Amino acid dipepetide frequency for Sphingomonas sp. Leaf231

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.065AlaAla: 23.065 ± 0.262
1.088AlaCys: 1.088 ± 0.035
8.149AlaAsp: 8.149 ± 0.095
7.217AlaGlu: 7.217 ± 0.119
4.34AlaPhe: 4.34 ± 0.072
13.061AlaGly: 13.061 ± 0.149
2.564AlaHis: 2.564 ± 0.052
7.052AlaIle: 7.052 ± 0.095
3.307AlaLys: 3.307 ± 0.069
15.003AlaLeu: 15.003 ± 0.15
4.04AlaMet: 4.04 ± 0.071
3.055AlaAsn: 3.055 ± 0.068
7.021AlaPro: 7.021 ± 0.101
4.67AlaGln: 4.67 ± 0.069
11.624AlaArg: 11.624 ± 0.15
6.413AlaSer: 6.413 ± 0.079
8.343AlaThr: 8.343 ± 0.088
9.761AlaVal: 9.761 ± 0.116
1.788AlaTrp: 1.788 ± 0.052
2.46AlaTyr: 2.46 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.953CysAla: 0.953 ± 0.034
0.083CysCys: 0.083 ± 0.011
0.491CysAsp: 0.491 ± 0.022
0.314CysGlu: 0.314 ± 0.017
0.26CysPhe: 0.26 ± 0.015
0.771CysGly: 0.771 ± 0.03
0.192CysHis: 0.192 ± 0.013
0.299CysIle: 0.299 ± 0.018
0.107CysLys: 0.107 ± 0.011
0.579CysLeu: 0.579 ± 0.026
0.122CysMet: 0.122 ± 0.01
0.168CysAsn: 0.168 ± 0.014
0.381CysPro: 0.381 ± 0.02
0.166CysGln: 0.166 ± 0.013
0.545CysArg: 0.545 ± 0.021
0.349CysSer: 0.349 ± 0.018
0.399CysThr: 0.399 ± 0.018
0.538CysVal: 0.538 ± 0.021
0.084CysTrp: 0.084 ± 0.01
0.141CysTyr: 0.141 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
9.239AspAla: 9.239 ± 0.111
0.386AspCys: 0.386 ± 0.018
3.834AspAsp: 3.834 ± 0.066
3.118AspGlu: 3.118 ± 0.059
1.983AspPhe: 1.983 ± 0.048
6.179AspGly: 6.179 ± 0.092
1.397AspHis: 1.397 ± 0.042
2.413AspIle: 2.413 ± 0.053
1.375AspLys: 1.375 ± 0.04
5.639AspLeu: 5.639 ± 0.071
1.334AspMet: 1.334 ± 0.041
1.254AspAsn: 1.254 ± 0.035
3.826AspPro: 3.826 ± 0.058
1.726AspGln: 1.726 ± 0.039
5.526AspArg: 5.526 ± 0.086
1.994AspSer: 1.994 ± 0.044
3.054AspThr: 3.054 ± 0.058
4.814AspVal: 4.814 ± 0.074
1.116AspTrp: 1.116 ± 0.034
1.564AspTyr: 1.564 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
7.066GluAla: 7.066 ± 0.101
0.252GluCys: 0.252 ± 0.015
2.358GluAsp: 2.358 ± 0.06
2.54GluGlu: 2.54 ± 0.06
1.22GluPhe: 1.22 ± 0.041
3.969GluGly: 3.969 ± 0.066
1.103GluHis: 1.103 ± 0.034
2.328GluIle: 2.328 ± 0.044
1.378GluLys: 1.378 ± 0.048
4.389GluLeu: 4.389 ± 0.074
1.237GluMet: 1.237 ± 0.036
1.1GluAsn: 1.1 ± 0.036
2.331GluPro: 2.331 ± 0.048
2.013GluGln: 2.013 ± 0.05
5.241GluArg: 5.241 ± 0.095
1.765GluSer: 1.765 ± 0.046
2.783GluThr: 2.783 ± 0.052
3.477GluVal: 3.477 ± 0.066
0.747GluTrp: 0.747 ± 0.03
0.892GluTyr: 0.892 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
5.014PheAla: 5.014 ± 0.079
0.258PheCys: 0.258 ± 0.017
2.668PheAsp: 2.668 ± 0.062
1.669PheGlu: 1.669 ± 0.042
1.18PhePhe: 1.18 ± 0.039
3.524PheGly: 3.524 ± 0.064
0.7PheHis: 0.7 ± 0.024
1.165PheIle: 1.165 ± 0.03
0.634PheLys: 0.634 ± 0.027
2.783PheLeu: 2.783 ± 0.061
0.682PheMet: 0.682 ± 0.024
0.931PheAsn: 0.931 ± 0.038
1.368PhePro: 1.368 ± 0.036
0.855PheGln: 0.855 ± 0.035
2.317PheArg: 2.317 ± 0.045
1.723PheSer: 1.723 ± 0.041
2.081PheThr: 2.081 ± 0.045
2.773PheVal: 2.773 ± 0.061
0.469PheTrp: 0.469 ± 0.024
0.885PheTyr: 0.885 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
11.197GlyAla: 11.197 ± 0.136
0.791GlyCys: 0.791 ± 0.027
5.44GlyAsp: 5.44 ± 0.067
4.642GlyGlu: 4.642 ± 0.065
3.596GlyPhe: 3.596 ± 0.066
8.756GlyGly: 8.756 ± 0.137
1.904GlyHis: 1.904 ± 0.039
4.184GlyIle: 4.184 ± 0.064
2.737GlyLys: 2.737 ± 0.053
8.167GlyLeu: 8.167 ± 0.097
2.362GlyMet: 2.362 ± 0.046
2.323GlyAsn: 2.323 ± 0.069
3.414GlyPro: 3.414 ± 0.05
2.929GlyGln: 2.929 ± 0.054
7.26GlyArg: 7.26 ± 0.093
4.643GlySer: 4.643 ± 0.085
5.492GlyThr: 5.492 ± 0.093
7.126GlyVal: 7.126 ± 0.091
1.732GlyTrp: 1.732 ± 0.047
2.463GlyTyr: 2.463 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
2.718HisAla: 2.718 ± 0.056
0.19HisCys: 0.19 ± 0.013
1.416HisAsp: 1.416 ± 0.042
1.004HisGlu: 1.004 ± 0.036
0.713HisPhe: 0.713 ± 0.026
2.027HisGly: 2.027 ± 0.049
0.615HisHis: 0.615 ± 0.025
0.728HisIle: 0.728 ± 0.027
0.332HisLys: 0.332 ± 0.018
1.884HisLeu: 1.884 ± 0.041
0.388HisMet: 0.388 ± 0.02
0.394HisAsn: 0.394 ± 0.019
1.279HisPro: 1.279 ± 0.037
0.515HisGln: 0.515 ± 0.02
1.619HisArg: 1.619 ± 0.039
0.736HisSer: 0.736 ± 0.026
0.717HisThr: 0.717 ± 0.027
1.605HisVal: 1.605 ± 0.038
0.36HisTrp: 0.36 ± 0.019
0.513HisTyr: 0.513 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
7.98IleAla: 7.98 ± 0.083
0.321IleCys: 0.321 ± 0.015
3.958IleAsp: 3.958 ± 0.067
3.018IleGlu: 3.018 ± 0.054
1.218IlePhe: 1.218 ± 0.037
5.011IleGly: 5.011 ± 0.079
0.784IleHis: 0.784 ± 0.03
1.429IleIle: 1.429 ± 0.044
0.923IleLys: 0.923 ± 0.031
3.219IleLeu: 3.219 ± 0.06
0.724IleMet: 0.724 ± 0.025
1.137IleAsn: 1.137 ± 0.031
1.937IlePro: 1.937 ± 0.046
0.933IleGln: 0.933 ± 0.032
3.021IleArg: 3.021 ± 0.055
1.901IleSer: 1.901 ± 0.047
2.365IleThr: 2.365 ± 0.054
4.438IleVal: 4.438 ± 0.07
0.496IleTrp: 0.496 ± 0.022
0.86IleTyr: 0.86 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
3.208LysAla: 3.208 ± 0.065
0.101LysCys: 0.101 ± 0.01
1.24LysAsp: 1.24 ± 0.039
0.947LysGlu: 0.947 ± 0.031
0.628LysPhe: 0.628 ± 0.029
2.131LysGly: 2.131 ± 0.047
0.388LysHis: 0.388 ± 0.02
1.001LysIle: 1.001 ± 0.035
0.81LysLys: 0.81 ± 0.038
2.611LysLeu: 2.611 ± 0.053
0.623LysMet: 0.623 ± 0.027
0.527LysAsn: 0.527 ± 0.025
1.618LysPro: 1.618 ± 0.042
0.722LysGln: 0.722 ± 0.029
1.909LysArg: 1.909 ± 0.045
1.182LysSer: 1.182 ± 0.036
1.343LysThr: 1.343 ± 0.043
1.92LysVal: 1.92 ± 0.05
0.321LysTrp: 0.321 ± 0.017
0.483LysTyr: 0.483 ± 0.022
0.0LysXaa: 0.0 ± 0.0
Leu
14.721LeuAla: 14.721 ± 0.154
0.693LeuCys: 0.693 ± 0.024
6.312LeuAsp: 6.312 ± 0.083
4.01LeuGlu: 4.01 ± 0.064
3.411LeuPhe: 3.411 ± 0.07
8.197LeuGly: 8.197 ± 0.106
1.857LeuHis: 1.857 ± 0.043
4.292LeuIle: 4.292 ± 0.068
2.377LeuLys: 2.377 ± 0.049
9.44LeuLeu: 9.44 ± 0.126
1.991LeuMet: 1.991 ± 0.042
2.261LeuAsn: 2.261 ± 0.055
5.8LeuPro: 5.8 ± 0.079
2.186LeuGln: 2.186 ± 0.05
7.381LeuArg: 7.381 ± 0.083
5.677LeuSer: 5.677 ± 0.071
5.969LeuThr: 5.969 ± 0.075
7.375LeuVal: 7.375 ± 0.082
1.23LeuTrp: 1.23 ± 0.04
1.908LeuTyr: 1.908 ± 0.037
0.0LeuXaa: 0.0 ± 0.0
Met
3.144MetAla: 3.144 ± 0.054
0.143MetCys: 0.143 ± 0.011
0.97MetAsp: 0.97 ± 0.03
0.909MetGlu: 0.909 ± 0.036
0.652MetPhe: 0.652 ± 0.026
1.657MetGly: 1.657 ± 0.042
0.38MetHis: 0.38 ± 0.019
1.266MetIle: 1.266 ± 0.037
0.794MetLys: 0.794 ± 0.027
2.555MetLeu: 2.555 ± 0.053
0.648MetMet: 0.648 ± 0.026
0.643MetAsn: 0.643 ± 0.023
1.456MetPro: 1.456 ± 0.039
0.698MetGln: 0.698 ± 0.025
1.826MetArg: 1.826 ± 0.039
1.24MetSer: 1.24 ± 0.039
1.916MetThr: 1.916 ± 0.041
1.696MetVal: 1.696 ± 0.039
0.268MetTrp: 0.268 ± 0.016
0.263MetTyr: 0.263 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
3.237AsnAla: 3.237 ± 0.061
0.165AsnCys: 0.165 ± 0.012
1.56AsnAsp: 1.56 ± 0.04
1.011AsnGlu: 1.011 ± 0.031
0.788AsnPhe: 0.788 ± 0.035
2.434AsnGly: 2.434 ± 0.063
0.435AsnHis: 0.435 ± 0.02
1.146AsnIle: 1.146 ± 0.037
0.515AsnLys: 0.515 ± 0.023
2.251AsnLeu: 2.251 ± 0.047
0.451AsnMet: 0.451 ± 0.021
0.653AsnAsn: 0.653 ± 0.025
1.571AsnPro: 1.571 ± 0.047
0.653AsnGln: 0.653 ± 0.026
1.69AsnArg: 1.69 ± 0.047
1.042AsnSer: 1.042 ± 0.041
1.216AsnThr: 1.216 ± 0.039
1.924AsnVal: 1.924 ± 0.048
0.374AsnTrp: 0.374 ± 0.016
0.66AsnTyr: 0.66 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
8.11ProAla: 8.11 ± 0.114
0.299ProCys: 0.299 ± 0.019
3.749ProAsp: 3.749 ± 0.061
2.81ProGlu: 2.81 ± 0.053
1.889ProPhe: 1.889 ± 0.042
5.102ProGly: 5.102 ± 0.076
1.033ProHis: 1.033 ± 0.032
2.26ProIle: 2.26 ± 0.049
1.12ProLys: 1.12 ± 0.033
5.036ProLeu: 5.036 ± 0.066
1.119ProMet: 1.119 ± 0.034
1.219ProAsn: 1.219 ± 0.036
2.863ProPro: 2.863 ± 0.071
1.571ProGln: 1.571 ± 0.039
3.514ProArg: 3.514 ± 0.07
2.584ProSer: 2.584 ± 0.056
2.726ProThr: 2.726 ± 0.051
4.547ProVal: 4.547 ± 0.066
0.723ProTrp: 0.723 ± 0.024
1.088ProTyr: 1.088 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
4.265GlnAla: 4.265 ± 0.068
0.179GlnCys: 0.179 ± 0.014
1.329GlnAsp: 1.329 ± 0.034
1.135GlnGlu: 1.135 ± 0.037
0.985GlnPhe: 0.985 ± 0.036
2.271GlnGly: 2.271 ± 0.042
0.598GlnHis: 0.598 ± 0.021
1.453GlnIle: 1.453 ± 0.046
0.708GlnLys: 0.708 ± 0.031
2.96GlnLeu: 2.96 ± 0.057
0.713GlnMet: 0.713 ± 0.026
0.715GlnAsn: 0.715 ± 0.031
1.808GlnPro: 1.808 ± 0.047
1.235GlnGln: 1.235 ± 0.042
2.787GlnArg: 2.787 ± 0.056
1.506GlnSer: 1.506 ± 0.039
1.603GlnThr: 1.603 ± 0.047
2.485GlnVal: 2.485 ± 0.047
0.407GlnTrp: 0.407 ± 0.02
0.594GlnTyr: 0.594 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
10.509ArgAla: 10.509 ± 0.141
0.501ArgCys: 0.501 ± 0.025
5.122ArgAsp: 5.122 ± 0.074
3.889ArgGlu: 3.889 ± 0.073
3.295ArgPhe: 3.295 ± 0.056
5.914ArgGly: 5.914 ± 0.074
1.871ArgHis: 1.871 ± 0.046
4.162ArgIle: 4.162 ± 0.06
1.617ArgLys: 1.617 ± 0.041
8.801ArgLeu: 8.801 ± 0.11
2.022ArgMet: 2.022 ± 0.044
1.841ArgAsn: 1.841 ± 0.048
4.028ArgPro: 4.028 ± 0.072
2.461ArgGln: 2.461 ± 0.058
6.925ArgArg: 6.925 ± 0.102
3.715ArgSer: 3.715 ± 0.06
4.22ArgThr: 4.22 ± 0.065
5.947ArgVal: 5.947 ± 0.09
1.429ArgTrp: 1.429 ± 0.035
2.048ArgTyr: 2.048 ± 0.048
0.001ArgXaa: 0.001 ± 0.001
Ser
6.27SerAla: 6.27 ± 0.077
0.339SerCys: 0.339 ± 0.021
2.817SerAsp: 2.817 ± 0.054
2.016SerGlu: 2.016 ± 0.047
1.936SerPhe: 1.936 ± 0.043
5.228SerGly: 5.228 ± 0.08
0.804SerHis: 0.804 ± 0.027
2.434SerIle: 2.434 ± 0.055
1.046SerLys: 1.046 ± 0.033
4.46SerLeu: 4.46 ± 0.06
1.01SerMet: 1.01 ± 0.033
1.217SerAsn: 1.217 ± 0.039
2.702SerPro: 2.702 ± 0.052
1.285SerGln: 1.285 ± 0.04
3.377SerArg: 3.377 ± 0.055
2.463SerSer: 2.463 ± 0.057
2.653SerThr: 2.653 ± 0.058
3.573SerVal: 3.573 ± 0.063
0.674SerTrp: 0.674 ± 0.024
1.281SerTyr: 1.281 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
7.21ThrAla: 7.21 ± 0.084
0.368ThrCys: 0.368 ± 0.022
3.058ThrAsp: 3.058 ± 0.052
2.128ThrGlu: 2.128 ± 0.042
2.008ThrPhe: 2.008 ± 0.049
5.885ThrGly: 5.885 ± 0.082
0.95ThrHis: 0.95 ± 0.03
3.253ThrIle: 3.253 ± 0.064
1.202ThrLys: 1.202 ± 0.034
6.551ThrLeu: 6.551 ± 0.093
1.296ThrMet: 1.296 ± 0.032
1.308ThrAsn: 1.308 ± 0.045
3.87ThrPro: 3.87 ± 0.067
1.613ThrGln: 1.613 ± 0.04
4.227ThrArg: 4.227 ± 0.054
2.732ThrSer: 2.732 ± 0.059
3.162ThrThr: 3.162 ± 0.069
4.462ThrVal: 4.462 ± 0.072
0.712ThrTrp: 0.712 ± 0.026
1.185ThrTyr: 1.185 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
11.866ValAla: 11.866 ± 0.148
0.488ValCys: 0.488 ± 0.021
4.904ValAsp: 4.904 ± 0.074
4.283ValGlu: 4.283 ± 0.066
2.223ValPhe: 2.223 ± 0.045
5.948ValGly: 5.948 ± 0.077
1.347ValHis: 1.347 ± 0.036
3.457ValIle: 3.457 ± 0.061
1.839ValLys: 1.839 ± 0.045
6.989ValLeu: 6.989 ± 0.085
1.66ValMet: 1.66 ± 0.044
1.956ValAsn: 1.956 ± 0.049
4.342ValPro: 4.342 ± 0.065
2.148ValGln: 2.148 ± 0.046
6.025ValArg: 6.025 ± 0.074
3.997ValSer: 3.997 ± 0.065
5.233ValThr: 5.233 ± 0.077
6.09ValVal: 6.09 ± 0.093
0.842ValTrp: 0.842 ± 0.026
1.369ValTyr: 1.369 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.399TrpAla: 1.399 ± 0.04
0.12TrpCys: 0.12 ± 0.009
0.763TrpAsp: 0.763 ± 0.028
0.503TrpGlu: 0.503 ± 0.02
0.554TrpPhe: 0.554 ± 0.022
0.959TrpGly: 0.959 ± 0.03
0.371TrpHis: 0.371 ± 0.02
0.65TrpIle: 0.65 ± 0.026
0.403TrpLys: 0.403 ± 0.019
1.712TrpLeu: 1.712 ± 0.049
0.337TrpMet: 0.337 ± 0.017
0.439TrpAsn: 0.439 ± 0.019
0.756TrpPro: 0.756 ± 0.028
0.682TrpGln: 0.682 ± 0.028
1.491TrpArg: 1.491 ± 0.042
0.884TrpSer: 0.884 ± 0.029
0.905TrpThr: 0.905 ± 0.031
0.863TrpVal: 0.863 ± 0.03
0.304TrpTrp: 0.304 ± 0.017
0.306TrpTyr: 0.306 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.851TyrAla: 2.851 ± 0.056
0.175TyrCys: 0.175 ± 0.014
1.636TyrAsp: 1.636 ± 0.042
1.024TyrGlu: 1.024 ± 0.033
0.8TyrPhe: 0.8 ± 0.026
2.135TyrGly: 2.135 ± 0.057
0.461TyrHis: 0.461 ± 0.021
0.7TyrIle: 0.7 ± 0.026
0.489TyrLys: 0.489 ± 0.021
2.056TyrLeu: 2.056 ± 0.054
0.364TyrMet: 0.364 ± 0.019
0.635TyrAsn: 0.635 ± 0.031
0.991TyrPro: 0.991 ± 0.032
0.674TyrGln: 0.674 ± 0.026
2.009TyrArg: 2.009 ± 0.052
1.04TyrSer: 1.04 ± 0.037
1.001TyrThr: 1.001 ± 0.034
1.594TyrVal: 1.594 ± 0.038
0.326TyrTrp: 0.326 ± 0.018
0.571TyrTyr: 0.571 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.001XaaLeu: 0.001 ± 0.001
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3320 proteins (1087510 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski