Pseudomonas phage MR14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3TDR6|A0A6M3TDR6_9CAUD Uncharacterized protein OS=Pseudomonas phage MR14 OX=2711178 GN=Psm1vBMR14_gp27 PE=4 SV=1
MM1 pKa = 7.45ACEE4 pKa = 3.99TSAFLGRR11 pKa = 11.84DD12 pKa = 3.18TSLEE16 pKa = 3.93FAIGCGDD23 pKa = 3.75QLPAEE28 pKa = 4.84ADD30 pKa = 3.34WKK32 pKa = 11.07FIGALRR38 pKa = 11.84SNSLSGEE45 pKa = 4.07YY46 pKa = 9.45DD47 pKa = 3.66TVDD50 pKa = 3.2TTADD54 pKa = 3.58DD55 pKa = 3.64SAGGIRR61 pKa = 11.84EE62 pKa = 3.82MLATFKK68 pKa = 10.88SITISTDD75 pKa = 2.68GVARR79 pKa = 11.84RR80 pKa = 11.84ADD82 pKa = 3.5TATVNQTALLLHH94 pKa = 6.57YY95 pKa = 8.63YY96 pKa = 10.55NPVATGGQPVVWLRR110 pKa = 11.84RR111 pKa = 11.84TDD113 pKa = 3.44PRR115 pKa = 11.84ITLVGYY121 pKa = 9.96FLMSTYY127 pKa = 10.85DD128 pKa = 3.56LEE130 pKa = 4.46SPYY133 pKa = 11.36DD134 pKa = 3.75EE135 pKa = 4.84VSTFSIEE142 pKa = 4.28ASTTANSVIATGVSITATPVVV163 pKa = 3.62

Molecular weight: 17.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3TDY3|A0A6M3TDY3_9CAUD Putative transcriptional regulator OS=Pseudomonas phage MR14 OX=2711178 GN=Psm1vBMR14_gp42c PE=4 SV=1
MM1 pKa = 7.53TGMPFWDD8 pKa = 4.02AYY10 pKa = 10.07KK11 pKa = 10.14PHH13 pKa = 7.46LYY15 pKa = 9.72NANRR19 pKa = 11.84RR20 pKa = 11.84VRR22 pKa = 11.84QRR24 pKa = 11.84QQRR27 pKa = 11.84TEE29 pKa = 3.9NPVSQRR35 pKa = 11.84YY36 pKa = 9.03AIRR39 pKa = 11.84ARR41 pKa = 11.84WGMEE45 pKa = 3.4WYY47 pKa = 9.73EE48 pKa = 4.14LLALLHH54 pKa = 6.11AQGLNRR60 pKa = 11.84QQCAQAMGYY69 pKa = 8.91SCWGSVKK76 pKa = 10.78AMLTRR81 pKa = 11.84HH82 pKa = 6.53PGNDD86 pKa = 3.1PFTPRR91 pKa = 11.84VLKK94 pKa = 10.11PKK96 pKa = 9.98RR97 pKa = 11.84QAPRR101 pKa = 11.84PRR103 pKa = 11.84FPRR106 pKa = 11.84RR107 pKa = 11.84HH108 pKa = 5.48AVEE111 pKa = 3.94QKK113 pKa = 9.82FGKK116 pKa = 10.21DD117 pKa = 3.13FWSVIRR123 pKa = 11.84DD124 pKa = 3.77FAEE127 pKa = 3.93KK128 pKa = 10.39DD129 pKa = 3.25YY130 pKa = 11.41TRR132 pKa = 11.84FDD134 pKa = 3.24TARR137 pKa = 11.84AIGCEE142 pKa = 3.67EE143 pKa = 4.12STFLRR148 pKa = 11.84ILADD152 pKa = 3.8NPKK155 pKa = 9.57HH156 pKa = 7.28DD157 pKa = 4.7PFEE160 pKa = 4.17SHH162 pKa = 6.38NVVGNYY168 pKa = 7.34TRR170 pKa = 11.84LTGEE174 pKa = 3.97SFEE177 pKa = 4.18AALRR181 pKa = 11.84RR182 pKa = 11.84MAAAGYY188 pKa = 8.86SRR190 pKa = 11.84RR191 pKa = 11.84QASIEE196 pKa = 3.54IGYY199 pKa = 9.22YY200 pKa = 9.93PSGLSLQRR208 pKa = 11.84AMDD211 pKa = 3.21IRR213 pKa = 11.84GIDD216 pKa = 3.46VTFKK220 pKa = 10.82KK221 pKa = 10.59AEE223 pKa = 4.08KK224 pKa = 9.33PKK226 pKa = 10.47KK227 pKa = 8.33PKK229 pKa = 10.12KK230 pKa = 9.44PRR232 pKa = 11.84EE233 pKa = 3.75PSISGRR239 pKa = 11.84YY240 pKa = 7.83GPKK243 pKa = 9.98SAIHH247 pKa = 6.61PWRR250 pKa = 11.84ISS252 pKa = 3.06

Molecular weight: 29.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

14591

40

1079

231.6

25.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.857 ± 0.486

1.288 ± 0.178

5.49 ± 0.227

5.407 ± 0.323

3.098 ± 0.16

8.128 ± 0.259

1.816 ± 0.237

4.215 ± 0.17

4.14 ± 0.343

8.45 ± 0.204

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.316 ± 0.151

3.591 ± 0.226

5.14 ± 0.393

3.941 ± 0.264

6.189 ± 0.282

6.408 ± 0.284

6.895 ± 0.282

6.881 ± 0.234

1.864 ± 0.167

2.885 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski