Bombyx mori densovirus 5 (isolate Shinshu) (BmDNV-5)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Quintoviricetes; Piccovirales; Parvoviridae; Densovirinae; Iteradensovirus; Lepidopteran iteradensovirus 1; Bombyx mori densovirus; Bombyx mori densovirus 5

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9JFY3|Q9JFY3_BMDNS Nonstructural protein OS=Bombyx mori densovirus 5 (isolate Shinshu) OX=648251 GN=orf1 PE=4 SV=1
MM1 pKa = 7.08FRR3 pKa = 11.84VDD5 pKa = 5.24RR6 pKa = 11.84VDD8 pKa = 3.85RR9 pKa = 11.84DD10 pKa = 3.97CLILPWIDD18 pKa = 3.14VKK20 pKa = 11.27APPEE24 pKa = 4.15APAFIIVPSAGVKK37 pKa = 8.89YY38 pKa = 10.89VEE40 pKa = 4.59MALGWFFKK48 pKa = 11.02LFFFVSTPFDD58 pKa = 3.57VVIDD62 pKa = 3.58FLFRR66 pKa = 11.84VVFICLEE73 pKa = 3.79LDD75 pKa = 3.37GRR77 pKa = 11.84FLRR80 pKa = 11.84FSSSLSDD87 pKa = 5.29SDD89 pKa = 5.75WLDD92 pKa = 3.16CWLSAVSVSSRR103 pKa = 11.84YY104 pKa = 9.95DD105 pKa = 3.24SEE107 pKa = 5.18IVVGWVSSTNVFWVVFRR124 pKa = 11.84SFICFMEE131 pKa = 4.7PNSVRR136 pKa = 11.84TSAIKK141 pKa = 10.45SSPDD145 pKa = 3.33FVNHH149 pKa = 5.4FTKK152 pKa = 10.65SVAWSKK158 pKa = 11.47LNGVVV163 pKa = 3.63

Molecular weight: 18.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9JFY0|Q9JFY0_BMDNS Capsid protein OS=Bombyx mori densovirus 5 (isolate Shinshu) OX=648251 GN=orf2 PE=4 SV=1
MM1 pKa = 7.84LILLVNFCLLWSLLKK16 pKa = 10.46SLQMKK21 pKa = 8.41FQVKK25 pKa = 8.36QRR27 pKa = 11.84PEE29 pKa = 3.83YY30 pKa = 9.32LQKK33 pKa = 11.04LEE35 pKa = 4.17TVMMSTIQDD44 pKa = 3.6WDD46 pKa = 3.34TMMKK50 pKa = 9.93VVSAEE55 pKa = 3.79EE56 pKa = 3.76EE57 pKa = 4.04MIEE60 pKa = 4.14FLTEE64 pKa = 3.62EE65 pKa = 4.61FSAFIQKK72 pKa = 9.81DD73 pKa = 3.73CKK75 pKa = 10.93NNSLEE80 pKa = 4.08EE81 pKa = 4.24LYY83 pKa = 9.59EE84 pKa = 4.27TVDD87 pKa = 3.57EE88 pKa = 4.72PKK90 pKa = 10.5LVHH93 pKa = 7.04SSLATMKK100 pKa = 10.82YY101 pKa = 9.46YY102 pKa = 9.86LTLILQNKK110 pKa = 8.8AKK112 pKa = 8.6EE113 pKa = 4.28TTDD116 pKa = 3.59LKK118 pKa = 10.97GHH120 pKa = 5.75SLSVINKK127 pKa = 6.79WNSVIKK133 pKa = 9.91TPLKK137 pKa = 10.34LLSNEE142 pKa = 4.48KK143 pKa = 9.85EE144 pKa = 3.72ASTNSFEE151 pKa = 4.08RR152 pKa = 11.84WNAEE156 pKa = 3.78WEE158 pKa = 4.34SSQISYY164 pKa = 11.4AEE166 pKa = 4.19LLRR169 pKa = 11.84IIQLMQEE176 pKa = 4.18EE177 pKa = 6.19FYY179 pKa = 8.18TTPLSLDD186 pKa = 3.17QATDD190 pKa = 3.63FVKK193 pKa = 10.18WFTKK197 pKa = 10.39SGEE200 pKa = 3.94LLIAEE205 pKa = 4.31VRR207 pKa = 11.84TLFGSMKK214 pKa = 9.93QIKK217 pKa = 8.26EE218 pKa = 3.87RR219 pKa = 11.84KK220 pKa = 4.88TTQKK224 pKa = 9.55TLVEE228 pKa = 4.37DD229 pKa = 3.7THH231 pKa = 6.54PTTISEE237 pKa = 4.47SYY239 pKa = 10.89RR240 pKa = 11.84LDD242 pKa = 3.23TDD244 pKa = 3.67TAEE247 pKa = 4.59SQQSSQSEE255 pKa = 4.37SEE257 pKa = 4.01RR258 pKa = 11.84EE259 pKa = 3.73EE260 pKa = 3.79EE261 pKa = 4.0NRR263 pKa = 11.84RR264 pKa = 11.84NRR266 pKa = 11.84PSSSRR271 pKa = 11.84HH272 pKa = 4.68INTTRR277 pKa = 11.84KK278 pKa = 9.46RR279 pKa = 11.84KK280 pKa = 10.31SITTSKK286 pKa = 11.08GVLTKK291 pKa = 10.56KK292 pKa = 10.4KK293 pKa = 10.21SLKK296 pKa = 8.88NQPRR300 pKa = 11.84AISTYY305 pKa = 7.54FTPADD310 pKa = 3.77GTIMNAGASGGALTSIQGRR329 pKa = 11.84IRR331 pKa = 11.84QSLSTRR337 pKa = 11.84STRR340 pKa = 11.84NIYY343 pKa = 8.82ATLCSITQNGPDD355 pKa = 4.12GLCTSKK361 pKa = 10.41WPTDD365 pKa = 3.64PNSNMFIEE373 pKa = 4.83LNLFAKK379 pKa = 9.9NQYY382 pKa = 9.77NPSDD386 pKa = 3.35TRR388 pKa = 11.84NNWKK392 pKa = 10.11RR393 pKa = 11.84ATIRR397 pKa = 11.84MKK399 pKa = 10.76VDD401 pKa = 3.24LPDD404 pKa = 3.83NSRR407 pKa = 11.84HH408 pKa = 5.81AYY410 pKa = 7.51QLKK413 pKa = 10.32EE414 pKa = 4.09IIDD417 pKa = 3.91AQMSRR422 pKa = 11.84MEE424 pKa = 4.46DD425 pKa = 3.26HH426 pKa = 7.62PEE428 pKa = 3.72TEE430 pKa = 4.56TQSKK434 pKa = 10.77SSWSNSNSGQHH445 pKa = 6.23GPWNTYY451 pKa = 8.41

Molecular weight: 51.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2040

163

754

510.0

58.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.539 ± 0.862

1.127 ± 0.32

5.539 ± 0.528

7.402 ± 1.202

4.853 ± 1.312

5.098 ± 1.083

2.157 ± 0.365

5.637 ± 0.149

5.98 ± 0.64

7.108 ± 0.693

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.157 ± 0.329

5.931 ± 0.691

5.147 ± 0.564

4.216 ± 0.823

6.275 ± 0.67

7.5 ± 1.588

6.863 ± 1.18

5.49 ± 1.761

1.863 ± 0.518

4.118 ± 0.837
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski