Amino acid dipepetide frequency for Leuconostoc phage LN25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.164AlaAla: 1.164 ± 0.542
0.233AlaCys: 0.233 ± 0.178
4.538AlaAsp: 4.538 ± 1.023
2.909AlaGlu: 2.909 ± 0.554
3.374AlaPhe: 3.374 ± 0.846
4.887AlaGly: 4.887 ± 0.911
1.047AlaHis: 1.047 ± 0.364
6.283AlaIle: 6.283 ± 1.035
3.956AlaLys: 3.956 ± 0.722
6.167AlaLeu: 6.167 ± 0.787
1.28AlaMet: 1.28 ± 0.277
5.236AlaAsn: 5.236 ± 1.104
1.745AlaPro: 1.745 ± 0.422
1.745AlaGln: 1.745 ± 0.465
1.28AlaArg: 1.28 ± 0.537
3.956AlaSer: 3.956 ± 1.013
4.305AlaThr: 4.305 ± 0.721
3.84AlaVal: 3.84 ± 0.556
1.164AlaTrp: 1.164 ± 0.41
2.327AlaTyr: 2.327 ± 0.319
0.0AlaXaa: 0.0 ± 0.0
Cys
0.233CysAla: 0.233 ± 0.178
0.0CysCys: 0.0 ± 0.0
0.0CysAsp: 0.0 ± 0.0
0.233CysGlu: 0.233 ± 0.178
0.233CysPhe: 0.233 ± 0.16
0.349CysGly: 0.349 ± 0.225
0.349CysHis: 0.349 ± 0.204
0.233CysIle: 0.233 ± 0.165
0.233CysLys: 0.233 ± 0.17
0.116CysLeu: 0.116 ± 0.143
0.116CysMet: 0.116 ± 0.124
0.116CysAsn: 0.116 ± 0.133
0.116CysPro: 0.116 ± 0.113
0.116CysGln: 0.116 ± 0.13
0.0CysArg: 0.0 ± 0.0
0.233CysSer: 0.233 ± 0.194
0.0CysThr: 0.0 ± 0.0
0.116CysVal: 0.116 ± 0.108
0.116CysTrp: 0.116 ± 0.127
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
3.724AspAla: 3.724 ± 0.803
0.116AspCys: 0.116 ± 0.133
4.538AspAsp: 4.538 ± 0.975
5.353AspGlu: 5.353 ± 1.063
4.189AspPhe: 4.189 ± 0.689
4.305AspGly: 4.305 ± 0.833
1.396AspHis: 1.396 ± 0.412
5.003AspIle: 5.003 ± 0.827
4.654AspLys: 4.654 ± 0.892
5.353AspLeu: 5.353 ± 0.628
1.396AspMet: 1.396 ± 0.38
4.073AspAsn: 4.073 ± 0.825
1.745AspPro: 1.745 ± 0.546
1.047AspGln: 1.047 ± 0.33
1.978AspArg: 1.978 ± 0.402
3.025AspSer: 3.025 ± 0.53
4.422AspThr: 4.422 ± 0.765
4.073AspVal: 4.073 ± 0.783
1.047AspTrp: 1.047 ± 0.377
4.189AspTyr: 4.189 ± 1.049
0.0AspXaa: 0.0 ± 0.0
Glu
2.676GluAla: 2.676 ± 0.714
0.116GluCys: 0.116 ± 0.121
3.724GluAsp: 3.724 ± 0.861
4.073GluGlu: 4.073 ± 0.948
2.793GluPhe: 2.793 ± 0.579
2.793GluGly: 2.793 ± 0.523
1.28GluHis: 1.28 ± 0.339
4.538GluIle: 4.538 ± 0.872
5.353GluLys: 5.353 ± 0.945
5.934GluLeu: 5.934 ± 0.787
2.793GluMet: 2.793 ± 0.807
4.073GluAsn: 4.073 ± 0.715
1.629GluPro: 1.629 ± 0.396
2.793GluGln: 2.793 ± 0.767
2.094GluArg: 2.094 ± 0.658
2.327GluSer: 2.327 ± 0.591
3.258GluThr: 3.258 ± 0.553
4.887GluVal: 4.887 ± 0.84
0.465GluTrp: 0.465 ± 0.205
3.491GluTyr: 3.491 ± 0.629
0.0GluXaa: 0.0 ± 0.0
Phe
2.327PheAla: 2.327 ± 0.509
0.0PheCys: 0.0 ± 0.0
3.607PheAsp: 3.607 ± 0.552
3.374PheGlu: 3.374 ± 0.734
1.513PhePhe: 1.513 ± 0.402
3.258PheGly: 3.258 ± 0.757
0.349PheHis: 0.349 ± 0.214
4.887PheIle: 4.887 ± 0.66
2.676PheLys: 2.676 ± 0.593
3.607PheLeu: 3.607 ± 0.842
0.815PheMet: 0.815 ± 0.299
3.142PheAsn: 3.142 ± 0.609
1.047PhePro: 1.047 ± 0.336
0.931PheGln: 0.931 ± 0.4
1.745PheArg: 1.745 ± 0.461
3.84PheSer: 3.84 ± 0.733
3.724PheThr: 3.724 ± 0.53
2.909PheVal: 2.909 ± 0.581
0.582PheTrp: 0.582 ± 0.351
1.745PheTyr: 1.745 ± 0.48
0.0PheXaa: 0.0 ± 0.0
Gly
4.189GlyAla: 4.189 ± 1.093
0.0GlyCys: 0.0 ± 0.0
5.12GlyAsp: 5.12 ± 0.759
3.491GlyGlu: 3.491 ± 0.563
3.724GlyPhe: 3.724 ± 0.676
6.749GlyGly: 6.749 ± 1.141
0.698GlyHis: 0.698 ± 0.257
5.236GlyIle: 5.236 ± 0.852
6.167GlyLys: 6.167 ± 0.942
5.236GlyLeu: 5.236 ± 0.78
2.094GlyMet: 2.094 ± 0.449
4.771GlyAsn: 4.771 ± 1.027
0.116GlyPro: 0.116 ± 0.114
1.745GlyGln: 1.745 ± 0.412
2.444GlyArg: 2.444 ± 0.537
6.051GlySer: 6.051 ± 1.153
3.258GlyThr: 3.258 ± 0.715
5.353GlyVal: 5.353 ± 1.338
0.465GlyTrp: 0.465 ± 0.226
3.142GlyTyr: 3.142 ± 0.912
0.0GlyXaa: 0.0 ± 0.0
His
0.698HisAla: 0.698 ± 0.328
0.0HisCys: 0.0 ± 0.0
1.047HisAsp: 1.047 ± 0.297
0.931HisGlu: 0.931 ± 0.333
0.582HisPhe: 0.582 ± 0.248
0.698HisGly: 0.698 ± 0.289
0.233HisHis: 0.233 ± 0.161
1.047HisIle: 1.047 ± 0.302
0.465HisLys: 0.465 ± 0.231
1.28HisLeu: 1.28 ± 0.499
1.164HisMet: 1.164 ± 0.398
0.815HisAsn: 0.815 ± 0.378
0.582HisPro: 0.582 ± 0.265
0.582HisGln: 0.582 ± 0.283
0.465HisArg: 0.465 ± 0.235
0.815HisSer: 0.815 ± 0.322
1.164HisThr: 1.164 ± 0.475
0.815HisVal: 0.815 ± 0.337
0.116HisTrp: 0.116 ± 0.151
0.815HisTyr: 0.815 ± 0.306
0.0HisXaa: 0.0 ± 0.0
Ile
4.538IleAla: 4.538 ± 0.766
0.116IleCys: 0.116 ± 0.143
4.771IleAsp: 4.771 ± 0.732
5.702IleGlu: 5.702 ± 0.955
2.676IlePhe: 2.676 ± 0.562
6.051IleGly: 6.051 ± 1.398
0.931IleHis: 0.931 ± 0.319
5.12IleIle: 5.12 ± 0.777
5.585IleLys: 5.585 ± 0.936
4.538IleLeu: 4.538 ± 0.643
2.444IleMet: 2.444 ± 0.558
5.353IleAsn: 5.353 ± 0.77
2.211IlePro: 2.211 ± 0.548
2.909IleGln: 2.909 ± 0.607
1.862IleArg: 1.862 ± 0.436
4.422IleSer: 4.422 ± 0.764
6.167IleThr: 6.167 ± 1.001
4.538IleVal: 4.538 ± 0.704
0.349IleTrp: 0.349 ± 0.174
2.793IleTyr: 2.793 ± 0.633
0.0IleXaa: 0.0 ± 0.0
Lys
5.934LysAla: 5.934 ± 0.72
0.465LysCys: 0.465 ± 0.319
4.073LysAsp: 4.073 ± 0.662
4.538LysGlu: 4.538 ± 1.036
4.073LysPhe: 4.073 ± 0.615
4.422LysGly: 4.422 ± 0.619
1.164LysHis: 1.164 ± 0.448
5.12LysIle: 5.12 ± 0.831
6.633LysLys: 6.633 ± 1.031
6.283LysLeu: 6.283 ± 1.014
3.142LysMet: 3.142 ± 0.683
3.491LysAsn: 3.491 ± 0.543
2.211LysPro: 2.211 ± 0.488
2.793LysGln: 2.793 ± 0.422
3.491LysArg: 3.491 ± 0.752
4.538LysSer: 4.538 ± 0.639
5.702LysThr: 5.702 ± 0.968
3.956LysVal: 3.956 ± 0.669
0.931LysTrp: 0.931 ± 0.295
3.724LysTyr: 3.724 ± 0.71
0.0LysXaa: 0.0 ± 0.0
Leu
4.887LeuAla: 4.887 ± 0.94
0.233LeuCys: 0.233 ± 0.175
6.4LeuAsp: 6.4 ± 0.753
4.887LeuGlu: 4.887 ± 0.863
3.374LeuPhe: 3.374 ± 0.551
5.353LeuGly: 5.353 ± 0.811
1.164LeuHis: 1.164 ± 0.321
4.422LeuIle: 4.422 ± 0.847
8.145LeuLys: 8.145 ± 0.982
4.654LeuLeu: 4.654 ± 0.728
2.444LeuMet: 2.444 ± 0.48
5.469LeuAsn: 5.469 ± 0.613
2.909LeuPro: 2.909 ± 0.629
3.258LeuGln: 3.258 ± 0.522
2.211LeuArg: 2.211 ± 0.403
7.331LeuSer: 7.331 ± 0.882
5.469LeuThr: 5.469 ± 0.782
5.353LeuVal: 5.353 ± 0.886
0.582LeuTrp: 0.582 ± 0.251
2.211LeuTyr: 2.211 ± 0.437
0.0LeuXaa: 0.0 ± 0.0
Met
2.327MetAla: 2.327 ± 0.483
0.116MetCys: 0.116 ± 0.127
1.047MetAsp: 1.047 ± 0.288
0.698MetGlu: 0.698 ± 0.254
1.047MetPhe: 1.047 ± 0.432
1.28MetGly: 1.28 ± 0.344
0.349MetHis: 0.349 ± 0.261
1.396MetIle: 1.396 ± 0.41
2.327MetLys: 2.327 ± 0.479
2.327MetLeu: 2.327 ± 0.468
0.815MetMet: 0.815 ± 0.298
3.142MetAsn: 3.142 ± 0.674
0.698MetPro: 0.698 ± 0.312
0.465MetGln: 0.465 ± 0.183
0.698MetArg: 0.698 ± 0.268
2.444MetSer: 2.444 ± 0.438
2.676MetThr: 2.676 ± 0.588
1.978MetVal: 1.978 ± 0.508
0.233MetTrp: 0.233 ± 0.179
1.629MetTyr: 1.629 ± 0.453
0.0MetXaa: 0.0 ± 0.0
Asn
3.84AsnAla: 3.84 ± 0.724
0.116AsnCys: 0.116 ± 0.12
3.607AsnAsp: 3.607 ± 0.769
5.236AsnGlu: 5.236 ± 0.78
2.211AsnPhe: 2.211 ± 0.655
4.771AsnGly: 4.771 ± 0.993
0.582AsnHis: 0.582 ± 0.344
5.236AsnIle: 5.236 ± 0.761
4.305AsnLys: 4.305 ± 0.719
5.236AsnLeu: 5.236 ± 0.934
1.396AsnMet: 1.396 ± 0.408
4.887AsnAsn: 4.887 ± 0.733
3.025AsnPro: 3.025 ± 0.672
2.444AsnGln: 2.444 ± 0.381
3.258AsnArg: 3.258 ± 0.787
4.189AsnSer: 4.189 ± 0.565
4.422AsnThr: 4.422 ± 0.807
4.654AsnVal: 4.654 ± 0.667
0.815AsnTrp: 0.815 ± 0.298
2.909AsnTyr: 2.909 ± 0.675
0.0AsnXaa: 0.0 ± 0.0
Pro
1.629ProAla: 1.629 ± 0.39
0.0ProCys: 0.0 ± 0.0
2.094ProAsp: 2.094 ± 0.401
1.745ProGlu: 1.745 ± 0.56
1.745ProPhe: 1.745 ± 0.434
0.931ProGly: 0.931 ± 0.342
0.582ProHis: 0.582 ± 0.256
1.978ProIle: 1.978 ± 0.483
2.793ProLys: 2.793 ± 0.569
1.745ProLeu: 1.745 ± 0.321
0.815ProMet: 0.815 ± 0.548
2.211ProAsn: 2.211 ± 0.647
0.582ProPro: 0.582 ± 0.24
1.164ProGln: 1.164 ± 0.47
1.047ProArg: 1.047 ± 0.474
1.978ProSer: 1.978 ± 0.438
2.211ProThr: 2.211 ± 0.51
1.745ProVal: 1.745 ± 0.47
0.0ProTrp: 0.0 ± 0.0
0.931ProTyr: 0.931 ± 0.339
0.0ProXaa: 0.0 ± 0.0
Gln
2.676GlnAla: 2.676 ± 0.623
0.0GlnCys: 0.0 ± 0.0
1.745GlnAsp: 1.745 ± 0.461
2.094GlnGlu: 2.094 ± 0.495
1.629GlnPhe: 1.629 ± 0.432
1.629GlnGly: 1.629 ± 0.415
0.233GlnHis: 0.233 ± 0.165
2.327GlnIle: 2.327 ± 0.481
2.676GlnLys: 2.676 ± 0.609
3.258GlnLeu: 3.258 ± 0.685
0.931GlnMet: 0.931 ± 0.332
2.56GlnAsn: 2.56 ± 0.503
1.396GlnPro: 1.396 ± 0.418
0.815GlnGln: 0.815 ± 0.316
1.513GlnArg: 1.513 ± 0.417
2.211GlnSer: 2.211 ± 0.497
1.978GlnThr: 1.978 ± 0.472
2.56GlnVal: 2.56 ± 0.438
0.349GlnTrp: 0.349 ± 0.184
1.164GlnTyr: 1.164 ± 0.539
0.0GlnXaa: 0.0 ± 0.0
Arg
2.327ArgAla: 2.327 ± 0.499
0.349ArgCys: 0.349 ± 0.273
2.793ArgAsp: 2.793 ± 0.615
1.862ArgGlu: 1.862 ± 0.542
1.164ArgPhe: 1.164 ± 0.435
1.513ArgGly: 1.513 ± 0.54
0.582ArgHis: 0.582 ± 0.268
1.862ArgIle: 1.862 ± 0.446
2.327ArgLys: 2.327 ± 0.514
5.003ArgLeu: 5.003 ± 0.762
0.698ArgMet: 0.698 ± 0.263
2.094ArgAsn: 2.094 ± 0.64
0.815ArgPro: 0.815 ± 0.362
1.396ArgGln: 1.396 ± 0.384
0.815ArgArg: 0.815 ± 0.357
2.211ArgSer: 2.211 ± 0.492
2.56ArgThr: 2.56 ± 0.633
2.327ArgVal: 2.327 ± 0.435
0.698ArgTrp: 0.698 ± 0.228
1.164ArgTyr: 1.164 ± 0.4
0.0ArgXaa: 0.0 ± 0.0
Ser
5.003SerAla: 5.003 ± 0.929
0.0SerCys: 0.0 ± 0.0
4.538SerAsp: 4.538 ± 0.79
3.84SerGlu: 3.84 ± 0.805
3.491SerPhe: 3.491 ± 0.558
5.818SerGly: 5.818 ± 1.473
0.815SerHis: 0.815 ± 0.336
5.702SerIle: 5.702 ± 0.79
4.538SerLys: 4.538 ± 0.617
4.538SerLeu: 4.538 ± 0.62
1.513SerMet: 1.513 ± 0.428
3.724SerAsn: 3.724 ± 0.64
1.396SerPro: 1.396 ± 0.413
2.327SerGln: 2.327 ± 0.423
2.094SerArg: 2.094 ± 0.59
5.12SerSer: 5.12 ± 1.384
5.585SerThr: 5.585 ± 0.895
4.654SerVal: 4.654 ± 0.813
0.698SerTrp: 0.698 ± 0.242
2.676SerTyr: 2.676 ± 0.528
0.0SerXaa: 0.0 ± 0.0
Thr
5.702ThrAla: 5.702 ± 1.172
0.349ThrCys: 0.349 ± 0.197
3.491ThrAsp: 3.491 ± 0.581
3.025ThrGlu: 3.025 ± 0.585
3.491ThrPhe: 3.491 ± 0.705
5.702ThrGly: 5.702 ± 0.815
1.047ThrHis: 1.047 ± 0.393
4.305ThrIle: 4.305 ± 0.96
6.051ThrLys: 6.051 ± 0.714
5.702ThrLeu: 5.702 ± 0.911
1.745ThrMet: 1.745 ± 0.583
3.956ThrAsn: 3.956 ± 0.619
2.444ThrPro: 2.444 ± 0.551
3.491ThrGln: 3.491 ± 0.644
2.56ThrArg: 2.56 ± 0.533
5.353ThrSer: 5.353 ± 0.681
6.051ThrThr: 6.051 ± 1.251
3.142ThrVal: 3.142 ± 0.581
0.465ThrTrp: 0.465 ± 0.233
1.978ThrTyr: 1.978 ± 0.66
0.0ThrXaa: 0.0 ± 0.0
Val
4.887ValAla: 4.887 ± 0.978
0.349ValCys: 0.349 ± 0.182
4.305ValAsp: 4.305 ± 0.76
3.025ValGlu: 3.025 ± 0.494
2.56ValPhe: 2.56 ± 0.572
6.051ValGly: 6.051 ± 1.061
0.349ValHis: 0.349 ± 0.223
5.585ValIle: 5.585 ± 0.732
4.073ValLys: 4.073 ± 0.562
4.654ValLeu: 4.654 ± 0.655
1.047ValMet: 1.047 ± 0.271
4.422ValAsn: 4.422 ± 0.695
1.745ValPro: 1.745 ± 0.469
1.513ValGln: 1.513 ± 0.465
3.142ValArg: 3.142 ± 0.867
4.305ValSer: 4.305 ± 0.79
4.422ValThr: 4.422 ± 0.675
4.073ValVal: 4.073 ± 0.902
1.28ValTrp: 1.28 ± 0.451
3.258ValTyr: 3.258 ± 0.651
0.0ValXaa: 0.0 ± 0.0
Trp
0.233TrpAla: 0.233 ± 0.167
0.0TrpCys: 0.0 ± 0.0
0.815TrpAsp: 0.815 ± 0.28
0.582TrpGlu: 0.582 ± 0.294
0.349TrpPhe: 0.349 ± 0.225
0.815TrpGly: 0.815 ± 0.313
0.698TrpHis: 0.698 ± 0.291
0.116TrpIle: 0.116 ± 0.131
0.465TrpLys: 0.465 ± 0.253
1.164TrpLeu: 1.164 ± 0.398
0.233TrpMet: 0.233 ± 0.182
0.931TrpAsn: 0.931 ± 0.373
0.233TrpPro: 0.233 ± 0.141
0.582TrpGln: 0.582 ± 0.221
0.582TrpArg: 0.582 ± 0.306
0.815TrpSer: 0.815 ± 0.293
0.465TrpThr: 0.465 ± 0.221
0.931TrpVal: 0.931 ± 0.302
0.233TrpTrp: 0.233 ± 0.181
0.465TrpTyr: 0.465 ± 0.246
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.444TyrAla: 2.444 ± 0.434
0.349TyrCys: 0.349 ± 0.203
3.374TyrAsp: 3.374 ± 0.598
3.491TyrGlu: 3.491 ± 0.721
1.862TyrPhe: 1.862 ± 0.546
2.676TyrGly: 2.676 ± 0.652
0.465TyrHis: 0.465 ± 0.256
2.793TyrIle: 2.793 ± 0.487
3.258TyrLys: 3.258 ± 0.699
3.956TyrLeu: 3.956 ± 0.726
0.698TyrMet: 0.698 ± 0.291
2.676TyrAsn: 2.676 ± 0.574
1.28TyrPro: 1.28 ± 0.478
1.629TyrGln: 1.629 ± 0.434
1.28TyrArg: 1.28 ± 0.387
2.909TyrSer: 2.909 ± 0.8
2.211TyrThr: 2.211 ± 0.481
3.142TyrVal: 3.142 ± 0.536
0.116TyrTrp: 0.116 ± 0.143
2.444TyrTyr: 2.444 ± 0.604
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 41 proteins (8595 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski