Mycetocola lacteus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales;

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3070 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3L7ALP2|A0A3L7ALP2_9MICO Uncharacterized protein OS=Mycetocola lacteus OX=76637 GN=D9V34_11970 PE=4 SV=1
MM1 pKa = 7.24TGEE4 pKa = 4.2ALEE7 pKa = 4.58LANSTRR13 pKa = 11.84LGTVARR19 pKa = 11.84RR20 pKa = 11.84GLLAATLAVGLIIPALVPTAAFAAASATPGSVGQADD56 pKa = 3.88GTVTVSIAARR66 pKa = 11.84AAQLTLTPPSGVLIQRR82 pKa = 11.84PPTSFPCYY90 pKa = 9.47DD91 pKa = 3.39TGSGNTLIPASSTAFYY107 pKa = 9.97TGIYY111 pKa = 9.29CGYY114 pKa = 10.11SDD116 pKa = 3.8NTVGDD121 pKa = 3.78FTSYY125 pKa = 10.85AFAYY129 pKa = 9.18KK130 pKa = 10.22VSEE133 pKa = 4.02NAVPGSALGNFEE145 pKa = 4.09YY146 pKa = 10.42SVRR149 pKa = 11.84IPGSPNQTASAPLSVKK165 pKa = 9.56TLTAPTIATPTAGQLTNNPRR185 pKa = 11.84PVISGTADD193 pKa = 3.39NLPGTTLAVTITDD206 pKa = 3.59ADD208 pKa = 3.78TGVALAEE215 pKa = 4.16VPVVNGAWTYY225 pKa = 10.08TPVANRR231 pKa = 11.84PEE233 pKa = 4.21GTNNISVAASLNGVTTTATTRR254 pKa = 11.84SFVVDD259 pKa = 4.82TIPPAVPVLTSPVAGSATANTTPTVSGTGEE289 pKa = 3.72NGSRR293 pKa = 11.84IEE295 pKa = 3.91VRR297 pKa = 11.84NDD299 pKa = 2.76AGAVLCEE306 pKa = 4.01ATVVAGAWSCVVAPALTEE324 pKa = 4.38GSHH327 pKa = 5.78TLTPVALDD335 pKa = 3.66EE336 pKa = 5.24AGNAAPGTPVTFSVITTPPTAPVITAPTTGTITNNKK372 pKa = 7.95TPLITGTADD381 pKa = 2.58SGTIVEE387 pKa = 4.66IQNAANQLLCSGDD400 pKa = 3.55VPEE403 pKa = 5.99GEE405 pKa = 4.65FEE407 pKa = 4.58CVVAPALVDD416 pKa = 4.02GSHH419 pKa = 6.53TLTPISVDD427 pKa = 2.86AAGNRR432 pKa = 11.84TPGTPITIVVDD443 pKa = 4.21TVPPAAPVISSPATGTITKK462 pKa = 7.74NTRR465 pKa = 11.84PTFTGTGEE473 pKa = 3.88TGSRR477 pKa = 11.84VEE479 pKa = 3.49IHH481 pKa = 6.49GAQGQILCTAVVTAGAWSCQPATALEE507 pKa = 4.63DD508 pKa = 3.94GEE510 pKa = 4.67HH511 pKa = 6.97ALTAIAVDD519 pKa = 3.53TAGNRR524 pKa = 11.84TTGTSITITIDD535 pKa = 2.99TVAPTTPVITAPADD549 pKa = 3.49GTLTNNATPTITGTGEE565 pKa = 3.74NGSVIEE571 pKa = 4.38VRR573 pKa = 11.84GAEE576 pKa = 3.95NALLCTAVVAAGTWSCEE593 pKa = 3.53PATALGEE600 pKa = 4.41GSHH603 pKa = 6.25TLTPTARR610 pKa = 11.84DD611 pKa = 3.38AAGNTAPGTAVTITIDD627 pKa = 3.67TTPPAAPVITAPGDD641 pKa = 3.59GALLTTTTPTITGTGEE657 pKa = 3.69NGSTVEE663 pKa = 3.86VRR665 pKa = 11.84GGNNEE670 pKa = 4.06VLCTAVVTAGAWSCQISPALGQGDD694 pKa = 4.01HH695 pKa = 6.72SLTPVAMDD703 pKa = 3.73PAGNSTPGTAITVTVDD719 pKa = 3.29TVPPEE724 pKa = 4.22VPVITAPQNGTITNNATPTISGTGEE749 pKa = 3.69NGSTVEE755 pKa = 4.05VRR757 pKa = 11.84DD758 pKa = 3.51GAGTVLCTATVAAGIWSCTLDD779 pKa = 3.52EE780 pKa = 5.05PLTEE784 pKa = 4.97GDD786 pKa = 4.09HH787 pKa = 6.68PLLPVARR794 pKa = 11.84DD795 pKa = 3.32AAGNEE800 pKa = 4.08APGTAVTVTIDD811 pKa = 3.94LTPPAAPVVTAPLTGTATNVSTPVFTGTGEE841 pKa = 3.96NGSTVEE847 pKa = 4.11IRR849 pKa = 11.84AADD852 pKa = 3.77GTVLCSSLVEE862 pKa = 4.54DD863 pKa = 5.12GAWTCTIAPALDD875 pKa = 3.55EE876 pKa = 4.49GMHH879 pKa = 6.4ILSAVAVDD887 pKa = 3.43AAGNQTPGTIVALTVDD903 pKa = 4.28ITPPAVPAITSPVSGTITNDD923 pKa = 3.25TTPTLSGSGEE933 pKa = 4.08TGSTVEE939 pKa = 3.69IHH941 pKa = 5.85NAAGEE946 pKa = 4.4VLCSAVVTAGAWTCDD961 pKa = 3.51LTPALAEE968 pKa = 4.37GDD970 pKa = 3.91HH971 pKa = 6.29VLTPVAKK978 pKa = 10.31DD979 pKa = 3.15AAGNRR984 pKa = 11.84ATGLPITITVDD995 pKa = 3.52LTPPAVPVITSPVDD1009 pKa = 3.36GFVTNDD1015 pKa = 3.35TTPTLSGTGEE1025 pKa = 4.0TGSTVEE1031 pKa = 4.15IQNAAGDD1038 pKa = 4.21VLCSTVVTAGAWSCDD1053 pKa = 2.4ITTALTEE1060 pKa = 4.45GDD1062 pKa = 3.77HH1063 pKa = 6.67VLTPVAKK1070 pKa = 10.29DD1071 pKa = 3.02AAGNKK1076 pKa = 8.75TSGTAITITVDD1087 pKa = 3.08VTAPAVPVITSPVDD1101 pKa = 3.36GFVTNDD1107 pKa = 3.35TTPTLSGTGEE1117 pKa = 4.0TGSTVEE1123 pKa = 4.93IRR1125 pKa = 11.84NADD1128 pKa = 3.83DD1129 pKa = 3.91QVLCSAVVTAGAWTCDD1145 pKa = 3.56LAPALGDD1152 pKa = 3.7GNHH1155 pKa = 5.93VLTPVAKK1162 pKa = 10.29DD1163 pKa = 3.0AAGNKK1168 pKa = 8.65TPGTAITIEE1177 pKa = 4.68VITEE1181 pKa = 3.97LPGAPIVTSPTNGTITNDD1199 pKa = 3.09ATPTLTGTGEE1209 pKa = 3.96NGTTVEE1215 pKa = 4.15VRR1217 pKa = 11.84DD1218 pKa = 3.53AAGVVLCSAPVTDD1231 pKa = 4.77GTWSCVVAPALTDD1244 pKa = 3.73GDD1246 pKa = 4.36HH1247 pKa = 6.65EE1248 pKa = 4.46LVAVAVDD1255 pKa = 3.44AASNEE1260 pKa = 4.43TPGIPLTITVDD1271 pKa = 3.73TVPPAVPVITAPLDD1285 pKa = 3.55GTVTNVEE1292 pKa = 4.38SPTLSGSGEE1301 pKa = 4.11SGSTVEE1307 pKa = 4.25IQNAAGEE1314 pKa = 4.51VLCSVVVTAGAWSCEE1329 pKa = 3.87LAPALTEE1336 pKa = 4.3GDD1338 pKa = 3.73HH1339 pKa = 6.71VLTPVAKK1346 pKa = 10.23DD1347 pKa = 3.04AAGNEE1352 pKa = 4.39TPGVAITITVDD1363 pKa = 3.16VTPPAVPAITSPADD1377 pKa = 3.33GFVTNDD1383 pKa = 3.33TTPTLSGTGEE1393 pKa = 4.0TGSTVEE1399 pKa = 3.69IHH1401 pKa = 5.85NAAGEE1406 pKa = 4.4VLCSAVVTAGAWTCDD1421 pKa = 3.17LATTLTEE1428 pKa = 4.34GDD1430 pKa = 3.76HH1431 pKa = 6.55VLTPVAKK1438 pKa = 10.29DD1439 pKa = 3.0AAGNKK1444 pKa = 8.59TPGTPITITVDD1455 pKa = 3.18VTPPTVPVITSPANGLVTNDD1475 pKa = 3.53TTPTLSGTGEE1485 pKa = 4.05NGSTVTVKK1493 pKa = 10.51NAADD1497 pKa = 3.76EE1498 pKa = 4.78VVCSAVVSNGAWSCTLDD1515 pKa = 3.38SALTEE1520 pKa = 4.44GDD1522 pKa = 3.66HH1523 pKa = 6.73VLTPVATDD1531 pKa = 3.27AAGNKK1536 pKa = 8.25ATGTAITITVDD1547 pKa = 3.2TTAPAAPVIATPSANIVTNNTTPTLAGTGEE1577 pKa = 4.17TGSTVEE1583 pKa = 4.06IRR1585 pKa = 11.84NAAGVVLCSAEE1596 pKa = 4.24VVDD1599 pKa = 5.15GAWSCVVAPALTDD1612 pKa = 3.4GEE1614 pKa = 4.63YY1615 pKa = 10.4PLTAVAVDD1623 pKa = 3.27AAGNEE1628 pKa = 4.51TEE1630 pKa = 4.54GSSVTITVKK1639 pKa = 8.25TTLPAAPVITSPTNGSVGNNPAPTLSGTGEE1669 pKa = 4.01NGTTVEE1675 pKa = 3.89VRR1677 pKa = 11.84GPGGEE1682 pKa = 4.31VLCSAVVTNGTWSCEE1697 pKa = 3.86PSPEE1701 pKa = 4.1LTEE1704 pKa = 4.69GDD1706 pKa = 3.54HH1707 pKa = 6.35TLTPVAVDD1715 pKa = 3.0IAGNEE1720 pKa = 4.36TPGNPITLTVDD1731 pKa = 3.68TTPPTPTTDD1740 pKa = 3.35VVCSVNQLGEE1750 pKa = 4.38VTCEE1754 pKa = 3.65GTAAEE1759 pKa = 4.85GDD1761 pKa = 4.18TIVVTDD1767 pKa = 3.95GDD1769 pKa = 3.92GKK1771 pKa = 10.09EE1772 pKa = 3.84ICTVEE1777 pKa = 4.55VPAGGHH1783 pKa = 4.33WTCTGGPVTSFPVTVEE1799 pKa = 4.18TVDD1802 pKa = 3.39PAGNSGGVHH1811 pKa = 6.24TLPTPPIIVSPNTGSVLGEE1830 pKa = 4.07STPTLTGTGAAGDD1843 pKa = 4.03TVEE1846 pKa = 5.48LLDD1849 pKa = 4.15AEE1851 pKa = 4.64GSVVCTTTVEE1861 pKa = 4.68ADD1863 pKa = 3.92GTWSCTLPAALADD1876 pKa = 4.03GTHH1879 pKa = 6.52TLTPVATDD1887 pKa = 3.27ADD1889 pKa = 4.44GNTFTGIAISLNIDD1903 pKa = 3.46TVAPSPPTGVEE1914 pKa = 3.97CSANADD1920 pKa = 3.66GTVTCSGTAEE1930 pKa = 4.17PDD1932 pKa = 3.44STVEE1936 pKa = 3.76ITDD1939 pKa = 3.5PEE1941 pKa = 4.53GNLICEE1947 pKa = 4.5VTVGADD1953 pKa = 3.76GTWTCTSEE1961 pKa = 4.2GAVDD1965 pKa = 3.96AGEE1968 pKa = 4.04LTITVIDD1975 pKa = 3.81PAGNRR1980 pKa = 11.84SPSISIGVTPLPGTPGPGTTDD2001 pKa = 3.24PGAPGPGTPGPGTPGPGTPGPGTPGPGAGGGSLVTTGADD2040 pKa = 3.24AVGMGALALAAMLLLGSGVFLRR2062 pKa = 11.84SRR2064 pKa = 11.84RR2065 pKa = 11.84TSGTTRR2071 pKa = 11.84GEE2073 pKa = 4.19DD2074 pKa = 3.05

Molecular weight: 203.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3L7AHK3|A0A3L7AHK3_9MICO Succinate dehydrogenase flavoprotein subunit OS=Mycetocola lacteus OX=76637 GN=D9V34_15050 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.31LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3070

0

3070

1024074

29

2610

333.6

35.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.047 ± 0.071

0.465 ± 0.01

5.503 ± 0.035

5.609 ± 0.047

3.22 ± 0.028

8.972 ± 0.042

1.885 ± 0.02

5.228 ± 0.032

2.21 ± 0.042

10.351 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.67 ± 0.018

2.493 ± 0.028

5.556 ± 0.039

2.775 ± 0.024

6.78 ± 0.05

5.92 ± 0.036

6.696 ± 0.054

8.263 ± 0.041

1.394 ± 0.018

1.964 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski