Porcine transmissible gastroenteritis coronavirus (strain Purdue) (TGEV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Tegacovirus; Alphacoronavirus 1; Transmissible gastroenteritis virus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P09047|NS3B_CVPPU Putative non-structural protein 3b OS=Porcine transmissible gastroenteritis coronavirus (strain Purdue) OX=11151 GN=3b PE=5 SV=2
MM1 pKa = 8.02DD2 pKa = 4.08IVKK5 pKa = 10.35SIYY8 pKa = 9.68TSVDD12 pKa = 2.93AVLDD16 pKa = 4.08EE17 pKa = 4.83LDD19 pKa = 3.58CAYY22 pKa = 10.56FAVTLKK28 pKa = 11.12VEE30 pKa = 4.99FKK32 pKa = 9.86TGKK35 pKa = 10.29LLVCIGFGDD44 pKa = 3.78TLLAAKK50 pKa = 10.2DD51 pKa = 3.25KK52 pKa = 11.08AYY54 pKa = 10.71AKK56 pKa = 10.16LGLSIIEE63 pKa = 4.36EE64 pKa = 4.51VNSHH68 pKa = 5.7IVVV71 pKa = 3.59

Molecular weight: 7.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P07946|SPIKE_CVPPU Spike glycoprotein OS=Porcine transmissible gastroenteritis coronavirus (strain Purdue) OX=11151 GN=S PE=1 SV=2
MM1 pKa = 7.48LVFLHH6 pKa = 6.78AVFITVLILLLIGRR20 pKa = 11.84LQLLEE25 pKa = 4.34RR26 pKa = 11.84LLLNHH31 pKa = 6.54SFNLKK36 pKa = 8.48TVNDD40 pKa = 3.73FNILYY45 pKa = 10.18RR46 pKa = 11.84SLAEE50 pKa = 3.86TRR52 pKa = 11.84LLKK55 pKa = 10.7VVLRR59 pKa = 11.84VIFLVLLGFCCYY71 pKa = 10.28RR72 pKa = 11.84LLVTLMM78 pKa = 4.2

Molecular weight: 9.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

13267

71

6684

1474.1

164.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.527 ± 0.186

3.136 ± 0.344

5.306 ± 0.211

4.914 ± 0.391

5.683 ± 0.245

6.422 ± 0.119

1.651 ± 0.114

5.992 ± 0.239

6.301 ± 0.78

8.238 ± 0.512

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.299 ± 0.28

6.075 ± 0.333

3.286 ± 0.11

2.925 ± 0.336

3.196 ± 0.585

6.904 ± 0.426

6.068 ± 0.542

9.218 ± 0.349

1.176 ± 0.205

4.681 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski