Aliikangiella sp. M105

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Pleioneaceae; Aliikangiella

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5286 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A545TWD7|A0A545TWD7_9GAMM Uncharacterized protein OS=Aliikangiella sp. M105 OX=2592383 GN=FLL46_25655 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.28KK3 pKa = 10.37VLGALAIASVTLAPAAYY20 pKa = 9.97ADD22 pKa = 3.29IAGFEE27 pKa = 4.08VGAYY31 pKa = 8.07QWTPDD36 pKa = 3.45YY37 pKa = 10.57TGTISSDD44 pKa = 3.25SGSVLGSSIDD54 pKa = 3.41IQNDD58 pKa = 3.13LGFDD62 pKa = 3.92DD63 pKa = 6.11DD64 pKa = 4.08SHH66 pKa = 7.45NVIWASLEE74 pKa = 4.03HH75 pKa = 6.57PVPFLPNIKK84 pKa = 9.87VVSSDD89 pKa = 3.51LDD91 pKa = 3.58NAASSTLTRR100 pKa = 11.84EE101 pKa = 3.81IVFGGEE107 pKa = 4.04TYY109 pKa = 10.8SVNEE113 pKa = 4.07AVSTTFDD120 pKa = 3.1TSNTEE125 pKa = 3.44FTFYY129 pKa = 11.51YY130 pKa = 10.26EE131 pKa = 5.94ILDD134 pKa = 3.38NWINLDD140 pKa = 4.14LGLTLRR146 pKa = 11.84KK147 pKa = 9.6YY148 pKa = 10.35DD149 pKa = 3.56GEE151 pKa = 4.35VRR153 pKa = 11.84LVTPATGSNLNEE165 pKa = 3.98FEE167 pKa = 4.67EE168 pKa = 5.49LDD170 pKa = 3.54FTLPLLYY177 pKa = 10.62ASGRR181 pKa = 11.84IDD183 pKa = 4.19LPFTGFFIDD192 pKa = 3.82GQLNIISYY200 pKa = 11.12DD201 pKa = 3.69DD202 pKa = 5.03DD203 pKa = 4.37SVSDD207 pKa = 3.27TSIAFGYY214 pKa = 9.93EE215 pKa = 4.06SDD217 pKa = 3.32WGLGAKK223 pKa = 9.65AGYY226 pKa = 10.68RR227 pKa = 11.84MFDD230 pKa = 3.4LDD232 pKa = 4.24VEE234 pKa = 4.26EE235 pKa = 6.0DD236 pKa = 3.69SFKK239 pKa = 11.43GDD241 pKa = 3.72LEE243 pKa = 4.07FDD245 pKa = 3.39GAYY248 pKa = 9.78ISVFYY253 pKa = 10.97HH254 pKa = 6.22FF255 pKa = 5.31

Molecular weight: 27.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A545UGR9|A0A545UGR9_9GAMM Threonine/serine exporter family protein OS=Aliikangiella sp. M105 OX=2592383 GN=FLL46_09095 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.3RR12 pKa = 11.84KK13 pKa = 9.12RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.47GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38NGRR28 pKa = 11.84QIINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5286

0

5286

2022666

30

13018

382.6

42.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.575 ± 0.029

0.952 ± 0.013

5.703 ± 0.043

6.449 ± 0.041

4.361 ± 0.024

6.592 ± 0.043

2.008 ± 0.016

6.677 ± 0.031

5.888 ± 0.046

9.873 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.062 ± 0.02

5.206 ± 0.034

3.696 ± 0.021

4.517 ± 0.029

4.393 ± 0.03

7.574 ± 0.039

5.435 ± 0.046

6.549 ± 0.036

1.249 ± 0.014

3.239 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski