Ralstonia phage RSJ5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Okabevirinae; Risjevirus; Ralstonia virus RSJ5

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077KVQ4|A0A077KVQ4_9CAUD Putative internal virion protein OS=Ralstonia phage RSJ5 OX=1538364 PE=4 SV=1
MM1 pKa = 6.51TTEE4 pKa = 4.01ATQAAAASADD14 pKa = 3.6QTAATAAATTATSGTPATQQSAAPATPAANPAAAQVKK51 pKa = 10.17DD52 pKa = 3.7LGIGTGEE59 pKa = 4.05NAAGPGKK66 pKa = 10.0AANGIEE72 pKa = 4.53YY73 pKa = 10.61YY74 pKa = 10.6SEE76 pKa = 3.65TGYY79 pKa = 11.46AGLDD83 pKa = 3.2AALKK87 pKa = 10.22FMVDD91 pKa = 2.95AGLNDD96 pKa = 3.79DD97 pKa = 4.58HH98 pKa = 6.99PAIKK102 pKa = 10.21AVEE105 pKa = 4.16KK106 pKa = 11.1NDD108 pKa = 3.49FSLLEE113 pKa = 4.66AYY115 pKa = 8.83FATNPVAGWEE125 pKa = 4.23TMLKK129 pKa = 9.67IGRR132 pKa = 11.84EE133 pKa = 4.01GFEE136 pKa = 4.63RR137 pKa = 11.84MLKK140 pKa = 10.58DD141 pKa = 3.43VGEE144 pKa = 4.43KK145 pKa = 10.33GAALEE150 pKa = 4.19RR151 pKa = 11.84TNKK154 pKa = 9.78EE155 pKa = 3.25MGVALFGDD163 pKa = 4.27EE164 pKa = 4.19ATLNAALDD172 pKa = 4.14YY173 pKa = 10.79FPTIPGIEE181 pKa = 4.06QADD184 pKa = 3.7VDD186 pKa = 4.54GVQAMIRR193 pKa = 11.84AGGVQAQAAMLLIQDD208 pKa = 4.25QFNAAQGVTRR218 pKa = 11.84APLEE222 pKa = 4.47DD223 pKa = 3.4VSQRR227 pKa = 11.84TATYY231 pKa = 10.19QSQGDD236 pKa = 4.21HH237 pKa = 6.64SPLSARR243 pKa = 11.84QFTEE247 pKa = 3.86EE248 pKa = 3.87CHH250 pKa = 6.05KK251 pKa = 10.64LRR253 pKa = 11.84RR254 pKa = 11.84ALGDD258 pKa = 3.49SFEE261 pKa = 4.15QTPQYY266 pKa = 10.12RR267 pKa = 11.84ALAARR272 pKa = 11.84VRR274 pKa = 11.84RR275 pKa = 3.95

Molecular weight: 28.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077KYK0|A0A077KYK0_9CAUD DNA-directed RNA polymerase OS=Ralstonia phage RSJ5 OX=1538364 PE=3 SV=1
MM1 pKa = 7.3SRR3 pKa = 11.84TVSTRR8 pKa = 11.84LRR10 pKa = 11.84DD11 pKa = 4.13GKK13 pKa = 8.07WTSPTRR19 pKa = 11.84YY20 pKa = 8.91WITAIPTGEE29 pKa = 3.97FEE31 pKa = 4.14RR32 pKa = 11.84RR33 pKa = 11.84SVGGTMVFLAKK44 pKa = 10.29SRR46 pKa = 11.84TGRR49 pKa = 11.84TVWALTLPVAKK60 pKa = 9.51GWHH63 pKa = 6.65RR64 pKa = 11.84GRR66 pKa = 11.84ISATSRR72 pKa = 11.84TTLRR76 pKa = 11.84STPSLSTSTAIRR88 pKa = 11.84RR89 pKa = 11.84TRR91 pKa = 11.84LNARR95 pKa = 11.84RR96 pKa = 11.84WTT98 pKa = 3.58

Molecular weight: 11.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

12386

75

1372

302.1

33.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.739 ± 0.704

0.824 ± 0.164

6.273 ± 0.187

5.619 ± 0.368

3.359 ± 0.183

7.807 ± 0.333

1.962 ± 0.197

4.392 ± 0.222

5.256 ± 0.243

7.686 ± 0.379

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.777 ± 0.205

4.287 ± 0.238

4.295 ± 0.24

4.449 ± 0.316

6.031 ± 0.272

5.765 ± 0.303

6.007 ± 0.409

6.556 ± 0.306

1.704 ± 0.178

3.213 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski