Amino acid dipepetide frequency for Arthrobacter phage Loretta

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.593AlaAla: 8.593 ± 1.355
0.335AlaCys: 0.335 ± 0.15
5.022AlaAsp: 5.022 ± 0.506
5.636AlaGlu: 5.636 ± 0.556
3.181AlaPhe: 3.181 ± 0.458
6.082AlaGly: 6.082 ± 1.209
1.897AlaHis: 1.897 ± 0.392
5.636AlaIle: 5.636 ± 0.656
5.803AlaLys: 5.803 ± 0.797
6.919AlaLeu: 6.919 ± 0.729
3.236AlaMet: 3.236 ± 0.641
4.464AlaAsn: 4.464 ± 0.482
2.902AlaPro: 2.902 ± 0.445
2.957AlaGln: 2.957 ± 0.389
4.52AlaArg: 4.52 ± 0.533
5.245AlaSer: 5.245 ± 0.749
6.026AlaThr: 6.026 ± 0.644
6.361AlaVal: 6.361 ± 0.64
1.339AlaTrp: 1.339 ± 0.311
2.902AlaTyr: 2.902 ± 0.338
0.0AlaXaa: 0.0 ± 0.0
Cys
0.391CysAla: 0.391 ± 0.173
0.112CysCys: 0.112 ± 0.083
0.502CysAsp: 0.502 ± 0.162
0.446CysGlu: 0.446 ± 0.162
0.279CysPhe: 0.279 ± 0.134
0.558CysGly: 0.558 ± 0.247
0.167CysHis: 0.167 ± 0.095
0.446CysIle: 0.446 ± 0.173
0.112CysLys: 0.112 ± 0.073
0.502CysLeu: 0.502 ± 0.205
0.112CysMet: 0.112 ± 0.088
0.167CysAsn: 0.167 ± 0.102
0.335CysPro: 0.335 ± 0.145
0.112CysGln: 0.112 ± 0.093
0.391CysArg: 0.391 ± 0.172
0.391CysSer: 0.391 ± 0.164
0.223CysThr: 0.223 ± 0.111
0.167CysVal: 0.167 ± 0.089
0.056CysTrp: 0.056 ± 0.05
0.056CysTyr: 0.056 ± 0.061
0.0CysXaa: 0.0 ± 0.0
Asp
6.026AspAla: 6.026 ± 0.568
0.223AspCys: 0.223 ± 0.116
3.125AspAsp: 3.125 ± 0.441
4.297AspGlu: 4.297 ± 0.493
2.065AspPhe: 2.065 ± 0.514
4.91AspGly: 4.91 ± 0.527
0.725AspHis: 0.725 ± 0.207
3.739AspIle: 3.739 ± 0.602
2.846AspLys: 2.846 ± 0.414
4.185AspLeu: 4.185 ± 0.529
1.841AspMet: 1.841 ± 0.283
3.125AspAsn: 3.125 ± 0.572
2.902AspPro: 2.902 ± 0.491
1.897AspGln: 1.897 ± 0.43
2.902AspArg: 2.902 ± 0.585
3.292AspSer: 3.292 ± 0.269
2.678AspThr: 2.678 ± 0.377
4.185AspVal: 4.185 ± 0.627
0.781AspTrp: 0.781 ± 0.215
1.562AspTyr: 1.562 ± 0.361
0.0AspXaa: 0.0 ± 0.0
Glu
4.687GluAla: 4.687 ± 0.536
0.391GluCys: 0.391 ± 0.175
4.129GluAsp: 4.129 ± 0.547
5.357GluGlu: 5.357 ± 0.786
2.957GluPhe: 2.957 ± 0.43
4.241GluGly: 4.241 ± 0.454
1.507GluHis: 1.507 ± 0.356
4.352GluIle: 4.352 ± 0.435
4.018GluLys: 4.018 ± 0.582
4.91GluLeu: 4.91 ± 0.675
2.623GluMet: 2.623 ± 0.39
2.734GluAsn: 2.734 ± 0.453
2.678GluPro: 2.678 ± 0.421
2.176GluGln: 2.176 ± 0.313
3.46GluArg: 3.46 ± 0.483
3.125GluSer: 3.125 ± 0.437
3.794GluThr: 3.794 ± 0.511
5.022GluVal: 5.022 ± 0.754
1.172GluTrp: 1.172 ± 0.228
1.507GluTyr: 1.507 ± 0.261
0.0GluXaa: 0.0 ± 0.0
Phe
2.678PheAla: 2.678 ± 0.408
0.167PheCys: 0.167 ± 0.098
2.567PheAsp: 2.567 ± 0.423
2.344PheGlu: 2.344 ± 0.428
1.283PhePhe: 1.283 ± 0.289
3.013PheGly: 3.013 ± 0.435
0.614PheHis: 0.614 ± 0.243
1.786PheIle: 1.786 ± 0.398
2.567PheLys: 2.567 ± 0.347
3.236PheLeu: 3.236 ± 0.47
1.06PheMet: 1.06 ± 0.311
2.12PheAsn: 2.12 ± 0.332
1.507PhePro: 1.507 ± 0.348
1.172PheGln: 1.172 ± 0.21
2.232PheArg: 2.232 ± 0.407
2.734PheSer: 2.734 ± 0.4
2.288PheThr: 2.288 ± 0.472
2.79PheVal: 2.79 ± 0.359
0.446PheTrp: 0.446 ± 0.164
1.395PheTyr: 1.395 ± 0.32
0.0PheXaa: 0.0 ± 0.0
Gly
6.473GlyAla: 6.473 ± 0.813
0.223GlyCys: 0.223 ± 0.121
4.687GlyAsp: 4.687 ± 0.649
4.799GlyGlu: 4.799 ± 0.425
3.739GlyPhe: 3.739 ± 0.537
6.696GlyGly: 6.696 ± 2.026
1.172GlyHis: 1.172 ± 0.297
4.408GlyIle: 4.408 ± 0.68
4.966GlyLys: 4.966 ± 0.528
6.305GlyLeu: 6.305 ± 1.278
2.567GlyMet: 2.567 ± 0.578
3.013GlyAsn: 3.013 ± 0.436
1.786GlyPro: 1.786 ± 0.336
1.73GlyGln: 1.73 ± 0.344
2.455GlyArg: 2.455 ± 0.391
4.91GlySer: 4.91 ± 0.751
6.082GlyThr: 6.082 ± 0.968
5.803GlyVal: 5.803 ± 0.669
1.562GlyTrp: 1.562 ± 0.299
2.734GlyTyr: 2.734 ± 0.425
0.0GlyXaa: 0.0 ± 0.0
His
1.953HisAla: 1.953 ± 0.519
0.167HisCys: 0.167 ± 0.104
0.893HisAsp: 0.893 ± 0.264
1.339HisGlu: 1.339 ± 0.311
0.614HisPhe: 0.614 ± 0.239
1.395HisGly: 1.395 ± 0.401
0.502HisHis: 0.502 ± 0.16
1.451HisIle: 1.451 ± 0.31
1.116HisLys: 1.116 ± 0.28
1.06HisLeu: 1.06 ± 0.201
0.502HisMet: 0.502 ± 0.168
0.725HisAsn: 0.725 ± 0.235
1.004HisPro: 1.004 ± 0.202
0.781HisGln: 0.781 ± 0.269
0.781HisArg: 0.781 ± 0.242
1.116HisSer: 1.116 ± 0.308
0.67HisThr: 0.67 ± 0.214
1.116HisVal: 1.116 ± 0.288
0.167HisTrp: 0.167 ± 0.099
1.395HisTyr: 1.395 ± 0.316
0.0HisXaa: 0.0 ± 0.0
Ile
5.468IleAla: 5.468 ± 0.928
0.279IleCys: 0.279 ± 0.159
4.073IleAsp: 4.073 ± 0.441
3.906IleGlu: 3.906 ± 0.511
1.562IlePhe: 1.562 ± 0.351
3.85IleGly: 3.85 ± 0.686
1.06IleHis: 1.06 ± 0.213
2.678IleIle: 2.678 ± 0.427
4.297IleLys: 4.297 ± 0.535
5.189IleLeu: 5.189 ± 0.607
1.786IleMet: 1.786 ± 0.334
3.125IleAsn: 3.125 ± 0.413
2.902IlePro: 2.902 ± 0.472
1.73IleGln: 1.73 ± 0.287
2.734IleArg: 2.734 ± 0.394
3.906IleSer: 3.906 ± 0.437
4.018IleThr: 4.018 ± 0.439
4.799IleVal: 4.799 ± 0.541
0.67IleTrp: 0.67 ± 0.198
1.507IleTyr: 1.507 ± 0.232
0.0IleXaa: 0.0 ± 0.0
Lys
5.971LysAla: 5.971 ± 0.626
0.335LysCys: 0.335 ± 0.114
2.846LysAsp: 2.846 ± 0.649
4.966LysGlu: 4.966 ± 0.639
2.678LysPhe: 2.678 ± 0.38
4.687LysGly: 4.687 ± 0.53
1.339LysHis: 1.339 ± 0.315
3.348LysIle: 3.348 ± 0.642
5.803LysLys: 5.803 ± 0.918
5.971LysLeu: 5.971 ± 0.715
2.567LysMet: 2.567 ± 0.373
2.065LysAsn: 2.065 ± 0.351
2.902LysPro: 2.902 ± 0.49
2.288LysGln: 2.288 ± 0.408
3.962LysArg: 3.962 ± 0.612
4.073LysSer: 4.073 ± 0.559
3.683LysThr: 3.683 ± 0.503
3.627LysVal: 3.627 ± 0.436
1.06LysTrp: 1.06 ± 0.216
1.786LysTyr: 1.786 ± 0.282
0.0LysXaa: 0.0 ± 0.0
Leu
7.087LeuAla: 7.087 ± 0.913
0.335LeuCys: 0.335 ± 0.145
5.078LeuAsp: 5.078 ± 0.569
4.799LeuGlu: 4.799 ± 0.561
3.013LeuPhe: 3.013 ± 0.51
6.082LeuGly: 6.082 ± 1.125
1.451LeuHis: 1.451 ± 0.33
5.915LeuIle: 5.915 ± 0.518
5.413LeuLys: 5.413 ± 0.74
5.189LeuLeu: 5.189 ± 0.793
1.841LeuMet: 1.841 ± 0.286
4.073LeuAsn: 4.073 ± 0.555
3.292LeuPro: 3.292 ± 0.413
2.232LeuGln: 2.232 ± 0.445
3.739LeuArg: 3.739 ± 0.501
5.189LeuSer: 5.189 ± 0.5
4.743LeuThr: 4.743 ± 0.529
5.189LeuVal: 5.189 ± 0.571
0.781LeuTrp: 0.781 ± 0.25
2.399LeuTyr: 2.399 ± 0.413
0.0LeuXaa: 0.0 ± 0.0
Met
3.515MetAla: 3.515 ± 0.48
0.223MetCys: 0.223 ± 0.15
1.172MetAsp: 1.172 ± 0.237
1.228MetGlu: 1.228 ± 0.233
1.06MetPhe: 1.06 ± 0.337
2.623MetGly: 2.623 ± 0.533
0.446MetHis: 0.446 ± 0.176
2.065MetIle: 2.065 ± 0.473
1.953MetLys: 1.953 ± 0.29
2.511MetLeu: 2.511 ± 0.39
1.116MetMet: 1.116 ± 0.444
1.172MetAsn: 1.172 ± 0.261
1.395MetPro: 1.395 ± 0.239
0.837MetGln: 0.837 ± 0.366
1.116MetArg: 1.116 ± 0.311
3.348MetSer: 3.348 ± 0.416
2.232MetThr: 2.232 ± 0.479
1.674MetVal: 1.674 ± 0.331
0.335MetTrp: 0.335 ± 0.132
0.893MetTyr: 0.893 ± 0.236
0.0MetXaa: 0.0 ± 0.0
Asn
3.571AsnAla: 3.571 ± 0.536
0.335AsnCys: 0.335 ± 0.169
2.957AsnAsp: 2.957 ± 0.46
3.236AsnGlu: 3.236 ± 0.542
1.618AsnPhe: 1.618 ± 0.323
4.743AsnGly: 4.743 ± 0.68
0.893AsnHis: 0.893 ± 0.275
2.344AsnIle: 2.344 ± 0.511
2.79AsnLys: 2.79 ± 0.407
3.46AsnLeu: 3.46 ± 0.503
1.395AsnMet: 1.395 ± 0.338
2.12AsnAsn: 2.12 ± 0.378
3.013AsnPro: 3.013 ± 0.391
1.897AsnGln: 1.897 ± 0.371
2.344AsnArg: 2.344 ± 0.468
3.292AsnSer: 3.292 ± 0.524
2.957AsnThr: 2.957 ± 0.361
2.734AsnVal: 2.734 ± 0.418
0.725AsnTrp: 0.725 ± 0.225
1.786AsnTyr: 1.786 ± 0.305
0.0AsnXaa: 0.0 ± 0.0
Pro
3.181ProAla: 3.181 ± 0.496
0.446ProCys: 0.446 ± 0.185
1.953ProAsp: 1.953 ± 0.365
3.962ProGlu: 3.962 ± 0.584
1.004ProPhe: 1.004 ± 0.228
3.46ProGly: 3.46 ± 0.576
0.725ProHis: 0.725 ± 0.204
2.12ProIle: 2.12 ± 0.283
2.79ProLys: 2.79 ± 0.488
2.176ProLeu: 2.176 ± 0.346
1.06ProMet: 1.06 ± 0.273
2.12ProAsn: 2.12 ± 0.471
1.562ProPro: 1.562 ± 0.379
0.893ProGln: 0.893 ± 0.227
2.009ProArg: 2.009 ± 0.36
3.181ProSer: 3.181 ± 0.419
2.957ProThr: 2.957 ± 0.423
4.073ProVal: 4.073 ± 0.587
0.391ProTrp: 0.391 ± 0.147
1.283ProTyr: 1.283 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
2.455GlnAla: 2.455 ± 0.404
0.167GlnCys: 0.167 ± 0.114
1.507GlnAsp: 1.507 ± 0.383
2.12GlnGlu: 2.12 ± 0.421
1.395GlnPhe: 1.395 ± 0.214
2.344GlnGly: 2.344 ± 0.436
0.781GlnHis: 0.781 ± 0.242
1.618GlnIle: 1.618 ± 0.264
2.232GlnLys: 2.232 ± 0.328
3.404GlnLeu: 3.404 ± 0.421
0.893GlnMet: 0.893 ± 0.227
1.451GlnAsn: 1.451 ± 0.266
1.116GlnPro: 1.116 ± 0.256
1.786GlnGln: 1.786 ± 0.321
2.232GlnArg: 2.232 ± 0.401
1.116GlnSer: 1.116 ± 0.221
1.897GlnThr: 1.897 ± 0.43
2.511GlnVal: 2.511 ± 0.333
0.558GlnTrp: 0.558 ± 0.161
1.507GlnTyr: 1.507 ± 0.283
0.0GlnXaa: 0.0 ± 0.0
Arg
3.794ArgAla: 3.794 ± 0.501
0.446ArgCys: 0.446 ± 0.191
2.399ArgAsp: 2.399 ± 0.324
3.069ArgGlu: 3.069 ± 0.457
1.451ArgPhe: 1.451 ± 0.264
2.567ArgGly: 2.567 ± 0.337
1.451ArgHis: 1.451 ± 0.306
3.013ArgIle: 3.013 ± 0.376
3.571ArgLys: 3.571 ± 0.479
4.297ArgLeu: 4.297 ± 0.625
1.395ArgMet: 1.395 ± 0.281
2.455ArgAsn: 2.455 ± 0.373
1.451ArgPro: 1.451 ± 0.325
2.455ArgGln: 2.455 ± 0.45
3.404ArgArg: 3.404 ± 0.497
3.236ArgSer: 3.236 ± 0.53
2.734ArgThr: 2.734 ± 0.396
3.85ArgVal: 3.85 ± 0.463
0.558ArgTrp: 0.558 ± 0.16
1.897ArgTyr: 1.897 ± 0.356
0.0ArgXaa: 0.0 ± 0.0
Ser
5.301SerAla: 5.301 ± 0.689
0.167SerCys: 0.167 ± 0.106
3.013SerAsp: 3.013 ± 0.421
3.404SerGlu: 3.404 ± 0.438
2.678SerPhe: 2.678 ± 0.38
5.524SerGly: 5.524 ± 0.585
1.06SerHis: 1.06 ± 0.251
4.018SerIle: 4.018 ± 0.452
4.297SerLys: 4.297 ± 0.556
4.631SerLeu: 4.631 ± 0.539
1.841SerMet: 1.841 ± 0.353
3.515SerAsn: 3.515 ± 0.441
2.902SerPro: 2.902 ± 0.45
2.065SerGln: 2.065 ± 0.276
3.181SerArg: 3.181 ± 0.381
4.799SerSer: 4.799 ± 0.822
4.464SerThr: 4.464 ± 0.712
2.902SerVal: 2.902 ± 0.357
1.451SerTrp: 1.451 ± 0.303
2.399SerTyr: 2.399 ± 0.375
0.0SerXaa: 0.0 ± 0.0
Thr
5.524ThrAla: 5.524 ± 0.8
0.614ThrCys: 0.614 ± 0.214
3.739ThrAsp: 3.739 ± 0.45
3.404ThrGlu: 3.404 ± 0.537
2.678ThrPhe: 2.678 ± 0.384
5.189ThrGly: 5.189 ± 0.547
0.893ThrHis: 0.893 ± 0.247
3.906ThrIle: 3.906 ± 0.554
3.683ThrLys: 3.683 ± 0.436
5.468ThrLeu: 5.468 ± 0.586
1.73ThrMet: 1.73 ± 0.376
2.623ThrAsn: 2.623 ± 0.33
3.739ThrPro: 3.739 ± 0.574
1.953ThrGln: 1.953 ± 0.313
2.065ThrArg: 2.065 ± 0.355
3.125ThrSer: 3.125 ± 0.469
4.408ThrThr: 4.408 ± 0.534
4.52ThrVal: 4.52 ± 0.525
1.06ThrTrp: 1.06 ± 0.335
2.12ThrTyr: 2.12 ± 0.353
0.0ThrXaa: 0.0 ± 0.0
Val
7.31ValAla: 7.31 ± 0.608
0.112ValCys: 0.112 ± 0.085
4.073ValAsp: 4.073 ± 0.447
4.073ValGlu: 4.073 ± 0.588
2.623ValPhe: 2.623 ± 0.49
4.52ValGly: 4.52 ± 0.509
1.004ValHis: 1.004 ± 0.246
4.129ValIle: 4.129 ± 0.534
4.966ValLys: 4.966 ± 0.642
5.692ValLeu: 5.692 ± 0.584
1.897ValMet: 1.897 ± 0.275
4.073ValAsn: 4.073 ± 0.462
2.734ValPro: 2.734 ± 0.409
2.176ValGln: 2.176 ± 0.414
3.627ValArg: 3.627 ± 0.454
4.743ValSer: 4.743 ± 0.6
3.236ValThr: 3.236 ± 0.346
5.134ValVal: 5.134 ± 0.568
0.837ValTrp: 0.837 ± 0.206
2.846ValTyr: 2.846 ± 0.531
0.0ValXaa: 0.0 ± 0.0
Trp
1.395TrpAla: 1.395 ± 0.344
0.112TrpCys: 0.112 ± 0.082
1.228TrpAsp: 1.228 ± 0.302
0.67TrpGlu: 0.67 ± 0.201
0.502TrpPhe: 0.502 ± 0.177
1.507TrpGly: 1.507 ± 0.286
0.335TrpHis: 0.335 ± 0.177
0.614TrpIle: 0.614 ± 0.208
0.67TrpLys: 0.67 ± 0.227
1.004TrpLeu: 1.004 ± 0.214
0.502TrpMet: 0.502 ± 0.159
0.893TrpAsn: 0.893 ± 0.252
0.223TrpPro: 0.223 ± 0.112
0.893TrpGln: 0.893 ± 0.193
0.614TrpArg: 0.614 ± 0.262
0.614TrpSer: 0.614 ± 0.211
1.116TrpThr: 1.116 ± 0.209
1.004TrpVal: 1.004 ± 0.253
0.223TrpTrp: 0.223 ± 0.131
0.446TrpTyr: 0.446 ± 0.137
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.683TyrAla: 3.683 ± 0.502
0.335TyrCys: 0.335 ± 0.148
2.344TyrAsp: 2.344 ± 0.548
1.786TyrGlu: 1.786 ± 0.385
1.618TyrPhe: 1.618 ± 0.265
2.12TyrGly: 2.12 ± 0.296
0.725TyrHis: 0.725 ± 0.228
1.953TyrIle: 1.953 ± 0.337
2.065TyrLys: 2.065 ± 0.341
1.786TyrLeu: 1.786 ± 0.336
0.893TyrMet: 0.893 ± 0.24
2.176TyrAsn: 2.176 ± 0.334
1.004TyrPro: 1.004 ± 0.258
1.06TyrGln: 1.06 ± 0.236
1.73TyrArg: 1.73 ± 0.311
2.176TyrSer: 2.176 ± 0.359
2.232TyrThr: 2.232 ± 0.35
2.344TyrVal: 2.344 ± 0.364
0.391TyrTrp: 0.391 ± 0.183
1.172TyrTyr: 1.172 ± 0.339
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (17922 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski