Helicobasidium mompa totivirus 1-17

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; Victorivirus

Average proteome isoelectric point is 8.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q76L28|Q76L28_9VIRU Coat protein OS=Helicobasidium mompa totivirus 1-17 OX=196690 GN=CP PE=4 SV=1
MM1 pKa = 7.59SSTSEE6 pKa = 4.25TNQVNYY12 pKa = 10.19LAGVIANPRR21 pKa = 11.84GGQMGQQYY29 pKa = 10.09RR30 pKa = 11.84STVSTITTMAKK41 pKa = 10.01VNSNADD47 pKa = 3.3SRR49 pKa = 11.84SSVVKK54 pKa = 9.09YY55 pKa = 10.26EE56 pKa = 3.22IGRR59 pKa = 11.84RR60 pKa = 11.84HH61 pKa = 6.19DD62 pKa = 3.21ARR64 pKa = 11.84RR65 pKa = 11.84NAFAPYY71 pKa = 9.12ADD73 pKa = 3.43EE74 pKa = 5.31AIRR77 pKa = 11.84VDD79 pKa = 3.59VSYY82 pKa = 8.11DD83 pKa = 3.17TPAIMSEE90 pKa = 4.29TFAGLAKK97 pKa = 10.24KK98 pKa = 10.36YY99 pKa = 10.48SNFSASFEE107 pKa = 4.13RR108 pKa = 11.84SSLAGIVEE116 pKa = 4.46RR117 pKa = 11.84LAKK120 pKa = 10.33VSRR123 pKa = 11.84CKK125 pKa = 10.51LLPRR129 pKa = 11.84CYY131 pKa = 9.85IGGFDD136 pKa = 3.64RR137 pKa = 11.84RR138 pKa = 11.84ARR140 pKa = 11.84DD141 pKa = 3.57PVTGLATHH149 pKa = 6.55FTPVSSLDD157 pKa = 3.22ASVFIPRR164 pKa = 11.84RR165 pKa = 11.84IDD167 pKa = 2.96TMTCPGVFAVLCAAVAGEE185 pKa = 4.62GSQIVTDD192 pKa = 4.87LIEE195 pKa = 4.63INVGNNHH202 pKa = 7.2PIVTTVDD209 pKa = 3.53VAGLPAACVGALRR222 pKa = 11.84VLGANMIAAGQGDD235 pKa = 4.46LFGYY239 pKa = 10.25AVARR243 pKa = 11.84GIHH246 pKa = 4.73QVVSVVGHH254 pKa = 5.13TDD256 pKa = 2.72EE257 pKa = 5.11GGVMRR262 pKa = 11.84DD263 pKa = 3.5LLRR266 pKa = 11.84VDD268 pKa = 4.4GFGAPFGGIHH278 pKa = 6.78FGLPVYY284 pKa = 10.1TGLPHH289 pKa = 7.61IDD291 pKa = 3.45VGNDD295 pKa = 3.19LQLSGWVDD303 pKa = 4.95GIALSTAALVAHH315 pKa = 7.25CDD317 pKa = 3.07HH318 pKa = 6.23GVVYY322 pKa = 10.75NGTWFPTTLSGSAAAPVEE340 pKa = 4.4PGLDD344 pKa = 3.25APAGDD349 pKa = 3.8EE350 pKa = 4.67GYY352 pKa = 10.64VARR355 pKa = 11.84NRR357 pKa = 11.84NQLADD362 pKa = 3.81EE363 pKa = 4.98LSPFSLNYY371 pKa = 10.79ANGLAKK377 pKa = 10.76LFGAGTAGGLTVLHH391 pKa = 6.49LSGRR395 pKa = 11.84AGSLADD401 pKa = 4.11DD402 pKa = 3.79CRR404 pKa = 11.84HH405 pKa = 6.48LNFATANPFFWIEE418 pKa = 4.09STSILPANFLGTQAEE433 pKa = 4.67TEE435 pKa = 4.18GRR437 pKa = 11.84ASYY440 pKa = 10.71GGRR443 pKa = 11.84CSNSSLPLWNQATCVSAVDD462 pKa = 3.92SAVCEE467 pKa = 4.35YY468 pKa = 9.01TVEE471 pKa = 4.13MASPRR476 pKa = 11.84RR477 pKa = 11.84VPFMLHH483 pKa = 5.23WNGNPRR489 pKa = 11.84NGLGNILPRR498 pKa = 11.84QMDD501 pKa = 4.05PMAIIQPGGAEE512 pKa = 3.48ARR514 pKa = 11.84SIRR517 pKa = 11.84EE518 pKa = 3.53RR519 pKa = 11.84MIANNDD525 pKa = 3.37LASYY529 pKa = 10.19LWTRR533 pKa = 11.84GQSPIAAPGEE543 pKa = 4.03FMNTSINVGILVKK556 pKa = 10.49HH557 pKa = 6.77CEE559 pKa = 4.4TTWDD563 pKa = 3.87GAVLSHH569 pKa = 6.75LPSPSEE575 pKa = 4.01FTTGALWLQVGRR587 pKa = 11.84PTGVAVGPLGKK598 pKa = 9.01TNANEE603 pKa = 3.68RR604 pKa = 11.84RR605 pKa = 11.84ARR607 pKa = 11.84TAATNALLRR616 pKa = 11.84ARR618 pKa = 11.84HH619 pKa = 6.12LSRR622 pKa = 11.84GHH624 pKa = 6.07NLVAVNMLPLSFSAPSFGAPRR645 pKa = 11.84VIEE648 pKa = 3.85THH650 pKa = 7.03DD651 pKa = 4.27NINRR655 pKa = 11.84APPGGGSADD664 pKa = 3.3TGVGVDD670 pKa = 3.51RR671 pKa = 11.84VVNAADD677 pKa = 3.34AQGPLRR683 pKa = 11.84PTGRR687 pKa = 11.84TTSNGCPSRR696 pKa = 11.84QGDD699 pKa = 3.59QTWRR703 pKa = 11.84VAVHH707 pKa = 6.47GLSLSPSTLLRR718 pKa = 11.84RR719 pKa = 11.84CLEE722 pKa = 4.31GGCATRR728 pKa = 11.84SVAAACRR735 pKa = 11.84RR736 pKa = 11.84DD737 pKa = 3.52SCTGPRR743 pKa = 11.84NGGGAASGRR752 pKa = 11.84GCRR755 pKa = 11.84TVGPRR760 pKa = 11.84MGPTTFRR767 pKa = 11.84YY768 pKa = 9.68SRR770 pKa = 11.84HH771 pKa = 5.18EE772 pKa = 4.16AEE774 pKa = 6.14AMQQDD779 pKa = 4.05AALQAAGAQQ788 pKa = 3.59

Molecular weight: 82.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q76L27|Q76L27_9VIRU RNA-directed RNA polymerase OS=Helicobasidium mompa totivirus 1-17 OX=196690 GN=RDRP PE=3 SV=1
MM1 pKa = 7.31SRR3 pKa = 11.84NSRR6 pKa = 11.84ITLGLGPNAQKK17 pKa = 10.74AAAPRR22 pKa = 11.84FSRR25 pKa = 11.84SIAVPVRR32 pKa = 11.84APSLVQAYY40 pKa = 9.09NWLRR44 pKa = 11.84NDD46 pKa = 3.62QVQKK50 pKa = 11.3ALALPEE56 pKa = 4.15IEE58 pKa = 4.32PPARR62 pKa = 11.84LLSPPPQPNHH72 pKa = 4.47TT73 pKa = 4.09

Molecular weight: 7.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1706

73

845

568.7

61.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.489 ± 0.74

2.286 ± 0.572

4.631 ± 0.816

4.162 ± 0.622

2.931 ± 0.395

8.558 ± 2.035

2.462 ± 0.271

4.22 ± 0.274

2.989 ± 1.215

9.379 ± 1.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.638 ± 0.528

4.865 ± 0.516

5.217 ± 2.454

3.048 ± 0.922

7.386 ± 0.572

7.268 ± 0.34

5.686 ± 0.882

7.268 ± 0.506

1.348 ± 0.134

2.169 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski