Leptosphaeria biglobosa mitovirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Howeltoviricetes; Cryppavirales; Mitoviridae; Mitovirus; unclassified Mitovirus

Average proteome isoelectric point is 8.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2R4SUF2|A0A2R4SUF2_9VIRU RNA-dependent RNA polymerase OS=Leptosphaeria biglobosa mitovirus 1 OX=2163916 PE=4 SV=1
MM1 pKa = 7.41SLLFKK6 pKa = 10.66ILITMKK12 pKa = 10.39QNNYY16 pKa = 10.3LILEE20 pKa = 4.41RR21 pKa = 11.84LLKK24 pKa = 10.73SLFKK28 pKa = 10.01IDD30 pKa = 3.74CFAIVHH36 pKa = 5.81QYY38 pKa = 11.26LNYY41 pKa = 8.75IDD43 pKa = 5.25RR44 pKa = 11.84MRR46 pKa = 11.84EE47 pKa = 4.05KK48 pKa = 10.86NGLAYY53 pKa = 9.21TIKK56 pKa = 10.38HH57 pKa = 4.93MKK59 pKa = 8.74VVKK62 pKa = 10.05LHH64 pKa = 5.08ITRR67 pKa = 11.84FICNKK72 pKa = 8.95PLKK75 pKa = 10.46SNSNYY80 pKa = 9.93VSLDD84 pKa = 2.75KK85 pKa = 11.03DD86 pKa = 3.75YY87 pKa = 11.08FPSRR91 pKa = 11.84FLYY94 pKa = 10.46LKK96 pKa = 9.88RR97 pKa = 11.84FCKK100 pKa = 10.0TNPDD104 pKa = 3.4LVLTLLSYY112 pKa = 10.33TRR114 pKa = 11.84ALTPNKK120 pKa = 10.2RR121 pKa = 11.84EE122 pKa = 3.95TNARR126 pKa = 11.84NVDD129 pKa = 2.94ISSITNPYY137 pKa = 8.86KK138 pKa = 10.92GKK140 pKa = 10.48DD141 pKa = 3.3YY142 pKa = 10.11TIPIWFIKK150 pKa = 10.54DD151 pKa = 4.08FINHH155 pKa = 6.74HH156 pKa = 6.1GLSLSKK162 pKa = 10.11PVYY165 pKa = 10.45SNSDD169 pKa = 3.0HH170 pKa = 6.18YY171 pKa = 11.57LSIKK175 pKa = 10.22GSPNGKK181 pKa = 9.87SSISSLWSVASHH193 pKa = 6.7NEE195 pKa = 3.45NTLEE199 pKa = 4.19YY200 pKa = 9.92IRR202 pKa = 11.84TITGEE207 pKa = 3.92FFKK210 pKa = 10.77EE211 pKa = 3.35ISNYY215 pKa = 5.58YY216 pKa = 8.51TKK218 pKa = 10.44IVLDD222 pKa = 4.01YY223 pKa = 11.53SHH225 pKa = 7.4LIDD228 pKa = 4.03NNKK231 pKa = 9.95KK232 pKa = 9.56ILGKK236 pKa = 10.4LSVVHH241 pKa = 7.19DD242 pKa = 4.35PEE244 pKa = 4.86LKK246 pKa = 9.79EE247 pKa = 4.09RR248 pKa = 11.84VIAMVDD254 pKa = 3.27YY255 pKa = 7.64TTQFTLKK262 pKa = 10.0PIHH265 pKa = 5.96NQLLKK270 pKa = 10.89LLSKK274 pKa = 10.16FDD276 pKa = 3.93CDD278 pKa = 3.34RR279 pKa = 11.84TFTQDD284 pKa = 3.97PFHH287 pKa = 6.72NWQCNSEE294 pKa = 4.28NFHH297 pKa = 7.78SLDD300 pKa = 3.55LSAATDD306 pKa = 3.92RR307 pKa = 11.84FPIDD311 pKa = 3.68LQEE314 pKa = 4.45KK315 pKa = 8.53LLSYY319 pKa = 10.45IYY321 pKa = 10.03DD322 pKa = 3.92DD323 pKa = 4.77KK324 pKa = 11.82DD325 pKa = 3.49FAKK328 pKa = 10.01SWRR331 pKa = 11.84NLLVDD336 pKa = 3.83RR337 pKa = 11.84DD338 pKa = 4.51FIYY341 pKa = 9.8EE342 pKa = 4.07QKK344 pKa = 10.5SIRR347 pKa = 11.84YY348 pKa = 8.98SVGQPMGAYY357 pKa = 8.28TSWAAFTLTHH367 pKa = 6.32HH368 pKa = 7.16LVVQWAAKK376 pKa = 10.32LCGLDD381 pKa = 4.34HH382 pKa = 6.8FNQYY386 pKa = 10.4IILGDD391 pKa = 4.36DD392 pKa = 3.31IVIKK396 pKa = 10.53NNKK399 pKa = 7.97VANKK403 pKa = 9.52YY404 pKa = 7.41ITIMTRR410 pKa = 11.84LGVDD414 pKa = 3.41ISQPKK419 pKa = 7.67THH421 pKa = 6.23VSNDD425 pKa = 3.06TYY427 pKa = 11.38EE428 pKa = 4.02FAKK431 pKa = 10.35RR432 pKa = 11.84WIQKK436 pKa = 9.22GRR438 pKa = 11.84EE439 pKa = 3.81ISGLSLKK446 pKa = 10.9GILNNFKK453 pKa = 10.29NKK455 pKa = 9.71HH456 pKa = 4.16VVYY459 pKa = 10.13MNIFNYY465 pKa = 9.12YY466 pKa = 9.61LRR468 pKa = 11.84RR469 pKa = 11.84PHH471 pKa = 8.32LDD473 pKa = 2.95IDD475 pKa = 4.12LLNLIGNLYY484 pKa = 10.41EE485 pKa = 4.76GIRR488 pKa = 11.84VSNRR492 pKa = 11.84IKK494 pKa = 10.83SKK496 pKa = 9.08NTIIKK501 pKa = 10.03LLYY504 pKa = 10.03DD505 pKa = 3.53FHH507 pKa = 8.45HH508 pKa = 7.19SIRR511 pKa = 11.84FSFNLLSYY519 pKa = 10.93EE520 pKa = 5.41DD521 pKa = 3.2IRR523 pKa = 11.84KK524 pKa = 9.33YY525 pKa = 11.07LNNKK529 pKa = 8.76IVIEE533 pKa = 4.25EE534 pKa = 4.08FNVWPEE540 pKa = 3.5SLIPYY545 pKa = 9.33KK546 pKa = 10.3IRR548 pKa = 11.84EE549 pKa = 4.01ILSYY553 pKa = 11.24GMVPQAEE560 pKa = 4.25SLKK563 pKa = 10.06TNISKK568 pKa = 10.26QMSILRR574 pKa = 11.84EE575 pKa = 4.21SKK577 pKa = 10.58DD578 pKa = 3.23IPIDD582 pKa = 4.32LLVDD586 pKa = 3.72WPLTYY591 pKa = 10.63GFKK594 pKa = 10.7NHH596 pKa = 6.61IEE598 pKa = 4.13SMTNLIEE605 pKa = 4.43GFVNKK610 pKa = 9.68TSQLLDD616 pKa = 4.04IISHH620 pKa = 6.38LRR622 pKa = 11.84LNNLDD627 pKa = 4.49GIMKK631 pKa = 10.22SRR633 pKa = 11.84LNNQDD638 pKa = 4.76LLLLDD643 pKa = 5.01KK644 pKa = 10.6LWKK647 pKa = 10.4DD648 pKa = 3.23SFSRR652 pKa = 11.84YY653 pKa = 9.06ILALKK658 pKa = 8.49TEE660 pKa = 4.33KK661 pKa = 10.95EE662 pKa = 4.24EE663 pKa = 4.11ILNSPEE669 pKa = 5.0DD670 pKa = 3.65KK671 pKa = 10.89PKK673 pKa = 11.21DD674 pKa = 3.68LVSHH678 pKa = 6.37IWSMAWASTPVVYY691 pKa = 8.69KK692 pKa = 10.26TSFVGLKK699 pKa = 10.34SFEE702 pKa = 4.42LYY704 pKa = 10.41LYY706 pKa = 10.74EE707 pKa = 5.91SFDD710 pKa = 4.45GLNTDD715 pKa = 3.74LDD717 pKa = 4.12YY718 pKa = 11.64LRR720 pKa = 11.84LEE722 pKa = 4.26TRR724 pKa = 11.84VDD726 pKa = 3.46EE727 pKa = 4.62SSTNIQDD734 pKa = 4.31RR735 pKa = 11.84IHH737 pKa = 7.03SFVYY741 pKa = 10.51DD742 pKa = 3.61SIKK745 pKa = 10.76NNKK748 pKa = 8.67HH749 pKa = 5.71INWDD753 pKa = 3.51EE754 pKa = 4.24FKK756 pKa = 11.19

Molecular weight:
88.78 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2R4SUF2|A0A2R4SUF2_9VIRU RNA-dependent RNA polymerase OS=Leptosphaeria biglobosa mitovirus 1 OX=2163916 PE=4 SV=1
MM1 pKa = 7.41SLLFKK6 pKa = 10.66ILITMKK12 pKa = 10.39QNNYY16 pKa = 10.3LILEE20 pKa = 4.41RR21 pKa = 11.84LLKK24 pKa = 10.73SLFKK28 pKa = 10.01IDD30 pKa = 3.74CFAIVHH36 pKa = 5.81QYY38 pKa = 11.26LNYY41 pKa = 8.75IDD43 pKa = 5.25RR44 pKa = 11.84MRR46 pKa = 11.84EE47 pKa = 4.05KK48 pKa = 10.86NGLAYY53 pKa = 9.21TIKK56 pKa = 10.38HH57 pKa = 4.93MKK59 pKa = 8.74VVKK62 pKa = 10.05LHH64 pKa = 5.08ITRR67 pKa = 11.84FICNKK72 pKa = 8.95PLKK75 pKa = 10.46SNSNYY80 pKa = 9.93VSLDD84 pKa = 2.75KK85 pKa = 11.03DD86 pKa = 3.75YY87 pKa = 11.08FPSRR91 pKa = 11.84FLYY94 pKa = 10.46LKK96 pKa = 9.88RR97 pKa = 11.84FCKK100 pKa = 10.0TNPDD104 pKa = 3.4LVLTLLSYY112 pKa = 10.33TRR114 pKa = 11.84ALTPNKK120 pKa = 10.2RR121 pKa = 11.84EE122 pKa = 3.95TNARR126 pKa = 11.84NVDD129 pKa = 2.94ISSITNPYY137 pKa = 8.86KK138 pKa = 10.92GKK140 pKa = 10.48DD141 pKa = 3.3YY142 pKa = 10.11TIPIWFIKK150 pKa = 10.54DD151 pKa = 4.08FINHH155 pKa = 6.74HH156 pKa = 6.1GLSLSKK162 pKa = 10.11PVYY165 pKa = 10.45SNSDD169 pKa = 3.0HH170 pKa = 6.18YY171 pKa = 11.57LSIKK175 pKa = 10.22GSPNGKK181 pKa = 9.87SSISSLWSVASHH193 pKa = 6.7NEE195 pKa = 3.45NTLEE199 pKa = 4.19YY200 pKa = 9.92IRR202 pKa = 11.84TITGEE207 pKa = 3.92FFKK210 pKa = 10.77EE211 pKa = 3.35ISNYY215 pKa = 5.58YY216 pKa = 8.51TKK218 pKa = 10.44IVLDD222 pKa = 4.01YY223 pKa = 11.53SHH225 pKa = 7.4LIDD228 pKa = 4.03NNKK231 pKa = 9.95KK232 pKa = 9.56ILGKK236 pKa = 10.4LSVVHH241 pKa = 7.19DD242 pKa = 4.35PEE244 pKa = 4.86LKK246 pKa = 9.79EE247 pKa = 4.09RR248 pKa = 11.84VIAMVDD254 pKa = 3.27YY255 pKa = 7.64TTQFTLKK262 pKa = 10.0PIHH265 pKa = 5.96NQLLKK270 pKa = 10.89LLSKK274 pKa = 10.16FDD276 pKa = 3.93CDD278 pKa = 3.34RR279 pKa = 11.84TFTQDD284 pKa = 3.97PFHH287 pKa = 6.72NWQCNSEE294 pKa = 4.28NFHH297 pKa = 7.78SLDD300 pKa = 3.55LSAATDD306 pKa = 3.92RR307 pKa = 11.84FPIDD311 pKa = 3.68LQEE314 pKa = 4.45KK315 pKa = 8.53LLSYY319 pKa = 10.45IYY321 pKa = 10.03DD322 pKa = 3.92DD323 pKa = 4.77KK324 pKa = 11.82DD325 pKa = 3.49FAKK328 pKa = 10.01SWRR331 pKa = 11.84NLLVDD336 pKa = 3.83RR337 pKa = 11.84DD338 pKa = 4.51FIYY341 pKa = 9.8EE342 pKa = 4.07QKK344 pKa = 10.5SIRR347 pKa = 11.84YY348 pKa = 8.98SVGQPMGAYY357 pKa = 8.28TSWAAFTLTHH367 pKa = 6.32HH368 pKa = 7.16LVVQWAAKK376 pKa = 10.32LCGLDD381 pKa = 4.34HH382 pKa = 6.8FNQYY386 pKa = 10.4IILGDD391 pKa = 4.36DD392 pKa = 3.31IVIKK396 pKa = 10.53NNKK399 pKa = 7.97VANKK403 pKa = 9.52YY404 pKa = 7.41ITIMTRR410 pKa = 11.84LGVDD414 pKa = 3.41ISQPKK419 pKa = 7.67THH421 pKa = 6.23VSNDD425 pKa = 3.06TYY427 pKa = 11.38EE428 pKa = 4.02FAKK431 pKa = 10.35RR432 pKa = 11.84WIQKK436 pKa = 9.22GRR438 pKa = 11.84EE439 pKa = 3.81ISGLSLKK446 pKa = 10.9GILNNFKK453 pKa = 10.29NKK455 pKa = 9.71HH456 pKa = 4.16VVYY459 pKa = 10.13MNIFNYY465 pKa = 9.12YY466 pKa = 9.61LRR468 pKa = 11.84RR469 pKa = 11.84PHH471 pKa = 8.32LDD473 pKa = 2.95IDD475 pKa = 4.12LLNLIGNLYY484 pKa = 10.41EE485 pKa = 4.76GIRR488 pKa = 11.84VSNRR492 pKa = 11.84IKK494 pKa = 10.83SKK496 pKa = 9.08NTIIKK501 pKa = 10.03LLYY504 pKa = 10.03DD505 pKa = 3.53FHH507 pKa = 8.45HH508 pKa = 7.19SIRR511 pKa = 11.84FSFNLLSYY519 pKa = 10.93EE520 pKa = 5.41DD521 pKa = 3.2IRR523 pKa = 11.84KK524 pKa = 9.33YY525 pKa = 11.07LNNKK529 pKa = 8.76IVIEE533 pKa = 4.25EE534 pKa = 4.08FNVWPEE540 pKa = 3.5SLIPYY545 pKa = 9.33KK546 pKa = 10.3IRR548 pKa = 11.84EE549 pKa = 4.01ILSYY553 pKa = 11.24GMVPQAEE560 pKa = 4.25SLKK563 pKa = 10.06TNISKK568 pKa = 10.26QMSILRR574 pKa = 11.84EE575 pKa = 4.21SKK577 pKa = 10.58DD578 pKa = 3.23IPIDD582 pKa = 4.32LLVDD586 pKa = 3.72WPLTYY591 pKa = 10.63GFKK594 pKa = 10.7NHH596 pKa = 6.61IEE598 pKa = 4.13SMTNLIEE605 pKa = 4.43GFVNKK610 pKa = 9.68TSQLLDD616 pKa = 4.04IISHH620 pKa = 6.38LRR622 pKa = 11.84LNNLDD627 pKa = 4.49GIMKK631 pKa = 10.22SRR633 pKa = 11.84LNNQDD638 pKa = 4.76LLLLDD643 pKa = 5.01KK644 pKa = 10.6LWKK647 pKa = 10.4DD648 pKa = 3.23SFSRR652 pKa = 11.84YY653 pKa = 9.06ILALKK658 pKa = 8.49TEE660 pKa = 4.33KK661 pKa = 10.95EE662 pKa = 4.24EE663 pKa = 4.11ILNSPEE669 pKa = 5.0DD670 pKa = 3.65KK671 pKa = 10.89PKK673 pKa = 11.21DD674 pKa = 3.68LVSHH678 pKa = 6.37IWSMAWASTPVVYY691 pKa = 8.69KK692 pKa = 10.26TSFVGLKK699 pKa = 10.34SFEE702 pKa = 4.42LYY704 pKa = 10.41LYY706 pKa = 10.74EE707 pKa = 5.91SFDD710 pKa = 4.45GLNTDD715 pKa = 3.74LDD717 pKa = 4.12YY718 pKa = 11.64LRR720 pKa = 11.84LEE722 pKa = 4.26TRR724 pKa = 11.84VDD726 pKa = 3.46EE727 pKa = 4.62SSTNIQDD734 pKa = 4.31RR735 pKa = 11.84IHH737 pKa = 7.03SFVYY741 pKa = 10.51DD742 pKa = 3.61SIKK745 pKa = 10.76NNKK748 pKa = 8.67HH749 pKa = 5.71INWDD753 pKa = 3.51EE754 pKa = 4.24FKK756 pKa = 11.19

Molecular weight:
88.78 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

756

756

756

756.0

88.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.646 ± 0.0

0.794 ± 0.0

6.614 ± 0.0

4.365 ± 0.0

4.762 ± 0.0

3.042 ± 0.0

3.307 ± 0.0

9.127 ± 0.0

8.73 ± 0.0

12.169 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.72 ± 0.0

7.54 ± 0.0

3.042 ± 0.0

2.381 ± 0.0

4.365 ± 0.0

8.598 ± 0.0

5.026 ± 0.0

4.63 ± 0.0

1.72 ± 0.0

5.423 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski