Streptococcus phage Javan291

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A7E4|A0A4D6A7E4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan291 OX=2548097 GN=Javan291_0033 PE=4 SV=1
MM1 pKa = 7.13FKK3 pKa = 10.68YY4 pKa = 9.67WFEE7 pKa = 3.8QQRR10 pKa = 11.84YY11 pKa = 8.59YY12 pKa = 10.24LTSNQFLILDD22 pKa = 4.22DD23 pKa = 4.07EE24 pKa = 4.34WLEE27 pKa = 4.08MVLAFYY33 pKa = 8.09QQRR36 pKa = 11.84DD37 pKa = 3.72EE38 pKa = 5.03LPII41 pKa = 5.26

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A7F8|A0A4D6A7F8_9CAUD Uncharacterized protein OS=Streptococcus phage Javan291 OX=2548097 GN=Javan291_0064 PE=4 SV=1
MM1 pKa = 7.49VDD3 pKa = 3.32VKK5 pKa = 10.88VHH7 pKa = 5.78VDD9 pKa = 3.26VKK11 pKa = 11.09GIEE14 pKa = 4.02QKK16 pKa = 10.83VSSEE20 pKa = 3.7ALAKK24 pKa = 10.57GKK26 pKa = 9.92LAIANQMLLDD36 pKa = 3.8MEE38 pKa = 4.63RR39 pKa = 11.84FVPKK43 pKa = 10.26RR44 pKa = 11.84RR45 pKa = 11.84GDD47 pKa = 3.45LRR49 pKa = 11.84TSGHH53 pKa = 4.54VRR55 pKa = 11.84KK56 pKa = 10.38DD57 pKa = 3.26SVVYY61 pKa = 9.03NVPYY65 pKa = 10.8ARR67 pKa = 11.84IIYY70 pKa = 9.3YY71 pKa = 9.66GRR73 pKa = 11.84KK74 pKa = 8.64RR75 pKa = 11.84KK76 pKa = 10.04GFFSEE81 pKa = 3.79KK82 pKa = 8.59QRR84 pKa = 11.84RR85 pKa = 11.84FFFANKK91 pKa = 9.87DD92 pKa = 3.38KK93 pKa = 11.16LLNQPKK99 pKa = 9.14TPGTGPRR106 pKa = 11.84WDD108 pKa = 3.68KK109 pKa = 11.12KK110 pKa = 10.81AIPLYY115 pKa = 8.57SEE117 pKa = 3.77KK118 pKa = 9.91WAEE121 pKa = 3.85VGLRR125 pKa = 11.84AMGVSKK131 pKa = 11.06

Molecular weight: 15.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

11947

38

1191

178.3

20.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.6 ± 0.768

0.527 ± 0.086

5.985 ± 0.375

8.245 ± 0.545

4.051 ± 0.185

6.68 ± 0.427

1.406 ± 0.167

6.462 ± 0.226

7.676 ± 0.429

8.328 ± 0.307

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.402 ± 0.158

4.721 ± 0.27

2.628 ± 0.187

4.687 ± 0.247

4.779 ± 0.326

6.202 ± 0.643

5.491 ± 0.341

7.148 ± 0.341

1.23 ± 0.123

3.75 ± 0.32
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski