Rosellinia necatrix partitivirus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Alphapartitivirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L8B8G3|L8B8G3_9VIRU RNA-dependent RNA polymerase OS=Rosellinia necatrix partitivirus 2 OX=859651 GN=RdRp PE=4 SV=1
MM1 pKa = 7.04STPSVTLSAFKK12 pKa = 10.67AAVAKK17 pKa = 10.67SGLEE21 pKa = 3.92DD22 pKa = 3.53QFLKK26 pKa = 10.2LTGLTSMDD34 pKa = 4.18EE35 pKa = 4.04LSADD39 pKa = 3.38NFNEE43 pKa = 4.08KK44 pKa = 10.2FGQADD49 pKa = 3.92SLTKK53 pKa = 10.63SILAPSAAPVPHH65 pKa = 6.22QEE67 pKa = 4.18QKK69 pKa = 10.96KK70 pKa = 9.56EE71 pKa = 3.78DD72 pKa = 3.78ADD74 pKa = 3.84AKK76 pKa = 10.39RR77 pKa = 11.84VSAPVSASDD86 pKa = 3.13FLYY89 pKa = 10.45GFKK92 pKa = 10.81HH93 pKa = 5.03MTIQYY98 pKa = 10.76ASRR101 pKa = 11.84KK102 pKa = 8.93KK103 pKa = 10.76NNTFLPSAFMMMYY116 pKa = 9.73IIHH119 pKa = 6.52EE120 pKa = 4.46MNVLLCEE127 pKa = 3.71HH128 pKa = 7.11FYY130 pKa = 10.84FKK132 pKa = 10.91RR133 pKa = 11.84EE134 pKa = 3.92APNYY138 pKa = 9.91HH139 pKa = 6.93PLLLRR144 pKa = 11.84TYY146 pKa = 10.29FGILFIIQTLRR157 pKa = 11.84AQHH160 pKa = 6.34AANNLFGDD168 pKa = 3.5QFEE171 pKa = 4.43FLEE174 pKa = 4.75KK175 pKa = 10.48FLDD178 pKa = 4.23AYY180 pKa = 9.99PVDD183 pKa = 4.2TLPIPAPLIHH193 pKa = 6.76IFKK196 pKa = 9.63SLCSSRR202 pKa = 11.84PEE204 pKa = 3.74IPQYY208 pKa = 11.3GYY210 pKa = 8.49VTPFVPEE217 pKa = 4.26EE218 pKa = 3.68LWGTQLKK225 pKa = 10.46DD226 pKa = 3.44SPANALYY233 pKa = 10.72ARR235 pKa = 11.84NFFPNIPYY243 pKa = 10.1ILSLYY248 pKa = 8.71ATLNSSTIKK257 pKa = 10.84GSVKK261 pKa = 10.29EE262 pKa = 4.08MNSYY266 pKa = 10.32LGNGTDD272 pKa = 3.82DD273 pKa = 3.39RR274 pKa = 11.84TFAGHH279 pKa = 7.5AYY281 pKa = 10.43DD282 pKa = 5.23KK283 pKa = 11.41DD284 pKa = 3.44PAQWTEE290 pKa = 3.44EE291 pKa = 3.79RR292 pKa = 11.84AFFLRR297 pKa = 11.84SPGMEE302 pKa = 3.86HH303 pKa = 6.94ALEE306 pKa = 4.61GTSSFNKK313 pKa = 10.3DD314 pKa = 2.89FFEE317 pKa = 4.4NRR319 pKa = 11.84FDD321 pKa = 3.81NLEE324 pKa = 3.99VPIPSTNDD332 pKa = 2.67KK333 pKa = 11.13VKK335 pKa = 10.58FVDD338 pKa = 3.53QFCYY342 pKa = 10.36LSKK345 pKa = 10.75NLAWFRR351 pKa = 11.84DD352 pKa = 3.65LVEE355 pKa = 4.4IASAAAKK362 pKa = 9.97FFEE365 pKa = 5.12GSGTLADD372 pKa = 4.68CSPVGPPVCQVQTTISNVDD391 pKa = 3.42LHH393 pKa = 6.07TAPTSISKK401 pKa = 10.6GKK403 pKa = 10.04SFYY406 pKa = 11.34AEE408 pKa = 3.42AHH410 pKa = 6.05MKK412 pKa = 9.01LTTTSRR418 pKa = 11.84TMDD421 pKa = 3.74QLSMIMASASHH432 pKa = 6.6LNLRR436 pKa = 11.84YY437 pKa = 9.91AHH439 pKa = 6.53ATHH442 pKa = 7.31SIQSRR447 pKa = 11.84TSGPFWSLNPIEE459 pKa = 5.3SSVTDD464 pKa = 3.98EE465 pKa = 4.22SSLIQVRR472 pKa = 11.84QTVKK476 pKa = 10.91KK477 pKa = 9.23MMKK480 pKa = 10.17SKK482 pKa = 10.74VV483 pKa = 3.28

Molecular weight: 54.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L8B8G3|L8B8G3_9VIRU RNA-dependent RNA polymerase OS=Rosellinia necatrix partitivirus 2 OX=859651 GN=RdRp PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.39NITDD6 pKa = 4.21LPFPSKK12 pKa = 10.54GVPQFRR18 pKa = 11.84DD19 pKa = 3.2ALPIHH24 pKa = 6.4GRR26 pKa = 11.84DD27 pKa = 3.4PEE29 pKa = 4.48VPNPVTDD36 pKa = 3.81ASNRR40 pKa = 11.84IIDD43 pKa = 3.72FALRR47 pKa = 11.84KK48 pKa = 9.67HH49 pKa = 5.35LTSDD53 pKa = 3.46EE54 pKa = 3.95FDD56 pKa = 3.29QVVNGYY62 pKa = 9.44RR63 pKa = 11.84RR64 pKa = 11.84SPWNEE69 pKa = 3.34DD70 pKa = 3.24ALNKK74 pKa = 10.49DD75 pKa = 3.32IEE77 pKa = 4.38KK78 pKa = 10.48LDD80 pKa = 3.9SDD82 pKa = 4.0EE83 pKa = 4.14HH84 pKa = 5.7TVIKK88 pKa = 10.1DD89 pKa = 3.11QHH91 pKa = 6.55YY92 pKa = 9.95EE93 pKa = 3.6NAIQHH98 pKa = 4.85VQKK101 pKa = 10.87LLTPEE106 pKa = 4.81KK107 pKa = 9.77PLQPVHH113 pKa = 6.42FADD116 pKa = 3.45LRR118 pKa = 11.84RR119 pKa = 11.84YY120 pKa = 9.02KK121 pKa = 9.91WRR123 pKa = 11.84LSTNIGAPFASSKK136 pKa = 10.0HH137 pKa = 3.74WQDD140 pKa = 3.24YY141 pKa = 9.83VKK143 pKa = 11.03AKK145 pKa = 10.38FNHH148 pKa = 5.76FRR150 pKa = 11.84DD151 pKa = 3.81GTPFEE156 pKa = 5.15NIAHH160 pKa = 6.99RR161 pKa = 11.84DD162 pKa = 3.69LFVEE166 pKa = 4.24AHH168 pKa = 7.09KK169 pKa = 10.74DD170 pKa = 3.7SQPLEE175 pKa = 3.92ITDD178 pKa = 4.3ARR180 pKa = 11.84MTKK183 pKa = 10.2HH184 pKa = 6.11NLYY187 pKa = 9.62TEE189 pKa = 4.45AFYY192 pKa = 11.22VSRR195 pKa = 11.84EE196 pKa = 4.03TIHH199 pKa = 6.95RR200 pKa = 11.84IKK202 pKa = 10.96DD203 pKa = 3.26GEE205 pKa = 4.31TTDD208 pKa = 5.02RR209 pKa = 11.84YY210 pKa = 11.34GNDD213 pKa = 2.54ARR215 pKa = 11.84YY216 pKa = 9.48WNTAFARR223 pKa = 11.84QHH225 pKa = 5.79LVKK228 pKa = 10.44ADD230 pKa = 3.74EE231 pKa = 4.48DD232 pKa = 4.15DD233 pKa = 3.54KK234 pKa = 11.81VRR236 pKa = 11.84LVFGAPFSLLCAEE249 pKa = 5.89LMFIWPLFIFLLSLKK264 pKa = 10.46GSTAFMLWTFEE275 pKa = 4.26TIIGGWYY282 pKa = 9.6RR283 pKa = 11.84LVNFFTTYY291 pKa = 11.19ALRR294 pKa = 11.84HH295 pKa = 4.97STVVTVDD302 pKa = 2.42WSGFDD307 pKa = 2.7RR308 pKa = 11.84YY309 pKa = 10.5ARR311 pKa = 11.84HH312 pKa = 5.35TVIKK316 pKa = 9.69DD317 pKa = 2.72IHH319 pKa = 6.87HH320 pKa = 6.72RR321 pKa = 11.84IIRR324 pKa = 11.84PMFDD328 pKa = 3.43FSHH331 pKa = 7.09GYY333 pKa = 9.97HH334 pKa = 6.63PTRR337 pKa = 11.84DD338 pKa = 3.71YY339 pKa = 11.54PDD341 pKa = 3.24TSKK344 pKa = 9.96TKK346 pKa = 10.49DD347 pKa = 3.38GQSNEE352 pKa = 3.59WRR354 pKa = 11.84ITNLWNWMTDD364 pKa = 3.61AILSTPLLLPNGRR377 pKa = 11.84FIRR380 pKa = 11.84FNHH383 pKa = 5.65SGIYY387 pKa = 9.67SGYY390 pKa = 9.65FQTQILDD397 pKa = 3.73SIYY400 pKa = 9.67NLVMIFTILSRR411 pKa = 11.84MGFDD415 pKa = 4.81LDD417 pKa = 3.44KK418 pKa = 11.28CVIKK422 pKa = 11.04VQGDD426 pKa = 3.43DD427 pKa = 4.1SIFMLLCCFIMISTSFFTLFKK448 pKa = 10.78YY449 pKa = 9.48YY450 pKa = 10.87AEE452 pKa = 4.53YY453 pKa = 11.21YY454 pKa = 10.57FGAKK458 pKa = 10.04LNEE461 pKa = 4.35KK462 pKa = 9.96KK463 pKa = 10.75SEE465 pKa = 3.97IRR467 pKa = 11.84PSLQDD472 pKa = 3.57AEE474 pKa = 4.34VLKK477 pKa = 10.69YY478 pKa = 10.43RR479 pKa = 11.84NRR481 pKa = 11.84NGIPYY486 pKa = 9.3RR487 pKa = 11.84DD488 pKa = 4.25RR489 pKa = 11.84ISLLAQLRR497 pKa = 11.84HH498 pKa = 5.62PEE500 pKa = 3.69RR501 pKa = 11.84RR502 pKa = 11.84TDD504 pKa = 3.35VDD506 pKa = 4.19SVAARR511 pKa = 11.84CIGIAYY517 pKa = 7.91AACGQDD523 pKa = 2.7ATVYY527 pKa = 9.32LICEE531 pKa = 4.7DD532 pKa = 3.28IYY534 pKa = 11.44NYY536 pKa = 10.27LVKK539 pKa = 10.49KK540 pKa = 10.07YY541 pKa = 9.71DD542 pKa = 2.93AHH544 pKa = 6.47ARR546 pKa = 11.84QGEE549 pKa = 4.26LDD551 pKa = 3.5FMFRR555 pKa = 11.84HH556 pKa = 6.29LEE558 pKa = 3.89LHH560 pKa = 6.25DD561 pKa = 4.23TVPSAATFPSWFDD574 pKa = 3.44TMAHH578 pKa = 5.88LTDD581 pKa = 4.47GPRR584 pKa = 11.84DD585 pKa = 3.74PVPSHH590 pKa = 6.49WPTDD594 pKa = 3.72YY595 pKa = 11.22FIGLPGRR602 pKa = 11.84LL603 pKa = 3.5

Molecular weight: 70.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1086

483

603

543.0

62.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.459 ± 0.986

1.105 ± 0.048

6.814 ± 1.26

4.604 ± 0.391

6.814 ± 0.154

4.236 ± 0.206

3.683 ± 0.536

5.709 ± 0.788

5.525 ± 0.469

8.84 ± 0.184

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.486 ± 0.423

4.236 ± 0.218

5.525 ± 0.327

3.223 ± 0.344

5.341 ± 1.385

7.459 ± 1.693

6.538 ± 0.06

4.696 ± 0.096

1.565 ± 0.503

4.144 ± 0.426
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski