Lutispora thermophila DSM 19022

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Lutispora; Lutispora thermophila

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3409 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1M6H2B0|A0A1M6H2B0_9CLOT Uncharacterized protein OS=Lutispora thermophila DSM 19022 OX=1122184 GN=SAMN02745176_02615 PE=4 SV=1
MM1 pKa = 7.3QIIEE5 pKa = 4.15GLYY8 pKa = 9.21YY9 pKa = 10.87SKK11 pKa = 10.93DD12 pKa = 3.67HH13 pKa = 6.38EE14 pKa = 4.54WVKK17 pKa = 11.07VEE19 pKa = 3.84GDD21 pKa = 3.19KK22 pKa = 11.17AYY24 pKa = 11.24VGISDD29 pKa = 4.61FAQDD33 pKa = 3.56ALGDD37 pKa = 3.75IVFVEE42 pKa = 4.55LPEE45 pKa = 5.67IGTNLIQGEE54 pKa = 4.28ALGSIEE60 pKa = 4.3SVKK63 pKa = 10.58AASDD67 pKa = 3.93VYY69 pKa = 10.92CPVSGKK75 pKa = 9.78VIKK78 pKa = 10.87VNEE81 pKa = 4.15ILVDD85 pKa = 4.69DD86 pKa = 4.58PALLNEE92 pKa = 4.68DD93 pKa = 4.6PYY95 pKa = 10.05EE96 pKa = 3.8NWMICVEE103 pKa = 4.2LTEE106 pKa = 5.46KK107 pKa = 10.96DD108 pKa = 3.87QLDD111 pKa = 3.57QLMNSAEE118 pKa = 4.19YY119 pKa = 10.81EE120 pKa = 4.14DD121 pKa = 4.41FCNEE125 pKa = 3.74EE126 pKa = 3.95AA127 pKa = 4.93

Molecular weight:
14.18 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1M6EVL7|A0A1M6EVL7_9CLOT L-fuculose-phosphate aldolase OS=Lutispora thermophila DSM 19022 OX=1122184 GN=SAMN02745176_01724 PE=4 SV=1
MM1 pKa = 7.79LILTVTCFFRR11 pKa = 11.84IQRR14 pKa = 11.84PPTWKK19 pKa = 8.49TRR21 pKa = 11.84PFEE24 pKa = 3.91VLARR28 pKa = 11.84MVILRR33 pKa = 11.84FLAPNWCVDD42 pKa = 3.22AYY44 pKa = 10.23ILFVPTANPEE54 pKa = 3.96ASDD57 pKa = 3.62FDD59 pKa = 4.42LMEE62 pKa = 4.49FLSPAVSTSVTPPSCTALMDD82 pKa = 4.18CFHH85 pKa = 7.38FFPSGPFSVRR95 pKa = 11.84NRR97 pKa = 11.84STVDD101 pKa = 2.76SRR103 pKa = 11.84TASAIAALMPFLSMSASCRR122 pKa = 11.84SLFISLLVSPSSLMLYY138 pKa = 9.81IYY140 pKa = 10.37IPFRR144 pKa = 11.84LSRR147 pKa = 11.84EE148 pKa = 3.7KK149 pKa = 10.73RR150 pKa = 11.84RR151 pKa = 11.84GLDD154 pKa = 3.15ATEE157 pKa = 4.28KK158 pKa = 9.33PVRR161 pKa = 11.84AAVVVSTAPISTFPSASNSKK181 pKa = 10.01NRR183 pKa = 11.84IAALYY188 pKa = 9.81RR189 pKa = 11.84FRR191 pKa = 6.04

Molecular weight:
21.36 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3409

0

3409

1016382

39

2664

298.1

33.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.373 ± 0.05

1.14 ± 0.018

5.736 ± 0.031

7.465 ± 0.042

4.113 ± 0.029

6.567 ± 0.042

1.491 ± 0.017

9.654 ± 0.049

8.236 ± 0.041

8.991 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.941 ± 0.022

5.385 ± 0.04

3.101 ± 0.025

2.58 ± 0.024

3.963 ± 0.029

6.059 ± 0.033

4.73 ± 0.029

6.495 ± 0.031

0.75 ± 0.013

4.23 ± 0.031

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski