Arthrobacter sp. ERGS1:01

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter; unclassified Arthrobacter

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3894 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M4LC49|A0A0M4LC49_9MICC Acetyltransferase OS=Arthrobacter sp. ERGS1:01 OX=1704044 GN=AL755_14900 PE=4 SV=1
MM1 pKa = 7.13VVPGPADD8 pKa = 3.58PGGAGGNAAGLPGWVPPGPADD29 pKa = 4.19PADD32 pKa = 4.25PPPDD36 pKa = 2.99GWYY39 pKa = 10.62APLVAKK45 pKa = 10.1DD46 pKa = 3.99CQSMPGVDD54 pKa = 3.25TSGDD58 pKa = 3.25GGAITLVTAAFDD70 pKa = 3.3VCIAVTTNSADD81 pKa = 3.17AWDD84 pKa = 4.1AASAAVEE91 pKa = 4.31SLTVPSGSGCLEE103 pKa = 3.86TATFAVLGKK112 pKa = 10.33VLAYY116 pKa = 10.21HH117 pKa = 7.11LAHH120 pKa = 7.05AGTPVEE126 pKa = 4.42TVPGTGTACPLGLTGVTEE144 pKa = 4.22QNGDD148 pKa = 3.47PSAVNPQVPAPGGTEE163 pKa = 3.65LRR165 pKa = 11.84LMGRR169 pKa = 11.84FLAVTSVLVNGLSVPVSRR187 pKa = 11.84LAEE190 pKa = 3.72NHH192 pKa = 6.48FDD194 pKa = 3.65FTAPPAAVPGPVTVTAVGPDD214 pKa = 3.55GAALPGSATFTYY226 pKa = 10.06LASAGQPSTSSDD238 pKa = 2.85ASAEE242 pKa = 3.96PTTPDD247 pKa = 3.61TMGTPP252 pKa = 3.78

Molecular weight: 24.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M3T5A8|A0A0M3T5A8_9MICC Glyoxalase OS=Arthrobacter sp. ERGS1:01 OX=1704044 GN=AL755_15800 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.82GRR40 pKa = 11.84VEE42 pKa = 4.32LSAA45 pKa = 5.19

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3894

0

3894

1235914

37

2052

317.4

33.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.462 ± 0.058

0.592 ± 0.009

5.508 ± 0.033

5.171 ± 0.036

3.311 ± 0.023

9.058 ± 0.032

2.082 ± 0.019

4.549 ± 0.032

2.794 ± 0.027

10.347 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.156 ± 0.017

2.655 ± 0.027

5.313 ± 0.029

2.983 ± 0.021

6.088 ± 0.037

5.762 ± 0.028

6.127 ± 0.037

8.505 ± 0.029

1.465 ± 0.017

2.072 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski