Methylomusa anaerophila

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Sporomusaceae; Methylomusa

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4152 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A348AKX5|A0A348AKX5_9FIRM Uncharacterized protein OS=Methylomusa anaerophila OX=1930071 GN=MAMMFC1_02407 PE=4 SV=1
MM1 pKa = 7.47ALSTTTLTVAEE12 pKa = 5.21VITKK16 pKa = 9.9AASTDD21 pKa = 2.79SWVIYY26 pKa = 10.63GNTGGEE32 pKa = 4.4NITGSAFADD41 pKa = 3.64TLYY44 pKa = 11.4GLAGADD50 pKa = 3.43TLVGGNGDD58 pKa = 3.93DD59 pKa = 5.07LIVGGTGNDD68 pKa = 3.74TLTGGADD75 pKa = 3.76DD76 pKa = 3.66DD77 pKa = 4.3TFRR80 pKa = 11.84YY81 pKa = 10.22SYY83 pKa = 11.71GDD85 pKa = 3.77GNDD88 pKa = 3.89SIADD92 pKa = 3.67FGVATDD98 pKa = 3.48VDD100 pKa = 3.69ILQFTNIALSNISFNALTAGAAQNITMTGGASIRR134 pKa = 11.84ITNDD138 pKa = 2.21ATANNGNMKK147 pKa = 10.48VITSDD152 pKa = 3.25KK153 pKa = 10.24TFKK156 pKa = 10.88LYY158 pKa = 10.79LDD160 pKa = 3.86NDD162 pKa = 4.21GAPGVVGGSSLADD175 pKa = 3.61FVLGGIGGGSTLIGGTAGADD195 pKa = 3.54TLQGQAGSAGDD206 pKa = 3.7VYY208 pKa = 11.0VYY210 pKa = 10.6RR211 pKa = 11.84SDD213 pKa = 3.66LAKK216 pKa = 10.97VNALGANDD224 pKa = 4.0TLSAAVLSSDD234 pKa = 3.04GTTANNTAVEE244 pKa = 4.11INLYY248 pKa = 7.09DD249 pKa = 3.51TKK251 pKa = 10.63YY252 pKa = 8.68TGVEE256 pKa = 3.99NVVGGGGRR264 pKa = 11.84DD265 pKa = 3.55TLRR268 pKa = 11.84GSSLAEE274 pKa = 3.88TLDD277 pKa = 3.74GGNGGDD283 pKa = 4.22ILWGGAGNDD292 pKa = 3.97SLTGGAGNDD301 pKa = 3.87TYY303 pKa = 11.24WFGTGDD309 pKa = 3.76GLDD312 pKa = 3.84SIVAIGGGGADD323 pKa = 3.78GATDD327 pKa = 3.54ADD329 pKa = 3.98VYY331 pKa = 11.52NFYY334 pKa = 11.2DD335 pKa = 3.52STFSDD340 pKa = 4.6LSFAYY345 pKa = 10.33NGADD349 pKa = 2.98LVVTVGPVTNYY360 pKa = 10.19DD361 pKa = 3.44ALNIQAYY368 pKa = 10.32NNAATNGAKK377 pKa = 9.86IFQTSDD383 pKa = 2.76LTFRR387 pKa = 11.84LLASSAGPIPTGTTAVEE404 pKa = 4.0YY405 pKa = 11.2VRR407 pKa = 11.84DD408 pKa = 3.91FVGAGGVPLLDD419 pKa = 4.18GGGGADD425 pKa = 3.26TVVGGDD431 pKa = 3.3AGNTFAYY438 pKa = 10.44YY439 pKa = 10.97ADD441 pKa = 3.39TARR444 pKa = 11.84YY445 pKa = 8.96IGGSSTLDD453 pKa = 3.16VLTAATSADD462 pKa = 3.12GVEE465 pKa = 3.93INLYY469 pKa = 6.82DD470 pKa = 3.61TKK472 pKa = 11.28YY473 pKa = 10.89SGIEE477 pKa = 3.87KK478 pKa = 8.83VTGSTHH484 pKa = 7.24ADD486 pKa = 3.52TLRR489 pKa = 11.84GTSLADD495 pKa = 3.22SLTGGGGADD504 pKa = 3.99NLWGGAGNDD513 pKa = 4.03TLTGTAGASDD523 pKa = 4.41TYY525 pKa = 11.08WFGTTDD531 pKa = 3.5GNDD534 pKa = 3.47TITAAGADD542 pKa = 3.29ASDD545 pKa = 4.16YY546 pKa = 11.17IFLYY550 pKa = 10.65DD551 pKa = 4.15VSDD554 pKa = 3.47AHH556 pKa = 6.96AQITSMGIEE565 pKa = 4.13GADD568 pKa = 3.28LVLHH572 pKa = 5.86FTGGAALTIAGWTPGGSANRR592 pKa = 11.84FRR594 pKa = 11.84FEE596 pKa = 4.17GNEE599 pKa = 4.01FTWQITDD606 pKa = 3.51PTAANAWARR615 pKa = 11.84VV616 pKa = 3.5

Molecular weight: 61.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A348AHW1|A0A348AHW1_9FIRM 34 kDa membrane antigen OS=Methylomusa anaerophila OX=1930071 GN=tpd PE=3 SV=1
MM1 pKa = 6.98SLKK4 pKa = 10.03YY5 pKa = 10.32GKK7 pKa = 9.42YY8 pKa = 8.42VHH10 pKa = 7.09PIVCQTVCQAAGRR23 pKa = 11.84FVILRR28 pKa = 11.84ADD30 pKa = 3.78LEE32 pKa = 4.25NRR34 pKa = 11.84LPAIRR39 pKa = 11.84LLLSTITHH47 pKa = 5.67IRR49 pKa = 11.84VHH51 pKa = 5.72FTCSISVSTHH61 pKa = 5.31APEE64 pKa = 4.62TVNQSQILSQSARR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 3.6

Molecular weight: 8.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4152

0

4152

1354701

29

9197

326.3

36.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.008 ± 0.052

1.194 ± 0.019

5.111 ± 0.034

6.185 ± 0.045

3.873 ± 0.031

7.61 ± 0.053

1.823 ± 0.018

7.339 ± 0.034

5.679 ± 0.041

9.824 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.487 ± 0.02

4.331 ± 0.047

4.063 ± 0.034

3.895 ± 0.037

4.868 ± 0.035

5.68 ± 0.033

5.454 ± 0.042

7.128 ± 0.038

1.039 ± 0.013

3.409 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski