Mycobacterium phage Philonius

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nclasvirinae; Charlievirus; unclassified Charlievirus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4PD32|A0A2H4PD32_9CAUD Uncharacterized protein OS=Mycobacterium phage Philonius OX=2047843 GN=SEA_PHILONIUS_70 PE=4 SV=1
MM1 pKa = 7.73AKK3 pKa = 10.28LDD5 pKa = 3.88TLTDD9 pKa = 3.76DD10 pKa = 3.74FTVYY14 pKa = 10.9DD15 pKa = 4.1PGKK18 pKa = 7.82WTIDD22 pKa = 3.45DD23 pKa = 4.19SVGGDD28 pKa = 3.05IDD30 pKa = 4.0IGDD33 pKa = 3.53GRR35 pKa = 11.84AAINTNAGIEE45 pKa = 4.51GVFSVGAYY53 pKa = 10.22DD54 pKa = 4.68LLEE57 pKa = 4.63SYY59 pKa = 11.22AFAQFISFGFLYY71 pKa = 9.7PGKK74 pKa = 10.79GFGFSLNINSGGPNEE89 pKa = 4.37DD90 pKa = 2.86SAMFLCTEE98 pKa = 4.28SEE100 pKa = 4.55TEE102 pKa = 3.85GEE104 pKa = 4.27YY105 pKa = 11.3NLICLDD111 pKa = 3.54GAGFGAEE118 pKa = 4.19GTPIEE123 pKa = 4.4PVATGVTYY131 pKa = 10.74DD132 pKa = 3.82PAIHH136 pKa = 5.58SWLRR140 pKa = 11.84IRR142 pKa = 11.84EE143 pKa = 4.28SGGTIYY149 pKa = 10.47WEE151 pKa = 4.34TSPDD155 pKa = 3.6TAPLEE160 pKa = 4.22WSVMASGAHH169 pKa = 5.76TFTSAQATVQLGGVFAPAAFTVSSFNIAPTPPIPANAGNFFAFFF213 pKa = 4.14

Molecular weight: 22.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4PCZ2|A0A2H4PCZ2_9CAUD Uncharacterized protein OS=Mycobacterium phage Philonius OX=2047843 GN=SEA_PHILONIUS_43 PE=4 SV=1
MM1 pKa = 7.15SQRR4 pKa = 11.84EE5 pKa = 3.93PSGDD9 pKa = 3.58SVATAIRR16 pKa = 11.84IGLARR21 pKa = 11.84TNKK24 pKa = 8.43TQTALARR31 pKa = 11.84HH32 pKa = 6.35LNLSQPSIHH41 pKa = 6.56RR42 pKa = 11.84RR43 pKa = 11.84MAGKK47 pKa = 10.0VPWRR51 pKa = 11.84IHH53 pKa = 5.7EE54 pKa = 4.1LTAAAEE60 pKa = 4.2FLGITVPDD68 pKa = 4.28LLNEE72 pKa = 4.14KK73 pKa = 10.53ASAA76 pKa = 3.47

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

14155

41

1047

196.6

21.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.328 ± 0.634

1.201 ± 0.156

6.627 ± 0.22

5.567 ± 0.322

2.819 ± 0.181

8.81 ± 0.459

2.042 ± 0.16

4.535 ± 0.199

2.939 ± 0.222

7.828 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.013 ± 0.121

3.101 ± 0.218

6.295 ± 0.213

3.949 ± 0.177

6.796 ± 0.463

5.2 ± 0.217

6.93 ± 0.272

6.867 ± 0.272

1.922 ± 0.141

2.232 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski