Streptococcus phage Javan116

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ASZ0|A0A4D6ASZ0_9CAUD Uncharacterized protein OS=Streptococcus phage Javan116 OX=2547985 GN=Javan116_0038 PE=4 SV=1
MM1 pKa = 7.56EE2 pKa = 4.46LHH4 pKa = 6.67EE5 pKa = 4.62FASLLTQTDD14 pKa = 4.14LLVQYY19 pKa = 10.84HH20 pKa = 5.63SFKK23 pKa = 11.06KK24 pKa = 10.47GDD26 pKa = 3.99APNPPYY32 pKa = 10.2IVYY35 pKa = 8.61TAIKK39 pKa = 8.86PEE41 pKa = 4.11TVRR44 pKa = 11.84GDD46 pKa = 3.23NEE48 pKa = 3.96RR49 pKa = 11.84YY50 pKa = 9.03YY51 pKa = 10.86QIDD54 pKa = 3.7TVLVEE59 pKa = 4.88LVTDD63 pKa = 5.08LKK65 pKa = 11.4DD66 pKa = 3.44LDD68 pKa = 4.07LEE70 pKa = 5.05RR71 pKa = 11.84ILEE74 pKa = 4.2SLFDD78 pKa = 4.16EE79 pKa = 4.55NKK81 pKa = 10.3LFYY84 pKa = 10.51EE85 pKa = 4.73IEE87 pKa = 4.48DD88 pKa = 3.61EE89 pKa = 4.39SHH91 pKa = 6.91IEE93 pKa = 4.01TEE95 pKa = 4.06EE96 pKa = 4.41LFVKK100 pKa = 10.51SYY102 pKa = 11.09LVYY105 pKa = 10.66LYY107 pKa = 11.15

Molecular weight: 12.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ATT0|A0A4D6ATT0_9CAUD Major tail protein OS=Streptococcus phage Javan116 OX=2547985 GN=Javan116_0029 PE=4 SV=1
MM1 pKa = 7.24NLVEE5 pKa = 5.19PIRR8 pKa = 11.84DD9 pKa = 3.49KK10 pKa = 11.73DD11 pKa = 4.28DD12 pKa = 3.22IQAMKK17 pKa = 10.57DD18 pKa = 3.68YY19 pKa = 10.86LKK21 pKa = 10.6EE22 pKa = 4.18WNDD25 pKa = 3.28RR26 pKa = 11.84NYY28 pKa = 9.91MLFMVGINSGFRR40 pKa = 11.84ISDD43 pKa = 3.4IVKK46 pKa = 10.53LKK48 pKa = 11.06VKK50 pKa = 10.23DD51 pKa = 3.52VQGWHH56 pKa = 6.18FKK58 pKa = 10.39IKK60 pKa = 8.24EE61 pKa = 3.98QKK63 pKa = 7.29TGKK66 pKa = 9.82IIRR69 pKa = 11.84RR70 pKa = 11.84KK71 pKa = 8.61MPRR74 pKa = 11.84PLKK77 pKa = 10.78NEE79 pKa = 3.83LARR82 pKa = 11.84YY83 pKa = 7.54TEE85 pKa = 4.57GKK87 pKa = 8.85PLHH90 pKa = 6.87HH91 pKa = 6.99YY92 pKa = 10.04LFQSRR97 pKa = 11.84KK98 pKa = 9.79GKK100 pKa = 8.92NQPITRR106 pKa = 11.84QAAYY110 pKa = 10.55LIIKK114 pKa = 7.56TAAEE118 pKa = 3.88DD119 pKa = 3.67LGIEE123 pKa = 4.15NVGTHH128 pKa = 5.32TMRR131 pKa = 11.84KK132 pKa = 6.66TFGYY136 pKa = 9.97HH137 pKa = 5.37YY138 pKa = 9.25YY139 pKa = 10.67EE140 pKa = 4.78KK141 pKa = 10.59YY142 pKa = 10.59KK143 pKa = 10.96DD144 pKa = 3.67VASLMEE150 pKa = 4.46MFNHH154 pKa = 7.2ASPAITLRR162 pKa = 11.84YY163 pKa = 8.04IGKK166 pKa = 8.9RR167 pKa = 11.84QEE169 pKa = 3.99QLDD172 pKa = 3.64LSMAKK177 pKa = 10.71FSLL180 pKa = 3.86

Molecular weight: 21.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

8202

37

930

186.4

21.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.815 ± 0.566

0.61 ± 0.113

6.352 ± 0.405

7.913 ± 0.445

4.084 ± 0.28

5.95 ± 0.428

1.378 ± 0.161

7.218 ± 0.234

8.01 ± 0.404

8.376 ± 0.316

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.865 ± 0.248

5.584 ± 0.331

2.877 ± 0.238

4.072 ± 0.321

4.572 ± 0.332

6.437 ± 0.393

5.304 ± 0.27

6.376 ± 0.269

1.256 ± 0.134

3.95 ± 0.428
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski