Amino acid dipepetide frequency for Rhodococcus phage REQ1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.496AlaAla: 11.496 ± 1.0
0.568AlaCys: 0.568 ± 0.226
7.137AlaAsp: 7.137 ± 0.644
6.695AlaGlu: 6.695 ± 0.856
3.284AlaPhe: 3.284 ± 0.402
8.78AlaGly: 8.78 ± 0.93
2.527AlaHis: 2.527 ± 0.478
5.495AlaIle: 5.495 ± 0.833
3.348AlaLys: 3.348 ± 0.408
9.159AlaLeu: 9.159 ± 0.916
1.895AlaMet: 1.895 ± 0.444
3.6AlaAsn: 3.6 ± 0.457
4.232AlaPro: 4.232 ± 0.662
3.6AlaGln: 3.6 ± 0.512
6.253AlaArg: 6.253 ± 0.621
6.316AlaSer: 6.316 ± 0.751
7.201AlaThr: 7.201 ± 0.896
7.137AlaVal: 7.137 ± 0.531
2.211AlaTrp: 2.211 ± 0.528
2.4AlaTyr: 2.4 ± 0.36
0.0AlaXaa: 0.0 ± 0.0
Cys
0.821CysAla: 0.821 ± 0.228
0.126CysCys: 0.126 ± 0.095
0.442CysAsp: 0.442 ± 0.169
0.442CysGlu: 0.442 ± 0.172
0.316CysPhe: 0.316 ± 0.116
1.39CysGly: 1.39 ± 0.395
0.126CysHis: 0.126 ± 0.095
0.316CysIle: 0.316 ± 0.139
0.316CysLys: 0.316 ± 0.144
0.442CysLeu: 0.442 ± 0.187
0.063CysMet: 0.063 ± 0.073
0.126CysAsn: 0.126 ± 0.088
0.695CysPro: 0.695 ± 0.206
0.316CysGln: 0.316 ± 0.124
1.39CysArg: 1.39 ± 0.479
0.505CysSer: 0.505 ± 0.175
0.632CysThr: 0.632 ± 0.174
0.632CysVal: 0.632 ± 0.241
0.126CysTrp: 0.126 ± 0.076
0.505CysTyr: 0.505 ± 0.179
0.0CysXaa: 0.0 ± 0.0
Asp
6.822AspAla: 6.822 ± 0.644
0.442AspCys: 0.442 ± 0.168
4.295AspAsp: 4.295 ± 0.652
4.927AspGlu: 4.927 ± 0.715
2.337AspPhe: 2.337 ± 0.365
6.064AspGly: 6.064 ± 0.578
1.516AspHis: 1.516 ± 0.266
3.032AspIle: 3.032 ± 0.413
1.769AspLys: 1.769 ± 0.285
6.001AspLeu: 6.001 ± 0.635
1.074AspMet: 1.074 ± 0.283
2.211AspAsn: 2.211 ± 0.354
3.537AspPro: 3.537 ± 0.538
1.958AspGln: 1.958 ± 0.366
3.979AspArg: 3.979 ± 0.642
2.463AspSer: 2.463 ± 0.34
4.169AspThr: 4.169 ± 0.894
5.053AspVal: 5.053 ± 0.6
1.2AspTrp: 1.2 ± 0.205
1.39AspTyr: 1.39 ± 0.365
0.0AspXaa: 0.0 ± 0.0
Glu
6.948GluAla: 6.948 ± 0.839
1.074GluCys: 1.074 ± 0.334
2.842GluAsp: 2.842 ± 0.465
4.421GluGlu: 4.421 ± 0.746
2.527GluPhe: 2.527 ± 0.418
3.411GluGly: 3.411 ± 0.395
1.516GluHis: 1.516 ± 0.32
3.727GluIle: 3.727 ± 0.471
1.832GluLys: 1.832 ± 0.442
4.611GluLeu: 4.611 ± 0.636
1.453GluMet: 1.453 ± 0.298
1.958GluAsn: 1.958 ± 0.331
2.842GluPro: 2.842 ± 0.503
3.221GluGln: 3.221 ± 0.537
4.295GluArg: 4.295 ± 0.707
2.211GluSer: 2.211 ± 0.372
3.158GluThr: 3.158 ± 0.519
4.99GluVal: 4.99 ± 0.6
1.39GluTrp: 1.39 ± 0.304
1.958GluTyr: 1.958 ± 0.406
0.0GluXaa: 0.0 ± 0.0
Phe
3.474PheAla: 3.474 ± 0.643
0.379PheCys: 0.379 ± 0.152
2.59PheAsp: 2.59 ± 0.389
2.148PheGlu: 2.148 ± 0.398
0.442PhePhe: 0.442 ± 0.145
2.211PheGly: 2.211 ± 0.425
0.632PheHis: 0.632 ± 0.2
0.568PheIle: 0.568 ± 0.195
1.137PheLys: 1.137 ± 0.248
2.716PheLeu: 2.716 ± 0.384
0.505PheMet: 0.505 ± 0.172
0.947PheAsn: 0.947 ± 0.286
1.2PhePro: 1.2 ± 0.247
1.137PheGln: 1.137 ± 0.257
2.211PheArg: 2.211 ± 0.468
1.326PheSer: 1.326 ± 0.338
2.527PheThr: 2.527 ± 0.526
2.653PheVal: 2.653 ± 0.366
0.632PheTrp: 0.632 ± 0.241
0.947PheTyr: 0.947 ± 0.23
0.0PheXaa: 0.0 ± 0.0
Gly
7.39GlyAla: 7.39 ± 0.983
0.884GlyCys: 0.884 ± 0.297
5.937GlyAsp: 5.937 ± 0.681
4.485GlyGlu: 4.485 ± 0.54
2.969GlyPhe: 2.969 ± 0.57
8.401GlyGly: 8.401 ± 1.82
2.021GlyHis: 2.021 ± 0.389
3.727GlyIle: 3.727 ± 0.526
3.284GlyLys: 3.284 ± 0.529
6.758GlyLeu: 6.758 ± 0.706
1.895GlyMet: 1.895 ± 0.361
3.727GlyAsn: 3.727 ± 0.632
2.906GlyPro: 2.906 ± 0.427
2.653GlyGln: 2.653 ± 0.361
5.053GlyArg: 5.053 ± 0.765
6.885GlySer: 6.885 ± 1.229
7.264GlyThr: 7.264 ± 1.283
6.695GlyVal: 6.695 ± 0.548
2.779GlyTrp: 2.779 ± 0.463
2.969GlyTyr: 2.969 ± 0.422
0.0GlyXaa: 0.0 ± 0.0
His
1.326HisAla: 1.326 ± 0.374
0.379HisCys: 0.379 ± 0.162
1.39HisAsp: 1.39 ± 0.299
1.326HisGlu: 1.326 ± 0.336
0.316HisPhe: 0.316 ± 0.135
1.832HisGly: 1.832 ± 0.359
1.2HisHis: 1.2 ± 0.338
0.884HisIle: 0.884 ± 0.304
0.947HisLys: 0.947 ± 0.271
1.895HisLeu: 1.895 ± 0.408
0.189HisMet: 0.189 ± 0.121
0.695HisAsn: 0.695 ± 0.238
1.39HisPro: 1.39 ± 0.369
0.568HisGln: 0.568 ± 0.207
1.579HisArg: 1.579 ± 0.381
0.947HisSer: 0.947 ± 0.27
1.769HisThr: 1.769 ± 0.295
1.579HisVal: 1.579 ± 0.307
0.316HisTrp: 0.316 ± 0.128
1.011HisTyr: 1.011 ± 0.271
0.0HisXaa: 0.0 ± 0.0
Ile
4.611IleAla: 4.611 ± 0.662
0.189IleCys: 0.189 ± 0.103
3.474IleAsp: 3.474 ± 0.535
3.537IleGlu: 3.537 ± 0.57
0.695IlePhe: 0.695 ± 0.195
3.663IleGly: 3.663 ± 0.521
0.884IleHis: 0.884 ± 0.193
1.39IleIle: 1.39 ± 0.332
1.263IleLys: 1.263 ± 0.354
3.411IleLeu: 3.411 ± 0.472
0.758IleMet: 0.758 ± 0.211
1.958IleAsn: 1.958 ± 0.464
2.527IlePro: 2.527 ± 0.487
1.705IleGln: 1.705 ± 0.281
3.411IleArg: 3.411 ± 0.513
2.4IleSer: 2.4 ± 0.367
3.79IleThr: 3.79 ± 0.494
3.284IleVal: 3.284 ± 0.546
0.695IleTrp: 0.695 ± 0.347
0.568IleTyr: 0.568 ± 0.19
0.0IleXaa: 0.0 ± 0.0
Lys
4.169LysAla: 4.169 ± 0.656
0.442LysCys: 0.442 ± 0.165
1.705LysAsp: 1.705 ± 0.279
2.148LysGlu: 2.148 ± 0.44
1.074LysPhe: 1.074 ± 0.259
3.158LysGly: 3.158 ± 0.49
0.695LysHis: 0.695 ± 0.231
1.579LysIle: 1.579 ± 0.362
1.39LysLys: 1.39 ± 0.316
2.779LysLeu: 2.779 ± 0.419
1.137LysMet: 1.137 ± 0.198
1.2LysAsn: 1.2 ± 0.265
2.021LysPro: 2.021 ± 0.38
1.074LysGln: 1.074 ± 0.26
2.148LysArg: 2.148 ± 0.53
1.832LysSer: 1.832 ± 0.402
2.337LysThr: 2.337 ± 0.37
3.221LysVal: 3.221 ± 0.431
0.505LysTrp: 0.505 ± 0.161
0.947LysTyr: 0.947 ± 0.267
0.0LysXaa: 0.0 ± 0.0
Leu
9.474LeuAla: 9.474 ± 0.821
1.011LeuCys: 1.011 ± 0.243
4.737LeuAsp: 4.737 ± 0.528
3.411LeuGlu: 3.411 ± 0.558
2.148LeuPhe: 2.148 ± 0.366
7.137LeuGly: 7.137 ± 0.606
1.895LeuHis: 1.895 ± 0.444
3.411LeuIle: 3.411 ± 0.527
3.221LeuLys: 3.221 ± 0.432
6.19LeuLeu: 6.19 ± 0.777
1.453LeuMet: 1.453 ± 0.381
2.211LeuAsn: 2.211 ± 0.408
3.6LeuPro: 3.6 ± 0.563
3.032LeuGln: 3.032 ± 0.495
7.137LeuArg: 7.137 ± 1.094
4.548LeuSer: 4.548 ± 0.424
5.685LeuThr: 5.685 ± 0.461
4.99LeuVal: 4.99 ± 0.709
2.274LeuTrp: 2.274 ± 0.489
2.021LeuTyr: 2.021 ± 0.444
0.0LeuXaa: 0.0 ± 0.0
Met
3.095MetAla: 3.095 ± 0.487
0.063MetCys: 0.063 ± 0.065
0.758MetAsp: 0.758 ± 0.221
0.821MetGlu: 0.821 ± 0.271
0.884MetPhe: 0.884 ± 0.266
1.263MetGly: 1.263 ± 0.263
0.442MetHis: 0.442 ± 0.166
0.821MetIle: 0.821 ± 0.26
0.505MetLys: 0.505 ± 0.208
1.326MetLeu: 1.326 ± 0.238
0.505MetMet: 0.505 ± 0.205
0.379MetAsn: 0.379 ± 0.13
0.947MetPro: 0.947 ± 0.195
0.632MetGln: 0.632 ± 0.201
1.895MetArg: 1.895 ± 0.3
1.958MetSer: 1.958 ± 0.326
2.274MetThr: 2.274 ± 0.434
0.884MetVal: 0.884 ± 0.237
0.189MetTrp: 0.189 ± 0.1
0.189MetTyr: 0.189 ± 0.091
0.0MetXaa: 0.0 ± 0.0
Asn
3.537AsnAla: 3.537 ± 0.792
0.189AsnCys: 0.189 ± 0.104
1.769AsnAsp: 1.769 ± 0.278
1.642AsnGlu: 1.642 ± 0.329
1.263AsnPhe: 1.263 ± 0.309
4.674AsnGly: 4.674 ± 0.753
0.821AsnHis: 0.821 ± 0.299
1.074AsnIle: 1.074 ± 0.249
1.2AsnLys: 1.2 ± 0.247
2.021AsnLeu: 2.021 ± 0.324
0.379AsnMet: 0.379 ± 0.154
1.074AsnAsn: 1.074 ± 0.397
2.337AsnPro: 2.337 ± 0.427
1.326AsnGln: 1.326 ± 0.276
1.958AsnArg: 1.958 ± 0.389
2.211AsnSer: 2.211 ± 0.447
2.969AsnThr: 2.969 ± 0.538
1.832AsnVal: 1.832 ± 0.412
0.505AsnTrp: 0.505 ± 0.232
0.695AsnTyr: 0.695 ± 0.187
0.0AsnXaa: 0.0 ± 0.0
Pro
5.053ProAla: 5.053 ± 0.601
0.253ProCys: 0.253 ± 0.124
3.284ProAsp: 3.284 ± 0.45
3.537ProGlu: 3.537 ± 0.49
1.263ProPhe: 1.263 ± 0.297
4.232ProGly: 4.232 ± 0.535
1.074ProHis: 1.074 ± 0.258
2.021ProIle: 2.021 ± 0.443
1.769ProLys: 1.769 ± 0.402
3.79ProLeu: 3.79 ± 0.581
0.821ProMet: 0.821 ± 0.201
1.39ProAsn: 1.39 ± 0.31
3.032ProPro: 3.032 ± 0.602
1.516ProGln: 1.516 ± 0.255
3.032ProArg: 3.032 ± 0.721
3.727ProSer: 3.727 ± 0.789
3.474ProThr: 3.474 ± 0.706
4.674ProVal: 4.674 ± 0.617
1.2ProTrp: 1.2 ± 0.298
1.263ProTyr: 1.263 ± 0.331
0.0ProXaa: 0.0 ± 0.0
Gln
3.537GlnAla: 3.537 ± 0.493
0.316GlnCys: 0.316 ± 0.158
1.958GlnAsp: 1.958 ± 0.43
1.579GlnGlu: 1.579 ± 0.306
1.895GlnPhe: 1.895 ± 0.29
3.032GlnGly: 3.032 ± 0.501
0.758GlnHis: 0.758 ± 0.216
1.832GlnIle: 1.832 ± 0.318
1.516GlnLys: 1.516 ± 0.289
2.906GlnLeu: 2.906 ± 0.568
0.821GlnMet: 0.821 ± 0.291
0.821GlnAsn: 0.821 ± 0.285
2.148GlnPro: 2.148 ± 0.345
1.326GlnGln: 1.326 ± 0.353
3.284GlnArg: 3.284 ± 0.526
2.084GlnSer: 2.084 ± 0.377
1.705GlnThr: 1.705 ± 0.289
2.337GlnVal: 2.337 ± 0.336
0.821GlnTrp: 0.821 ± 0.213
1.137GlnTyr: 1.137 ± 0.244
0.0GlnXaa: 0.0 ± 0.0
Arg
6.316ArgAla: 6.316 ± 0.629
0.568ArgCys: 0.568 ± 0.258
4.358ArgAsp: 4.358 ± 0.651
5.053ArgGlu: 5.053 ± 0.664
2.084ArgPhe: 2.084 ± 0.408
4.8ArgGly: 4.8 ± 0.673
1.2ArgHis: 1.2 ± 0.384
3.284ArgIle: 3.284 ± 0.447
2.59ArgLys: 2.59 ± 0.407
6.506ArgLeu: 6.506 ± 0.762
1.579ArgMet: 1.579 ± 0.316
1.769ArgAsn: 1.769 ± 0.308
2.842ArgPro: 2.842 ± 0.552
3.663ArgGln: 3.663 ± 0.604
6.506ArgArg: 6.506 ± 1.333
4.674ArgSer: 4.674 ± 0.691
4.864ArgThr: 4.864 ± 0.507
4.674ArgVal: 4.674 ± 0.624
1.074ArgTrp: 1.074 ± 0.229
1.642ArgTyr: 1.642 ± 0.365
0.0ArgXaa: 0.0 ± 0.0
Ser
6.19SerAla: 6.19 ± 0.95
0.695SerCys: 0.695 ± 0.212
3.537SerAsp: 3.537 ± 0.541
2.906SerGlu: 2.906 ± 0.443
1.769SerPhe: 1.769 ± 0.453
6.948SerGly: 6.948 ± 1.094
1.263SerHis: 1.263 ± 0.305
2.842SerIle: 2.842 ± 0.373
2.463SerLys: 2.463 ± 0.412
4.232SerLeu: 4.232 ± 0.533
1.832SerMet: 1.832 ± 0.318
2.842SerAsn: 2.842 ± 0.589
2.906SerPro: 2.906 ± 0.464
1.769SerGln: 1.769 ± 0.43
3.537SerArg: 3.537 ± 0.455
3.158SerSer: 3.158 ± 0.551
4.421SerThr: 4.421 ± 0.725
4.042SerVal: 4.042 ± 0.517
1.2SerTrp: 1.2 ± 0.26
1.326SerTyr: 1.326 ± 0.274
0.0SerXaa: 0.0 ± 0.0
Thr
8.464ThrAla: 8.464 ± 1.198
0.695ThrCys: 0.695 ± 0.204
4.548ThrAsp: 4.548 ± 0.838
3.853ThrGlu: 3.853 ± 0.554
1.453ThrPhe: 1.453 ± 0.315
7.643ThrGly: 7.643 ± 1.115
0.947ThrHis: 0.947 ± 0.272
3.095ThrIle: 3.095 ± 0.5
2.084ThrLys: 2.084 ± 0.339
5.495ThrLeu: 5.495 ± 0.577
1.705ThrMet: 1.705 ± 0.302
2.211ThrAsn: 2.211 ± 0.459
4.99ThrPro: 4.99 ± 0.763
2.4ThrGln: 2.4 ± 0.36
3.6ThrArg: 3.6 ± 0.472
4.99ThrSer: 4.99 ± 0.939
4.106ThrThr: 4.106 ± 0.721
6.822ThrVal: 6.822 ± 0.833
1.263ThrTrp: 1.263 ± 0.227
1.516ThrTyr: 1.516 ± 0.518
0.0ThrXaa: 0.0 ± 0.0
Val
7.137ValAla: 7.137 ± 0.71
0.758ValCys: 0.758 ± 0.278
6.443ValAsp: 6.443 ± 0.755
4.485ValGlu: 4.485 ± 0.587
2.463ValPhe: 2.463 ± 0.292
6.064ValGly: 6.064 ± 0.611
1.39ValHis: 1.39 ± 0.288
3.284ValIle: 3.284 ± 0.522
2.906ValLys: 2.906 ± 0.418
6.127ValLeu: 6.127 ± 0.8
1.137ValMet: 1.137 ± 0.249
2.148ValAsn: 2.148 ± 0.31
3.916ValPro: 3.916 ± 0.633
2.653ValGln: 2.653 ± 0.31
4.106ValArg: 4.106 ± 0.544
4.864ValSer: 4.864 ± 0.815
5.748ValThr: 5.748 ± 0.884
5.558ValVal: 5.558 ± 0.632
1.958ValTrp: 1.958 ± 0.597
1.705ValTyr: 1.705 ± 0.358
0.0ValXaa: 0.0 ± 0.0
Trp
1.705TrpAla: 1.705 ± 0.295
0.316TrpCys: 0.316 ± 0.126
1.39TrpAsp: 1.39 ± 0.623
1.39TrpGlu: 1.39 ± 0.444
0.821TrpPhe: 0.821 ± 0.206
1.453TrpGly: 1.453 ± 0.381
0.442TrpHis: 0.442 ± 0.154
0.695TrpIle: 0.695 ± 0.192
1.011TrpLys: 1.011 ± 0.242
1.516TrpLeu: 1.516 ± 0.275
0.379TrpMet: 0.379 ± 0.165
1.579TrpAsn: 1.579 ± 0.783
0.884TrpPro: 0.884 ± 0.256
0.758TrpGln: 0.758 ± 0.156
1.074TrpArg: 1.074 ± 0.226
1.326TrpSer: 1.326 ± 0.316
2.084TrpThr: 2.084 ± 0.456
1.579TrpVal: 1.579 ± 0.325
0.316TrpTrp: 0.316 ± 0.14
0.568TrpTyr: 0.568 ± 0.23
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.832TyrAla: 1.832 ± 0.284
0.379TyrCys: 0.379 ± 0.197
1.642TyrAsp: 1.642 ± 0.339
1.579TyrGlu: 1.579 ± 0.317
0.379TyrPhe: 0.379 ± 0.162
2.527TyrGly: 2.527 ± 0.393
0.189TyrHis: 0.189 ± 0.1
1.137TyrIle: 1.137 ± 0.261
1.011TyrLys: 1.011 ± 0.311
1.705TyrLeu: 1.705 ± 0.358
0.189TyrMet: 0.189 ± 0.097
0.632TyrAsn: 0.632 ± 0.146
1.453TyrPro: 1.453 ± 0.256
0.505TyrGln: 0.505 ± 0.181
3.6TyrArg: 3.6 ± 0.532
1.453TyrSer: 1.453 ± 0.319
1.705TyrThr: 1.705 ± 0.372
2.211TyrVal: 2.211 ± 0.382
0.632TyrTrp: 0.632 ± 0.222
0.126TyrTyr: 0.126 ± 0.099
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 85 proteins (15833 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski