Amino acid dipepetide frequency for Lactococcus phage proPhi7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.29AlaAla: 3.29 ± 0.892
0.606AlaCys: 0.606 ± 0.197
3.809AlaAsp: 3.809 ± 0.588
3.809AlaGlu: 3.809 ± 0.682
2.251AlaPhe: 2.251 ± 0.425
4.588AlaGly: 4.588 ± 0.91
1.299AlaHis: 1.299 ± 0.372
5.541AlaIle: 5.541 ± 0.923
6.233AlaLys: 6.233 ± 0.87
5.974AlaLeu: 5.974 ± 0.853
1.645AlaMet: 1.645 ± 0.287
3.376AlaAsn: 3.376 ± 0.552
1.385AlaPro: 1.385 ± 0.379
2.078AlaGln: 2.078 ± 0.381
3.117AlaArg: 3.117 ± 0.569
3.203AlaSer: 3.203 ± 0.57
3.809AlaThr: 3.809 ± 0.532
3.549AlaVal: 3.549 ± 0.619
1.039AlaTrp: 1.039 ± 0.258
1.645AlaTyr: 1.645 ± 0.424
0.0AlaXaa: 0.0 ± 0.0
Cys
0.346CysAla: 0.346 ± 0.181
0.0CysCys: 0.0 ± 0.0
1.125CysAsp: 1.125 ± 0.308
0.173CysGlu: 0.173 ± 0.113
0.433CysPhe: 0.433 ± 0.217
0.519CysGly: 0.519 ± 0.252
0.173CysHis: 0.173 ± 0.124
0.346CysIle: 0.346 ± 0.162
0.346CysLys: 0.346 ± 0.195
0.346CysLeu: 0.346 ± 0.156
0.087CysMet: 0.087 ± 0.08
0.26CysAsn: 0.26 ± 0.145
0.26CysPro: 0.26 ± 0.145
0.346CysGln: 0.346 ± 0.156
0.433CysArg: 0.433 ± 0.212
0.346CysSer: 0.346 ± 0.152
0.346CysThr: 0.346 ± 0.198
0.173CysVal: 0.173 ± 0.132
0.087CysTrp: 0.087 ± 0.077
0.346CysTyr: 0.346 ± 0.148
0.0CysXaa: 0.0 ± 0.0
Asp
2.597AspAla: 2.597 ± 0.506
0.26AspCys: 0.26 ± 0.147
3.463AspAsp: 3.463 ± 0.639
5.194AspGlu: 5.194 ± 0.639
2.943AspPhe: 2.943 ± 0.449
4.242AspGly: 4.242 ± 0.595
0.779AspHis: 0.779 ± 0.21
4.415AspIle: 4.415 ± 0.629
3.636AspLys: 3.636 ± 0.636
5.021AspLeu: 5.021 ± 0.699
1.645AspMet: 1.645 ± 0.398
4.069AspAsn: 4.069 ± 0.634
1.212AspPro: 1.212 ± 0.382
1.299AspGln: 1.299 ± 0.318
3.203AspArg: 3.203 ± 0.517
4.155AspSer: 4.155 ± 0.642
3.117AspThr: 3.117 ± 0.634
3.463AspVal: 3.463 ± 0.667
1.039AspTrp: 1.039 ± 0.316
2.77AspTyr: 2.77 ± 0.408
0.0AspXaa: 0.0 ± 0.0
Glu
5.368GluAla: 5.368 ± 0.569
0.346GluCys: 0.346 ± 0.194
3.549GluAsp: 3.549 ± 0.755
4.155GluGlu: 4.155 ± 0.75
4.069GluPhe: 4.069 ± 0.571
2.77GluGly: 2.77 ± 0.501
1.299GluHis: 1.299 ± 0.296
5.541GluIle: 5.541 ± 0.658
6.233GluLys: 6.233 ± 0.849
7.099GluLeu: 7.099 ± 0.98
2.078GluMet: 2.078 ± 0.393
3.896GluAsn: 3.896 ± 0.583
1.905GluPro: 1.905 ± 0.444
3.03GluGln: 3.03 ± 0.681
3.809GluArg: 3.809 ± 0.541
3.896GluSer: 3.896 ± 0.576
4.502GluThr: 4.502 ± 0.591
4.069GluVal: 4.069 ± 0.573
0.866GluTrp: 0.866 ± 0.309
1.991GluTyr: 1.991 ± 0.529
0.0GluXaa: 0.0 ± 0.0
Phe
2.511PheAla: 2.511 ± 0.5
0.433PheCys: 0.433 ± 0.205
3.29PheAsp: 3.29 ± 0.442
3.809PheGlu: 3.809 ± 0.669
1.212PhePhe: 1.212 ± 0.239
2.943PheGly: 2.943 ± 0.508
1.039PheHis: 1.039 ± 0.284
2.164PheIle: 2.164 ± 0.414
4.242PheLys: 4.242 ± 0.484
2.597PheLeu: 2.597 ± 0.577
0.952PheMet: 0.952 ± 0.259
2.943PheAsn: 2.943 ± 0.522
1.299PhePro: 1.299 ± 0.292
1.212PheGln: 1.212 ± 0.291
1.731PheArg: 1.731 ± 0.409
2.943PheSer: 2.943 ± 0.607
3.636PheThr: 3.636 ± 0.478
2.684PheVal: 2.684 ± 0.461
0.346PheTrp: 0.346 ± 0.169
1.212PheTyr: 1.212 ± 0.305
0.0PheXaa: 0.0 ± 0.0
Gly
3.463GlyAla: 3.463 ± 0.726
0.433GlyCys: 0.433 ± 0.182
3.463GlyAsp: 3.463 ± 0.423
3.03GlyGlu: 3.03 ± 0.391
3.117GlyPhe: 3.117 ± 0.413
4.415GlyGly: 4.415 ± 0.836
0.952GlyHis: 0.952 ± 0.247
6.753GlyIle: 6.753 ± 1.101
6.406GlyLys: 6.406 ± 0.7
5.108GlyLeu: 5.108 ± 0.652
1.299GlyMet: 1.299 ± 0.362
4.069GlyAsn: 4.069 ± 0.562
0.866GlyPro: 0.866 ± 0.38
2.511GlyGln: 2.511 ± 0.562
1.991GlyArg: 1.991 ± 0.394
3.549GlySer: 3.549 ± 0.695
4.761GlyThr: 4.761 ± 0.647
3.376GlyVal: 3.376 ± 0.406
0.779GlyTrp: 0.779 ± 0.263
3.636GlyTyr: 3.636 ± 0.579
0.0GlyXaa: 0.0 ± 0.0
His
0.779HisAla: 0.779 ± 0.256
0.0HisCys: 0.0 ± 0.0
0.693HisAsp: 0.693 ± 0.252
1.385HisGlu: 1.385 ± 0.375
0.346HisPhe: 0.346 ± 0.162
0.779HisGly: 0.779 ± 0.414
0.26HisHis: 0.26 ± 0.118
1.299HisIle: 1.299 ± 0.429
0.866HisLys: 0.866 ± 0.279
1.558HisLeu: 1.558 ± 0.438
0.087HisMet: 0.087 ± 0.077
0.693HisAsn: 0.693 ± 0.221
0.346HisPro: 0.346 ± 0.163
0.952HisGln: 0.952 ± 0.267
0.606HisArg: 0.606 ± 0.207
0.779HisSer: 0.779 ± 0.276
0.606HisThr: 0.606 ± 0.181
0.779HisVal: 0.779 ± 0.241
0.26HisTrp: 0.26 ± 0.142
0.606HisTyr: 0.606 ± 0.222
0.0HisXaa: 0.0 ± 0.0
Ile
4.502IleAla: 4.502 ± 0.683
0.433IleCys: 0.433 ± 0.184
5.368IleAsp: 5.368 ± 0.531
5.194IleGlu: 5.194 ± 0.704
2.857IlePhe: 2.857 ± 0.451
3.982IleGly: 3.982 ± 0.583
0.779IleHis: 0.779 ± 0.253
5.627IleIle: 5.627 ± 0.618
5.887IleLys: 5.887 ± 0.539
5.281IleLeu: 5.281 ± 0.71
1.299IleMet: 1.299 ± 0.31
4.415IleAsn: 4.415 ± 0.485
2.77IlePro: 2.77 ± 0.358
2.857IleGln: 2.857 ± 0.539
2.251IleArg: 2.251 ± 0.372
7.359IleSer: 7.359 ± 1.21
5.368IleThr: 5.368 ± 0.885
4.502IleVal: 4.502 ± 0.65
0.606IleTrp: 0.606 ± 0.207
3.03IleTyr: 3.03 ± 0.485
0.0IleXaa: 0.0 ± 0.0
Lys
6.406LysAla: 6.406 ± 0.693
0.346LysCys: 0.346 ± 0.169
3.29LysAsp: 3.29 ± 0.475
6.147LysGlu: 6.147 ± 0.814
1.991LysPhe: 1.991 ± 0.379
6.06LysGly: 6.06 ± 0.698
1.125LysHis: 1.125 ± 0.308
6.147LysIle: 6.147 ± 0.781
8.138LysLys: 8.138 ± 1.331
6.926LysLeu: 6.926 ± 0.642
1.731LysMet: 1.731 ± 0.398
5.974LysAsn: 5.974 ± 0.666
3.376LysPro: 3.376 ± 0.511
3.636LysGln: 3.636 ± 0.592
3.809LysArg: 3.809 ± 0.5
6.06LysSer: 6.06 ± 0.47
6.06LysThr: 6.06 ± 0.845
4.415LysVal: 4.415 ± 0.767
1.299LysTrp: 1.299 ± 0.311
3.376LysTyr: 3.376 ± 0.597
0.0LysXaa: 0.0 ± 0.0
Leu
6.32LeuAla: 6.32 ± 0.795
0.346LeuCys: 0.346 ± 0.168
4.675LeuAsp: 4.675 ± 0.703
5.714LeuGlu: 5.714 ± 0.753
3.982LeuPhe: 3.982 ± 0.601
5.021LeuGly: 5.021 ± 0.619
0.779LeuHis: 0.779 ± 0.257
4.935LeuIle: 4.935 ± 0.543
9.35LeuLys: 9.35 ± 0.991
6.58LeuLeu: 6.58 ± 0.959
1.558LeuMet: 1.558 ± 0.294
5.714LeuAsn: 5.714 ± 0.7
3.549LeuPro: 3.549 ± 0.436
3.203LeuGln: 3.203 ± 0.613
3.29LeuArg: 3.29 ± 0.659
7.705LeuSer: 7.705 ± 0.678
5.021LeuThr: 5.021 ± 0.605
4.069LeuVal: 4.069 ± 0.657
0.952LeuTrp: 0.952 ± 0.344
1.905LeuTyr: 1.905 ± 0.394
0.0LeuXaa: 0.0 ± 0.0
Met
1.472MetAla: 1.472 ± 0.327
0.433MetCys: 0.433 ± 0.225
1.558MetAsp: 1.558 ± 0.362
2.164MetGlu: 2.164 ± 0.356
0.173MetPhe: 0.173 ± 0.127
0.952MetGly: 0.952 ± 0.295
0.26MetHis: 0.26 ± 0.146
1.125MetIle: 1.125 ± 0.286
1.731MetLys: 1.731 ± 0.363
1.731MetLeu: 1.731 ± 0.397
0.519MetMet: 0.519 ± 0.187
0.866MetAsn: 0.866 ± 0.266
0.952MetPro: 0.952 ± 0.287
0.779MetGln: 0.779 ± 0.263
1.039MetArg: 1.039 ± 0.319
1.818MetSer: 1.818 ± 0.392
1.905MetThr: 1.905 ± 0.386
0.866MetVal: 0.866 ± 0.286
0.346MetTrp: 0.346 ± 0.158
0.519MetTyr: 0.519 ± 0.21
0.0MetXaa: 0.0 ± 0.0
Asn
4.069AsnAla: 4.069 ± 0.632
0.346AsnCys: 0.346 ± 0.145
2.943AsnAsp: 2.943 ± 0.465
3.376AsnGlu: 3.376 ± 0.525
2.943AsnPhe: 2.943 ± 0.445
5.281AsnGly: 5.281 ± 0.673
0.606AsnHis: 0.606 ± 0.284
4.675AsnIle: 4.675 ± 0.636
4.675AsnLys: 4.675 ± 0.771
4.329AsnLeu: 4.329 ± 0.625
1.212AsnMet: 1.212 ± 0.272
3.203AsnAsn: 3.203 ± 0.555
2.251AsnPro: 2.251 ± 0.42
2.597AsnGln: 2.597 ± 0.552
1.991AsnArg: 1.991 ± 0.468
4.069AsnSer: 4.069 ± 0.615
2.337AsnThr: 2.337 ± 0.454
4.242AsnVal: 4.242 ± 0.483
0.693AsnTrp: 0.693 ± 0.243
2.684AsnTyr: 2.684 ± 0.52
0.0AsnXaa: 0.0 ± 0.0
Pro
0.693ProAla: 0.693 ± 0.343
0.0ProCys: 0.0 ± 0.0
1.731ProAsp: 1.731 ± 0.479
2.251ProGlu: 2.251 ± 0.435
1.818ProPhe: 1.818 ± 0.333
1.299ProGly: 1.299 ± 0.351
0.346ProHis: 0.346 ± 0.17
2.251ProIle: 2.251 ± 0.378
3.117ProLys: 3.117 ± 0.508
3.463ProLeu: 3.463 ± 0.527
0.779ProMet: 0.779 ± 0.235
2.164ProAsn: 2.164 ± 0.532
0.866ProPro: 0.866 ± 0.212
1.558ProGln: 1.558 ± 0.328
1.039ProArg: 1.039 ± 0.279
2.078ProSer: 2.078 ± 0.349
2.511ProThr: 2.511 ± 0.445
2.424ProVal: 2.424 ± 0.47
0.346ProTrp: 0.346 ± 0.177
0.779ProTyr: 0.779 ± 0.228
0.0ProXaa: 0.0 ± 0.0
Gln
3.723GlnAla: 3.723 ± 0.51
0.173GlnCys: 0.173 ± 0.11
1.299GlnAsp: 1.299 ± 0.35
3.896GlnGlu: 3.896 ± 0.497
1.905GlnPhe: 1.905 ± 0.368
1.731GlnGly: 1.731 ± 0.39
0.346GlnHis: 0.346 ± 0.223
2.251GlnIle: 2.251 ± 0.425
3.376GlnLys: 3.376 ± 0.513
3.982GlnLeu: 3.982 ± 0.644
0.433GlnMet: 0.433 ± 0.231
1.385GlnAsn: 1.385 ± 0.356
1.558GlnPro: 1.558 ± 0.263
1.212GlnGln: 1.212 ± 0.281
1.385GlnArg: 1.385 ± 0.271
3.29GlnSer: 3.29 ± 0.585
1.991GlnThr: 1.991 ± 0.457
2.251GlnVal: 2.251 ± 0.409
0.606GlnTrp: 0.606 ± 0.205
1.645GlnTyr: 1.645 ± 0.371
0.0GlnXaa: 0.0 ± 0.0
Arg
2.597ArgAla: 2.597 ± 0.467
0.346ArgCys: 0.346 ± 0.204
2.597ArgAsp: 2.597 ± 0.529
2.857ArgGlu: 2.857 ± 0.49
2.164ArgPhe: 2.164 ± 0.472
1.299ArgGly: 1.299 ± 0.387
0.346ArgHis: 0.346 ± 0.161
2.684ArgIle: 2.684 ± 0.412
3.463ArgLys: 3.463 ± 0.642
4.329ArgLeu: 4.329 ± 0.593
1.125ArgMet: 1.125 ± 0.367
2.511ArgAsn: 2.511 ± 0.702
1.299ArgPro: 1.299 ± 0.368
1.385ArgGln: 1.385 ± 0.279
2.511ArgArg: 2.511 ± 0.609
2.684ArgSer: 2.684 ± 0.482
2.424ArgThr: 2.424 ± 0.388
2.078ArgVal: 2.078 ± 0.474
0.693ArgTrp: 0.693 ± 0.225
2.078ArgTyr: 2.078 ± 0.375
0.0ArgXaa: 0.0 ± 0.0
Ser
5.021SerAla: 5.021 ± 0.774
0.606SerCys: 0.606 ± 0.223
3.809SerAsp: 3.809 ± 0.485
4.329SerGlu: 4.329 ± 0.539
2.424SerPhe: 2.424 ± 0.429
6.406SerGly: 6.406 ± 1.116
1.212SerHis: 1.212 ± 0.401
5.368SerIle: 5.368 ± 0.58
4.935SerLys: 4.935 ± 0.561
6.406SerLeu: 6.406 ± 0.936
1.905SerMet: 1.905 ± 0.412
4.242SerAsn: 4.242 ± 0.688
1.818SerPro: 1.818 ± 0.399
2.857SerGln: 2.857 ± 0.485
2.164SerArg: 2.164 ± 0.427
4.502SerSer: 4.502 ± 0.792
4.242SerThr: 4.242 ± 0.796
4.675SerVal: 4.675 ± 0.616
1.039SerTrp: 1.039 ± 0.359
3.463SerTyr: 3.463 ± 0.527
0.0SerXaa: 0.0 ± 0.0
Thr
3.636ThrAla: 3.636 ± 0.484
0.433ThrCys: 0.433 ± 0.204
3.29ThrAsp: 3.29 ± 0.57
4.415ThrGlu: 4.415 ± 0.525
2.164ThrPhe: 2.164 ± 0.434
5.194ThrGly: 5.194 ± 0.79
0.952ThrHis: 0.952 ± 0.346
5.281ThrIle: 5.281 ± 0.704
4.242ThrLys: 4.242 ± 0.523
5.454ThrLeu: 5.454 ± 0.718
0.952ThrMet: 0.952 ± 0.288
2.77ThrAsn: 2.77 ± 0.596
2.337ThrPro: 2.337 ± 0.415
2.511ThrGln: 2.511 ± 0.492
1.558ThrArg: 1.558 ± 0.363
4.675ThrSer: 4.675 ± 0.748
3.982ThrThr: 3.982 ± 0.688
6.233ThrVal: 6.233 ± 0.804
0.693ThrTrp: 0.693 ± 0.235
1.905ThrTyr: 1.905 ± 0.497
0.0ThrXaa: 0.0 ± 0.0
Val
2.943ValAla: 2.943 ± 0.52
0.346ValCys: 0.346 ± 0.227
4.675ValAsp: 4.675 ± 0.721
4.502ValGlu: 4.502 ± 0.686
3.636ValPhe: 3.636 ± 0.507
3.376ValGly: 3.376 ± 0.641
0.606ValHis: 0.606 ± 0.233
4.502ValIle: 4.502 ± 0.685
5.281ValLys: 5.281 ± 0.61
4.502ValLeu: 4.502 ± 0.599
1.299ValMet: 1.299 ± 0.256
2.77ValAsn: 2.77 ± 0.618
2.511ValPro: 2.511 ± 0.491
1.905ValGln: 1.905 ± 0.334
2.943ValArg: 2.943 ± 0.389
4.242ValSer: 4.242 ± 0.622
3.549ValThr: 3.549 ± 0.588
4.069ValVal: 4.069 ± 0.563
0.693ValTrp: 0.693 ± 0.274
1.991ValTyr: 1.991 ± 0.458
0.0ValXaa: 0.0 ± 0.0
Trp
0.693TrpAla: 0.693 ± 0.269
0.433TrpCys: 0.433 ± 0.193
0.952TrpAsp: 0.952 ± 0.27
1.039TrpGlu: 1.039 ± 0.289
0.693TrpPhe: 0.693 ± 0.235
0.779TrpGly: 0.779 ± 0.303
0.087TrpHis: 0.087 ± 0.072
0.693TrpIle: 0.693 ± 0.217
0.779TrpLys: 0.779 ± 0.294
1.125TrpLeu: 1.125 ± 0.354
0.087TrpMet: 0.087 ± 0.09
0.866TrpAsn: 0.866 ± 0.27
0.173TrpPro: 0.173 ± 0.116
0.779TrpGln: 0.779 ± 0.227
0.606TrpArg: 0.606 ± 0.236
1.125TrpSer: 1.125 ± 0.277
0.606TrpThr: 0.606 ± 0.221
1.125TrpVal: 1.125 ± 0.296
0.26TrpTrp: 0.26 ± 0.141
0.346TrpTyr: 0.346 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.818TyrAla: 1.818 ± 0.401
0.173TyrCys: 0.173 ± 0.113
2.943TyrAsp: 2.943 ± 0.558
3.03TyrGlu: 3.03 ± 0.606
2.078TyrPhe: 2.078 ± 0.42
2.337TyrGly: 2.337 ± 0.408
0.433TyrHis: 0.433 ± 0.193
2.77TyrIle: 2.77 ± 0.449
3.463TyrLys: 3.463 ± 0.692
2.857TyrLeu: 2.857 ± 0.445
0.433TyrMet: 0.433 ± 0.191
2.337TyrAsn: 2.337 ± 0.463
0.779TyrPro: 0.779 ± 0.249
1.731TyrGln: 1.731 ± 0.309
1.991TyrArg: 1.991 ± 0.433
2.943TyrSer: 2.943 ± 0.5
1.905TyrThr: 1.905 ± 0.459
1.299TyrVal: 1.299 ± 0.287
0.606TyrTrp: 0.606 ± 0.233
1.299TyrTyr: 1.299 ± 0.351
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (11552 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski