Deinococcus geothermalis (strain DSM 11300 / AG-3a)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae; Deinococcus; Deinococcus geothermalis

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3006 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1J3V1|Q1J3V1_DEIGD Acyl-CoA dehydrogenase-like protein OS=Deinococcus geothermalis (strain DSM 11300 / AG-3a) OX=319795 GN=Dgeo_2399 PE=3 SV=1
MM1 pKa = 7.61TYY3 pKa = 10.72APSLSDD9 pKa = 2.86IVLPIGGSTPFYY21 pKa = 11.01VRR23 pKa = 11.84VTVPSGYY30 pKa = 10.53DD31 pKa = 3.16PSSGNQEE38 pKa = 3.82TAVIQITATTNTTPSDD54 pKa = 3.51TGSTSVTDD62 pKa = 3.28VTTVLRR68 pKa = 11.84SSASITKK75 pKa = 10.14SVALCSNPTCSSASPLSQGAPVAPNDD101 pKa = 3.75LLLYY105 pKa = 9.87TITASNTGEE114 pKa = 3.93APQNSVYY121 pKa = 10.55IADD124 pKa = 4.05TLPAEE129 pKa = 4.36TLFLSANAQGNGLPLLYY146 pKa = 10.12STDD149 pKa = 4.85NGLTWNTQAPSSLPNGTLLVGVDD172 pKa = 4.26SNNDD176 pKa = 3.27GQVNTNDD183 pKa = 3.66ALAAGQSFTVQIIVRR198 pKa = 11.84VRR200 pKa = 2.95

Molecular weight: 20.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1IYP3|Q1IYP3_DEIGD RNA polymerase sigma 28 subunit OS=Deinococcus geothermalis (strain DSM 11300 / AG-3a) OX=319795 GN=Dgeo_1346 PE=4 SV=1
MM1 pKa = 7.24RR2 pKa = 11.84RR3 pKa = 11.84VWLFPLAATLLVACGSLASPPAPTAGDD30 pKa = 3.77LLGAPTALQLAGRR43 pKa = 11.84SLKK46 pKa = 10.67AQASPTLAGNSFNVRR61 pKa = 11.84VRR63 pKa = 11.84VQASAAPLPPLTLTGVYY80 pKa = 9.92VVTEE84 pKa = 4.36DD85 pKa = 4.64GVWTADD91 pKa = 3.3TTQSEE96 pKa = 4.68RR97 pKa = 11.84SDD99 pKa = 3.93CAPKK103 pKa = 10.69VCLQGTGRR111 pKa = 11.84GAARR115 pKa = 11.84GLRR118 pKa = 11.84PGEE121 pKa = 4.05GVQVVVSLKK130 pKa = 10.26DD131 pKa = 3.32SKK133 pKa = 11.58GRR135 pKa = 11.84MLWLRR140 pKa = 11.84VPRR143 pKa = 11.84ANIGRR148 pKa = 11.84EE149 pKa = 3.9

Molecular weight: 15.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3006

0

3006

955421

30

3243

317.8

34.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.57 ± 0.062

0.591 ± 0.014

4.817 ± 0.032

5.762 ± 0.046

3.124 ± 0.029

8.965 ± 0.043

2.154 ± 0.024

3.404 ± 0.036

2.42 ± 0.035

12.038 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.708 ± 0.02

2.432 ± 0.033

6.03 ± 0.035

3.824 ± 0.034

7.829 ± 0.052

4.679 ± 0.031

5.964 ± 0.043

7.982 ± 0.041

1.366 ± 0.018

2.34 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski