Ectothiorhodospira haloalkaliphila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Ectothiorhodospira

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2964 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8KMX4|W8KMX4_9GAMM Cro/Cl family transcriptional regulator OS=Ectothiorhodospira haloalkaliphila OX=421628 GN=M911_16870 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84VAIYY6 pKa = 10.07FGTTSGSTLHH16 pKa = 5.44VAEE19 pKa = 5.35MIRR22 pKa = 11.84DD23 pKa = 3.76ALQPMSVDD31 pKa = 3.76LLDD34 pKa = 4.64VATLDD39 pKa = 4.06NAHH42 pKa = 6.64SMTDD46 pKa = 3.32YY47 pKa = 11.67DD48 pKa = 4.31LLIWGAPTWGYY59 pKa = 11.76GDD61 pKa = 5.45LQDD64 pKa = 4.71DD65 pKa = 3.83WEE67 pKa = 4.75DD68 pKa = 5.14FIDD71 pKa = 4.9HH72 pKa = 7.28LDD74 pKa = 4.07DD75 pKa = 4.02VDD77 pKa = 5.57LSGLPVALFGLGDD90 pKa = 3.31QYY92 pKa = 11.74GYY94 pKa = 11.72GEE96 pKa = 4.43VFVDD100 pKa = 4.7AMRR103 pKa = 11.84ILHH106 pKa = 7.01DD107 pKa = 3.64KK108 pKa = 10.05AQARR112 pKa = 11.84GARR115 pKa = 11.84ILGYY119 pKa = 9.85WPIDD123 pKa = 3.17EE124 pKa = 5.82HH125 pKa = 9.09YY126 pKa = 11.09DD127 pKa = 3.71FEE129 pKa = 6.41HH130 pKa = 6.46SLAVIDD136 pKa = 5.31DD137 pKa = 4.45HH138 pKa = 7.49FCGLALDD145 pKa = 4.89EE146 pKa = 5.43DD147 pKa = 4.32NQSEE151 pKa = 4.44FTDD154 pKa = 3.22ARR156 pKa = 11.84VATWCRR162 pKa = 11.84QLQAVVFPP170 pKa = 4.96

Molecular weight: 19.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8L291|W8L291_9GAMM Biosynthetic arginine decarboxylase OS=Ectothiorhodospira haloalkaliphila OX=421628 GN=speA PE=3 SV=1
MM1 pKa = 7.58RR2 pKa = 11.84PWRR5 pKa = 11.84TLPAPRR11 pKa = 11.84PRR13 pKa = 11.84WRR15 pKa = 11.84FIWRR19 pKa = 11.84FIVWRR24 pKa = 11.84RR25 pKa = 11.84WWSAWNRR32 pKa = 11.84FMGRR36 pKa = 11.84IVRR39 pKa = 11.84QRR41 pKa = 11.84WWRR44 pKa = 11.84APPGRR49 pKa = 11.84TRR51 pKa = 11.84WWCVAPWGIWWRR63 pKa = 3.76

Molecular weight: 8.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2964

0

2964

929654

36

1837

313.6

34.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.329 ± 0.045

0.932 ± 0.014

6.007 ± 0.034

6.628 ± 0.045

3.369 ± 0.028

8.238 ± 0.042

2.639 ± 0.023

4.523 ± 0.032

2.618 ± 0.036

11.05 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.646 ± 0.02

2.501 ± 0.026

5.256 ± 0.035

4.17 ± 0.032

7.973 ± 0.046

5.071 ± 0.03

4.915 ± 0.027

7.383 ± 0.041

1.439 ± 0.022

2.312 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski