Amino acid dipepetide frequency for Moraxella phage Mcat25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.641AlaAla: 4.641 ± 0.986
1.384AlaCys: 1.384 ± 0.351
4.803AlaAsp: 4.803 ± 0.715
4.478AlaGlu: 4.478 ± 0.548
3.012AlaPhe: 3.012 ± 0.553
6.187AlaGly: 6.187 ± 1.138
2.198AlaHis: 2.198 ± 0.374
5.943AlaIle: 5.943 ± 0.654
6.432AlaLys: 6.432 ± 1.245
6.92AlaLeu: 6.92 ± 0.882
2.035AlaMet: 2.035 ± 0.594
5.292AlaAsn: 5.292 ± 1.072
2.28AlaPro: 2.28 ± 0.618
3.826AlaGln: 3.826 ± 0.68
3.338AlaArg: 3.338 ± 0.598
7.164AlaSer: 7.164 ± 0.701
6.106AlaThr: 6.106 ± 0.883
5.292AlaVal: 5.292 ± 0.715
1.14AlaTrp: 1.14 ± 0.298
2.687AlaTyr: 2.687 ± 0.511
0.0AlaXaa: 0.0 ± 0.0
Cys
0.651CysAla: 0.651 ± 0.256
0.163CysCys: 0.163 ± 0.102
0.326CysAsp: 0.326 ± 0.178
0.733CysGlu: 0.733 ± 0.315
0.244CysPhe: 0.244 ± 0.133
0.896CysGly: 0.896 ± 0.314
0.326CysHis: 0.326 ± 0.193
0.407CysIle: 0.407 ± 0.173
0.896CysLys: 0.896 ± 0.334
0.814CysLeu: 0.814 ± 0.282
0.326CysMet: 0.326 ± 0.199
0.488CysAsn: 0.488 ± 0.19
0.326CysPro: 0.326 ± 0.168
0.57CysGln: 0.57 ± 0.196
0.733CysArg: 0.733 ± 0.293
0.977CysSer: 0.977 ± 0.287
0.244CysThr: 0.244 ± 0.159
0.733CysVal: 0.733 ± 0.287
0.163CysTrp: 0.163 ± 0.118
0.488CysTyr: 0.488 ± 0.222
0.0CysXaa: 0.0 ± 0.0
Asp
4.641AspAla: 4.641 ± 0.643
0.326AspCys: 0.326 ± 0.158
4.885AspAsp: 4.885 ± 0.881
5.048AspGlu: 5.048 ± 0.681
3.257AspPhe: 3.257 ± 0.644
5.618AspGly: 5.618 ± 0.681
0.896AspHis: 0.896 ± 0.296
4.315AspIle: 4.315 ± 0.547
4.885AspLys: 4.885 ± 0.628
4.722AspLeu: 4.722 ± 0.532
1.547AspMet: 1.547 ± 0.396
3.257AspAsn: 3.257 ± 0.4
1.384AspPro: 1.384 ± 0.381
1.14AspGln: 1.14 ± 0.337
2.117AspArg: 2.117 ± 0.372
3.745AspSer: 3.745 ± 0.552
3.012AspThr: 3.012 ± 0.459
3.745AspVal: 3.745 ± 0.53
0.814AspTrp: 0.814 ± 0.245
2.198AspTyr: 2.198 ± 0.499
0.0AspXaa: 0.0 ± 0.0
Glu
4.152GluAla: 4.152 ± 0.574
0.896GluCys: 0.896 ± 0.296
2.687GluAsp: 2.687 ± 0.505
2.035GluGlu: 2.035 ± 0.503
2.768GluPhe: 2.768 ± 0.57
2.524GluGly: 2.524 ± 0.334
1.547GluHis: 1.547 ± 0.492
4.396GluIle: 4.396 ± 0.77
3.582GluLys: 3.582 ± 0.613
7.164GluLeu: 7.164 ± 0.813
2.28GluMet: 2.28 ± 0.575
2.361GluAsn: 2.361 ± 0.497
1.791GluPro: 1.791 ± 0.532
3.012GluGln: 3.012 ± 0.716
3.175GluArg: 3.175 ± 0.639
2.198GluSer: 2.198 ± 0.521
2.605GluThr: 2.605 ± 0.426
3.664GluVal: 3.664 ± 0.645
0.977GluTrp: 0.977 ± 0.247
2.361GluTyr: 2.361 ± 0.386
0.0GluXaa: 0.0 ± 0.0
Phe
2.442PheAla: 2.442 ± 0.554
0.896PheCys: 0.896 ± 0.266
3.175PheAsp: 3.175 ± 0.466
3.338PheGlu: 3.338 ± 0.555
0.977PhePhe: 0.977 ± 0.346
2.931PheGly: 2.931 ± 0.588
0.733PheHis: 0.733 ± 0.258
2.605PheIle: 2.605 ± 0.436
1.14PheLys: 1.14 ± 0.315
2.442PheLeu: 2.442 ± 0.584
0.814PheMet: 0.814 ± 0.249
1.465PheAsn: 1.465 ± 0.346
0.896PhePro: 0.896 ± 0.273
0.733PheGln: 0.733 ± 0.258
1.14PheArg: 1.14 ± 0.361
2.28PheSer: 2.28 ± 0.35
2.198PheThr: 2.198 ± 0.357
2.361PheVal: 2.361 ± 0.636
0.488PheTrp: 0.488 ± 0.207
1.058PheTyr: 1.058 ± 0.274
0.0PheXaa: 0.0 ± 0.0
Gly
4.478GlyAla: 4.478 ± 0.807
0.488GlyCys: 0.488 ± 0.175
3.826GlyAsp: 3.826 ± 0.611
4.478GlyGlu: 4.478 ± 0.676
2.931GlyPhe: 2.931 ± 0.454
4.641GlyGly: 4.641 ± 1.066
1.221GlyHis: 1.221 ± 0.334
4.152GlyIle: 4.152 ± 0.728
5.78GlyLys: 5.78 ± 0.666
6.187GlyLeu: 6.187 ± 0.542
2.035GlyMet: 2.035 ± 0.4
4.071GlyAsn: 4.071 ± 1.014
0.326GlyPro: 0.326 ± 0.175
2.768GlyGln: 2.768 ± 0.58
3.664GlyArg: 3.664 ± 0.518
4.885GlySer: 4.885 ± 0.896
3.419GlyThr: 3.419 ± 0.804
5.455GlyVal: 5.455 ± 0.707
0.57GlyTrp: 0.57 ± 0.247
1.791GlyTyr: 1.791 ± 0.555
0.0GlyXaa: 0.0 ± 0.0
His
2.361HisAla: 2.361 ± 0.453
0.407HisCys: 0.407 ± 0.203
1.628HisAsp: 1.628 ± 0.389
1.303HisGlu: 1.303 ± 0.336
0.896HisPhe: 0.896 ± 0.227
1.791HisGly: 1.791 ± 0.421
1.14HisHis: 1.14 ± 0.383
1.221HisIle: 1.221 ± 0.295
0.977HisLys: 0.977 ± 0.238
2.849HisLeu: 2.849 ± 0.429
0.651HisMet: 0.651 ± 0.304
1.303HisAsn: 1.303 ± 0.324
0.733HisPro: 0.733 ± 0.22
0.814HisGln: 0.814 ± 0.228
1.058HisArg: 1.058 ± 0.304
1.628HisSer: 1.628 ± 0.358
2.361HisThr: 2.361 ± 0.51
0.57HisVal: 0.57 ± 0.239
0.326HisTrp: 0.326 ± 0.171
0.977HisTyr: 0.977 ± 0.35
0.0HisXaa: 0.0 ± 0.0
Ile
5.536IleAla: 5.536 ± 0.694
0.57IleCys: 0.57 ± 0.237
5.048IleAsp: 5.048 ± 0.681
3.989IleGlu: 3.989 ± 0.611
1.791IlePhe: 1.791 ± 0.442
4.478IleGly: 4.478 ± 0.492
1.303IleHis: 1.303 ± 0.235
4.152IleIle: 4.152 ± 0.649
6.106IleLys: 6.106 ± 0.859
4.396IleLeu: 4.396 ± 0.648
1.465IleMet: 1.465 ± 0.369
3.745IleAsn: 3.745 ± 0.529
1.791IlePro: 1.791 ± 0.47
2.605IleGln: 2.605 ± 0.604
2.361IleArg: 2.361 ± 0.375
4.885IleSer: 4.885 ± 0.749
6.025IleThr: 6.025 ± 1.03
2.198IleVal: 2.198 ± 0.414
0.244IleTrp: 0.244 ± 0.126
2.524IleTyr: 2.524 ± 0.503
0.0IleXaa: 0.0 ± 0.0
Lys
6.757LysAla: 6.757 ± 0.72
0.326LysCys: 0.326 ± 0.164
4.152LysAsp: 4.152 ± 0.62
2.442LysGlu: 2.442 ± 0.736
1.791LysPhe: 1.791 ± 0.408
3.908LysGly: 3.908 ± 0.562
1.221LysHis: 1.221 ± 0.362
4.559LysIle: 4.559 ± 0.542
4.966LysLys: 4.966 ± 0.829
5.699LysLeu: 5.699 ± 0.66
1.221LysMet: 1.221 ± 0.303
4.641LysAsn: 4.641 ± 0.62
3.257LysPro: 3.257 ± 0.731
3.826LysGln: 3.826 ± 0.574
2.849LysArg: 2.849 ± 0.416
5.455LysSer: 5.455 ± 0.695
4.233LysThr: 4.233 ± 0.646
2.768LysVal: 2.768 ± 0.505
1.058LysTrp: 1.058 ± 0.292
2.035LysTyr: 2.035 ± 0.401
0.0LysXaa: 0.0 ± 0.0
Leu
8.793LeuAla: 8.793 ± 1.462
0.814LeuCys: 0.814 ± 0.331
5.943LeuAsp: 5.943 ± 0.696
5.455LeuGlu: 5.455 ± 0.676
2.035LeuPhe: 2.035 ± 0.463
7.083LeuGly: 7.083 ± 1.156
2.198LeuHis: 2.198 ± 0.564
4.641LeuIle: 4.641 ± 0.521
5.455LeuLys: 5.455 ± 0.584
7.49LeuLeu: 7.49 ± 1.012
1.465LeuMet: 1.465 ± 0.405
4.559LeuAsn: 4.559 ± 0.975
3.257LeuPro: 3.257 ± 0.647
4.315LeuGln: 4.315 ± 0.383
3.826LeuArg: 3.826 ± 0.582
6.757LeuSer: 6.757 ± 0.658
6.025LeuThr: 6.025 ± 0.888
4.478LeuVal: 4.478 ± 0.737
0.977LeuTrp: 0.977 ± 0.266
2.768LeuTyr: 2.768 ± 0.43
0.0LeuXaa: 0.0 ± 0.0
Met
2.687MetAla: 2.687 ± 0.684
0.163MetCys: 0.163 ± 0.127
1.465MetAsp: 1.465 ± 0.315
0.977MetGlu: 0.977 ± 0.329
1.058MetPhe: 1.058 ± 0.311
1.303MetGly: 1.303 ± 0.344
0.651MetHis: 0.651 ± 0.285
1.384MetIle: 1.384 ± 0.493
0.977MetLys: 0.977 ± 0.317
1.628MetLeu: 1.628 ± 0.505
0.896MetMet: 0.896 ± 0.289
1.628MetAsn: 1.628 ± 0.439
0.896MetPro: 0.896 ± 0.257
1.384MetGln: 1.384 ± 0.483
1.384MetArg: 1.384 ± 0.357
1.954MetSer: 1.954 ± 0.494
2.28MetThr: 2.28 ± 0.397
1.547MetVal: 1.547 ± 0.6
0.163MetTrp: 0.163 ± 0.11
0.488MetTyr: 0.488 ± 0.244
0.0MetXaa: 0.0 ± 0.0
Asn
4.966AsnAla: 4.966 ± 0.782
0.407AsnCys: 0.407 ± 0.214
3.419AsnAsp: 3.419 ± 0.609
2.198AsnGlu: 2.198 ± 0.345
2.035AsnPhe: 2.035 ± 0.46
3.257AsnGly: 3.257 ± 0.726
2.035AsnHis: 2.035 ± 0.524
3.501AsnIle: 3.501 ± 0.772
3.664AsnLys: 3.664 ± 0.794
5.373AsnLeu: 5.373 ± 0.758
1.14AsnMet: 1.14 ± 0.385
3.094AsnAsn: 3.094 ± 0.656
2.442AsnPro: 2.442 ± 0.477
3.664AsnGln: 3.664 ± 0.843
2.198AsnArg: 2.198 ± 0.476
4.315AsnSer: 4.315 ± 0.949
3.419AsnThr: 3.419 ± 0.602
2.687AsnVal: 2.687 ± 0.441
0.244AsnTrp: 0.244 ± 0.125
1.628AsnTyr: 1.628 ± 0.459
0.0AsnXaa: 0.0 ± 0.0
Pro
2.605ProAla: 2.605 ± 0.532
0.651ProCys: 0.651 ± 0.232
1.71ProAsp: 1.71 ± 0.376
1.628ProGlu: 1.628 ± 0.459
1.71ProPhe: 1.71 ± 0.34
0.488ProGly: 0.488 ± 0.183
0.57ProHis: 0.57 ± 0.228
3.175ProIle: 3.175 ± 0.417
1.791ProLys: 1.791 ± 0.424
1.954ProLeu: 1.954 ± 0.419
0.977ProMet: 0.977 ± 0.235
2.442ProAsn: 2.442 ± 0.517
1.14ProPro: 1.14 ± 0.376
1.058ProGln: 1.058 ± 0.327
0.814ProArg: 0.814 ± 0.233
3.338ProSer: 3.338 ± 0.652
2.442ProThr: 2.442 ± 0.405
2.198ProVal: 2.198 ± 0.448
0.326ProTrp: 0.326 ± 0.16
0.488ProTyr: 0.488 ± 0.148
0.0ProXaa: 0.0 ± 0.0
Gln
5.129GlnAla: 5.129 ± 0.721
0.326GlnCys: 0.326 ± 0.196
2.361GlnAsp: 2.361 ± 0.483
2.687GlnGlu: 2.687 ± 0.605
1.384GlnPhe: 1.384 ± 0.37
3.745GlnGly: 3.745 ± 1.485
0.733GlnHis: 0.733 ± 0.207
3.501GlnIle: 3.501 ± 0.568
3.582GlnLys: 3.582 ± 0.401
3.419GlnLeu: 3.419 ± 0.434
1.058GlnMet: 1.058 ± 0.271
2.28GlnAsn: 2.28 ± 0.511
1.14GlnPro: 1.14 ± 0.283
2.035GlnGln: 2.035 ± 0.545
2.117GlnArg: 2.117 ± 0.525
2.849GlnSer: 2.849 ± 0.488
4.152GlnThr: 4.152 ± 0.886
2.442GlnVal: 2.442 ± 0.5
0.326GlnTrp: 0.326 ± 0.173
1.954GlnTyr: 1.954 ± 0.391
0.0GlnXaa: 0.0 ± 0.0
Arg
4.233ArgAla: 4.233 ± 0.58
0.57ArgCys: 0.57 ± 0.189
2.117ArgAsp: 2.117 ± 0.388
2.849ArgGlu: 2.849 ± 0.587
2.035ArgPhe: 2.035 ± 0.46
1.628ArgGly: 1.628 ± 0.383
1.465ArgHis: 1.465 ± 0.361
2.768ArgIle: 2.768 ± 0.531
2.198ArgLys: 2.198 ± 0.411
5.048ArgLeu: 5.048 ± 0.815
0.977ArgMet: 0.977 ± 0.376
1.873ArgAsn: 1.873 ± 0.376
1.058ArgPro: 1.058 ± 0.296
2.524ArgGln: 2.524 ± 0.419
1.791ArgArg: 1.791 ± 0.418
2.605ArgSer: 2.605 ± 0.47
2.849ArgThr: 2.849 ± 0.472
1.791ArgVal: 1.791 ± 0.362
0.896ArgTrp: 0.896 ± 0.236
2.361ArgTyr: 2.361 ± 0.461
0.0ArgXaa: 0.0 ± 0.0
Ser
5.129SerAla: 5.129 ± 0.7
0.326SerCys: 0.326 ± 0.161
3.745SerAsp: 3.745 ± 0.615
4.559SerGlu: 4.559 ± 0.563
2.442SerPhe: 2.442 ± 0.526
4.315SerGly: 4.315 ± 0.481
2.28SerHis: 2.28 ± 0.655
4.396SerIle: 4.396 ± 0.655
4.803SerLys: 4.803 ± 0.596
7.409SerLeu: 7.409 ± 0.962
1.058SerMet: 1.058 ± 0.288
4.722SerAsn: 4.722 ± 0.793
2.117SerPro: 2.117 ± 0.532
3.338SerGln: 3.338 ± 0.751
3.175SerArg: 3.175 ± 0.467
4.233SerSer: 4.233 ± 0.988
4.152SerThr: 4.152 ± 0.554
4.722SerVal: 4.722 ± 0.784
0.651SerTrp: 0.651 ± 0.327
1.628SerTyr: 1.628 ± 0.387
0.0SerXaa: 0.0 ± 0.0
Thr
7.897ThrAla: 7.897 ± 1.319
0.244ThrCys: 0.244 ± 0.157
3.989ThrAsp: 3.989 ± 0.543
3.175ThrGlu: 3.175 ± 0.677
1.14ThrPhe: 1.14 ± 0.248
5.048ThrGly: 5.048 ± 0.76
1.628ThrHis: 1.628 ± 0.359
3.745ThrIle: 3.745 ± 0.512
4.478ThrLys: 4.478 ± 0.714
6.594ThrLeu: 6.594 ± 1.119
1.71ThrMet: 1.71 ± 0.351
4.071ThrAsn: 4.071 ± 0.988
3.419ThrPro: 3.419 ± 0.561
3.094ThrGln: 3.094 ± 0.618
2.035ThrArg: 2.035 ± 0.412
3.257ThrSer: 3.257 ± 0.69
5.129ThrThr: 5.129 ± 1.636
3.501ThrVal: 3.501 ± 0.439
0.814ThrTrp: 0.814 ± 0.195
1.628ThrTyr: 1.628 ± 0.285
0.0ThrXaa: 0.0 ± 0.0
Val
4.559ValAla: 4.559 ± 0.753
0.977ValCys: 0.977 ± 0.343
3.257ValAsp: 3.257 ± 0.562
2.687ValGlu: 2.687 ± 0.519
1.303ValPhe: 1.303 ± 0.319
3.664ValGly: 3.664 ± 0.682
1.303ValHis: 1.303 ± 0.344
3.664ValIle: 3.664 ± 0.591
3.094ValLys: 3.094 ± 0.493
4.803ValLeu: 4.803 ± 0.847
1.873ValMet: 1.873 ± 0.373
2.931ValAsn: 2.931 ± 0.421
1.628ValPro: 1.628 ± 0.318
3.338ValGln: 3.338 ± 0.372
2.687ValArg: 2.687 ± 0.579
3.501ValSer: 3.501 ± 0.407
3.989ValThr: 3.989 ± 0.557
3.419ValVal: 3.419 ± 0.542
1.14ValTrp: 1.14 ± 0.278
2.198ValTyr: 2.198 ± 0.428
0.0ValXaa: 0.0 ± 0.0
Trp
0.733TrpAla: 0.733 ± 0.26
0.244TrpCys: 0.244 ± 0.138
1.058TrpAsp: 1.058 ± 0.233
0.57TrpGlu: 0.57 ± 0.221
0.326TrpPhe: 0.326 ± 0.175
0.733TrpGly: 0.733 ± 0.234
0.163TrpHis: 0.163 ± 0.126
0.407TrpIle: 0.407 ± 0.172
0.488TrpLys: 0.488 ± 0.168
0.977TrpLeu: 0.977 ± 0.304
0.326TrpMet: 0.326 ± 0.178
0.326TrpAsn: 0.326 ± 0.201
0.244TrpPro: 0.244 ± 0.133
1.221TrpGln: 1.221 ± 0.291
0.896TrpArg: 0.896 ± 0.288
0.814TrpSer: 0.814 ± 0.29
0.407TrpThr: 0.407 ± 0.157
0.814TrpVal: 0.814 ± 0.188
0.163TrpTrp: 0.163 ± 0.113
0.733TrpTyr: 0.733 ± 0.235
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.768TyrAla: 2.768 ± 0.601
0.326TyrCys: 0.326 ± 0.202
1.954TyrAsp: 1.954 ± 0.36
1.547TyrGlu: 1.547 ± 0.393
0.977TyrPhe: 0.977 ± 0.338
2.931TyrGly: 2.931 ± 0.53
1.384TyrHis: 1.384 ± 0.315
2.28TyrIle: 2.28 ± 0.44
1.873TyrLys: 1.873 ± 0.433
2.442TyrLeu: 2.442 ± 0.406
0.977TyrMet: 0.977 ± 0.325
1.14TyrAsn: 1.14 ± 0.352
1.384TyrPro: 1.384 ± 0.393
1.954TyrGln: 1.954 ± 0.368
2.28TyrArg: 2.28 ± 0.387
2.361TyrSer: 2.361 ± 0.485
1.547TyrThr: 1.547 ± 0.368
1.71TyrVal: 1.71 ± 0.401
0.163TyrTrp: 0.163 ± 0.118
1.221TyrTyr: 1.221 ± 0.393
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (12284 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski