Cactus virus X

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q91ND7|Q91ND7_9VIRU 8 kDa protein OS=Cactus virus X OX=112227 PE=4 SV=1
MM1 pKa = 7.01EE2 pKa = 5.12TLTALLIANGYY13 pKa = 6.89TRR15 pKa = 11.84TDD17 pKa = 3.59LPISKK22 pKa = 9.24PLVIHH27 pKa = 6.62AVAGAGKK34 pKa = 6.7TTLIRR39 pKa = 11.84QFLHH43 pKa = 4.98QHH45 pKa = 5.7SATNAQTLGTPDD57 pKa = 3.77KK58 pKa = 10.51PNLSRR63 pKa = 11.84KK64 pKa = 8.56MIRR67 pKa = 11.84QFSMPKK73 pKa = 9.88ANHH76 pKa = 5.96FNILDD81 pKa = 4.02EE82 pKa = 4.59YY83 pKa = 10.65CAQPLKK89 pKa = 10.81GSWDD93 pKa = 3.48AVFADD98 pKa = 4.67PLQHH102 pKa = 7.14PDD104 pKa = 3.32YY105 pKa = 10.84ALEE108 pKa = 3.72PHH110 pKa = 6.98FIKK113 pKa = 9.95EE114 pKa = 4.2TSHH117 pKa = 6.77RR118 pKa = 11.84LGPSTCEE125 pKa = 4.43LISSLGILIYY135 pKa = 10.08PDD137 pKa = 4.64SEE139 pKa = 4.43DD140 pKa = 3.44QVVTRR145 pKa = 11.84KK146 pKa = 10.15GVFEE150 pKa = 4.32SEE152 pKa = 4.09LFGVVIALDD161 pKa = 3.53EE162 pKa = 5.56AIFQLASRR170 pKa = 11.84HH171 pKa = 4.87GLKK174 pKa = 10.13PLCPKK179 pKa = 9.66ATIGLQFPVVTVLSSLALEE198 pKa = 4.11QVEE201 pKa = 5.23DD202 pKa = 3.69STALYY207 pKa = 10.04IALTRR212 pKa = 11.84HH213 pKa = 4.96TKK215 pKa = 9.25EE216 pKa = 3.6LHH218 pKa = 5.31VRR220 pKa = 11.84CPTEE224 pKa = 4.8ADD226 pKa = 3.08ATTT229 pKa = 3.47

Molecular weight: 25.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q91ND8|Q91ND8_9VIRU 15 kDa protein OS=Cactus virus X OX=112227 PE=4 SV=1
MM1 pKa = 7.59PHH3 pKa = 6.32TATGRR8 pKa = 11.84TWRR11 pKa = 11.84TCSEE15 pKa = 3.89STQRR19 pKa = 11.84LRR21 pKa = 11.84DD22 pKa = 3.66APTSPWTPPFFPAGICSSHH41 pKa = 6.04PCSRR45 pKa = 11.84NKK47 pKa = 9.02HH48 pKa = 4.87TGSWDD53 pKa = 3.21TRR55 pKa = 11.84FQPTPGVKK63 pKa = 9.43EE64 pKa = 4.05SRR66 pKa = 11.84RR67 pKa = 11.84MRR69 pKa = 11.84SKK71 pKa = 10.72YY72 pKa = 10.54

Molecular weight: 8.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

2376

64

1543

339.4

38.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.987 ± 0.53

1.557 ± 0.254

4.167 ± 0.698

5.303 ± 0.879

4.63 ± 0.538

5.429 ± 0.415

3.62 ± 0.389

5.219 ± 0.709

6.061 ± 1.071

10.101 ± 1.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.231 ± 0.395

4.125 ± 0.474

7.323 ± 0.835

4.335 ± 0.67

4.714 ± 0.951

7.786 ± 1.482

7.997 ± 0.736

4.461 ± 0.558

1.473 ± 0.237

2.483 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski