Streptococcus phage Javan47

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D6ADW2|A0A4D6ADW2_9CAUD Replicative DNA helicase OS=Streptococcus phage Javan47 OX=2548188 GN=Javan47_0021 PE=4 SV=1
MM1 pKa = 8.02IEE3 pKa = 4.62VEE5 pKa = 4.17RR6 pKa = 11.84QPKK9 pKa = 7.79NTYY12 pKa = 10.08SRR14 pKa = 11.84IRR16 pKa = 11.84CSNNIYY22 pKa = 10.88DD23 pKa = 4.91EE24 pKa = 4.22IANIANEE31 pKa = 4.13CDD33 pKa = 3.44LTLKK37 pKa = 10.53EE38 pKa = 4.19VTDD41 pKa = 3.79ALLNYY46 pKa = 9.81GLANAQIISSEE57 pKa = 4.17KK58 pKa = 10.31VVTEE62 pKa = 4.05SKK64 pKa = 10.8LVIGDD69 pKa = 4.21FDD71 pKa = 4.23NDD73 pKa = 3.38NNNN76 pKa = 3.07

Molecular weight:
8.57 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D6ADM4|A0A4D6ADM4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan47 OX=2548188 GN=Javan47_0047 PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84GLDD5 pKa = 3.09RR6 pKa = 11.84FLRR9 pKa = 11.84SVEE12 pKa = 4.11RR13 pKa = 11.84KK14 pKa = 9.2QKK16 pKa = 9.01SARR19 pKa = 11.84IAVDD23 pKa = 3.36KK24 pKa = 10.62EE25 pKa = 4.09LSKK28 pKa = 10.9SAARR32 pKa = 11.84IEE34 pKa = 4.15RR35 pKa = 11.84QAKK38 pKa = 9.19ILAPVDD44 pKa = 4.04TTWLRR49 pKa = 11.84SQIYY53 pKa = 10.27NEE55 pKa = 4.04QQKK58 pKa = 10.03LLHH61 pKa = 5.87YY62 pKa = 9.79RR63 pKa = 11.84VVSPALYY70 pKa = 10.27SIYY73 pKa = 10.79LEE75 pKa = 4.28LGTRR79 pKa = 11.84KK80 pKa = 8.87MEE82 pKa = 4.32AQPFLDD88 pKa = 3.7PALRR92 pKa = 11.84KK93 pKa = 8.48EE94 pKa = 4.46WPVLMANLKK103 pKa = 10.77KK104 pKa = 9.96MFKK107 pKa = 10.29RR108 pKa = 3.74

Molecular weight:
12.76 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12306

38

1408

223.7

25.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.525 ± 0.828

0.496 ± 0.108

6.208 ± 0.506

6.988 ± 0.633

3.608 ± 0.169

5.924 ± 0.396

1.154 ± 0.144

7.728 ± 0.393

8.207 ± 0.588

8.216 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.405 ± 0.266

6.355 ± 0.4

2.82 ± 0.26

4.567 ± 0.268

4.136 ± 0.378

6.55 ± 0.705

6.363 ± 0.343

6.013 ± 0.248

1.243 ± 0.158

3.494 ± 0.357

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski