Tannerella forsythia (strain ATCC 43037 / JCM 10827 / CCUG 33226 / KCTC 5666 / FDC 338) (Bacteroides forsythus) 
Average proteome isoelectric point is 6.96 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 2978 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|G8UK42|G8UK42_TANFA Uncharacterized protein OS=Tannerella forsythia (strain ATCC 43037 / JCM 10827 / CCUG 33226 / KCTC 5666 / FDC 338) OX=203275 GN=BFO_0346 PE=4 SV=1MM1 pKa = 7.48  KK2 pKa = 10.09  KK3 pKa = 10.1  YY4 pKa = 10.52  RR5 pKa = 11.84  CIVCDD10 pKa = 3.71  YY11 pKa = 10.44  IYY13 pKa = 10.89  DD14 pKa = 4.07  PVEE17 pKa = 4.26  GDD19 pKa = 3.5  PDD21 pKa = 3.9  GGIDD25 pKa = 3.74  PGTAFEE31 pKa = 6.02  DD32 pKa = 4.23  IPDD35 pKa = 3.74  DD36 pKa = 4.06  WVCPLCGVGKK46 pKa = 10.3  DD47 pKa = 3.4  DD48 pKa = 4.99  FEE50 pKa = 4.51  PVEE53 pKa = 4.05  
 5.9 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.788 
IPC2_protein 3.668 
IPC_protein 3.617 
Toseland    3.414 
ProMoST     3.783 
Dawson      3.643 
Bjellqvist  3.884 
Wikipedia   3.617 
Rodwell     3.452 
Grimsley    3.325 
Solomon     3.605 
Lehninger   3.567 
Nozaki      3.783 
DTASelect   4.024 
Thurlkill   3.49 
EMBOSS      3.63 
Sillero     3.757 
Patrickios  0.401 
IPC_peptide 3.605 
IPC2_peptide  3.719 
IPC2.peptide.svr19  3.744 
 Protein with the highest isoelectric point: 
>tr|G8UKB5|G8UKB5_TANFA Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase OS=Tannerella forsythia (strain ATCC 43037 / JCM 10827 / CCUG 33226 / KCTC 5666 / FDC 338) OX=203275 GN=BFO_1375 PE=4 SV=1MM1 pKa = 7.79  RR2 pKa = 11.84  PMIKK6 pKa = 8.66  KK7 pKa = 8.31  TKK9 pKa = 8.39  RR10 pKa = 11.84  QQLGRR15 pKa = 11.84  VLNIALNVFNGISMIIIGFLLMRR38 pKa = 11.84  VFVFGSS44 pKa = 3.28  
 5.05 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.352 
IPC2_protein 10.76 
IPC_protein 12.325 
Toseland    12.486 
ProMoST     12.983 
Dawson      12.501 
Bjellqvist  12.486 
Wikipedia   12.969 
Rodwell     12.281 
Grimsley    12.53 
Solomon     12.983 
Lehninger   12.881 
Nozaki      12.486 
DTASelect   12.486 
Thurlkill   12.486 
EMBOSS      12.983 
Sillero     12.486 
Patrickios  12.047 
IPC_peptide 12.983 
IPC2_peptide  11.974 
IPC2.peptide.svr19  9.065 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        2978 
0
2978 
978559
37
3058
328.6
37.18
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        7.098 ± 0.044
1.2 ± 0.019
5.418 ± 0.03
6.416 ± 0.045
4.666 ± 0.035
6.837 ± 0.044
2.086 ± 0.022
6.742 ± 0.039
6.282 ± 0.045
9.093 ± 0.048
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.752 ± 0.022
4.693 ± 0.039
3.888 ± 0.027
3.348 ± 0.027
5.563 ± 0.038
6.107 ± 0.039
5.775 ± 0.044
6.394 ± 0.037
1.234 ± 0.017
4.408 ± 0.03
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here