Amino acid dipepetide frequency for Sulfobacillus acidophilus (strain ATCC 700253 / DSM 10332 / NAL)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.727AlaAla: 10.727 ± 0.119	0.603AlaCys: 0.603 ± 0.03	5.05AlaAsp: 5.05 ± 0.084	6.049AlaGlu: 6.049 ± 0.09	3.534AlaPhe: 3.534 ± 0.074	8.589AlaGly: 8.589 ± 0.098	2.543AlaHis: 2.543 ± 0.056	5.875AlaIle: 5.875 ± 0.08	2.454AlaLys: 2.454 ± 0.058	12.556AlaLeu: 12.556 ± 0.12	2.579AlaMet: 2.579 ± 0.055	2.259AlaAsn: 2.259 ± 0.046	4.606AlaPro: 4.606 ± 0.067	4.082AlaGln: 4.082 ± 0.079	6.95AlaArg: 6.95 ± 0.098	4.884AlaSer: 4.884 ± 0.076	5.242AlaThr: 5.242 ± 0.079	9.389AlaVal: 9.389 ± 0.101	2.346AlaTrp: 2.346 ± 0.057	2.702AlaTyr: 2.702 ± 0.06	0.0AlaXaa: 0.0 ± 0.0
Cys	0.387CysAla: 0.387 ± 0.024	0.061CysCys: 0.061 ± 0.008	0.276CysAsp: 0.276 ± 0.017	0.237CysGlu: 0.237 ± 0.017	0.141CysPhe: 0.141 ± 0.012	0.669CysGly: 0.669 ± 0.031	0.236CysHis: 0.236 ± 0.015	0.194CysIle: 0.194 ± 0.013	0.086CysLys: 0.086 ± 0.01	0.483CysLeu: 0.483 ± 0.024	0.088CysMet: 0.088 ± 0.009	0.119CysAsn: 0.119 ± 0.013	0.51CysPro: 0.51 ± 0.03	0.33CysGln: 0.33 ± 0.018	0.495CysArg: 0.495 ± 0.027	0.284CysSer: 0.284 ± 0.019	0.333CysThr: 0.333 ± 0.023	0.32CysVal: 0.32 ± 0.022	0.102CysTrp: 0.102 ± 0.01	0.174CysTyr: 0.174 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	4.91AspAla: 4.91 ± 0.077	0.247AspCys: 0.247 ± 0.017	2.444AspAsp: 2.444 ± 0.053	2.862AspGlu: 2.862 ± 0.058	1.608AspPhe: 1.608 ± 0.039	3.774AspGly: 3.774 ± 0.068	1.466AspHis: 1.466 ± 0.047	2.635AspIle: 2.635 ± 0.059	0.992AspLys: 0.992 ± 0.034	4.963AspLeu: 4.963 ± 0.081	1.048AspMet: 1.048 ± 0.031	1.044AspAsn: 1.044 ± 0.035	3.572AspPro: 3.572 ± 0.062	2.182AspGln: 2.182 ± 0.046	4.17AspArg: 4.17 ± 0.063	1.839AspSer: 1.839 ± 0.041	2.523AspThr: 2.523 ± 0.054	4.013AspVal: 4.013 ± 0.073	1.289AspTrp: 1.289 ± 0.043	1.33AspTyr: 1.33 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	6.763GluAla: 6.763 ± 0.108	0.254GluCys: 0.254 ± 0.016	2.515GluAsp: 2.515 ± 0.05	3.357GluGlu: 3.357 ± 0.07	1.559GluPhe: 1.559 ± 0.045	3.596GluGly: 3.596 ± 0.065	1.188GluHis: 1.188 ± 0.033	2.931GluIle: 2.931 ± 0.057	1.57GluLys: 1.57 ± 0.044	4.995GluLeu: 4.995 ± 0.082	1.334GluMet: 1.334 ± 0.034	1.259GluAsn: 1.259 ± 0.037	2.792GluPro: 2.792 ± 0.065	2.03GluGln: 2.03 ± 0.049	4.591GluArg: 4.591 ± 0.081	2.915GluSer: 2.915 ± 0.053	3.284GluThr: 3.284 ± 0.059	4.016GluVal: 4.016 ± 0.065	1.347GluTrp: 1.347 ± 0.038	1.172GluTyr: 1.172 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	2.639PheAla: 2.639 ± 0.055	0.253PheCys: 0.253 ± 0.017	1.745PheAsp: 1.745 ± 0.044	1.555PheGlu: 1.555 ± 0.045	1.364PhePhe: 1.364 ± 0.045	3.181PheGly: 3.181 ± 0.064	1.085PheHis: 1.085 ± 0.032	1.615PheIle: 1.615 ± 0.047	0.611PheLys: 0.611 ± 0.027	3.83PheLeu: 3.83 ± 0.07	0.738PheMet: 0.738 ± 0.028	0.893PheAsn: 0.893 ± 0.032	1.822PhePro: 1.822 ± 0.048	1.449PheGln: 1.449 ± 0.038	2.502PheArg: 2.502 ± 0.051	2.145PheSer: 2.145 ± 0.057	1.727PheThr: 1.727 ± 0.042	2.547PheVal: 2.547 ± 0.057	0.901PheTrp: 0.901 ± 0.032	1.115PheTyr: 1.115 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	6.953GlyAla: 6.953 ± 0.078	0.585GlyCys: 0.585 ± 0.029	3.499GlyAsp: 3.499 ± 0.068	3.759GlyGlu: 3.759 ± 0.059	3.489GlyPhe: 3.489 ± 0.063	6.32GlyGly: 6.32 ± 0.106	2.301GlyHis: 2.301 ± 0.052	5.227GlyIle: 5.227 ± 0.085	2.051GlyLys: 2.051 ± 0.049	9.597GlyLeu: 9.597 ± 0.124	2.18GlyMet: 2.18 ± 0.054	1.792GlyAsn: 1.792 ± 0.047	4.228GlyPro: 4.228 ± 0.067	3.832GlyGln: 3.832 ± 0.069	5.573GlyArg: 5.573 ± 0.089	4.392GlySer: 4.392 ± 0.069	4.611GlyThr: 4.611 ± 0.086	6.617GlyVal: 6.617 ± 0.081	2.12GlyTrp: 2.12 ± 0.046	2.771GlyTyr: 2.771 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	2.274HisAla: 2.274 ± 0.048	0.169HisCys: 0.169 ± 0.015	1.234HisAsp: 1.234 ± 0.035	1.232HisGlu: 1.232 ± 0.037	0.86HisPhe: 0.86 ± 0.03	2.16HisGly: 2.16 ± 0.044	1.03HisHis: 1.03 ± 0.036	1.193HisIle: 1.193 ± 0.036	0.42HisLys: 0.42 ± 0.019	2.845HisLeu: 2.845 ± 0.049	0.511HisMet: 0.511 ± 0.021	0.557HisAsn: 0.557 ± 0.022	2.247HisPro: 2.247 ± 0.054	1.437HisGln: 1.437 ± 0.038	2.122HisArg: 2.122 ± 0.047	1.079HisSer: 1.079 ± 0.035	1.274HisThr: 1.274 ± 0.035	1.999HisVal: 1.999 ± 0.039	0.82HisTrp: 0.82 ± 0.027	0.824HisTyr: 0.824 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.261IleAla: 5.261 ± 0.084	0.261IleCys: 0.261 ± 0.015	2.765IleAsp: 2.765 ± 0.046	2.791IleGlu: 2.791 ± 0.056	1.758IlePhe: 1.758 ± 0.05	4.634IleGly: 4.634 ± 0.08	1.394IleHis: 1.394 ± 0.036	2.389IleIle: 2.389 ± 0.053	1.136IleLys: 1.136 ± 0.039	5.416IleLeu: 5.416 ± 0.087	1.028IleMet: 1.028 ± 0.031	1.392IleAsn: 1.392 ± 0.041	3.353IlePro: 3.353 ± 0.057	2.327IleGln: 2.327 ± 0.045	4.338IleArg: 4.338 ± 0.057	2.587IleSer: 2.587 ± 0.054	2.983IleThr: 2.983 ± 0.054	4.088IleVal: 4.088 ± 0.065	1.057IleTrp: 1.057 ± 0.036	1.34IleTyr: 1.34 ± 0.042	0.0IleXaa: 0.0 ± 0.0
Lys	2.809LysAla: 2.809 ± 0.061	0.089LysCys: 0.089 ± 0.01	1.245LysAsp: 1.245 ± 0.036	1.428LysGlu: 1.428 ± 0.042	0.581LysPhe: 0.581 ± 0.023	1.687LysGly: 1.687 ± 0.05	0.442LysHis: 0.442 ± 0.021	1.368LysIle: 1.368 ± 0.041	0.924LysLys: 0.924 ± 0.037	1.991LysLeu: 1.991 ± 0.048	0.611LysMet: 0.611 ± 0.023	0.754LysAsn: 0.754 ± 0.027	1.626LysPro: 1.626 ± 0.044	0.707LysGln: 0.707 ± 0.027	1.779LysArg: 1.779 ± 0.04	1.426LysSer: 1.426 ± 0.041	1.882LysThr: 1.882 ± 0.045	1.72LysVal: 1.72 ± 0.044	0.454LysTrp: 0.454 ± 0.02	0.56LysTyr: 0.56 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	13.575LeuAla: 13.575 ± 0.131	0.48LeuCys: 0.48 ± 0.022	5.234LeuAsp: 5.234 ± 0.078	5.55LeuGlu: 5.55 ± 0.093	3.453LeuPhe: 3.453 ± 0.069	9.343LeuGly: 9.343 ± 0.117	2.153LeuHis: 2.153 ± 0.041	5.311LeuIle: 5.311 ± 0.083	3.289LeuLys: 3.289 ± 0.06	10.637LeuLeu: 10.637 ± 0.138	2.576LeuMet: 2.576 ± 0.053	2.751LeuAsn: 2.751 ± 0.052	5.949LeuPro: 5.949 ± 0.077	3.277LeuGln: 3.277 ± 0.059	6.287LeuArg: 6.287 ± 0.086	6.46LeuSer: 6.46 ± 0.082	7.497LeuThr: 7.497 ± 0.084	8.731LeuVal: 8.731 ± 0.115	2.579LeuTrp: 2.579 ± 0.064	2.668LeuTyr: 2.668 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	3.259MetAla: 3.259 ± 0.056	0.092MetCys: 0.092 ± 0.01	1.1MetAsp: 1.1 ± 0.039	1.171MetGlu: 1.171 ± 0.035	0.594MetPhe: 0.594 ± 0.024	1.988MetGly: 1.988 ± 0.044	0.433MetHis: 0.433 ± 0.023	1.244MetIle: 1.244 ± 0.037	0.757MetLys: 0.757 ± 0.025	1.952MetLeu: 1.952 ± 0.048	0.652MetMet: 0.652 ± 0.024	0.757MetAsn: 0.757 ± 0.025	1.407MetPro: 1.407 ± 0.04	0.744MetGln: 0.744 ± 0.027	1.538MetArg: 1.538 ± 0.034	1.237MetSer: 1.237 ± 0.04	1.899MetThr: 1.899 ± 0.045	2.066MetVal: 2.066 ± 0.047	0.29MetTrp: 0.29 ± 0.016	0.324MetTyr: 0.324 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.352AsnAla: 2.352 ± 0.052	0.129AsnCys: 0.129 ± 0.01	0.922AsnAsp: 0.922 ± 0.033	0.919AsnGlu: 0.919 ± 0.034	0.733AsnPhe: 0.733 ± 0.029	2.04AsnGly: 2.04 ± 0.052	0.686AsnHis: 0.686 ± 0.028	1.131AsnIle: 1.131 ± 0.038	0.436AsnLys: 0.436 ± 0.026	2.485AsnLeu: 2.485 ± 0.051	0.475AsnMet: 0.475 ± 0.02	0.584AsnAsn: 0.584 ± 0.028	2.465AsnPro: 2.465 ± 0.052	1.291AsnGln: 1.291 ± 0.04	2.032AsnArg: 2.032 ± 0.046	0.96AsnSer: 0.96 ± 0.034	1.267AsnThr: 1.267 ± 0.043	1.679AsnVal: 1.679 ± 0.044	0.505AsnTrp: 0.505 ± 0.023	0.547AsnTyr: 0.547 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	5.792ProAla: 5.792 ± 0.074	0.234ProCys: 0.234 ± 0.017	3.94ProAsp: 3.94 ± 0.074	3.962ProGlu: 3.962 ± 0.076	2.005ProPhe: 2.005 ± 0.039	5.129ProGly: 5.129 ± 0.079	1.579ProHis: 1.579 ± 0.039	2.535ProIle: 2.535 ± 0.051	1.457ProLys: 1.457 ± 0.035	6.096ProLeu: 6.096 ± 0.08	1.282ProMet: 1.282 ± 0.039	1.421ProAsn: 1.421 ± 0.036	3.437ProPro: 3.437 ± 0.075	1.934ProGln: 1.934 ± 0.044	3.52ProArg: 3.52 ± 0.07	3.246ProSer: 3.246 ± 0.066	3.34ProThr: 3.34 ± 0.06	5.493ProVal: 5.493 ± 0.084	1.397ProTrp: 1.397 ± 0.045	1.706ProTyr: 1.706 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	5.062GlnAla: 5.062 ± 0.078	0.222GlnCys: 0.222 ± 0.016	1.856GlnAsp: 1.856 ± 0.043	2.354GlnGlu: 2.354 ± 0.047	1.277GlnPhe: 1.277 ± 0.037	3.133GlnGly: 3.133 ± 0.057	1.011GlnHis: 1.011 ± 0.031	2.069GlnIle: 2.069 ± 0.044	1.403GlnLys: 1.403 ± 0.04	4.028GlnLeu: 4.028 ± 0.058	1.019GlnMet: 1.019 ± 0.03	1.061GlnAsn: 1.061 ± 0.035	2.027GlnPro: 2.027 ± 0.05	1.895GlnGln: 1.895 ± 0.051	2.684GlnArg: 2.684 ± 0.056	2.192GlnSer: 2.192 ± 0.053	2.289GlnThr: 2.289 ± 0.051	3.342GlnVal: 3.342 ± 0.064	1.184GlnTrp: 1.184 ± 0.034	1.193GlnTyr: 1.193 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	5.938ArgAla: 5.938 ± 0.072	0.426ArgCys: 0.426 ± 0.023	3.438ArgAsp: 3.438 ± 0.068	4.17ArgGlu: 4.17 ± 0.07	2.538ArgPhe: 2.538 ± 0.045	4.485ArgGly: 4.485 ± 0.074	2.416ArgHis: 2.416 ± 0.048	3.984ArgIle: 3.984 ± 0.071	1.49ArgLys: 1.49 ± 0.039	8.973ArgLeu: 8.973 ± 0.119	1.804ArgMet: 1.804 ± 0.04	1.275ArgAsn: 1.275 ± 0.035	4.15ArgPro: 4.15 ± 0.073	4.551ArgGln: 4.551 ± 0.074	6.261ArgArg: 6.261 ± 0.101	2.998ArgSer: 2.998 ± 0.063	3.434ArgThr: 3.434 ± 0.057	5.771ArgVal: 5.771 ± 0.087	2.005ArgTrp: 2.005 ± 0.043	2.418ArgTyr: 2.418 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	4.9SerAla: 4.9 ± 0.064	0.317SerCys: 0.317 ± 0.022	2.276SerAsp: 2.276 ± 0.048	2.361SerGlu: 2.361 ± 0.046	1.689SerPhe: 1.689 ± 0.042	4.775SerGly: 4.775 ± 0.074	1.425SerHis: 1.425 ± 0.037	2.244SerIle: 2.244 ± 0.051	0.919SerLys: 0.919 ± 0.035	5.955SerLeu: 5.955 ± 0.089	1.278SerMet: 1.278 ± 0.033	1.063SerAsn: 1.063 ± 0.036	3.516SerPro: 3.516 ± 0.059	2.159SerGln: 2.159 ± 0.052	4.089SerArg: 4.089 ± 0.067	2.888SerSer: 2.888 ± 0.066	2.55SerThr: 2.55 ± 0.048	4.175SerVal: 4.175 ± 0.07	1.157SerTrp: 1.157 ± 0.038	1.329SerTyr: 1.329 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	5.955ThrAla: 5.955 ± 0.072	0.317ThrCys: 0.317 ± 0.019	2.643ThrAsp: 2.643 ± 0.05	2.881ThrGlu: 2.881 ± 0.053	1.938ThrPhe: 1.938 ± 0.05	5.467ThrGly: 5.467 ± 0.068	1.367ThrHis: 1.367 ± 0.038	2.96ThrIle: 2.96 ± 0.058	1.081ThrLys: 1.081 ± 0.03	6.343ThrLeu: 6.343 ± 0.09	1.177ThrMet: 1.177 ± 0.035	1.269ThrAsn: 1.269 ± 0.038	4.018ThrPro: 4.018 ± 0.073	1.788ThrGln: 1.788 ± 0.045	3.88ThrArg: 3.88 ± 0.071	2.496ThrSer: 2.496 ± 0.047	3.212ThrThr: 3.212 ± 0.075	6.036ThrVal: 6.036 ± 0.074	1.132ThrTrp: 1.132 ± 0.036	1.482ThrTyr: 1.482 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	8.528ValAla: 8.528 ± 0.084	0.42ValCys: 0.42 ± 0.021	4.205ValAsp: 4.205 ± 0.063	4.48ValGlu: 4.48 ± 0.074	2.841ValPhe: 2.841 ± 0.058	6.641ValGly: 6.641 ± 0.078	1.853ValHis: 1.853 ± 0.044	4.871ValIle: 4.871 ± 0.071	2.178ValLys: 2.178 ± 0.055	8.892ValLeu: 8.892 ± 0.1	2.121ValMet: 2.121 ± 0.046	2.227ValAsn: 2.227 ± 0.054	4.754ValPro: 4.754 ± 0.067	2.56ValGln: 2.56 ± 0.049	5.357ValArg: 5.357 ± 0.083	4.785ValSer: 4.785 ± 0.078	5.534ValThr: 5.534 ± 0.081	7.543ValVal: 7.543 ± 0.093	1.679ValTrp: 1.679 ± 0.041	1.99ValTyr: 1.99 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	2.461TrpAla: 2.461 ± 0.054	0.145TrpCys: 0.145 ± 0.012	1.183TrpAsp: 1.183 ± 0.038	1.127TrpGlu: 1.127 ± 0.035	0.715TrpPhe: 0.715 ± 0.028	1.82TrpGly: 1.82 ± 0.041	0.711TrpHis: 0.711 ± 0.028	1.425TrpIle: 1.425 ± 0.042	0.42TrpLys: 0.42 ± 0.021	2.666TrpLeu: 2.666 ± 0.056	0.613TrpMet: 0.613 ± 0.026	0.569TrpAsn: 0.569 ± 0.025	1.356TrpPro: 1.356 ± 0.043	1.195TrpGln: 1.195 ± 0.032	1.673TrpArg: 1.673 ± 0.043	1.15TrpSer: 1.15 ± 0.032	1.309TrpThr: 1.309 ± 0.036	2.008TrpVal: 2.008 ± 0.051	0.586TrpTrp: 0.586 ± 0.03	0.493TrpTyr: 0.493 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.476TyrAla: 2.476 ± 0.047	0.222TyrCys: 0.222 ± 0.015	1.339TyrAsp: 1.339 ± 0.038	1.22TyrGlu: 1.22 ± 0.035	1.043TyrPhe: 1.043 ± 0.034	2.466TyrGly: 2.466 ± 0.047	0.964TyrHis: 0.964 ± 0.03	1.1TyrIle: 1.1 ± 0.035	0.41TyrLys: 0.41 ± 0.022	3.096TyrLeu: 3.096 ± 0.057	0.388TyrMet: 0.388 ± 0.02	0.593TyrAsn: 0.593 ± 0.03	1.671TyrPro: 1.671 ± 0.048	1.533TyrGln: 1.533 ± 0.038	2.685TyrArg: 2.685 ± 0.051	1.104TyrSer: 1.104 ± 0.036	1.252TyrThr: 1.252 ± 0.035	1.903TyrVal: 1.903 ± 0.039	0.675TyrTrp: 0.675 ± 0.024	0.787TyrTyr: 0.787 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3444 proteins (1017646 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---