLGYY252 pKa = 9.58SVSSAGDD259 pKa = 3.63LNGDD263 pKa = 3.8GLDD266 pKa = 4.0DD267 pKa = 3.85VAIGALGQGEE277 pKa = 4.6VYY279 pKa = 10.4LYY281 pKa = 10.36YY282 pKa = 10.83GRR284 pKa = 11.84PTSEE288 pKa = 4.32RR289 pKa = 11.84IAGRR293 pKa = 11.84IGRR296 pKa = 11.84GEE298 pKa = 3.9ADD300 pKa = 3.13AVFSVYY306 pKa = 10.56GAGRR310 pKa = 11.84SLSTAGDD317 pKa = 3.9LNGDD321 pKa = 4.0GLNDD325 pKa = 3.85LVIGASDD332 pKa = 3.18MDD334 pKa = 4.23YY335 pKa = 11.34NGEE338 pKa = 4.03EE339 pKa = 3.86SGAAYY344 pKa = 10.73LLYY347 pKa = 10.84GKK349 pKa = 10.33AGDD352 pKa = 3.82NAWRR356 pKa = 11.84GEE358 pKa = 3.8IDD360 pKa = 4.68LDD362 pKa = 3.76NADD365 pKa = 3.37VRR367 pKa = 11.84FEE369 pKa = 4.08GVAEE373 pKa = 4.19GDD375 pKa = 3.29KK376 pKa = 11.06AGYY379 pKa = 8.81QVSSAGDD386 pKa = 3.5VNGDD390 pKa = 4.04GIDD393 pKa = 3.86DD394 pKa = 4.45LLIGAYY400 pKa = 8.56QAQGDD405 pKa = 4.27PNDD408 pKa = 3.99YY409 pKa = 11.44ADD411 pKa = 4.07GQQGGKK417 pKa = 8.3TYY419 pKa = 10.71LVYY422 pKa = 10.64GRR424 pKa = 11.84RR425 pKa = 11.84GGVLDD430 pKa = 3.77EE431 pKa = 5.04AVYY434 pKa = 11.13DD435 pKa = 4.53LDD437 pKa = 3.81NADD440 pKa = 3.64AVFIGGYY447 pKa = 9.49RR448 pKa = 11.84DD449 pKa = 4.47FIGSAVDD456 pKa = 3.35STGDD460 pKa = 3.66LNGDD464 pKa = 3.72GLADD468 pKa = 3.34IVLGAHH474 pKa = 6.94ASDD477 pKa = 3.52TVRR480 pKa = 11.84LSEE483 pKa = 3.84GAVYY487 pKa = 10.27IFYY490 pKa = 10.49GRR492 pKa = 11.84PVPEE496 pKa = 4.19PSAAVLTIAAAAAFSSTFRR515 pKa = 11.84GKK517 pKa = 9.31PVCQFSEE524 pKa = 4.23

Molecular weight: 54.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A518KC76|A0A518KC76_9BACT Uncharacterized protein OS=Botrimarina mediterranea OX=2528022 GN=Spa11_36170 PE=4 SV=1
MM1 pKa = 7.31NFSDD5 pKa = 3.99PNLWALLAWLFWCYY19 pKa = 10.77CFLDD23 pKa = 5.45FKK25 pKa = 10.79AHH27 pKa = 6.7RR28 pKa = 11.84YY29 pKa = 9.59DD30 pKa = 5.61RR31 pKa = 11.84IPDD34 pKa = 3.42DD35 pKa = 3.86RR36 pKa = 11.84LRR38 pKa = 11.84VALAEE43 pKa = 4.21HH44 pKa = 6.55EE45 pKa = 4.03AALRR49 pKa = 11.84KK50 pKa = 8.87EE51 pKa = 4.24AAGKK55 pKa = 10.22GDD57 pKa = 3.72PGRR60 pKa = 11.84VNRR63 pKa = 11.84AKK65 pKa = 10.16RR66 pKa = 11.84DD67 pKa = 3.37VVRR70 pKa = 11.84RR71 pKa = 11.84QKK73 pKa = 10.7AASANRR79 pKa = 11.84RR80 pKa = 11.84AHH82 pKa = 5.64QRR84 pKa = 11.84LHH86 pKa = 6.93QEE88 pKa = 3.62VAAANWPVLGRR99 pKa = 11.84VACALVASFLWCHH112 pKa = 6.15ILASVLEE119 pKa = 4.72CARR122 pKa = 11.84QTLPRR127 pKa = 11.84PVAPCVSTRR136 pKa = 11.84PPVSDD141 pKa = 4.69PSTQSTARR149 pKa = 11.84DD150 pKa = 3.38RR151 pKa = 11.84KK152 pKa = 10.54SPALCSSEE160 pKa = 5.09RR161 pKa = 11.84FAQRR165 pKa = 11.84RR166 pKa = 11.84RR167 pKa = 11.84GAPII171 pKa = 3.51

Molecular weight: 19.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4587

0

4587

1667008

29

5868

363.4

39.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.69 ± 0.044

1.022 ± 0.016

6.157 ± 0.035

6.344 ± 0.037

3.38 ± 0.022

8.489 ± 0.045

1.948 ± 0.02

4.189 ± 0.024

3.052 ± 0.032

9.86 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.992 ± 0.017

2.822 ± 0.032

5.412 ± 0.027

3.329 ± 0.022

6.898 ± 0.042

5.969 ± 0.032

5.614 ± 0.035

7.8 ± 0.027

1.542 ± 0.014

2.492 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski