Hydrogenobaculum phage 1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified dsDNA phages

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9P9G5|A0A0N9P9G5_9VIRU Uncharacterized protein OS=Hydrogenobaculum phage 1 OX=1732176 PE=4 SV=1
MM1 pKa = 7.66PKK3 pKa = 8.43TWKK6 pKa = 10.23EE7 pKa = 3.41LAEE10 pKa = 4.08EE11 pKa = 4.23LKK13 pKa = 11.06SLAEE17 pKa = 4.05VLLEE21 pKa = 3.91QAKK24 pKa = 9.53QAFRR28 pKa = 11.84EE29 pKa = 4.0GDD31 pKa = 3.28IEE33 pKa = 4.8RR34 pKa = 11.84GLMLTKK40 pKa = 10.3EE41 pKa = 4.13FRR43 pKa = 11.84SAIKK47 pKa = 9.13TAGDD51 pKa = 3.87LNMIAQGAGEE61 pKa = 4.14LEE63 pKa = 3.97EE64 pKa = 5.37DD65 pKa = 4.37FEE67 pKa = 5.02EE68 pKa = 4.5EE69 pKa = 4.9DD70 pKa = 3.36IEE72 pKa = 4.53KK73 pKa = 10.92ALGLEE78 pKa = 4.21DD79 pKa = 5.0EE80 pKa = 4.78EE81 pKa = 4.81DD82 pKa = 3.77LEE84 pKa = 4.41EE85 pKa = 4.6EE86 pKa = 4.25EE87 pKa = 4.92EE88 pKa = 3.95EE89 pKa = 5.15DD90 pKa = 4.09EE91 pKa = 4.22IEE93 pKa = 4.18EE94 pKa = 4.25YY95 pKa = 10.79EE96 pKa = 4.39EE97 pKa = 4.75SEE99 pKa = 4.06EE100 pKa = 5.86DD101 pKa = 3.45YY102 pKa = 11.61DD103 pKa = 5.38EE104 pKa = 4.47YY105 pKa = 11.35TDD107 pKa = 5.18DD108 pKa = 5.44DD109 pKa = 4.32EE110 pKa = 4.84EE111 pKa = 5.88LEE113 pKa = 4.25EE114 pKa = 5.14EE115 pKa = 4.58ADD117 pKa = 3.49NDD119 pKa = 3.93EE120 pKa = 5.07NEE122 pKa = 4.8LEE124 pKa = 4.04TDD126 pKa = 3.13YY127 pKa = 11.82DD128 pKa = 3.71FAEE131 pKa = 4.43EE132 pKa = 3.93EE133 pKa = 4.02RR134 pKa = 11.84KK135 pKa = 10.08KK136 pKa = 10.83IKK138 pKa = 9.1TQVGDD143 pKa = 3.98SIISLEE149 pKa = 4.04QILRR153 pKa = 11.84GIARR157 pKa = 11.84KK158 pKa = 8.38KK159 pKa = 7.7TT160 pKa = 3.37

Molecular weight: 18.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N9NIE1|A0A0N9NIE1_9VIRU Uncharacterized protein OS=Hydrogenobaculum phage 1 OX=1732176 PE=4 SV=1
MM1 pKa = 8.07RR2 pKa = 11.84GTQAPKK8 pKa = 10.88YY9 pKa = 8.08LTLWKK14 pKa = 10.31DD15 pKa = 3.61GRR17 pKa = 11.84WVFLKK22 pKa = 10.79RR23 pKa = 11.84EE24 pKa = 4.01DD25 pKa = 3.98FEE27 pKa = 4.44RR28 pKa = 11.84QGIVKK33 pKa = 10.13KK34 pKa = 10.11ISKK37 pKa = 9.02WLRR40 pKa = 11.84KK41 pKa = 9.31LIKK44 pKa = 10.2RR45 pKa = 3.98

Molecular weight: 5.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26

0

26

6043

42

1296

232.4

27.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.073 ± 0.469

0.629 ± 0.181

4.733 ± 0.289

9.432 ± 0.802

4.948 ± 0.396

5.246 ± 0.425

1.671 ± 0.218

7.066 ± 0.383

9.052 ± 0.379

10.26 ± 0.469

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.648 ± 0.373

3.674 ± 0.209

3.607 ± 0.427

4.435 ± 0.705

5.511 ± 0.399

4.667 ± 0.279

4.468 ± 0.159

5.726 ± 0.489

1.638 ± 0.222

4.385 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski