Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; Pseudomonas aeruginosa group; Pseudomonas aeruginosa

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5565 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G3XCY8|G3XCY8_PSEAE Probable outer membrane receptor for iron transport OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=PA4514 PE=1 SV=1
MM1 pKa = 7.75SDD3 pKa = 3.28SLQLILEE10 pKa = 4.72DD11 pKa = 3.55TDD13 pKa = 3.75GTQLEE18 pKa = 4.85TSCTRR23 pKa = 11.84FAVVWQGKK31 pKa = 7.29EE32 pKa = 3.65VWVQQAGNGQLLIGVDD48 pKa = 3.79VEE50 pKa = 5.66DD51 pKa = 5.76GDD53 pKa = 4.28TEE55 pKa = 4.34YY56 pKa = 11.28TNLLLRR62 pKa = 11.84PMATNLVSLQLEE74 pKa = 4.22MEE76 pKa = 5.07PIEE79 pKa = 4.7EE80 pKa = 4.61GDD82 pKa = 3.87DD83 pKa = 3.88HH84 pKa = 6.96EE85 pKa = 5.72HH86 pKa = 7.35GPDD89 pKa = 3.49CGCDD93 pKa = 3.11HH94 pKa = 6.63THH96 pKa = 6.66

Molecular weight: 10.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P37798|ACCC_PSEAE Biotin carboxylase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=accC PE=1 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.31RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29NGRR28 pKa = 11.84QVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5564

1

5565

1857520

10

5627

333.8

36.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.654 ± 0.042

1.003 ± 0.012

5.323 ± 0.02

6.088 ± 0.031

3.557 ± 0.021

8.447 ± 0.043

2.168 ± 0.015

4.174 ± 0.026

2.87 ± 0.03

12.455 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.06 ± 0.016

2.644 ± 0.025

5.078 ± 0.028

4.257 ± 0.025

7.618 ± 0.037

5.522 ± 0.024

4.169 ± 0.024

6.882 ± 0.028

1.488 ± 0.015

2.54 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski