Amino acid dipepetide frequency for Actinobacillus pleuropneumoniae serotype 5b (strain L20)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.87AlaAla: 6.87 ± 0.143
1.04AlaCys: 1.04 ± 0.044
4.615AlaAsp: 4.615 ± 0.091
6.666AlaGlu: 6.666 ± 0.121
3.565AlaPhe: 3.565 ± 0.091
5.962AlaGly: 5.962 ± 0.12
1.47AlaHis: 1.47 ± 0.049
6.328AlaIle: 6.328 ± 0.108
6.351AlaLys: 6.351 ± 0.115
9.597AlaLeu: 9.597 ± 0.154
2.45AlaMet: 2.45 ± 0.068
3.989AlaAsn: 3.989 ± 0.095
2.553AlaPro: 2.553 ± 0.072
4.205AlaGln: 4.205 ± 0.098
3.48AlaArg: 3.48 ± 0.089
4.262AlaSer: 4.262 ± 0.081
4.578AlaThr: 4.578 ± 0.128
6.395AlaVal: 6.395 ± 0.112
0.86AlaTrp: 0.86 ± 0.038
2.493AlaTyr: 2.493 ± 0.071
0.0AlaXaa: 0.0 ± 0.0
Cys
0.711CysAla: 0.711 ± 0.038
0.177CysCys: 0.177 ± 0.018
0.588CysAsp: 0.588 ± 0.031
0.651CysGlu: 0.651 ± 0.033
0.428CysPhe: 0.428 ± 0.026
0.935CysGly: 0.935 ± 0.047
0.295CysHis: 0.295 ± 0.024
0.564CysIle: 0.564 ± 0.029
0.487CysLys: 0.487 ± 0.029
1.0CysLeu: 1.0 ± 0.042
0.154CysMet: 0.154 ± 0.016
0.332CysAsn: 0.332 ± 0.024
0.428CysPro: 0.428 ± 0.028
0.404CysGln: 0.404 ± 0.029
0.467CysArg: 0.467 ± 0.03
0.614CysSer: 0.614 ± 0.037
0.442CysThr: 0.442 ± 0.025
0.671CysVal: 0.671 ± 0.041
0.109CysTrp: 0.109 ± 0.013
0.312CysTyr: 0.312 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
3.577AspAla: 3.577 ± 0.079
0.468AspCys: 0.468 ± 0.029
2.314AspAsp: 2.314 ± 0.073
3.628AspGlu: 3.628 ± 0.092
2.608AspPhe: 2.608 ± 0.063
3.43AspGly: 3.43 ± 0.159
0.921AspHis: 0.921 ± 0.04
3.545AspIle: 3.545 ± 0.081
3.442AspLys: 3.442 ± 0.083
5.259AspLeu: 5.259 ± 0.098
1.035AspMet: 1.035 ± 0.04
2.333AspAsn: 2.333 ± 0.079
2.035AspPro: 2.035 ± 0.061
1.778AspGln: 1.778 ± 0.051
2.228AspArg: 2.228 ± 0.062
2.557AspSer: 2.557 ± 0.067
2.169AspThr: 2.169 ± 0.07
3.39AspVal: 3.39 ± 0.086
0.754AspTrp: 0.754 ± 0.043
2.129AspTyr: 2.129 ± 0.059
0.0AspXaa: 0.0 ± 0.0
Glu
5.14GluAla: 5.14 ± 0.104
0.482GluCys: 0.482 ± 0.026
2.606GluAsp: 2.606 ± 0.079
3.893GluGlu: 3.893 ± 0.098
2.583GluPhe: 2.583 ± 0.068
3.517GluGly: 3.517 ± 0.074
1.327GluHis: 1.327 ± 0.046
4.674GluIle: 4.674 ± 0.091
5.119GluLys: 5.119 ± 0.105
6.705GluLeu: 6.705 ± 0.103
1.769GluMet: 1.769 ± 0.056
3.522GluAsn: 3.522 ± 0.083
1.951GluPro: 1.951 ± 0.065
4.435GluGln: 4.435 ± 0.096
3.354GluArg: 3.354 ± 0.076
3.176GluSer: 3.176 ± 0.085
3.236GluThr: 3.236 ± 0.082
4.17GluVal: 4.17 ± 0.093
0.785GluTrp: 0.785 ± 0.036
1.871GluTyr: 1.871 ± 0.052
0.0GluXaa: 0.0 ± 0.0
Phe
4.082PheAla: 4.082 ± 0.084
0.576PheCys: 0.576 ± 0.027
2.694PheAsp: 2.694 ± 0.064
2.9PheGlu: 2.9 ± 0.074
2.018PhePhe: 2.018 ± 0.067
3.606PheGly: 3.606 ± 0.077
0.832PheHis: 0.832 ± 0.035
3.118PheIle: 3.118 ± 0.086
2.273PheLys: 2.273 ± 0.063
3.964PheLeu: 3.964 ± 0.09
0.957PheMet: 0.957 ± 0.038
2.216PheAsn: 2.216 ± 0.06
1.505PhePro: 1.505 ± 0.049
1.402PheGln: 1.402 ± 0.055
1.58PheArg: 1.58 ± 0.046
3.308PheSer: 3.308 ± 0.091
2.348PheThr: 2.348 ± 0.063
3.089PheVal: 3.089 ± 0.075
0.582PheTrp: 0.582 ± 0.03
1.606PheTyr: 1.606 ± 0.049
0.0PheXaa: 0.0 ± 0.0
Gly
5.188GlyAla: 5.188 ± 0.112
0.788GlyCys: 0.788 ± 0.041
3.19GlyAsp: 3.19 ± 0.101
4.285GlyGlu: 4.285 ± 0.088
3.268GlyPhe: 3.268 ± 0.075
4.855GlyGly: 4.855 ± 0.123
1.242GlyHis: 1.242 ± 0.052
5.196GlyIle: 5.196 ± 0.099
5.217GlyLys: 5.217 ± 0.112
7.049GlyLeu: 7.049 ± 0.114
1.895GlyMet: 1.895 ± 0.062
2.881GlyAsn: 2.881 ± 0.107
1.12GlyPro: 1.12 ± 0.044
2.608GlyGln: 2.608 ± 0.066
2.844GlyArg: 2.844 ± 0.074
3.935GlySer: 3.935 ± 0.1
3.431GlyThr: 3.431 ± 0.102
5.323GlyVal: 5.323 ± 0.1
0.903GlyTrp: 0.903 ± 0.038
2.526GlyTyr: 2.526 ± 0.062
0.0GlyXaa: 0.0 ± 0.0
His
1.282HisAla: 1.282 ± 0.045
0.327HisCys: 0.327 ± 0.025
0.885HisAsp: 0.885 ± 0.041
0.878HisGlu: 0.878 ± 0.041
1.158HisPhe: 1.158 ± 0.051
1.281HisGly: 1.281 ± 0.056
0.647HisHis: 0.647 ± 0.037
1.519HisIle: 1.519 ± 0.052
1.146HisLys: 1.146 ± 0.043
2.238HisLeu: 2.238 ± 0.06
0.352HisMet: 0.352 ± 0.023
0.952HisAsn: 0.952 ± 0.043
1.04HisPro: 1.04 ± 0.039
1.173HisGln: 1.173 ± 0.047
0.948HisArg: 0.948 ± 0.037
1.316HisSer: 1.316 ± 0.047
1.095HisThr: 1.095 ± 0.042
0.859HisVal: 0.859 ± 0.038
0.299HisTrp: 0.299 ± 0.022
0.883HisTyr: 0.883 ± 0.04
0.0HisXaa: 0.0 ± 0.0
Ile
7.034IleAla: 7.034 ± 0.117
0.763IleCys: 0.763 ± 0.035
3.804IleAsp: 3.804 ± 0.08
4.733IleGlu: 4.733 ± 0.096
2.758IlePhe: 2.758 ± 0.073
5.269IleGly: 5.269 ± 0.104
1.312IleHis: 1.312 ± 0.044
4.205IleIle: 4.205 ± 0.087
3.618IleLys: 3.618 ± 0.077
6.1IleLeu: 6.1 ± 0.111
1.258IleMet: 1.258 ± 0.051
3.033IleAsn: 3.033 ± 0.087
2.674IlePro: 2.674 ± 0.065
2.66IleGln: 2.66 ± 0.064
3.015IleArg: 3.015 ± 0.072
4.529IleSer: 4.529 ± 0.089
3.752IleThr: 3.752 ± 0.094
4.445IleVal: 4.445 ± 0.089
0.637IleTrp: 0.637 ± 0.029
1.997IleTyr: 1.997 ± 0.06
0.0IleXaa: 0.0 ± 0.0
Lys
5.996LysAla: 5.996 ± 0.133
0.378LysCys: 0.378 ± 0.027
3.173LysAsp: 3.173 ± 0.105
3.641LysGlu: 3.641 ± 0.097
2.324LysPhe: 2.324 ± 0.071
4.088LysGly: 4.088 ± 0.107
1.272LysHis: 1.272 ± 0.05
3.93LysIle: 3.93 ± 0.086
3.689LysLys: 3.689 ± 0.091
6.234LysLeu: 6.234 ± 0.096
1.851LysMet: 1.851 ± 0.062
3.153LysAsn: 3.153 ± 0.068
2.531LysPro: 2.531 ± 0.068
3.476LysGln: 3.476 ± 0.086
3.006LysArg: 3.006 ± 0.084
3.324LysSer: 3.324 ± 0.082
3.413LysThr: 3.413 ± 0.082
4.3LysVal: 4.3 ± 0.086
0.642LysTrp: 0.642 ± 0.029
1.815LysTyr: 1.815 ± 0.061
0.0LysXaa: 0.0 ± 0.0
Leu
10.591LeuAla: 10.591 ± 0.151
0.928LeuCys: 0.928 ± 0.041
5.489LeuAsp: 5.489 ± 0.097
6.028LeuGlu: 6.028 ± 0.11
4.799LeuPhe: 4.799 ± 0.123
6.994LeuGly: 6.994 ± 0.108
1.944LeuHis: 1.944 ± 0.056
6.63LeuIle: 6.63 ± 0.126
6.059LeuLys: 6.059 ± 0.112
10.531LeuLeu: 10.531 ± 0.228
2.47LeuMet: 2.47 ± 0.07
5.177LeuAsn: 5.177 ± 0.099
4.713LeuPro: 4.713 ± 0.097
4.283LeuGln: 4.283 ± 0.083
4.543LeuArg: 4.543 ± 0.1
7.117LeuSer: 7.117 ± 0.13
6.07LeuThr: 6.07 ± 0.116
6.652LeuVal: 6.652 ± 0.118
1.026LeuTrp: 1.026 ± 0.042
2.616LeuTyr: 2.616 ± 0.065
0.0LeuXaa: 0.0 ± 0.0
Met
2.476MetAla: 2.476 ± 0.069
0.206MetCys: 0.206 ± 0.015
0.978MetAsp: 0.978 ± 0.038
1.183MetGlu: 1.183 ± 0.047
0.892MetPhe: 0.892 ± 0.037
1.54MetGly: 1.54 ± 0.053
0.432MetHis: 0.432 ± 0.028
1.476MetIle: 1.476 ± 0.049
1.626MetLys: 1.626 ± 0.052
2.698MetLeu: 2.698 ± 0.071
0.673MetMet: 0.673 ± 0.036
1.158MetAsn: 1.158 ± 0.046
1.101MetPro: 1.101 ± 0.045
1.256MetGln: 1.256 ± 0.044
1.109MetArg: 1.109 ± 0.043
1.513MetSer: 1.513 ± 0.048
1.41MetThr: 1.41 ± 0.051
1.474MetVal: 1.474 ± 0.052
0.224MetTrp: 0.224 ± 0.019
0.499MetTyr: 0.499 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
4.059AsnAla: 4.059 ± 0.114
0.401AsnCys: 0.401 ± 0.026
2.279AsnAsp: 2.279 ± 0.065
2.892AsnGlu: 2.892 ± 0.069
1.926AsnPhe: 1.926 ± 0.051
3.42AsnGly: 3.42 ± 0.118
0.958AsnHis: 0.958 ± 0.043
3.214AsnIle: 3.214 ± 0.074
2.646AsnLys: 2.646 ± 0.078
4.889AsnLeu: 4.889 ± 0.097
1.02AsnMet: 1.02 ± 0.033
2.172AsnAsn: 2.172 ± 0.104
2.399AsnPro: 2.399 ± 0.068
2.347AsnGln: 2.347 ± 0.066
2.156AsnArg: 2.156 ± 0.058
2.473AsnSer: 2.473 ± 0.075
2.264AsnThr: 2.264 ± 0.076
3.165AsnVal: 3.165 ± 0.096
0.637AsnTrp: 0.637 ± 0.033
1.554AsnTyr: 1.554 ± 0.052
0.0AsnXaa: 0.0 ± 0.0
Pro
2.906ProAla: 2.906 ± 0.08
0.273ProCys: 0.273 ± 0.024
1.763ProAsp: 1.763 ± 0.054
3.099ProGlu: 3.099 ± 0.071
1.857ProPhe: 1.857 ± 0.066
1.359ProGly: 1.359 ± 0.059
0.865ProHis: 0.865 ± 0.037
2.597ProIle: 2.597 ± 0.072
2.382ProLys: 2.382 ± 0.07
3.982ProLeu: 3.982 ± 0.094
0.957ProMet: 0.957 ± 0.037
2.236ProAsn: 2.236 ± 0.062
1.046ProPro: 1.046 ± 0.042
1.881ProGln: 1.881 ± 0.058
1.309ProArg: 1.309 ± 0.044
2.087ProSer: 2.087 ± 0.057
2.235ProThr: 2.235 ± 0.059
2.678ProVal: 2.678 ± 0.072
0.373ProTrp: 0.373 ± 0.024
1.344ProTyr: 1.344 ± 0.049
0.0ProXaa: 0.0 ± 0.0
Gln
4.953GlnAla: 4.953 ± 0.111
0.372GlnCys: 0.372 ± 0.022
2.101GlnAsp: 2.101 ± 0.055
2.493GlnGlu: 2.493 ± 0.061
2.159GlnPhe: 2.159 ± 0.063
2.913GlnGly: 2.913 ± 0.08
1.098GlnHis: 1.098 ± 0.04
3.231GlnIle: 3.231 ± 0.085
2.958GlnLys: 2.958 ± 0.075
4.971GlnLeu: 4.971 ± 0.08
1.089GlnMet: 1.089 ± 0.041
2.275GlnAsn: 2.275 ± 0.063
1.802GlnPro: 1.802 ± 0.065
3.251GlnGln: 3.251 ± 0.099
2.245GlnArg: 2.245 ± 0.06
2.411GlnSer: 2.411 ± 0.072
2.568GlnThr: 2.568 ± 0.07
3.03GlnVal: 3.03 ± 0.078
0.648GlnTrp: 0.648 ± 0.037
1.517GlnTyr: 1.517 ± 0.048
0.0GlnXaa: 0.0 ± 0.0
Arg
3.152ArgAla: 3.152 ± 0.081
0.404ArgCys: 0.404 ± 0.03
2.123ArgAsp: 2.123 ± 0.068
3.159ArgGlu: 3.159 ± 0.084
2.345ArgPhe: 2.345 ± 0.066
2.519ArgGly: 2.519 ± 0.061
1.081ArgHis: 1.081 ± 0.041
3.135ArgIle: 3.135 ± 0.069
2.654ArgLys: 2.654 ± 0.072
5.079ArgLeu: 5.079 ± 0.108
1.044ArgMet: 1.044 ± 0.041
2.0ArgAsn: 2.0 ± 0.051
1.606ArgPro: 1.606 ± 0.05
2.273ArgGln: 2.273 ± 0.068
2.201ArgArg: 2.201 ± 0.072
2.325ArgSer: 2.325 ± 0.064
2.132ArgThr: 2.132 ± 0.063
2.869ArgVal: 2.869 ± 0.071
0.522ArgTrp: 0.522 ± 0.033
1.798ArgTyr: 1.798 ± 0.053
0.0ArgXaa: 0.0 ± 0.0
Ser
4.97SerAla: 4.97 ± 0.084
0.505SerCys: 0.505 ± 0.032
2.881SerAsp: 2.881 ± 0.059
3.692SerGlu: 3.692 ± 0.076
2.668SerPhe: 2.668 ± 0.082
4.657SerGly: 4.657 ± 0.106
1.324SerHis: 1.324 ± 0.05
3.611SerIle: 3.611 ± 0.079
3.116SerLys: 3.116 ± 0.086
6.249SerLeu: 6.249 ± 0.101
1.339SerMet: 1.339 ± 0.045
2.499SerAsn: 2.499 ± 0.064
2.175SerPro: 2.175 ± 0.059
2.666SerGln: 2.666 ± 0.061
2.503SerArg: 2.503 ± 0.061
3.379SerSer: 3.379 ± 0.103
2.757SerThr: 2.757 ± 0.062
4.118SerVal: 4.118 ± 0.091
0.653SerTrp: 0.653 ± 0.026
1.88SerTyr: 1.88 ± 0.061
0.0SerXaa: 0.0 ± 0.0
Thr
5.019ThrAla: 5.019 ± 0.097
0.412ThrCys: 0.412 ± 0.025
2.766ThrAsp: 2.766 ± 0.088
3.566ThrGlu: 3.566 ± 0.079
2.344ThrPhe: 2.344 ± 0.065
3.841ThrGly: 3.841 ± 0.083
1.095ThrHis: 1.095 ± 0.042
3.322ThrIle: 3.322 ± 0.078
2.826ThrLys: 2.826 ± 0.076
5.994ThrLeu: 5.994 ± 0.101
1.041ThrMet: 1.041 ± 0.045
2.155ThrAsn: 2.155 ± 0.122
2.422ThrPro: 2.422 ± 0.055
2.376ThrGln: 2.376 ± 0.064
2.066ThrArg: 2.066 ± 0.061
2.655ThrSer: 2.655 ± 0.068
2.763ThrThr: 2.763 ± 0.105
3.803ThrVal: 3.803 ± 0.135
0.511ThrTrp: 0.511 ± 0.022
1.398ThrTyr: 1.398 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
6.286ValAla: 6.286 ± 0.121
0.733ValCys: 0.733 ± 0.029
3.265ValAsp: 3.265 ± 0.085
4.773ValGlu: 4.773 ± 0.101
2.712ValPhe: 2.712 ± 0.071
4.623ValGly: 4.623 ± 0.085
1.081ValHis: 1.081 ± 0.038
4.853ValIle: 4.853 ± 0.099
4.448ValLys: 4.448 ± 0.084
6.925ValLeu: 6.925 ± 0.129
1.806ValMet: 1.806 ± 0.056
3.016ValAsn: 3.016 ± 0.097
2.542ValPro: 2.542 ± 0.068
2.545ValGln: 2.545 ± 0.065
2.96ValArg: 2.96 ± 0.069
4.244ValSer: 4.244 ± 0.09
3.72ValThr: 3.72 ± 0.1
5.001ValVal: 5.001 ± 0.1
0.671ValTrp: 0.671 ± 0.031
1.808ValTyr: 1.808 ± 0.053
0.0ValXaa: 0.0 ± 0.0
Trp
0.882TrpAla: 0.882 ± 0.034
0.152TrpCys: 0.152 ± 0.017
0.527TrpAsp: 0.527 ± 0.028
0.527TrpGlu: 0.527 ± 0.029
0.591TrpPhe: 0.591 ± 0.033
0.746TrpGly: 0.746 ± 0.039
0.287TrpHis: 0.287 ± 0.022
0.74TrpIle: 0.74 ± 0.037
0.663TrpLys: 0.663 ± 0.027
1.674TrpLeu: 1.674 ± 0.06
0.2TrpMet: 0.2 ± 0.018
0.516TrpAsn: 0.516 ± 0.03
0.131TrpPro: 0.131 ± 0.014
1.046TrpGln: 1.046 ± 0.048
0.568TrpArg: 0.568 ± 0.034
0.527TrpSer: 0.527 ± 0.027
0.504TrpThr: 0.504 ± 0.029
0.72TrpVal: 0.72 ± 0.035
0.151TrpTrp: 0.151 ± 0.015
0.316TrpTyr: 0.316 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.554TyrAla: 2.554 ± 0.068
0.385TyrCys: 0.385 ± 0.025
1.557TyrAsp: 1.557 ± 0.048
1.628TyrGlu: 1.628 ± 0.053
1.653TyrPhe: 1.653 ± 0.054
2.189TyrGly: 2.189 ± 0.062
0.794TyrHis: 0.794 ± 0.04
1.711TyrIle: 1.711 ± 0.059
1.514TyrLys: 1.514 ± 0.047
3.52TyrLeu: 3.52 ± 0.099
0.565TyrMet: 0.565 ± 0.031
1.258TyrAsn: 1.258 ± 0.047
1.458TyrPro: 1.458 ± 0.05
2.02TyrGln: 2.02 ± 0.062
1.838TyrArg: 1.838 ± 0.06
1.889TyrSer: 1.889 ± 0.057
1.49TyrThr: 1.49 ± 0.05
1.86TyrVal: 1.86 ± 0.048
0.479TyrTrp: 0.479 ± 0.028
1.021TyrTyr: 1.021 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2004 proteins (651114 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski