Tumebacillus avium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Alicyclobacillaceae; Tumebacillus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4330 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y0IHI9|A0A1Y0IHI9_9BACL Uncharacterized protein OS=Tumebacillus avium OX=1903704 GN=CBW65_01670 PE=4 SV=1
MM1 pKa = 7.65DD2 pKa = 3.82VVMKK6 pKa = 10.65PEE8 pKa = 3.77LRR10 pKa = 11.84TNGGEE15 pKa = 4.2TTSIYY20 pKa = 11.09YY21 pKa = 9.83NGEE24 pKa = 3.82WAGDD28 pKa = 3.5LYY30 pKa = 11.25LVYY33 pKa = 10.5RR34 pKa = 11.84EE35 pKa = 4.26GDD37 pKa = 3.74SLTGTMQIDD46 pKa = 3.55TRR48 pKa = 11.84RR49 pKa = 11.84VEE51 pKa = 4.36EE52 pKa = 4.24NEE54 pKa = 3.81LEE56 pKa = 4.33YY57 pKa = 10.8VTDD60 pKa = 3.63EE61 pKa = 3.67VRR63 pKa = 11.84TYY65 pKa = 10.39VQHH68 pKa = 7.03LSSALNVQEE77 pKa = 4.5SSVVMMYY84 pKa = 11.28GDD86 pKa = 3.39ISSVVEE92 pKa = 4.14MEE94 pKa = 4.06PMDD97 pKa = 5.0MISEE101 pKa = 4.43DD102 pKa = 3.83DD103 pKa = 3.97VEE105 pKa = 4.64ILLTEE110 pKa = 4.1EE111 pKa = 4.52DD112 pKa = 4.04EE113 pKa = 5.45LYY115 pKa = 10.97EE116 pKa = 5.97DD117 pKa = 5.39DD118 pKa = 4.54AFEE121 pKa = 4.17MADD124 pKa = 5.44DD125 pKa = 4.46EE126 pKa = 4.71DD127 pKa = 6.21CEE129 pKa = 4.8DD130 pKa = 4.15EE131 pKa = 5.47EE132 pKa = 5.94LFADD136 pKa = 4.11DD137 pKa = 4.6DD138 pKa = 4.54VYY140 pKa = 11.86YY141 pKa = 10.74EE142 pKa = 5.08DD143 pKa = 5.96DD144 pKa = 3.96PGFYY148 pKa = 10.29AAAEE152 pKa = 4.27VEE154 pKa = 4.31EE155 pKa = 4.55EE156 pKa = 4.57PYY158 pKa = 10.46HH159 pKa = 7.31LSVVFQDD166 pKa = 4.48GEE168 pKa = 4.11HH169 pKa = 6.07TKK171 pKa = 10.75YY172 pKa = 10.19QLHH175 pKa = 7.17DD176 pKa = 4.11DD177 pKa = 3.65EE178 pKa = 6.01HH179 pKa = 6.16RR180 pKa = 11.84TLGLVSVDD188 pKa = 3.84EE189 pKa = 4.65IGSNVSGRR197 pKa = 11.84VEE199 pKa = 4.48FWSDD203 pKa = 2.83MDD205 pKa = 5.51DD206 pKa = 6.35DD207 pKa = 4.31EE208 pKa = 4.73TTDD211 pKa = 2.96IARR214 pKa = 11.84ILARR218 pKa = 11.84EE219 pKa = 3.96FSEE222 pKa = 4.13TDD224 pKa = 3.03AEE226 pKa = 4.69SISFTMNYY234 pKa = 9.31QDD236 pKa = 3.42HH237 pKa = 6.96HH238 pKa = 8.51LGDD241 pKa = 4.05FQLEE245 pKa = 4.17RR246 pKa = 11.84RR247 pKa = 11.84DD248 pKa = 3.95LMMM251 pKa = 5.85

Molecular weight: 29.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y0IKZ2|A0A1Y0IKZ2_9BACL Uncharacterized protein OS=Tumebacillus avium OX=1903704 GN=CBW65_06125 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.52RR3 pKa = 11.84TFQPNVRR10 pKa = 11.84KK11 pKa = 9.76RR12 pKa = 11.84KK13 pKa = 8.65KK14 pKa = 8.47VHH16 pKa = 5.5GFRR19 pKa = 11.84TRR21 pKa = 11.84MASKK25 pKa = 10.07NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4330

0

4330

1477804

31

8242

341.3

37.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.079 ± 0.048

0.747 ± 0.011

5.205 ± 0.028

7.095 ± 0.036

3.99 ± 0.025

7.583 ± 0.033

2.212 ± 0.016

5.536 ± 0.042

4.819 ± 0.054

10.871 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.642 ± 0.022

3.322 ± 0.033

4.222 ± 0.032

4.365 ± 0.031

5.339 ± 0.035

5.316 ± 0.029

5.661 ± 0.045

7.599 ± 0.029

1.178 ± 0.013

3.22 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski