Amino acid dipepetide frequency for Prochlorococcus marinus (strain MIT 9301)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.011AlaAla: 4.011 ± 0.114
0.752AlaCys: 0.752 ± 0.036
2.473AlaAsp: 2.473 ± 0.075
3.273AlaGlu: 3.273 ± 0.077
2.304AlaPhe: 2.304 ± 0.078
3.805AlaGly: 3.805 ± 0.111
0.85AlaHis: 0.85 ± 0.041
4.974AlaIle: 4.974 ± 0.107
4.144AlaLys: 4.144 ± 0.089
6.1AlaLeu: 6.1 ± 0.137
1.304AlaMet: 1.304 ± 0.055
2.668AlaAsn: 2.668 ± 0.08
1.618AlaPro: 1.618 ± 0.058
1.592AlaGln: 1.592 ± 0.069
2.259AlaArg: 2.259 ± 0.071
4.045AlaSer: 4.045 ± 0.086
2.404AlaThr: 2.404 ± 0.083
3.293AlaVal: 3.293 ± 0.09
0.593AlaTrp: 0.593 ± 0.035
1.554AlaTyr: 1.554 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.536CysAla: 0.536 ± 0.034
0.177CysCys: 0.177 ± 0.019
0.699CysAsp: 0.699 ± 0.038
0.788CysGlu: 0.788 ± 0.04
0.597CysPhe: 0.597 ± 0.036
0.957CysGly: 0.957 ± 0.042
0.226CysHis: 0.226 ± 0.021
0.919CysIle: 0.919 ± 0.044
0.875CysLys: 0.875 ± 0.045
1.203CysLeu: 1.203 ± 0.052
0.189CysMet: 0.189 ± 0.021
0.601CysAsn: 0.601 ± 0.031
0.536CysPro: 0.536 ± 0.034
0.345CysGln: 0.345 ± 0.027
0.484CysArg: 0.484 ± 0.032
0.955CysSer: 0.955 ± 0.044
0.466CysThr: 0.466 ± 0.034
0.601CysVal: 0.601 ± 0.032
0.195CysTrp: 0.195 ± 0.021
0.288CysTyr: 0.288 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
2.505AspAla: 2.505 ± 0.071
0.601AspCys: 0.601 ± 0.035
2.362AspAsp: 2.362 ± 0.065
3.654AspGlu: 3.654 ± 0.073
2.803AspPhe: 2.803 ± 0.084
3.065AspGly: 3.065 ± 0.085
0.81AspHis: 0.81 ± 0.05
4.307AspIle: 4.307 ± 0.095
3.964AspLys: 3.964 ± 0.093
6.804AspLeu: 6.804 ± 0.119
0.846AspMet: 0.846 ± 0.035
3.001AspAsn: 3.001 ± 0.081
2.191AspPro: 2.191 ± 0.072
1.826AspGln: 1.826 ± 0.071
1.933AspArg: 1.933 ± 0.064
3.759AspSer: 3.759 ± 0.087
1.737AspThr: 1.737 ± 0.057
2.604AspVal: 2.604 ± 0.083
0.822AspTrp: 0.822 ± 0.042
1.685AspTyr: 1.685 ± 0.056
0.0AspXaa: 0.0 ± 0.0
Glu
3.738GluAla: 3.738 ± 0.091
0.558GluCys: 0.558 ± 0.031
3.351GluAsp: 3.351 ± 0.093
5.169GluGlu: 5.169 ± 0.113
2.834GluPhe: 2.834 ± 0.09
3.452GluGly: 3.452 ± 0.091
0.748GluHis: 0.748 ± 0.038
7.36GluIle: 7.36 ± 0.129
6.925GluLys: 6.925 ± 0.138
7.167GluLeu: 7.167 ± 0.115
1.264GluMet: 1.264 ± 0.056
4.815GluAsn: 4.815 ± 0.115
1.745GluPro: 1.745 ± 0.056
1.765GluGln: 1.765 ± 0.062
2.747GluArg: 2.747 ± 0.081
4.462GluSer: 4.462 ± 0.095
2.888GluThr: 2.888 ± 0.078
3.773GluVal: 3.773 ± 0.097
0.802GluTrp: 0.802 ± 0.04
1.739GluTyr: 1.739 ± 0.06
0.0GluXaa: 0.0 ± 0.0
Phe
2.533PheAla: 2.533 ± 0.074
0.74PheCys: 0.74 ± 0.044
2.92PheAsp: 2.92 ± 0.084
3.18PheGlu: 3.18 ± 0.081
2.789PhePhe: 2.789 ± 0.108
3.295PheGly: 3.295 ± 0.095
0.796PheHis: 0.796 ± 0.04
4.085PheIle: 4.085 ± 0.106
3.696PheLys: 3.696 ± 0.095
5.756PheLeu: 5.756 ± 0.151
0.852PheMet: 0.852 ± 0.04
3.122PheAsn: 3.122 ± 0.09
1.719PhePro: 1.719 ± 0.062
1.516PheGln: 1.516 ± 0.052
1.713PheArg: 1.713 ± 0.055
4.071PheSer: 4.071 ± 0.095
2.142PheThr: 2.142 ± 0.072
2.451PheVal: 2.451 ± 0.077
0.625PheTrp: 0.625 ± 0.034
1.546PheTyr: 1.546 ± 0.054
0.0PheXaa: 0.0 ± 0.0
Gly
3.71GlyAla: 3.71 ± 0.112
0.917GlyCys: 0.917 ± 0.046
3.094GlyAsp: 3.094 ± 0.08
3.615GlyGlu: 3.615 ± 0.088
3.587GlyPhe: 3.587 ± 0.082
4.645GlyGly: 4.645 ± 0.141
1.018GlyHis: 1.018 ± 0.046
5.984GlyIle: 5.984 ± 0.115
4.543GlyLys: 4.543 ± 0.088
6.612GlyLeu: 6.612 ± 0.129
1.449GlyMet: 1.449 ± 0.075
2.908GlyAsn: 2.908 ± 0.078
1.88GlyPro: 1.88 ± 0.07
1.771GlyGln: 1.771 ± 0.053
2.634GlyArg: 2.634 ± 0.079
4.571GlySer: 4.571 ± 0.108
2.979GlyThr: 2.979 ± 0.083
3.744GlyVal: 3.744 ± 0.091
0.961GlyTrp: 0.961 ± 0.045
1.987GlyTyr: 1.987 ± 0.064
0.0GlyXaa: 0.0 ± 0.0
His
0.794HisAla: 0.794 ± 0.041
0.24HisCys: 0.24 ± 0.023
0.651HisAsp: 0.651 ± 0.039
0.824HisGlu: 0.824 ± 0.043
0.744HisPhe: 0.744 ± 0.041
1.082HisGly: 1.082 ± 0.052
0.389HisHis: 0.389 ± 0.037
1.139HisIle: 1.139 ± 0.05
1.068HisLys: 1.068 ± 0.049
1.755HisLeu: 1.755 ± 0.067
0.258HisMet: 0.258 ± 0.029
0.808HisAsn: 0.808 ± 0.04
0.832HisPro: 0.832 ± 0.041
0.564HisGln: 0.564 ± 0.036
0.701HisArg: 0.701 ± 0.037
1.116HisSer: 1.116 ± 0.05
0.663HisThr: 0.663 ± 0.041
0.693HisVal: 0.693 ± 0.036
0.21HisTrp: 0.21 ± 0.021
0.443HisTyr: 0.443 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
5.198IleAla: 5.198 ± 0.124
1.171IleCys: 1.171 ± 0.057
5.278IleAsp: 5.278 ± 0.119
6.191IleGlu: 6.191 ± 0.11
4.867IlePhe: 4.867 ± 0.142
5.711IleGly: 5.711 ± 0.114
1.328IleHis: 1.328 ± 0.058
6.894IleIle: 6.894 ± 0.149
7.858IleLys: 7.858 ± 0.138
8.438IleLeu: 8.438 ± 0.152
1.278IleMet: 1.278 ± 0.056
6.586IleAsn: 6.586 ± 0.137
3.738IlePro: 3.738 ± 0.089
2.622IleGln: 2.622 ± 0.071
3.029IleArg: 3.029 ± 0.087
8.16IleSer: 8.16 ± 0.148
4.148IleThr: 4.148 ± 0.102
4.385IleVal: 4.385 ± 0.096
0.971IleTrp: 0.971 ± 0.047
2.549IleTyr: 2.549 ± 0.081
0.0IleXaa: 0.0 ± 0.0
Lys
3.841LysAla: 3.841 ± 0.092
0.792LysCys: 0.792 ± 0.041
4.615LysAsp: 4.615 ± 0.096
6.634LysGlu: 6.634 ± 0.131
3.801LysPhe: 3.801 ± 0.092
4.329LysGly: 4.329 ± 0.098
0.949LysHis: 0.949 ± 0.043
8.152LysIle: 8.152 ± 0.154
9.188LysLys: 9.188 ± 0.187
8.14LysLeu: 8.14 ± 0.146
1.586LysMet: 1.586 ± 0.062
7.791LysAsn: 7.791 ± 0.152
2.574LysPro: 2.574 ± 0.071
2.237LysGln: 2.237 ± 0.073
3.468LysArg: 3.468 ± 0.089
6.191LysSer: 6.191 ± 0.122
3.873LysThr: 3.873 ± 0.097
4.291LysVal: 4.291 ± 0.097
1.018LysTrp: 1.018 ± 0.043
2.584LysTyr: 2.584 ± 0.07
0.0LysXaa: 0.0 ± 0.0
Leu
6.233LeuAla: 6.233 ± 0.115
1.078LeuCys: 1.078 ± 0.041
5.645LeuAsp: 5.645 ± 0.101
7.431LeuGlu: 7.431 ± 0.142
4.837LeuPhe: 4.837 ± 0.122
6.594LeuGly: 6.594 ± 0.134
1.493LeuHis: 1.493 ± 0.063
10.589LeuIle: 10.589 ± 0.181
9.341LeuLys: 9.341 ± 0.158
10.947LeuLeu: 10.947 ± 0.187
2.042LeuMet: 2.042 ± 0.069
7.076LeuAsn: 7.076 ± 0.148
4.186LeuPro: 4.186 ± 0.111
3.033LeuGln: 3.033 ± 0.086
4.323LeuArg: 4.323 ± 0.113
8.539LeuSer: 8.539 ± 0.141
4.897LeuThr: 4.897 ± 0.093
5.786LeuVal: 5.786 ± 0.126
1.125LeuTrp: 1.125 ± 0.053
2.245LeuTyr: 2.245 ± 0.058
0.0LeuXaa: 0.0 ± 0.0
Met
1.344MetAla: 1.344 ± 0.056
0.137MetCys: 0.137 ± 0.016
0.873MetAsp: 0.873 ± 0.043
1.199MetGlu: 1.199 ± 0.059
0.675MetPhe: 0.675 ± 0.041
1.366MetGly: 1.366 ± 0.065
0.318MetHis: 0.318 ± 0.027
1.467MetIle: 1.467 ± 0.055
1.628MetLys: 1.628 ± 0.058
1.556MetLeu: 1.556 ± 0.062
0.357MetMet: 0.357 ± 0.027
1.237MetAsn: 1.237 ± 0.046
0.919MetPro: 0.919 ± 0.045
0.661MetGln: 0.661 ± 0.041
0.897MetArg: 0.897 ± 0.037
1.463MetSer: 1.463 ± 0.046
1.034MetThr: 1.034 ± 0.049
0.985MetVal: 0.985 ± 0.044
0.113MetTrp: 0.113 ± 0.017
0.343MetTyr: 0.343 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.773AsnAla: 2.773 ± 0.077
0.826AsnCys: 0.826 ± 0.038
3.045AsnAsp: 3.045 ± 0.081
3.966AsnGlu: 3.966 ± 0.098
3.595AsnPhe: 3.595 ± 0.095
3.273AsnGly: 3.273 ± 0.092
1.004AsnHis: 1.004 ± 0.043
5.933AsnIle: 5.933 ± 0.128
6.147AsnLys: 6.147 ± 0.122
7.729AsnLeu: 7.729 ± 0.147
1.159AsnMet: 1.159 ± 0.048
4.827AsnAsn: 4.827 ± 0.125
2.658AsnPro: 2.658 ± 0.079
2.372AsnGln: 2.372 ± 0.068
2.068AsnArg: 2.068 ± 0.067
5.214AsnSer: 5.214 ± 0.112
2.586AsnThr: 2.586 ± 0.068
2.477AsnVal: 2.477 ± 0.07
0.949AsnTrp: 0.949 ± 0.052
2.336AsnTyr: 2.336 ± 0.075
0.0AsnXaa: 0.0 ± 0.0
Pro
1.691ProAla: 1.691 ± 0.067
0.379ProCys: 0.379 ± 0.029
1.894ProAsp: 1.894 ± 0.061
2.582ProGlu: 2.582 ± 0.075
1.826ProPhe: 1.826 ± 0.064
2.374ProGly: 2.374 ± 0.081
0.635ProHis: 0.635 ± 0.037
3.249ProIle: 3.249 ± 0.083
2.672ProLys: 2.672 ± 0.074
4.031ProLeu: 4.031 ± 0.099
0.582ProMet: 0.582 ± 0.035
2.308ProAsn: 2.308 ± 0.071
1.209ProPro: 1.209 ± 0.055
1.116ProGln: 1.116 ± 0.057
1.264ProArg: 1.264 ± 0.051
2.942ProSer: 2.942 ± 0.077
1.814ProThr: 1.814 ± 0.058
2.09ProVal: 2.09 ± 0.063
0.516ProTrp: 0.516 ± 0.034
1.141ProTyr: 1.141 ± 0.045
0.0ProXaa: 0.0 ± 0.0
Gln
1.657GlnAla: 1.657 ± 0.066
0.26GlnCys: 0.26 ± 0.023
1.368GlnAsp: 1.368 ± 0.053
2.11GlnGlu: 2.11 ± 0.072
1.243GlnPhe: 1.243 ± 0.049
1.588GlnGly: 1.588 ± 0.058
0.351GlnHis: 0.351 ± 0.027
3.208GlnIle: 3.208 ± 0.078
3.122GlnLys: 3.122 ± 0.086
3.158GlnLeu: 3.158 ± 0.083
0.544GlnMet: 0.544 ± 0.034
2.05GlnAsn: 2.05 ± 0.064
0.945GlnPro: 0.945 ± 0.046
0.979GlnGln: 0.979 ± 0.055
1.366GlnArg: 1.366 ± 0.048
2.019GlnSer: 2.019 ± 0.061
1.441GlnThr: 1.441 ± 0.048
1.751GlnVal: 1.751 ± 0.074
0.369GlnTrp: 0.369 ± 0.026
0.822GlnTyr: 0.822 ± 0.046
0.0GlnXaa: 0.0 ± 0.0
Arg
2.005ArgAla: 2.005 ± 0.07
0.421ArgCys: 0.421 ± 0.03
2.009ArgAsp: 2.009 ± 0.064
2.694ArgGlu: 2.694 ± 0.08
1.919ArgPhe: 1.919 ± 0.059
2.32ArgGly: 2.32 ± 0.076
0.57ArgHis: 0.57 ± 0.04
3.46ArgIle: 3.46 ± 0.092
3.315ArgLys: 3.315 ± 0.083
4.18ArgLeu: 4.18 ± 0.099
0.848ArgMet: 0.848 ± 0.044
2.499ArgAsn: 2.499 ± 0.08
1.423ArgPro: 1.423 ± 0.064
1.239ArgGln: 1.239 ± 0.049
2.015ArgArg: 2.015 ± 0.063
2.678ArgSer: 2.678 ± 0.08
1.606ArgThr: 1.606 ± 0.057
2.255ArgVal: 2.255 ± 0.078
0.544ArgTrp: 0.544 ± 0.036
1.211ArgTyr: 1.211 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
3.591SerAla: 3.591 ± 0.087
0.907SerCys: 0.907 ± 0.046
3.755SerAsp: 3.755 ± 0.091
5.008SerGlu: 5.008 ± 0.109
4.123SerPhe: 4.123 ± 0.111
4.895SerGly: 4.895 ± 0.099
1.241SerHis: 1.241 ± 0.052
6.981SerIle: 6.981 ± 0.132
6.83SerLys: 6.83 ± 0.137
8.704SerLeu: 8.704 ± 0.155
1.54SerMet: 1.54 ± 0.052
5.022SerAsn: 5.022 ± 0.108
2.559SerPro: 2.559 ± 0.065
2.557SerGln: 2.557 ± 0.072
3.009SerArg: 3.009 ± 0.084
6.312SerSer: 6.312 ± 0.137
3.402SerThr: 3.402 ± 0.083
3.626SerVal: 3.626 ± 0.09
1.002SerTrp: 1.002 ± 0.048
2.257SerTyr: 2.257 ± 0.064
0.0SerXaa: 0.0 ± 0.0
Thr
2.602ThrAla: 2.602 ± 0.077
0.496ThrCys: 0.496 ± 0.036
2.09ThrAsp: 2.09 ± 0.072
2.435ThrGlu: 2.435 ± 0.068
2.209ThrPhe: 2.209 ± 0.073
3.257ThrGly: 3.257 ± 0.101
0.748ThrHis: 0.748 ± 0.04
3.882ThrIle: 3.882 ± 0.084
3.551ThrLys: 3.551 ± 0.093
4.744ThrLeu: 4.744 ± 0.096
0.685ThrMet: 0.685 ± 0.041
2.801ThrAsn: 2.801 ± 0.075
1.9ThrPro: 1.9 ± 0.058
1.276ThrGln: 1.276 ± 0.051
1.59ThrArg: 1.59 ± 0.065
3.567ThrSer: 3.567 ± 0.08
2.346ThrThr: 2.346 ± 0.073
2.346ThrVal: 2.346 ± 0.071
0.5ThrTrp: 0.5 ± 0.035
1.356ThrTyr: 1.356 ± 0.055
0.0ThrXaa: 0.0 ± 0.0
Val
3.241ValAla: 3.241 ± 0.078
0.603ValCys: 0.603 ± 0.032
3.071ValAsp: 3.071 ± 0.083
3.577ValGlu: 3.577 ± 0.082
2.654ValPhe: 2.654 ± 0.074
3.73ValGly: 3.73 ± 0.1
0.846ValHis: 0.846 ± 0.043
4.639ValIle: 4.639 ± 0.102
3.777ValLys: 3.777 ± 0.092
5.417ValLeu: 5.417 ± 0.11
1.018ValMet: 1.018 ± 0.054
3.069ValAsn: 3.069 ± 0.075
2.017ValPro: 2.017 ± 0.064
1.415ValGln: 1.415 ± 0.05
2.062ValArg: 2.062 ± 0.073
3.978ValSer: 3.978 ± 0.085
2.402ValThr: 2.402 ± 0.076
3.454ValVal: 3.454 ± 0.095
0.548ValTrp: 0.548 ± 0.032
1.183ValTyr: 1.183 ± 0.049
0.0ValXaa: 0.0 ± 0.0
Trp
0.649TrpAla: 0.649 ± 0.038
0.173TrpCys: 0.173 ± 0.018
0.669TrpAsp: 0.669 ± 0.042
0.915TrpGlu: 0.915 ± 0.048
0.617TrpPhe: 0.617 ± 0.035
0.734TrpGly: 0.734 ± 0.035
0.276TrpHis: 0.276 ± 0.021
1.231TrpIle: 1.231 ± 0.06
0.883TrpLys: 0.883 ± 0.048
1.431TrpLeu: 1.431 ± 0.054
0.268TrpMet: 0.268 ± 0.024
0.677TrpAsn: 0.677 ± 0.047
0.455TrpPro: 0.455 ± 0.031
0.445TrpGln: 0.445 ± 0.031
0.54TrpArg: 0.54 ± 0.034
0.848TrpSer: 0.848 ± 0.048
0.526TrpThr: 0.526 ± 0.035
0.703TrpVal: 0.703 ± 0.038
0.212TrpTrp: 0.212 ± 0.021
0.308TrpTyr: 0.308 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.362TyrAla: 1.362 ± 0.047
0.409TyrCys: 0.409 ± 0.028
1.415TyrAsp: 1.415 ± 0.053
2.056TyrGlu: 2.056 ± 0.056
1.524TyrPhe: 1.524 ± 0.065
2.136TyrGly: 2.136 ± 0.062
0.379TyrHis: 0.379 ± 0.031
1.951TyrIle: 1.951 ± 0.062
2.529TyrLys: 2.529 ± 0.086
3.567TyrLeu: 3.567 ± 0.081
0.502TyrMet: 0.502 ± 0.034
1.07TyrAsn: 1.07 ± 0.048
1.207TyrPro: 1.207 ± 0.051
1.02TyrGln: 1.02 ± 0.046
1.112TyrArg: 1.112 ± 0.053
2.43TyrSer: 2.43 ± 0.068
1.026TyrThr: 1.026 ± 0.049
1.395TyrVal: 1.395 ± 0.049
0.488TyrTrp: 0.488 ± 0.034
0.806TyrTyr: 0.806 ± 0.05
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1906 proteins (496198 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski