Amino acid dipepetide frequency for Leucobacter sp. HDW9A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.722AlaAla: 16.722 ± 0.221
0.706AlaCys: 0.706 ± 0.035
6.75AlaAsp: 6.75 ± 0.105
8.135AlaGlu: 8.135 ± 0.144
3.763AlaPhe: 3.763 ± 0.081
10.609AlaGly: 10.609 ± 0.162
2.111AlaHis: 2.111 ± 0.066
5.942AlaIle: 5.942 ± 0.117
3.533AlaLys: 3.533 ± 0.078
12.53AlaLeu: 12.53 ± 0.182
2.487AlaMet: 2.487 ± 0.075
2.929AlaAsn: 2.929 ± 0.073
5.381AlaPro: 5.381 ± 0.101
3.937AlaGln: 3.937 ± 0.078
7.191AlaArg: 7.191 ± 0.116
7.113AlaSer: 7.113 ± 0.103
7.37AlaThr: 7.37 ± 0.126
10.053AlaVal: 10.053 ± 0.125
1.531AlaTrp: 1.531 ± 0.052
2.169AlaTyr: 2.169 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
0.706CysAla: 0.706 ± 0.036
0.071CysCys: 0.071 ± 0.012
0.376CysAsp: 0.376 ± 0.022
0.383CysGlu: 0.383 ± 0.026
0.164CysPhe: 0.164 ± 0.016
0.627CysGly: 0.627 ± 0.031
0.121CysHis: 0.121 ± 0.013
0.219CysIle: 0.219 ± 0.015
0.12CysLys: 0.12 ± 0.011
0.547CysLeu: 0.547 ± 0.026
0.12CysMet: 0.12 ± 0.012
0.141CysAsn: 0.141 ± 0.014
0.355CysPro: 0.355 ± 0.027
0.155CysGln: 0.155 ± 0.017
0.386CysArg: 0.386 ± 0.025
0.452CysSer: 0.452 ± 0.027
0.497CysThr: 0.497 ± 0.029
0.557CysVal: 0.557 ± 0.032
0.088CysTrp: 0.088 ± 0.013
0.121CysTyr: 0.121 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
6.667AspAla: 6.667 ± 0.108
0.285AspCys: 0.285 ± 0.02
2.982AspAsp: 2.982 ± 0.083
3.666AspGlu: 3.666 ± 0.086
1.757AspPhe: 1.757 ± 0.047
5.051AspGly: 5.051 ± 0.093
1.097AspHis: 1.097 ± 0.044
2.314AspIle: 2.314 ± 0.071
1.225AspLys: 1.225 ± 0.047
5.348AspLeu: 5.348 ± 0.099
0.862AspMet: 0.862 ± 0.034
1.292AspAsn: 1.292 ± 0.044
4.189AspPro: 4.189 ± 0.073
1.75AspGln: 1.75 ± 0.051
3.902AspArg: 3.902 ± 0.086
3.18AspSer: 3.18 ± 0.072
3.192AspThr: 3.192 ± 0.074
4.369AspVal: 4.369 ± 0.086
0.824AspTrp: 0.824 ± 0.038
1.227AspTyr: 1.227 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
7.439GluAla: 7.439 ± 0.135
0.379GluCys: 0.379 ± 0.025
2.737GluAsp: 2.737 ± 0.063
3.319GluGlu: 3.319 ± 0.085
2.028GluPhe: 2.028 ± 0.054
4.09GluGly: 4.09 ± 0.081
1.385GluHis: 1.385 ± 0.047
3.029GluIle: 3.029 ± 0.069
1.763GluLys: 1.763 ± 0.061
6.929GluLeu: 6.929 ± 0.139
1.158GluMet: 1.158 ± 0.04
1.555GluAsn: 1.555 ± 0.048
2.764GluPro: 2.764 ± 0.072
2.697GluGln: 2.697 ± 0.069
4.745GluArg: 4.745 ± 0.12
3.499GluSer: 3.499 ± 0.072
3.528GluThr: 3.528 ± 0.074
5.025GluVal: 5.025 ± 0.079
0.838GluTrp: 0.838 ± 0.034
1.181GluTyr: 1.181 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
4.25PheAla: 4.25 ± 0.084
0.198PheCys: 0.198 ± 0.018
2.274PheAsp: 2.274 ± 0.058
2.054PheGlu: 2.054 ± 0.061
1.122PhePhe: 1.122 ± 0.041
3.503PheGly: 3.503 ± 0.089
0.515PheHis: 0.515 ± 0.027
1.35PheIle: 1.35 ± 0.046
0.667PheLys: 0.667 ± 0.032
2.764PheLeu: 2.764 ± 0.075
0.618PheMet: 0.618 ± 0.03
0.763PheAsn: 0.763 ± 0.032
1.445PhePro: 1.445 ± 0.047
0.902PheGln: 0.902 ± 0.038
1.693PheArg: 1.693 ± 0.053
2.264PheSer: 2.264 ± 0.061
2.468PheThr: 2.468 ± 0.073
3.081PheVal: 3.081 ± 0.075
0.487PheTrp: 0.487 ± 0.024
0.755PheTyr: 0.755 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
9.263GlyAla: 9.263 ± 0.155
0.618GlyCys: 0.618 ± 0.029
4.739GlyAsp: 4.739 ± 0.09
5.242GlyGlu: 5.242 ± 0.093
3.415GlyPhe: 3.415 ± 0.078
7.183GlyGly: 7.183 ± 0.15
1.472GlyHis: 1.472 ± 0.05
4.883GlyIle: 4.883 ± 0.095
2.84GlyLys: 2.84 ± 0.074
8.316GlyLeu: 8.316 ± 0.121
1.935GlyMet: 1.935 ± 0.058
2.464GlyAsn: 2.464 ± 0.1
3.281GlyPro: 3.281 ± 0.068
2.75GlyGln: 2.75 ± 0.072
4.91GlyArg: 4.91 ± 0.085
6.279GlySer: 6.279 ± 0.116
5.74GlyThr: 5.74 ± 0.151
7.872GlyVal: 7.872 ± 0.099
1.494GlyTrp: 1.494 ± 0.054
2.164GlyTyr: 2.164 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
1.946HisAla: 1.946 ± 0.054
0.149HisCys: 0.149 ± 0.014
1.062HisAsp: 1.062 ± 0.036
1.2HisGlu: 1.2 ± 0.047
0.567HisPhe: 0.567 ± 0.027
1.63HisGly: 1.63 ± 0.052
0.458HisHis: 0.458 ± 0.026
0.67HisIle: 0.67 ± 0.036
0.432HisLys: 0.432 ± 0.024
1.991HisLeu: 1.991 ± 0.054
0.327HisMet: 0.327 ± 0.021
0.436HisAsn: 0.436 ± 0.023
1.311HisPro: 1.311 ± 0.044
0.599HisGln: 0.599 ± 0.03
1.318HisArg: 1.318 ± 0.046
1.151HisSer: 1.151 ± 0.036
1.208HisThr: 1.208 ± 0.05
1.272HisVal: 1.272 ± 0.046
0.281HisTrp: 0.281 ± 0.019
0.436HisTyr: 0.436 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
6.999IleAla: 6.999 ± 0.121
0.309IleCys: 0.309 ± 0.02
3.315IleAsp: 3.315 ± 0.073
3.251IleGlu: 3.251 ± 0.071
1.335IlePhe: 1.335 ± 0.048
4.494IleGly: 4.494 ± 0.081
0.732IleHis: 0.732 ± 0.031
2.012IleIle: 2.012 ± 0.065
1.021IleLys: 1.021 ± 0.038
4.294IleLeu: 4.294 ± 0.084
0.767IleMet: 0.767 ± 0.033
1.197IleAsn: 1.197 ± 0.042
2.539IlePro: 2.539 ± 0.06
1.15IleGln: 1.15 ± 0.042
2.622IleArg: 2.622 ± 0.071
2.956IleSer: 2.956 ± 0.069
3.348IleThr: 3.348 ± 0.081
4.536IleVal: 4.536 ± 0.089
0.544IleTrp: 0.544 ± 0.029
0.905IleTyr: 0.905 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
2.812LysAla: 2.812 ± 0.076
0.117LysCys: 0.117 ± 0.012
1.452LysAsp: 1.452 ± 0.054
1.349LysGlu: 1.349 ± 0.055
0.782LysPhe: 0.782 ± 0.035
1.884LysGly: 1.884 ± 0.053
0.613LysHis: 0.613 ± 0.031
1.412LysIle: 1.412 ± 0.049
1.195LysLys: 1.195 ± 0.048
2.981LysLeu: 2.981 ± 0.073
0.581LysMet: 0.581 ± 0.024
0.943LysAsn: 0.943 ± 0.04
1.712LysPro: 1.712 ± 0.056
1.171LysGln: 1.171 ± 0.037
2.04LysArg: 2.04 ± 0.055
1.682LysSer: 1.682 ± 0.048
1.91LysThr: 1.91 ± 0.057
2.246LysVal: 2.246 ± 0.076
0.33LysTrp: 0.33 ± 0.022
0.571LysTyr: 0.571 ± 0.029
0.0LysXaa: 0.0 ± 0.0
Leu
12.938LeuAla: 12.938 ± 0.174
0.582LeuCys: 0.582 ± 0.031
5.686LeuAsp: 5.686 ± 0.114
5.483LeuGlu: 5.483 ± 0.106
2.997LeuPhe: 2.997 ± 0.082
9.278LeuGly: 9.278 ± 0.152
1.692LeuHis: 1.692 ± 0.046
4.898LeuIle: 4.898 ± 0.109
2.495LeuLys: 2.495 ± 0.068
9.981LeuLeu: 9.981 ± 0.158
1.788LeuMet: 1.788 ± 0.055
2.467LeuAsn: 2.467 ± 0.056
5.21LeuPro: 5.21 ± 0.08
2.872LeuGln: 2.872 ± 0.065
6.872LeuArg: 6.872 ± 0.127
6.936LeuSer: 6.936 ± 0.101
6.613LeuThr: 6.613 ± 0.101
8.559LeuVal: 8.559 ± 0.133
1.158LeuTrp: 1.158 ± 0.038
1.628LeuTyr: 1.628 ± 0.052
0.0LeuXaa: 0.0 ± 0.0
Met
2.008MetAla: 2.008 ± 0.057
0.124MetCys: 0.124 ± 0.014
0.748MetAsp: 0.748 ± 0.033
0.787MetGlu: 0.787 ± 0.036
0.642MetPhe: 0.642 ± 0.032
1.371MetGly: 1.371 ± 0.051
0.373MetHis: 0.373 ± 0.025
0.995MetIle: 0.995 ± 0.04
0.608MetLys: 0.608 ± 0.027
2.186MetLeu: 2.186 ± 0.061
0.38MetMet: 0.38 ± 0.025
0.642MetAsn: 0.642 ± 0.029
1.097MetPro: 1.097 ± 0.039
0.707MetGln: 0.707 ± 0.029
1.412MetArg: 1.412 ± 0.045
1.584MetSer: 1.584 ± 0.051
1.595MetThr: 1.595 ± 0.046
1.46MetVal: 1.46 ± 0.046
0.2MetTrp: 0.2 ± 0.016
0.359MetTyr: 0.359 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
2.874AsnAla: 2.874 ± 0.066
0.192AsnCys: 0.192 ± 0.017
1.41AsnAsp: 1.41 ± 0.046
1.46AsnGlu: 1.46 ± 0.045
0.826AsnPhe: 0.826 ± 0.032
2.676AsnGly: 2.676 ± 0.074
0.409AsnHis: 0.409 ± 0.022
1.126AsnIle: 1.126 ± 0.043
0.706AsnLys: 0.706 ± 0.034
2.306AsnLeu: 2.306 ± 0.061
0.458AsnMet: 0.458 ± 0.027
0.771AsnAsn: 0.771 ± 0.041
1.969AsnPro: 1.969 ± 0.049
0.762AsnGln: 0.762 ± 0.037
1.591AsnArg: 1.591 ± 0.049
1.654AsnSer: 1.654 ± 0.052
1.853AsnThr: 1.853 ± 0.069
2.101AsnVal: 2.101 ± 0.069
0.433AsnTrp: 0.433 ± 0.023
0.674AsnTyr: 0.674 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
5.971ProAla: 5.971 ± 0.112
0.233ProCys: 0.233 ± 0.021
3.057ProAsp: 3.057 ± 0.076
4.005ProGlu: 4.005 ± 0.096
1.704ProPhe: 1.704 ± 0.048
4.738ProGly: 4.738 ± 0.1
0.982ProHis: 0.982 ± 0.038
2.204ProIle: 2.204 ± 0.056
1.687ProLys: 1.687 ± 0.069
4.702ProLeu: 4.702 ± 0.084
0.911ProMet: 0.911 ± 0.036
1.48ProAsn: 1.48 ± 0.044
1.867ProPro: 1.867 ± 0.063
1.722ProGln: 1.722 ± 0.054
2.764ProArg: 2.764 ± 0.062
3.241ProSer: 3.241 ± 0.076
3.294ProThr: 3.294 ± 0.079
4.575ProVal: 4.575 ± 0.092
0.665ProTrp: 0.665 ± 0.028
0.961ProTyr: 0.961 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
3.724GlnAla: 3.724 ± 0.072
0.141GlnCys: 0.141 ± 0.013
1.451GlnAsp: 1.451 ± 0.048
1.557GlnGlu: 1.557 ± 0.045
1.051GlnPhe: 1.051 ± 0.038
2.409GlnGly: 2.409 ± 0.062
0.799GlnHis: 0.799 ± 0.033
1.729GlnIle: 1.729 ± 0.053
0.961GlnLys: 0.961 ± 0.04
3.742GlnLeu: 3.742 ± 0.074
0.618GlnMet: 0.618 ± 0.03
0.904GlnAsn: 0.904 ± 0.036
1.686GlnPro: 1.686 ± 0.054
1.554GlnGln: 1.554 ± 0.056
2.658GlnArg: 2.658 ± 0.073
1.914GlnSer: 1.914 ± 0.057
1.867GlnThr: 1.867 ± 0.048
2.564GlnVal: 2.564 ± 0.064
0.407GlnTrp: 0.407 ± 0.026
0.685GlnTyr: 0.685 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
7.382ArgAla: 7.382 ± 0.132
0.419ArgCys: 0.419 ± 0.025
3.627ArgAsp: 3.627 ± 0.083
4.328ArgGlu: 4.328 ± 0.105
2.307ArgPhe: 2.307 ± 0.064
4.93ArgGly: 4.93 ± 0.104
1.254ArgHis: 1.254 ± 0.045
3.474ArgIle: 3.474 ± 0.079
1.79ArgLys: 1.79 ± 0.051
6.374ArgLeu: 6.374 ± 0.134
1.526ArgMet: 1.526 ± 0.042
1.55ArgAsn: 1.55 ± 0.052
2.741ArgPro: 2.741 ± 0.068
2.118ArgGln: 2.118 ± 0.067
4.952ArgArg: 4.952 ± 0.109
4.108ArgSer: 4.108 ± 0.081
3.62ArgThr: 3.62 ± 0.069
5.632ArgVal: 5.632 ± 0.104
0.979ArgTrp: 0.979 ± 0.04
1.495ArgTyr: 1.495 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
7.453SerAla: 7.453 ± 0.111
0.423SerCys: 0.423 ± 0.026
3.578SerAsp: 3.578 ± 0.074
3.751SerGlu: 3.751 ± 0.085
2.347SerPhe: 2.347 ± 0.057
6.847SerGly: 6.847 ± 0.15
1.183SerHis: 1.183 ± 0.044
2.992SerIle: 2.992 ± 0.069
1.841SerLys: 1.841 ± 0.051
5.994SerLeu: 5.994 ± 0.087
1.303SerMet: 1.303 ± 0.045
1.767SerAsn: 1.767 ± 0.057
3.293SerPro: 3.293 ± 0.07
1.824SerGln: 1.824 ± 0.058
4.057SerArg: 4.057 ± 0.082
4.306SerSer: 4.306 ± 0.101
3.93SerThr: 3.93 ± 0.098
5.329SerVal: 5.329 ± 0.112
0.972SerTrp: 0.972 ± 0.038
1.406SerTyr: 1.406 ± 0.047
0.0SerXaa: 0.0 ± 0.0
Thr
7.556ThrAla: 7.556 ± 0.167
0.395ThrCys: 0.395 ± 0.037
3.3ThrAsp: 3.3 ± 0.085
3.543ThrGlu: 3.543 ± 0.076
2.012ThrPhe: 2.012 ± 0.055
6.019ThrGly: 6.019 ± 0.138
1.252ThrHis: 1.252 ± 0.043
3.229ThrIle: 3.229 ± 0.075
1.724ThrLys: 1.724 ± 0.051
6.63ThrLeu: 6.63 ± 0.115
1.089ThrMet: 1.089 ± 0.038
1.76ThrAsn: 1.76 ± 0.067
4.108ThrPro: 4.108 ± 0.102
1.925ThrGln: 1.925 ± 0.05
3.564ThrArg: 3.564 ± 0.079
3.912ThrSer: 3.912 ± 0.088
4.076ThrThr: 4.076 ± 0.119
6.39ThrVal: 6.39 ± 0.196
0.799ThrTrp: 0.799 ± 0.038
1.284ThrTyr: 1.284 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
10.706ValAla: 10.706 ± 0.155
0.599ValCys: 0.599 ± 0.025
4.707ValAsp: 4.707 ± 0.085
4.8ValGlu: 4.8 ± 0.096
3.025ValPhe: 3.025 ± 0.073
6.734ValGly: 6.734 ± 0.107
1.38ValHis: 1.38 ± 0.048
4.348ValIle: 4.348 ± 0.083
2.264ValLys: 2.264 ± 0.075
8.923ValLeu: 8.923 ± 0.137
1.643ValMet: 1.643 ± 0.053
2.233ValAsn: 2.233 ± 0.059
4.186ValPro: 4.186 ± 0.093
2.546ValGln: 2.546 ± 0.061
5.251ValArg: 5.251 ± 0.096
6.017ValSer: 6.017 ± 0.121
6.353ValThr: 6.353 ± 0.215
7.92ValVal: 7.92 ± 0.145
1.107ValTrp: 1.107 ± 0.046
1.561ValTyr: 1.561 ± 0.05
0.0ValXaa: 0.0 ± 0.0
Trp
1.371TrpAla: 1.371 ± 0.043
0.103TrpCys: 0.103 ± 0.013
0.672TrpAsp: 0.672 ± 0.03
0.62TrpGlu: 0.62 ± 0.034
0.572TrpPhe: 0.572 ± 0.034
0.998TrpGly: 0.998 ± 0.044
0.298TrpHis: 0.298 ± 0.02
0.778TrpIle: 0.778 ± 0.044
0.352TrpLys: 0.352 ± 0.026
1.495TrpLeu: 1.495 ± 0.046
0.405TrpMet: 0.405 ± 0.027
0.45TrpAsn: 0.45 ± 0.025
0.647TrpPro: 0.647 ± 0.033
0.601TrpGln: 0.601 ± 0.033
0.995TrpArg: 0.995 ± 0.037
0.947TrpSer: 0.947 ± 0.036
0.709TrpThr: 0.709 ± 0.034
1.188TrpVal: 1.188 ± 0.044
0.308TrpTrp: 0.308 ± 0.022
0.265TrpTyr: 0.265 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.176TyrAla: 2.176 ± 0.055
0.161TyrCys: 0.161 ± 0.015
1.257TyrAsp: 1.257 ± 0.046
1.156TyrGlu: 1.156 ± 0.04
0.755TyrPhe: 0.755 ± 0.036
1.789TyrGly: 1.789 ± 0.049
0.32TyrHis: 0.32 ± 0.022
0.723TyrIle: 0.723 ± 0.036
0.558TyrLys: 0.558 ± 0.039
2.102TyrLeu: 2.102 ± 0.06
0.304TyrMet: 0.304 ± 0.021
0.508TyrAsn: 0.508 ± 0.029
0.979TyrPro: 0.979 ± 0.04
0.678TyrGln: 0.678 ± 0.031
1.682TyrArg: 1.682 ± 0.057
1.399TyrSer: 1.399 ± 0.044
1.392TyrThr: 1.392 ± 0.057
1.576TyrVal: 1.576 ± 0.047
0.329TyrTrp: 0.329 ± 0.023
0.451TyrTyr: 0.451 ± 0.031
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2376 proteins (718273 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski