Pseudomonas fluorescens (strain F113)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; Pseudomonas fluorescens group; Pseudomonas fluorescens

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5837 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|G8PZR1|G8PZR1_PSEF3 Glyoxalase family protein superfamily OS=Pseudomonas fluorescens (strain F113) OX=1114970 GN=PSF113_2727 PE=4 SV=1
MM1 pKa = 7.56AALIGIVSKK10 pKa = 11.2VVGQVFAEE18 pKa = 4.09AAGGLRR24 pKa = 11.84RR25 pKa = 11.84PLVEE29 pKa = 4.19GDD31 pKa = 3.02RR32 pKa = 11.84LYY34 pKa = 11.13AGEE37 pKa = 4.25HH38 pKa = 5.9LVTGAEE44 pKa = 4.3GAVAVHH50 pKa = 5.68MQNGQTLTLGRR61 pKa = 11.84EE62 pKa = 4.36SNLTLTPQLLANHH75 pKa = 7.11APHH78 pKa = 7.02VDD80 pKa = 3.68TPDD83 pKa = 3.08AAAPSDD89 pKa = 3.73AQLTDD94 pKa = 3.49VQKK97 pKa = 10.75LQQAIAAGADD107 pKa = 3.42PTQTGEE113 pKa = 3.82ATAAGPEE120 pKa = 4.21GGNPGGVGGGHH131 pKa = 5.85SFVLLEE137 pKa = 4.15EE138 pKa = 4.42VGGEE142 pKa = 4.0VDD144 pKa = 3.89PLIGFPTAGFNGIPEE159 pKa = 4.33FPLLRR164 pKa = 11.84LAGDD168 pKa = 4.19PDD170 pKa = 3.72NSGDD174 pKa = 3.91DD175 pKa = 3.44ATVPPVTPEE184 pKa = 4.01TPDD187 pKa = 3.32NPVTLDD193 pKa = 3.44GVNVEE198 pKa = 4.27GGEE201 pKa = 4.18LTTNEE206 pKa = 4.18ANLADD211 pKa = 4.47GSASNPGALVQSGTFTVSAPDD232 pKa = 3.77GLSSLSIGGINVISGGVPAGFPQTITSALGNTLTITGYY270 pKa = 10.97DD271 pKa = 3.57PATGVVSYY279 pKa = 9.84TYY281 pKa = 10.63TLTDD285 pKa = 3.63NEE287 pKa = 4.34THH289 pKa = 6.8PAGGGANSITEE300 pKa = 4.14QFPVVAVDD308 pKa = 3.54TDD310 pKa = 3.44GDD312 pKa = 4.25TATGTLDD319 pKa = 3.87VNITDD324 pKa = 5.29DD325 pKa = 3.83VPQAIDD331 pKa = 3.76DD332 pKa = 4.04SHH334 pKa = 8.14ANTASEE340 pKa = 4.3TLVTLTGNVLPNDD353 pKa = 3.65HH354 pKa = 6.54QGADD358 pKa = 4.52RR359 pKa = 11.84IPTGPDD365 pKa = 2.56SGPIIGGTFTGTYY378 pKa = 7.22GTLVLNPNGTYY389 pKa = 9.73IYY391 pKa = 9.75TLNTSDD397 pKa = 3.65PQFVALHH404 pKa = 6.29GGGSGTEE411 pKa = 4.06TFTYY415 pKa = 10.31TLTDD419 pKa = 3.44ADD421 pKa = 4.77GDD423 pKa = 3.98TSTANLVLQVHH434 pKa = 6.33NNDD437 pKa = 3.98DD438 pKa = 3.89PVVLVGLDD446 pKa = 3.47AEE448 pKa = 4.66GGEE451 pKa = 5.36LSLQEE456 pKa = 4.79KK457 pKa = 10.07NLSDD461 pKa = 4.1GSNPDD466 pKa = 3.26ASALTQSGTFTVTALDD482 pKa = 4.08GVQTLSVGGINVVTGGVAAGFPQSITTALGNTLTITGFNAATGVVSYY529 pKa = 10.95SYY531 pKa = 10.27TLLDD535 pKa = 4.73NEE537 pKa = 4.59AHH539 pKa = 6.61PNANGANSVSEE550 pKa = 3.94QFAVVVTDD558 pKa = 5.35DD559 pKa = 4.31NGTTANGNLDD569 pKa = 3.66VNIVDD574 pKa = 5.11DD575 pKa = 4.5LPKK578 pKa = 10.85AVDD581 pKa = 4.02DD582 pKa = 4.9SNASTASEE590 pKa = 4.38TNLTLTGSVLTNDD603 pKa = 3.68TQGADD608 pKa = 3.54HH609 pKa = 6.49VASGPITPGTFTGTYY624 pKa = 7.23GTLVLNADD632 pKa = 3.56GSYY635 pKa = 10.19TYY637 pKa = 10.56TLNTADD643 pKa = 4.52ADD645 pKa = 4.28FKK647 pKa = 11.26GLHH650 pKa = 6.14GGGNGSEE657 pKa = 4.21TFTYY661 pKa = 10.51TLTDD665 pKa = 3.44ADD667 pKa = 4.77GDD669 pKa = 3.98TSTANLVLQVHH680 pKa = 6.32NNDD683 pKa = 3.95DD684 pKa = 3.82PVIITGLDD692 pKa = 3.43TEE694 pKa = 4.84GGEE697 pKa = 4.73LTIQEE702 pKa = 4.81KK703 pKa = 10.51NLSDD707 pKa = 3.9GSSPDD712 pKa = 3.37ASALTQSGTFIVTALDD728 pKa = 3.94GVQTLSVGGINVVTGGVAAGFPQSITTALGNTLTITGFNAATGVVSYY775 pKa = 10.95SYY777 pKa = 10.27TLLDD781 pKa = 4.73NEE783 pKa = 4.59AHH785 pKa = 6.61PNANGANSLSEE796 pKa = 3.87QFAVVVTDD804 pKa = 5.35DD805 pKa = 4.31NGTTANGNLDD815 pKa = 3.66VNIVDD820 pKa = 5.29DD821 pKa = 5.21LPTAHH826 pKa = 7.41ADD828 pKa = 3.44SASVDD833 pKa = 3.32EE834 pKa = 5.14GGTVSGNVLNNDD846 pKa = 3.19EE847 pKa = 4.87GGADD851 pKa = 3.72GPAASGAVIGVRR863 pKa = 11.84AGNDD867 pKa = 2.99TSTPAIGGLNTQINGTYY884 pKa = 10.63GYY886 pKa = 8.42LTLDD890 pKa = 3.37ANGNAVYY897 pKa = 10.14HH898 pKa = 6.15SNPNTVSAPGATDD911 pKa = 3.07VFTYY915 pKa = 7.69TVRR918 pKa = 11.84DD919 pKa = 3.44ADD921 pKa = 3.88GDD923 pKa = 3.89EE924 pKa = 4.27STTTITIDD932 pKa = 3.25VHH934 pKa = 6.52DD935 pKa = 4.63VCLVATPDD943 pKa = 3.57QEE945 pKa = 3.72ISVYY949 pKa = 10.56EE950 pKa = 4.0KK951 pKa = 11.09ALDD954 pKa = 4.02LNQDD958 pKa = 3.77GQDD961 pKa = 3.77LAPGTVTGSAPSATSEE977 pKa = 4.17TASGSLVGSISGAVGAVTFALVGNANGAYY1006 pKa = 9.61GQLSLQPDD1014 pKa = 3.98GSYY1017 pKa = 10.19TYY1019 pKa = 10.41TLTSPATTTPHH1030 pKa = 6.98ANDD1033 pKa = 3.96GPNVLSEE1040 pKa = 4.3SFTYY1044 pKa = 10.34QATDD1048 pKa = 3.04SLGNTVTSTIVIDD1061 pKa = 4.47IVDD1064 pKa = 4.47DD1065 pKa = 4.01VPNAHH1070 pKa = 7.28ADD1072 pKa = 3.52FASVVEE1078 pKa = 4.28GGTVNGNVLDD1088 pKa = 4.09NDD1090 pKa = 3.75VLGADD1095 pKa = 3.54GGMVVGVRR1103 pKa = 11.84AGNDD1107 pKa = 3.21TSNPAVGGLNTQVNGTYY1124 pKa = 10.69GYY1126 pKa = 8.5LTLDD1130 pKa = 3.37ANGNAVYY1137 pKa = 10.14HH1138 pKa = 6.15SNPNTVSAPGATDD1151 pKa = 3.07VFTYY1155 pKa = 7.69TVRR1158 pKa = 11.84DD1159 pKa = 3.44ADD1161 pKa = 3.88GDD1163 pKa = 3.89EE1164 pKa = 4.27STTTITIDD1172 pKa = 3.25VHH1174 pKa = 6.52DD1175 pKa = 4.63VCLVATPDD1183 pKa = 3.57QEE1185 pKa = 3.72ISVYY1189 pKa = 10.56EE1190 pKa = 4.0KK1191 pKa = 11.09ALDD1194 pKa = 4.02LNQDD1198 pKa = 3.77GQDD1201 pKa = 3.77LAPGTVTGSAPSATSEE1217 pKa = 4.17TASGSLVGSISGAVGAVTFALVGNANGAYY1246 pKa = 9.61GQLSLQPDD1254 pKa = 3.98GSYY1257 pKa = 10.19TYY1259 pKa = 10.41TLTSPATTTPHH1270 pKa = 6.98ANDD1273 pKa = 3.96GPNVLSEE1280 pKa = 4.3SFTYY1284 pKa = 10.34QATDD1288 pKa = 3.04SLGNTVTSTIVIDD1301 pKa = 4.47IVDD1304 pKa = 4.47DD1305 pKa = 4.01VPNAHH1310 pKa = 7.28ADD1312 pKa = 3.52FASVVEE1318 pKa = 4.28GGTVNGNVLDD1328 pKa = 4.09NDD1330 pKa = 3.75VLGADD1335 pKa = 3.6GGMVIGVRR1343 pKa = 11.84AGNDD1347 pKa = 3.2TSNPAVGGLNTQINGTYY1364 pKa = 10.63GYY1366 pKa = 8.42LTLDD1370 pKa = 3.37ANGNAVYY1377 pKa = 10.17HH1378 pKa = 6.18SNPDD1382 pKa = 3.18AVGAAGATDD1391 pKa = 3.31VFTYY1395 pKa = 7.69TVRR1398 pKa = 11.84DD1399 pKa = 3.44ADD1401 pKa = 3.88GDD1403 pKa = 3.89EE1404 pKa = 4.35STTTVTIDD1412 pKa = 3.11VHH1414 pKa = 6.09NSCLIVEE1421 pKa = 4.51TDD1423 pKa = 3.07HH1424 pKa = 7.88DD1425 pKa = 3.61ITVYY1429 pKa = 10.73EE1430 pKa = 4.43KK1431 pKa = 11.06ALDD1434 pKa = 4.09LNQDD1438 pKa = 3.77GQDD1441 pKa = 3.77LAPGTVTGSEE1451 pKa = 4.0PSATGEE1457 pKa = 4.13TASGSLVGSVSGATGAVTFTLVGNATGAYY1486 pKa = 8.53GQLLLHH1492 pKa = 7.18PDD1494 pKa = 3.23GSYY1497 pKa = 10.24TYY1499 pKa = 9.8TLTSPATTTPHH1510 pKa = 7.16ANDD1513 pKa = 4.01GPNALSEE1520 pKa = 4.45SFTYY1524 pKa = 10.33QATDD1528 pKa = 3.0ALGNSTTGSLVVSIVDD1544 pKa = 3.84DD1545 pKa = 3.93VPKK1548 pKa = 10.7AVASEE1553 pKa = 4.22RR1554 pKa = 11.84SVTAVEE1560 pKa = 4.07IDD1562 pKa = 3.66TNLLIVLDD1570 pKa = 4.28VSGSMKK1576 pKa = 10.53DD1577 pKa = 3.38DD1578 pKa = 3.81SGVSGLSRR1586 pKa = 11.84MEE1588 pKa = 4.09LAKK1591 pKa = 10.44QAISALLDD1599 pKa = 3.63KK1600 pKa = 10.72YY1601 pKa = 11.63DD1602 pKa = 4.35DD1603 pKa = 3.8MGDD1606 pKa = 3.42VKK1608 pKa = 11.12VQLVTFSGSATDD1620 pKa = 5.44QSTLWVDD1627 pKa = 3.28VATAKK1632 pKa = 9.99TLISALSAHH1641 pKa = 7.0GDD1643 pKa = 3.62TNYY1646 pKa = 11.0DD1647 pKa = 3.11AAVAVAKK1654 pKa = 9.21TAFANPGQLTGAQNVGYY1671 pKa = 9.32FFSDD1675 pKa = 3.77GKK1677 pKa = 8.61PTLGEE1682 pKa = 3.73IGSADD1687 pKa = 3.36EE1688 pKa = 4.51AAWKK1692 pKa = 10.66AFLDD1696 pKa = 3.71ANGIKK1701 pKa = 10.28NYY1703 pKa = 10.7AIGLGDD1709 pKa = 3.76GVSNTHH1715 pKa = 7.21LDD1717 pKa = 3.41PLAYY1721 pKa = 10.19DD1722 pKa = 4.67GSAHH1726 pKa = 6.29TNTNAVVVTDD1736 pKa = 4.52LNQLNSVLSDD1746 pKa = 3.56TVQGAPVTGNLLGEE1760 pKa = 4.6GGSFGADD1767 pKa = 2.58GGFIKK1772 pKa = 10.79SLVVDD1777 pKa = 4.58GTTYY1781 pKa = 10.82SYY1783 pKa = 11.69DD1784 pKa = 3.28PAANGGHH1791 pKa = 6.42GALTASGGLNHH1802 pKa = 7.24GSFNTATNTLSIATDD1817 pKa = 3.31HH1818 pKa = 7.55DD1819 pKa = 3.72GTLVVNLDD1827 pKa = 3.49TGAFSYY1833 pKa = 10.3TSQTATSTLITEE1845 pKa = 4.94HH1846 pKa = 6.71IGYY1849 pKa = 7.54TVSDD1853 pKa = 3.81NDD1855 pKa = 3.87GDD1857 pKa = 4.17LASSNLVINVVPNSPPIAVDD1877 pKa = 3.84DD1878 pKa = 4.75NIITNVLSGNIVIPGEE1894 pKa = 4.02LLLGNDD1900 pKa = 3.64SDD1902 pKa = 5.05VNGDD1906 pKa = 4.27PLTASPTSFNTGWTAKK1922 pKa = 10.54GGDD1925 pKa = 3.87FTGSTGTASFTGNNVQSINLSRR1947 pKa = 11.84SAFVANTASMTAMLVVSGALGMVSNNNANDD1977 pKa = 3.63EE1978 pKa = 4.06DD1979 pKa = 4.19RR1980 pKa = 11.84LNISLKK1986 pKa = 10.4QGEE1989 pKa = 5.02TLTLDD1994 pKa = 3.72HH1995 pKa = 6.73NLSAGRR2001 pKa = 11.84IAMEE2005 pKa = 3.81YY2006 pKa = 9.91SLNGGPFIAINDD2018 pKa = 4.27GASFTAAADD2027 pKa = 3.55GNYY2030 pKa = 9.4QIHH2033 pKa = 6.96VINIANPGGGNSNAAEE2049 pKa = 4.24NYY2051 pKa = 9.88QLILTVDD2058 pKa = 3.37YY2059 pKa = 11.39AGAQDD2064 pKa = 4.01KK2065 pKa = 9.94TPDD2068 pKa = 3.34YY2069 pKa = 9.81HH2070 pKa = 8.77GSYY2073 pKa = 8.77TASDD2077 pKa = 3.33NHH2079 pKa = 6.58GGSDD2083 pKa = 3.55SASVDD2088 pKa = 2.75ISYY2091 pKa = 10.68QAGHH2095 pKa = 6.53TLTGTSGDD2103 pKa = 3.88DD2104 pKa = 3.28VLVAGNGDD2112 pKa = 3.59NLLNAGDD2119 pKa = 4.26GNDD2122 pKa = 3.46ILSAGSGNNTLHH2134 pKa = 7.02GEE2136 pKa = 4.19AGDD2139 pKa = 3.96DD2140 pKa = 3.91LLYY2143 pKa = 11.17SGAGNDD2149 pKa = 4.36LLDD2152 pKa = 4.53GGTGNDD2158 pKa = 3.47TASYY2162 pKa = 10.05AHH2164 pKa = 6.97ATTGVTVNLGLLAAQNTLGAGTDD2187 pKa = 3.46TLAGIEE2193 pKa = 4.14NLMGSNFNDD2202 pKa = 3.63TLIGDD2207 pKa = 4.45GASNRR2212 pKa = 11.84INGGLGHH2219 pKa = 7.31DD2220 pKa = 4.01VLNGGGGDD2228 pKa = 3.91DD2229 pKa = 4.69LLIGGLGNNTLTGGSGADD2247 pKa = 3.36TFQWQAGNSGHH2258 pKa = 7.31DD2259 pKa = 4.0VITDD2263 pKa = 3.87FAPGVDD2269 pKa = 4.91KK2270 pKa = 11.41LDD2272 pKa = 4.61LSQLLQGEE2280 pKa = 4.45NGSAASLDD2288 pKa = 4.18DD2289 pKa = 3.73YY2290 pKa = 11.72LHH2292 pKa = 6.68FSVSGSGASVVTSIDD2307 pKa = 3.02VSAIAGATPNQTIDD2321 pKa = 3.45LTGVNLASHH2330 pKa = 6.36YY2331 pKa = 10.76GVTPGAGGIIGGADD2345 pKa = 3.06TATIINGMLNDD2356 pKa = 4.31HH2357 pKa = 6.08SLKK2360 pKa = 10.51VDD2362 pKa = 3.57TVV2364 pKa = 3.29

Molecular weight:
237.4 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|G8Q7Q1|G8Q7Q1_PSEF3 Sulfate-transport transmembrane protein abc transporter OS=Pseudomonas fluorescens (strain F113) OX=1114970 GN=PSF113_3313 PE=3 SV=1
MM1 pKa = 7.12TRR3 pKa = 11.84FFRR6 pKa = 11.84PHH8 pKa = 6.65ALFSHH13 pKa = 6.55ACVHH17 pKa = 5.66GWVLIGLLHH26 pKa = 6.47SASAMAQPDD35 pKa = 3.66VLQDD39 pKa = 3.62KK40 pKa = 9.81VRR42 pKa = 11.84HH43 pKa = 6.31LIAQRR48 pKa = 11.84LPPRR52 pKa = 11.84SQQLQIDD59 pKa = 4.12IGQPAARR66 pKa = 11.84ISACEE71 pKa = 3.84SPLPYY76 pKa = 10.05LVHH79 pKa = 7.09PEE81 pKa = 3.75QNVFGRR87 pKa = 11.84VAVGVRR93 pKa = 11.84CEE95 pKa = 4.0GDD97 pKa = 3.21EE98 pKa = 4.24GVAGYY103 pKa = 9.6LQVSVRR109 pKa = 11.84AVGDD113 pKa = 3.64YY114 pKa = 10.6VISRR118 pKa = 11.84QRR120 pKa = 11.84IAAGDD125 pKa = 3.82VVKK128 pKa = 11.22ADD130 pKa = 3.31MLEE133 pKa = 4.16IKK135 pKa = 10.37RR136 pKa = 11.84GPLEE140 pKa = 4.23RR141 pKa = 11.84LPKK144 pKa = 10.63GSVLKK149 pKa = 10.5PEE151 pKa = 4.14QVLGRR156 pKa = 11.84QASRR160 pKa = 11.84SFSRR164 pKa = 11.84GAALALNNFRR174 pKa = 11.84EE175 pKa = 4.06RR176 pKa = 11.84WLVQRR181 pKa = 11.84NQRR184 pKa = 11.84VVLQARR190 pKa = 11.84GAGFTIRR197 pKa = 11.84RR198 pKa = 11.84DD199 pKa = 3.46GKK201 pKa = 10.89ALDD204 pKa = 3.93NGSLGSSIRR213 pKa = 11.84VLSTDD218 pKa = 3.1GKK220 pKa = 10.57LLNAQVVGQNEE231 pKa = 4.16LLLRR235 pKa = 11.84YY236 pKa = 9.15

Molecular weight:
25.97 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5837

0

5837

1970731

24

5414

337.6

37.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.834 ± 0.037

0.989 ± 0.011

5.422 ± 0.022

5.609 ± 0.029

3.673 ± 0.024

7.963 ± 0.032

2.252 ± 0.017

4.837 ± 0.023

3.416 ± 0.027

11.718 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.327 ± 0.016

3.102 ± 0.027

4.882 ± 0.024

4.605 ± 0.025

6.31 ± 0.031

5.879 ± 0.025

5.057 ± 0.049

7.167 ± 0.023

1.43 ± 0.013

2.53 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski