Leadbetterella byssophila (strain DSM 17132 / JCM 16389 / KACC 11308 / NBRC 106382 / 4M15)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Leadbetterella; Leadbetterella byssophila

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3429 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E4RXX5|E4RXX5_LEAB4 Transcriptional regulator AraC family OS=Leadbetterella byssophila (strain DSM 17132 / JCM 16389 / KACC 11308 / NBRC 106382 / 4M15) OX=649349 GN=Lbys_3423 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 10.66YY3 pKa = 10.33SINPACTTANVGEE16 pKa = 4.29EE17 pKa = 4.29VVVLNADD24 pKa = 3.45SGVYY28 pKa = 10.07FSLNEE33 pKa = 3.71VGGFLWEE40 pKa = 4.51KK41 pKa = 10.2IKK43 pKa = 10.93AGPVSAEE50 pKa = 3.87DD51 pKa = 3.57LVNCVVEE58 pKa = 5.98AYY60 pKa = 8.32DD61 pKa = 4.29TDD63 pKa = 3.93EE64 pKa = 4.57PTCKK68 pKa = 10.35DD69 pKa = 3.14DD70 pKa = 3.67VLRR73 pKa = 11.84ILYY76 pKa = 9.39EE77 pKa = 4.32LINEE81 pKa = 3.97NLIFEE86 pKa = 4.53EE87 pKa = 4.43

Molecular weight: 9.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E4RZV4|E4RZV4_LEAB4 Uncharacterized protein OS=Leadbetterella byssophila (strain DSM 17132 / JCM 16389 / KACC 11308 / NBRC 106382 / 4M15) OX=649349 GN=Lbys_3598 PE=4 SV=1
MM1 pKa = 7.28NVFLRR6 pKa = 11.84KK7 pKa = 9.97SITYY11 pKa = 10.39LLLCILSLIAIVRR24 pKa = 11.84DD25 pKa = 3.72DD26 pKa = 3.86FRR28 pKa = 11.84SFSWLEE34 pKa = 3.61IAISALLLLVLLFEE48 pKa = 5.58SARR51 pKa = 11.84QLKK54 pKa = 10.34KK55 pKa = 10.22YY56 pKa = 10.14FKK58 pKa = 10.5LRR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84LDD64 pKa = 3.34LSKK67 pKa = 10.61IRR69 pKa = 4.27

Molecular weight: 8.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3429

0

3429

1198587

31

5250

349.5

39.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.806 ± 0.044

0.745 ± 0.019

5.077 ± 0.027

6.663 ± 0.043

4.979 ± 0.035

7.08 ± 0.045

1.932 ± 0.023

6.986 ± 0.036

7.199 ± 0.045

9.775 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.246 ± 0.022

5.057 ± 0.044

3.937 ± 0.024

3.628 ± 0.025

4.156 ± 0.03

6.499 ± 0.036

5.381 ± 0.064

6.392 ± 0.034

1.303 ± 0.016

4.161 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski