Rhodococcus virus RGL3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rerduovirus

Average proteome isoelectric point is 5.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9FHP4|G9FHP4_9CAUD Uncharacterized protein OS=Rhodococcus virus RGL3 OX=1109716 PE=4 SV=1
MM1 pKa = 7.26KK2 pKa = 7.83TTLEE6 pKa = 4.87LILEE10 pKa = 4.13AMILEE15 pKa = 4.69GWDD18 pKa = 3.75EE19 pKa = 4.03EE20 pKa = 4.54WGGDD24 pKa = 3.08VDD26 pKa = 5.89APTGCYY32 pKa = 9.96SRR34 pKa = 11.84LSVEE38 pKa = 5.14ASDD41 pKa = 5.37LPDD44 pKa = 3.2VMLSFGDD51 pKa = 3.75LVEE54 pKa = 4.56DD55 pKa = 3.93AEE57 pKa = 5.03EE58 pKa = 5.04GFTLDD63 pKa = 4.57ALIGYY68 pKa = 7.66WLYY71 pKa = 11.04RR72 pKa = 11.84KK73 pKa = 10.23DD74 pKa = 4.08SMGIPYY80 pKa = 9.37PEE82 pKa = 4.74RR83 pKa = 11.84YY84 pKa = 7.69EE85 pKa = 4.17TEE87 pKa = 3.5QQAIHH92 pKa = 6.98RR93 pKa = 11.84YY94 pKa = 8.76EE95 pKa = 3.96EE96 pKa = 4.47LEE98 pKa = 3.8EE99 pKa = 4.82SYY101 pKa = 10.87EE102 pKa = 3.75RR103 pKa = 11.84WYY105 pKa = 10.56DD106 pKa = 3.47EE107 pKa = 4.91DD108 pKa = 3.58EE109 pKa = 4.97LYY111 pKa = 11.14VV112 pKa = 3.55

Molecular weight: 13.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9FHN8|G9FHN8_9CAUD Uncharacterized protein OS=Rhodococcus virus RGL3 OX=1109716 PE=4 SV=1
MM1 pKa = 7.21TKK3 pKa = 10.59ARR5 pKa = 11.84TCKK8 pKa = 10.29DD9 pKa = 3.16CAAEE13 pKa = 4.85GITTKK18 pKa = 10.57RR19 pKa = 11.84PAPHH23 pKa = 7.14PGPRR27 pKa = 11.84CATHH31 pKa = 6.24HH32 pKa = 6.44RR33 pKa = 11.84AVSDD37 pKa = 3.37QRR39 pKa = 11.84RR40 pKa = 11.84ATARR44 pKa = 11.84EE45 pKa = 3.64RR46 pKa = 11.84HH47 pKa = 5.06ITTTYY52 pKa = 10.69GITEE56 pKa = 4.15AEE58 pKa = 3.99YY59 pKa = 10.33AAIYY63 pKa = 7.39EE64 pKa = 4.32AQGGSCAICQRR75 pKa = 11.84AKK77 pKa = 9.14GTGRR81 pKa = 11.84KK82 pKa = 9.08RR83 pKa = 11.84LSVDD87 pKa = 3.11HH88 pKa = 6.88DD89 pKa = 4.14HH90 pKa = 6.27EE91 pKa = 4.75TGMVRR96 pKa = 11.84GLLCSKK102 pKa = 10.42CNRR105 pKa = 11.84DD106 pKa = 3.96VLGHH110 pKa = 6.46LHH112 pKa = 7.49DD113 pKa = 5.74DD114 pKa = 2.88VDD116 pKa = 3.74AFLRR120 pKa = 11.84GAEE123 pKa = 4.17YY124 pKa = 10.66LRR126 pKa = 11.84SPPARR131 pKa = 11.84GVIGLRR137 pKa = 11.84IVPVDD142 pKa = 3.74GAPTKK147 pKa = 10.58ARR149 pKa = 11.84PAPPRR154 pKa = 11.84RR155 pKa = 11.84RR156 pKa = 11.84RR157 pKa = 11.84KK158 pKa = 9.14RR159 pKa = 11.84RR160 pKa = 11.84ARR162 pKa = 11.84KK163 pKa = 9.06SAA165 pKa = 3.06

Molecular weight: 18.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

14263

44

1178

216.1

23.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.745 ± 0.433

0.869 ± 0.153

6.471 ± 0.227

7.523 ± 0.421

3.576 ± 0.192

8.441 ± 0.347

2.138 ± 0.18

4.487 ± 0.198

3.688 ± 0.219

8.294 ± 0.337

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.342 ± 0.139

3.372 ± 0.194

4.768 ± 0.194

3.309 ± 0.155

6.24 ± 0.324

5.728 ± 0.256

6.289 ± 0.276

7.768 ± 0.212

1.851 ± 0.145

3.099 ± 0.254
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski