Candidatus Thiomargarita nelsonii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria<

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5897 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4E0QWB0|A0A4E0QWB0_9GAMM Uncharacterized protein OS=Candidatus Thiomargarita nelsonii OX=1003181 GN=PN36_09985 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84SNIKK6 pKa = 10.29KK7 pKa = 8.37QTLEE11 pKa = 3.88HH12 pKa = 7.04AICGPQTKK20 pKa = 9.11PWRR23 pKa = 11.84SSLASSLNSLCPSSNWRR40 pKa = 11.84GVVFVAFIVGIMAIFASTQVQAVPIPGVSEE70 pKa = 4.14YY71 pKa = 10.75FAGTVTDD78 pKa = 3.7QNGDD82 pKa = 4.14PISDD86 pKa = 3.25ATVQINEE93 pKa = 4.17VADD96 pKa = 3.4NTEE99 pKa = 3.91RR100 pKa = 11.84DD101 pKa = 3.68GSFGFHH107 pKa = 6.58VEE109 pKa = 5.52RR110 pKa = 11.84DD111 pKa = 3.73DD112 pKa = 3.64QDD114 pKa = 3.52QYY116 pKa = 11.42IINVTKK122 pKa = 10.42RR123 pKa = 11.84GYY125 pKa = 11.18ALVSQIEE132 pKa = 4.16HH133 pKa = 6.72APNVSLRR140 pKa = 11.84FTLKK144 pKa = 9.49QAEE147 pKa = 4.38VFIIDD152 pKa = 3.89PSQTSKK158 pKa = 11.28VEE160 pKa = 4.02DD161 pKa = 3.45SRR163 pKa = 11.84GTRR166 pKa = 11.84IMLPPNSLVDD176 pKa = 3.81SDD178 pKa = 4.96GNPPDD183 pKa = 5.17GEE185 pKa = 4.42VQMGLYY191 pKa = 9.85TYY193 pKa = 11.14DD194 pKa = 3.86LANEE198 pKa = 4.17SMPGDD203 pKa = 3.3MGAINSQGEE212 pKa = 4.2PGAMLSAGAFYY223 pKa = 11.26AEE225 pKa = 4.34FTDD228 pKa = 4.82DD229 pKa = 3.41SGRR232 pKa = 11.84MYY234 pKa = 11.21DD235 pKa = 3.77LASDD239 pKa = 4.03MVAEE243 pKa = 4.38ISIPAINSEE252 pKa = 4.28EE253 pKa = 4.33TVGLWDD259 pKa = 3.72YY260 pKa = 11.67NKK262 pKa = 9.01EE263 pKa = 4.11TGKK266 pKa = 10.03WMEE269 pKa = 5.29DD270 pKa = 3.21GSASLINGRR279 pKa = 11.84FEE281 pKa = 4.04GTVKK285 pKa = 10.53HH286 pKa = 5.55FSVWNFDD293 pKa = 3.31LFYY296 pKa = 11.42NGVACVKK303 pKa = 10.81LEE305 pKa = 3.94IAQAFYY311 pKa = 9.4DD312 pKa = 4.05TYY314 pKa = 11.5KK315 pKa = 11.03DD316 pKa = 3.44GAGVLKK322 pKa = 10.27IQAVVTTPGFSPITDD337 pKa = 3.64VLSMDD342 pKa = 4.25EE343 pKa = 4.21YY344 pKa = 11.49NYY346 pKa = 10.81DD347 pKa = 4.47DD348 pKa = 5.75PYY350 pKa = 11.92ALYY353 pKa = 10.32NLPPNATVDD362 pKa = 4.27FYY364 pKa = 11.89VPPPFSLPPYY374 pKa = 9.53ASVSTGAAWGGTGFPPHH391 pKa = 7.27PYY393 pKa = 9.69DD394 pKa = 3.34VCNGSMVLNFPFSNDD409 pKa = 3.17VIIDD413 pKa = 4.12FGTQNGILVWMNNSAWVQLHH433 pKa = 6.21PLSPDD438 pKa = 3.08SMVTGDD444 pKa = 3.94MDD446 pKa = 5.5GNGQDD451 pKa = 4.85DD452 pKa = 4.43VIIDD456 pKa = 4.14FGSPHH461 pKa = 7.41GILVWMNNSAWVQLHH476 pKa = 6.19PLSPSSMITGDD487 pKa = 3.17MDD489 pKa = 5.08GNGLDD494 pKa = 4.55DD495 pKa = 5.19VIIDD499 pKa = 3.71FPGYY503 pKa = 9.94GIWVMMNNSTWVQLHH518 pKa = 5.78SLSPSSMTTGDD529 pKa = 3.18MDD531 pKa = 5.13GNGLDD536 pKa = 4.55DD537 pKa = 5.19VIIDD541 pKa = 3.71FPGYY545 pKa = 9.94GIWVMMNNSTWVQLHH560 pKa = 5.78SLSPSSMTTGDD571 pKa = 3.18MDD573 pKa = 5.13GNGLDD578 pKa = 4.55DD579 pKa = 5.19VIIDD583 pKa = 3.71FPGYY587 pKa = 9.94GIWVMMNNSTWVQLHH602 pKa = 5.78SLSPSSMTTGDD613 pKa = 3.18MDD615 pKa = 5.13GNGLDD620 pKa = 4.55DD621 pKa = 5.19VIIDD625 pKa = 3.71FPGYY629 pKa = 9.94GIWVMMNNSTWVNLHH644 pKa = 5.93SLSPSSMTTGDD655 pKa = 4.14LDD657 pKa = 3.97NNAQADD663 pKa = 4.13VIIDD667 pKa = 3.81FGPGVGILVMMNNSTWVTLHH687 pKa = 6.53SNSADD692 pKa = 3.3SMVTGNIDD700 pKa = 3.33GLPP703 pKa = 3.44

Molecular weight: 76.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4E0QLU1|A0A4E0QLU1_9GAMM Uncharacterized protein OS=Candidatus Thiomargarita nelsonii OX=1003181 GN=PN36_35000 PE=4 SV=1
MM1 pKa = 7.32HH2 pKa = 7.64RR3 pKa = 11.84ALSLKK8 pKa = 10.29NFSLFLLRR16 pKa = 11.84GLLTGNTLRR25 pKa = 11.84LAQTIIWRR33 pKa = 11.84TKK35 pKa = 9.33KK36 pKa = 10.54KK37 pKa = 10.48SIRR40 pKa = 11.84PQINMKK46 pKa = 10.34RR47 pKa = 11.84LIKK50 pKa = 10.23PVVKK54 pKa = 9.37PVLMIQVLAVFQQMM68 pKa = 3.65

Molecular weight: 7.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5897

0

5897

1621432

51

4061

275.0

31.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.362 ± 0.038

1.07 ± 0.012

5.272 ± 0.026

6.474 ± 0.036

4.366 ± 0.024

6.272 ± 0.037

2.257 ± 0.017

6.997 ± 0.032

6.242 ± 0.041

10.677 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.198 ± 0.017

4.564 ± 0.027

4.199 ± 0.022

4.701 ± 0.033

4.898 ± 0.026

6.066 ± 0.029

5.417 ± 0.033

6.051 ± 0.028

1.471 ± 0.014

3.447 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski