Amino acid dipepetide frequency for Microbacterium phage Dewdrop

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.206AlaAla: 13.206 ± 1.166
0.395AlaCys: 0.395 ± 0.119
5.137AlaAsp: 5.137 ± 0.415
8.76AlaGlu: 8.76 ± 0.674
3.688AlaPhe: 3.688 ± 0.357
7.937AlaGly: 7.937 ± 0.804
1.943AlaHis: 1.943 ± 0.288
4.577AlaIle: 4.577 ± 0.476
3.82AlaLys: 3.82 ± 0.411
9.254AlaLeu: 9.254 ± 0.546
2.338AlaMet: 2.338 ± 0.264
2.503AlaAsn: 2.503 ± 0.291
5.467AlaPro: 5.467 ± 0.508
5.039AlaGln: 5.039 ± 0.486
5.928AlaArg: 5.928 ± 0.426
5.631AlaSer: 5.631 ± 0.555
6.586AlaThr: 6.586 ± 0.601
7.08AlaVal: 7.08 ± 0.566
2.47AlaTrp: 2.47 ± 0.316
2.47AlaTyr: 2.47 ± 0.268
0.0AlaXaa: 0.0 ± 0.0
Cys
0.79CysAla: 0.79 ± 0.19
0.099CysCys: 0.099 ± 0.058
0.593CysAsp: 0.593 ± 0.167
0.692CysGlu: 0.692 ± 0.145
0.329CysPhe: 0.329 ± 0.117
1.218CysGly: 1.218 ± 0.218
0.198CysHis: 0.198 ± 0.073
0.296CysIle: 0.296 ± 0.086
0.263CysLys: 0.263 ± 0.113
0.494CysLeu: 0.494 ± 0.14
0.165CysMet: 0.165 ± 0.073
0.198CysAsn: 0.198 ± 0.102
0.659CysPro: 0.659 ± 0.132
0.263CysGln: 0.263 ± 0.09
0.231CysArg: 0.231 ± 0.084
0.329CysSer: 0.329 ± 0.116
0.593CysThr: 0.593 ± 0.179
0.428CysVal: 0.428 ± 0.109
0.099CysTrp: 0.099 ± 0.05
0.132CysTyr: 0.132 ± 0.068
0.0CysXaa: 0.0 ± 0.0
Asp
5.664AspAla: 5.664 ± 0.399
0.757AspCys: 0.757 ± 0.166
5.137AspAsp: 5.137 ± 0.554
5.104AspGlu: 5.104 ± 0.522
1.976AspPhe: 1.976 ± 0.21
6.817AspGly: 6.817 ± 0.443
1.383AspHis: 1.383 ± 0.242
2.635AspIle: 2.635 ± 0.337
1.712AspLys: 1.712 ± 0.263
4.709AspLeu: 4.709 ± 0.384
1.383AspMet: 1.383 ± 0.201
1.482AspAsn: 1.482 ± 0.205
4.281AspPro: 4.281 ± 0.406
1.811AspGln: 1.811 ± 0.223
4.215AspArg: 4.215 ± 0.471
2.832AspSer: 2.832 ± 0.305
3.359AspThr: 3.359 ± 0.333
4.347AspVal: 4.347 ± 0.418
1.614AspTrp: 1.614 ± 0.269
1.91AspTyr: 1.91 ± 0.328
0.0AspXaa: 0.0 ± 0.0
Glu
8.398GluAla: 8.398 ± 0.618
0.593GluCys: 0.593 ± 0.149
4.512GluAsp: 4.512 ± 0.43
6.652GluGlu: 6.652 ± 0.712
2.009GluPhe: 2.009 ± 0.29
5.368GluGly: 5.368 ± 0.458
1.449GluHis: 1.449 ± 0.292
2.865GluIle: 2.865 ± 0.277
4.084GluLys: 4.084 ± 0.45
6.422GluLeu: 6.422 ± 0.571
2.305GluMet: 2.305 ± 0.237
2.47GluAsn: 2.47 ± 0.299
3.721GluPro: 3.721 ± 0.381
3.787GluGln: 3.787 ± 0.441
5.895GluArg: 5.895 ± 0.547
3.524GluSer: 3.524 ± 0.372
3.886GluThr: 3.886 ± 0.49
5.829GluVal: 5.829 ± 0.419
1.548GluTrp: 1.548 ± 0.245
1.976GluTyr: 1.976 ± 0.216
0.0GluXaa: 0.0 ± 0.0
Phe
2.733PheAla: 2.733 ± 0.337
0.231PheCys: 0.231 ± 0.074
2.042PheAsp: 2.042 ± 0.285
2.272PheGlu: 2.272 ± 0.298
0.79PhePhe: 0.79 ± 0.17
2.997PheGly: 2.997 ± 0.385
0.395PheHis: 0.395 ± 0.132
1.449PheIle: 1.449 ± 0.315
0.626PheLys: 0.626 ± 0.127
2.371PheLeu: 2.371 ± 0.264
0.593PheMet: 0.593 ± 0.132
0.692PheAsn: 0.692 ± 0.156
1.284PhePro: 1.284 ± 0.228
1.251PheGln: 1.251 ± 0.198
1.976PheArg: 1.976 ± 0.283
1.844PheSer: 1.844 ± 0.286
2.371PheThr: 2.371 ± 0.256
1.943PheVal: 1.943 ± 0.329
0.889PheTrp: 0.889 ± 0.202
0.823PheTyr: 0.823 ± 0.178
0.0PheXaa: 0.0 ± 0.0
Gly
7.377GlyAla: 7.377 ± 0.799
0.626GlyCys: 0.626 ± 0.188
4.479GlyAsp: 4.479 ± 0.352
6.323GlyGlu: 6.323 ± 0.523
2.964GlyPhe: 2.964 ± 0.347
7.541GlyGly: 7.541 ± 0.819
1.745GlyHis: 1.745 ± 0.268
3.293GlyIle: 3.293 ± 0.442
3.194GlyLys: 3.194 ± 0.359
6.059GlyLeu: 6.059 ± 0.58
2.338GlyMet: 2.338 ± 0.27
2.338GlyAsn: 2.338 ± 0.288
3.82GlyPro: 3.82 ± 0.3
3.03GlyGln: 3.03 ± 0.291
5.401GlyArg: 5.401 ± 0.476
5.236GlySer: 5.236 ± 0.686
5.829GlyThr: 5.829 ± 0.624
5.269GlyVal: 5.269 ± 0.401
2.239GlyTrp: 2.239 ± 0.276
2.766GlyTyr: 2.766 ± 0.357
0.0GlyXaa: 0.0 ± 0.0
His
1.844HisAla: 1.844 ± 0.334
0.066HisCys: 0.066 ± 0.048
1.218HisAsp: 1.218 ± 0.161
1.614HisGlu: 1.614 ± 0.218
0.461HisPhe: 0.461 ± 0.118
1.68HisGly: 1.68 ± 0.229
0.231HisHis: 0.231 ± 0.087
0.988HisIle: 0.988 ± 0.165
0.527HisLys: 0.527 ± 0.113
1.581HisLeu: 1.581 ± 0.222
0.231HisMet: 0.231 ± 0.08
0.527HisAsn: 0.527 ± 0.132
1.218HisPro: 1.218 ± 0.227
0.724HisGln: 0.724 ± 0.159
1.712HisArg: 1.712 ± 0.298
0.823HisSer: 0.823 ± 0.17
1.12HisThr: 1.12 ± 0.184
1.482HisVal: 1.482 ± 0.209
0.329HisTrp: 0.329 ± 0.105
0.856HisTyr: 0.856 ± 0.159
0.0HisXaa: 0.0 ± 0.0
Ile
4.775IleAla: 4.775 ± 0.52
0.231IleCys: 0.231 ± 0.097
4.018IleAsp: 4.018 ± 0.355
3.524IleGlu: 3.524 ± 0.385
0.955IlePhe: 0.955 ± 0.219
3.227IleGly: 3.227 ± 0.366
0.955IleHis: 0.955 ± 0.175
1.581IleIle: 1.581 ± 0.256
0.922IleLys: 0.922 ± 0.169
2.733IleLeu: 2.733 ± 0.314
0.988IleMet: 0.988 ± 0.194
0.988IleAsn: 0.988 ± 0.178
2.799IlePro: 2.799 ± 0.292
2.042IleGln: 2.042 ± 0.253
2.997IleArg: 2.997 ± 0.283
2.536IleSer: 2.536 ± 0.435
3.293IleThr: 3.293 ± 0.367
3.557IleVal: 3.557 ± 0.417
1.218IleTrp: 1.218 ± 0.264
1.186IleTyr: 1.186 ± 0.241
0.0IleXaa: 0.0 ± 0.0
Lys
5.137LysAla: 5.137 ± 0.475
0.296LysCys: 0.296 ± 0.092
1.844LysAsp: 1.844 ± 0.273
2.404LysGlu: 2.404 ± 0.312
0.823LysPhe: 0.823 ± 0.165
2.536LysGly: 2.536 ± 0.302
0.823LysHis: 0.823 ± 0.171
1.712LysIle: 1.712 ± 0.23
1.68LysLys: 1.68 ± 0.272
2.733LysLeu: 2.733 ± 0.294
0.955LysMet: 0.955 ± 0.164
1.054LysAsn: 1.054 ± 0.203
2.437LysPro: 2.437 ± 0.328
1.383LysGln: 1.383 ± 0.192
2.766LysArg: 2.766 ± 0.31
1.811LysSer: 1.811 ± 0.262
2.009LysThr: 2.009 ± 0.264
2.305LysVal: 2.305 ± 0.269
0.724LysTrp: 0.724 ± 0.141
0.823LysTyr: 0.823 ± 0.165
0.0LysXaa: 0.0 ± 0.0
Leu
7.212LeuAla: 7.212 ± 0.749
0.692LeuCys: 0.692 ± 0.152
5.697LeuAsp: 5.697 ± 0.485
6.586LeuGlu: 6.586 ± 0.499
1.35LeuPhe: 1.35 ± 0.203
5.598LeuGly: 5.598 ± 0.404
1.91LeuHis: 1.91 ± 0.323
3.491LeuIle: 3.491 ± 0.391
2.997LeuLys: 2.997 ± 0.311
5.895LeuLeu: 5.895 ± 0.529
2.108LeuMet: 2.108 ± 0.235
2.667LeuAsn: 2.667 ± 0.251
4.94LeuPro: 4.94 ± 0.451
2.47LeuGln: 2.47 ± 0.345
5.302LeuArg: 5.302 ± 0.526
5.104LeuSer: 5.104 ± 0.472
5.17LeuThr: 5.17 ± 0.431
5.467LeuVal: 5.467 ± 0.406
1.614LeuTrp: 1.614 ± 0.248
1.976LeuTyr: 1.976 ± 0.249
0.0LeuXaa: 0.0 ± 0.0
Met
3.03MetAla: 3.03 ± 0.272
0.132MetCys: 0.132 ± 0.07
1.416MetAsp: 1.416 ± 0.213
1.449MetGlu: 1.449 ± 0.222
0.823MetPhe: 0.823 ± 0.155
1.811MetGly: 1.811 ± 0.29
0.593MetHis: 0.593 ± 0.164
0.988MetIle: 0.988 ± 0.167
1.317MetLys: 1.317 ± 0.221
1.284MetLeu: 1.284 ± 0.204
0.889MetMet: 0.889 ± 0.17
1.218MetAsn: 1.218 ± 0.192
1.778MetPro: 1.778 ± 0.25
0.56MetGln: 0.56 ± 0.128
1.877MetArg: 1.877 ± 0.318
2.503MetSer: 2.503 ± 0.297
2.173MetThr: 2.173 ± 0.27
1.515MetVal: 1.515 ± 0.216
0.362MetTrp: 0.362 ± 0.099
0.461MetTyr: 0.461 ± 0.118
0.0MetXaa: 0.0 ± 0.0
Asn
2.898AsnAla: 2.898 ± 0.349
0.231AsnCys: 0.231 ± 0.094
1.976AsnAsp: 1.976 ± 0.294
2.569AsnGlu: 2.569 ± 0.313
0.626AsnPhe: 0.626 ± 0.153
2.997AsnGly: 2.997 ± 0.369
0.659AsnHis: 0.659 ± 0.141
1.317AsnIle: 1.317 ± 0.262
0.494AsnLys: 0.494 ± 0.115
2.206AsnLeu: 2.206 ± 0.294
0.659AsnMet: 0.659 ± 0.129
1.054AsnAsn: 1.054 ± 0.204
2.47AsnPro: 2.47 ± 0.341
1.021AsnGln: 1.021 ± 0.195
1.811AsnArg: 1.811 ± 0.229
1.251AsnSer: 1.251 ± 0.197
1.482AsnThr: 1.482 ± 0.246
2.667AsnVal: 2.667 ± 0.295
0.856AsnTrp: 0.856 ± 0.186
0.593AsnTyr: 0.593 ± 0.139
0.0AsnXaa: 0.0 ± 0.0
Pro
5.104ProAla: 5.104 ± 0.561
0.461ProCys: 0.461 ± 0.144
3.491ProAsp: 3.491 ± 0.335
5.071ProGlu: 5.071 ± 0.467
1.91ProPhe: 1.91 ± 0.248
4.577ProGly: 4.577 ± 0.57
1.054ProHis: 1.054 ± 0.199
2.997ProIle: 2.997 ± 0.341
2.141ProLys: 2.141 ± 0.283
3.524ProLeu: 3.524 ± 0.376
1.515ProMet: 1.515 ± 0.212
1.712ProAsn: 1.712 ± 0.229
2.964ProPro: 2.964 ± 0.464
1.647ProGln: 1.647 ± 0.286
3.096ProArg: 3.096 ± 0.303
3.26ProSer: 3.26 ± 0.438
4.38ProThr: 4.38 ± 0.489
4.479ProVal: 4.479 ± 0.352
1.087ProTrp: 1.087 ± 0.217
1.186ProTyr: 1.186 ± 0.247
0.0ProXaa: 0.0 ± 0.0
Gln
3.491GlnAla: 3.491 ± 0.339
0.362GlnCys: 0.362 ± 0.102
2.075GlnAsp: 2.075 ± 0.266
3.096GlnGlu: 3.096 ± 0.372
0.889GlnPhe: 0.889 ± 0.22
2.898GlnGly: 2.898 ± 0.385
0.79GlnHis: 0.79 ± 0.13
2.009GlnIle: 2.009 ± 0.245
1.482GlnLys: 1.482 ± 0.235
3.491GlnLeu: 3.491 ± 0.403
0.922GlnMet: 0.922 ± 0.224
0.988GlnAsn: 0.988 ± 0.157
1.778GlnPro: 1.778 ± 0.269
1.515GlnGln: 1.515 ± 0.256
2.931GlnArg: 2.931 ± 0.394
1.943GlnSer: 1.943 ± 0.258
2.503GlnThr: 2.503 ± 0.307
2.832GlnVal: 2.832 ± 0.295
0.889GlnTrp: 0.889 ± 0.154
0.724GlnTyr: 0.724 ± 0.161
0.0GlnXaa: 0.0 ± 0.0
Arg
8.299ArgAla: 8.299 ± 0.513
0.527ArgCys: 0.527 ± 0.121
3.853ArgAsp: 3.853 ± 0.408
5.006ArgGlu: 5.006 ± 0.463
2.173ArgPhe: 2.173 ± 0.302
5.071ArgGly: 5.071 ± 0.469
1.186ArgHis: 1.186 ± 0.196
3.096ArgIle: 3.096 ± 0.314
2.404ArgLys: 2.404 ± 0.315
6.026ArgLeu: 6.026 ± 0.494
2.206ArgMet: 2.206 ± 0.249
1.877ArgAsn: 1.877 ± 0.247
3.096ArgPro: 3.096 ± 0.352
2.667ArgGln: 2.667 ± 0.307
5.664ArgArg: 5.664 ± 0.505
2.997ArgSer: 2.997 ± 0.323
3.293ArgThr: 3.293 ± 0.34
4.61ArgVal: 4.61 ± 0.464
1.712ArgTrp: 1.712 ± 0.243
1.811ArgTyr: 1.811 ± 0.252
0.0ArgXaa: 0.0 ± 0.0
Ser
5.368SerAla: 5.368 ± 0.574
0.494SerCys: 0.494 ± 0.134
3.557SerAsp: 3.557 ± 0.34
3.853SerGlu: 3.853 ± 0.52
1.844SerPhe: 1.844 ± 0.224
5.17SerGly: 5.17 ± 0.461
0.692SerHis: 0.692 ± 0.164
2.47SerIle: 2.47 ± 0.313
1.811SerLys: 1.811 ± 0.225
4.676SerLeu: 4.676 ± 0.374
2.042SerMet: 2.042 ± 0.23
1.745SerAsn: 1.745 ± 0.252
2.338SerPro: 2.338 ± 0.322
1.811SerGln: 1.811 ± 0.209
3.524SerArg: 3.524 ± 0.327
2.733SerSer: 2.733 ± 0.335
3.26SerThr: 3.26 ± 0.349
4.149SerVal: 4.149 ± 0.409
1.416SerTrp: 1.416 ± 0.277
1.68SerTyr: 1.68 ± 0.258
0.0SerXaa: 0.0 ± 0.0
Thr
6.949ThrAla: 6.949 ± 0.666
0.856ThrCys: 0.856 ± 0.234
3.59ThrAsp: 3.59 ± 0.313
4.479ThrGlu: 4.479 ± 0.4
1.778ThrPhe: 1.778 ± 0.249
5.829ThrGly: 5.829 ± 0.528
0.955ThrHis: 0.955 ± 0.18
3.59ThrIle: 3.59 ± 0.336
1.976ThrLys: 1.976 ± 0.357
5.039ThrLeu: 5.039 ± 0.428
1.383ThrMet: 1.383 ± 0.218
1.68ThrAsn: 1.68 ± 0.259
4.149ThrPro: 4.149 ± 0.478
1.943ThrGln: 1.943 ± 0.285
3.458ThrArg: 3.458 ± 0.358
3.787ThrSer: 3.787 ± 0.384
4.775ThrThr: 4.775 ± 0.554
5.565ThrVal: 5.565 ± 0.472
1.35ThrTrp: 1.35 ± 0.254
1.647ThrTyr: 1.647 ± 0.253
0.0ThrXaa: 0.0 ± 0.0
Val
7.541ValAla: 7.541 ± 0.664
0.494ValCys: 0.494 ± 0.136
5.302ValAsp: 5.302 ± 0.43
4.742ValGlu: 4.742 ± 0.503
1.943ValPhe: 1.943 ± 0.227
4.808ValGly: 4.808 ± 0.524
1.087ValHis: 1.087 ± 0.169
3.359ValIle: 3.359 ± 0.407
3.194ValLys: 3.194 ± 0.309
5.928ValLeu: 5.928 ± 0.501
1.844ValMet: 1.844 ± 0.271
2.503ValAsn: 2.503 ± 0.295
4.314ValPro: 4.314 ± 0.452
2.536ValGln: 2.536 ± 0.292
5.071ValArg: 5.071 ± 0.398
3.688ValSer: 3.688 ± 0.293
5.928ValThr: 5.928 ± 0.497
5.434ValVal: 5.434 ± 0.44
1.647ValTrp: 1.647 ± 0.24
1.317ValTyr: 1.317 ± 0.222
0.0ValXaa: 0.0 ± 0.0
Trp
2.075TrpAla: 2.075 ± 0.321
0.231TrpCys: 0.231 ± 0.084
1.482TrpAsp: 1.482 ± 0.275
1.383TrpGlu: 1.383 ± 0.229
1.284TrpPhe: 1.284 ± 0.178
1.515TrpGly: 1.515 ± 0.226
0.626TrpHis: 0.626 ± 0.125
1.12TrpIle: 1.12 ± 0.236
0.757TrpLys: 0.757 ± 0.166
1.647TrpLeu: 1.647 ± 0.244
0.56TrpMet: 0.56 ± 0.135
1.087TrpAsn: 1.087 ± 0.371
1.087TrpPro: 1.087 ± 0.207
0.955TrpGln: 0.955 ± 0.194
1.482TrpArg: 1.482 ± 0.212
1.515TrpSer: 1.515 ± 0.233
1.317TrpThr: 1.317 ± 0.236
2.206TrpVal: 2.206 ± 0.296
0.461TrpTrp: 0.461 ± 0.129
0.428TrpTyr: 0.428 ± 0.133
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.338TyrAla: 2.338 ± 0.309
0.296TyrCys: 0.296 ± 0.098
1.712TyrAsp: 1.712 ± 0.289
1.712TyrGlu: 1.712 ± 0.237
0.955TyrPhe: 0.955 ± 0.159
2.272TyrGly: 2.272 ± 0.268
0.56TyrHis: 0.56 ± 0.147
0.692TyrIle: 0.692 ± 0.117
0.856TyrLys: 0.856 ± 0.167
2.173TyrLeu: 2.173 ± 0.252
0.724TyrMet: 0.724 ± 0.17
1.054TyrAsn: 1.054 ± 0.212
1.087TyrPro: 1.087 ± 0.183
1.153TyrGln: 1.153 ± 0.211
2.239TyrArg: 2.239 ± 0.227
1.284TyrSer: 1.284 ± 0.223
1.449TyrThr: 1.449 ± 0.223
1.515TyrVal: 1.515 ± 0.27
0.692TyrTrp: 0.692 ± 0.161
0.659TyrTyr: 0.659 ± 0.177
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 156 proteins (30367 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski